FastQCFastQC Report
Thu 26 May 2016
SRR938495_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938495_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3029597
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131200.4330608988588251No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99310.32779937397614267No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69520.22946946409043842No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA35160.11605503966369124No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA34710.11456969359291021No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA30530.10077247897987753No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65150.049.9219441
GTATCAA109000.044.6271321
TATCAAC143300.033.546532
ATCAACG143850.033.418273
TCAACGC145450.033.0506554
CAACGCA148100.032.4271625
AACGCAG153750.031.2746356
GTACATG138450.030.0514141
TACATGG139800.029.3185672
GTGGTAT26000.027.4326481
ACATGGG146750.026.893213
ACGCAGA178450.026.6297877
CGCAGAG180350.026.2965438
TGGTATC27050.024.4337522
GCAGAGT193800.023.7109979
CATGGGA75150.023.3474794
CATGGGG73000.023.0580734
GAGTACT113050.021.33183512-13
AGGTAAG12500.020.921622
AGTACTT117150.020.90976312-13
Produced by FastQC (version 0.11.5)