##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938495_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3029597 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.04483467603117 31.0 31.0 34.0 28.0 34.0 2 31.070922304187654 31.0 31.0 34.0 27.0 34.0 3 31.11223043856988 31.0 31.0 34.0 27.0 34.0 4 34.78413663599482 37.0 35.0 37.0 32.0 37.0 5 34.759858819506356 37.0 35.0 37.0 32.0 37.0 6 34.7820911494169 37.0 35.0 37.0 32.0 37.0 7 34.68094436322719 37.0 35.0 37.0 30.0 37.0 8 34.65119255135254 37.0 35.0 37.0 30.0 37.0 9 36.123042107580645 39.0 35.0 39.0 31.0 39.0 10-11 35.949696279736216 38.0 35.0 39.0 30.0 39.0 12-13 35.79886582274804 38.0 35.0 39.0 30.0 39.0 14-15 36.7350345607023 39.0 36.0 41.0 30.0 41.0 16-17 36.56318959254317 39.0 36.0 40.5 30.0 41.0 18-19 36.72767615626765 39.0 36.0 41.0 30.0 41.0 20-21 36.717865610508596 39.0 36.0 41.0 30.0 41.0 22-23 36.622957277816155 39.0 36.0 41.0 30.0 41.0 24-25 36.479283053158554 39.0 36.0 41.0 29.5 41.0 26-27 36.263294590006524 39.0 35.0 41.0 29.0 41.0 28-29 36.04486471302949 39.0 35.0 40.0 27.5 41.0 30-31 35.7722274612762 38.0 35.0 40.0 27.0 41.0 32-33 35.48083639507169 38.0 34.0 40.0 27.0 41.0 34-35 35.20472590908956 38.0 34.0 40.0 26.0 41.0 36-37 34.911642868671976 38.0 34.0 40.0 25.0 41.0 38-39 34.74786134921575 38.0 33.5 40.0 24.5 41.0 40-41 34.61939030174641 38.0 33.0 40.0 24.0 41.0 42-43 34.29881102998188 38.0 33.0 40.0 23.5 41.0 44-45 33.917306988355215 38.0 33.0 40.0 22.5 41.0 46-47 33.47365870774232 38.0 32.5 40.0 20.0 41.0 48-49 33.06973056152353 37.0 31.5 40.0 17.5 41.0 50-51 32.3444501034296 36.5 30.5 39.5 15.0 40.5 52-53 32.3549283947667 36.5 31.0 39.0 14.5 40.0 54-55 32.44880985820886 37.0 31.0 40.0 13.0 41.0 56-57 32.114653038011326 36.0 31.0 39.0 8.5 41.0 58-59 31.731929362222104 36.0 30.5 39.0 7.5 41.0 60-61 31.25451751503583 35.0 29.5 39.0 6.0 40.0 62-63 30.679915183438588 35.0 29.0 38.5 2.0 40.0 64-65 30.157068415370098 35.0 28.0 38.0 2.0 40.0 66-67 29.608500404509247 34.0 28.0 38.0 2.0 40.0 68-69 29.00696528284125 34.0 26.5 37.0 2.0 39.5 70-71 28.367894970849257 34.0 26.0 36.5 2.0 39.0 72-73 27.732648929874173 33.0 25.5 36.0 2.0 39.0 74-75 27.05311449014506 33.0 24.0 35.0 2.0 38.0 76-77 26.29302841268987 32.0 21.5 35.0 2.0 37.0 78-79 25.498337237592988 31.5 19.5 35.0 2.0 36.5 80-81 24.79265080471099 31.0 17.5 34.5 2.0 36.0 82-83 24.03834140316352 30.5 9.0 34.0 2.0 35.5 84-85 23.322097955602676 30.0 4.5 34.0 2.0 35.0 86-87 22.535481946938816 29.5 2.0 34.0 2.0 35.0 88-89 21.683183604948116 29.0 2.0 34.0 2.0 35.0 90-91 20.74613405677389 28.0 2.0 34.0 2.0 35.0 92-93 19.786091186385516 26.5 2.0 33.0 2.0 35.0 94-95 18.748449711298235 25.0 2.0 33.0 2.0 34.5 96-97 17.46926439391114 22.0 2.0 32.0 2.0 34.0 98-99 15.935787664167874 6.5 2.0 32.0 2.0 34.0 100-101 12.523633011255292 2.0 2.0 27.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5210022482729322 1101 2 0.34569507943232125 1101 3 0.4215327610185824 1101 4 0.3158127847809169 1101 5 0.4206973250413526 1101 6 0.4874523696100752 1101 7 0.43811147253164506 1101 8 0.5577741125397466 1101 9 0.7336516715820807 1101 10-11 0.6737482925141691 1101 12-13 0.7941799474307842 1101 14-15 0.9198515373584044 1101 16-17 0.9181499731190428 1101 18-19 0.8415030111196344 1101 20-21 0.8463560324069945 1101 22-23 0.8740836548907822 1101 24-25 0.9122162820215749 1101 26-27 0.8178841844597216 1101 28-29 0.941013651504278 1101 30-31 0.9240094738280504 1101 32-33 0.7777678123411107 1101 34-35 0.6759224714331893 1101 36-37 0.8016886865756092 1101 38-39 0.8329129320942172 1101 40-41 0.9392814465662411 1101 42-43 1.0816012589906236 1101 44-45 1.2773495535500246 1101 46-47 1.1051084708347716 1101 48-49 0.991343216539704 1101 50-51 1.035777002299568 1101 52-53 1.086713039757221 1101 54-55 1.0920536326194252 1101 56-57 0.9975694631994898 1101 58-59 1.1157282913901163 1101 60-61 1.2935311077590939 1101 62-63 1.2626220620372983 1101 64-65 0.9740650079435262 1101 66-67 1.0740363334248677 1101 68-69 1.0937998312383783 1101 70-71 1.1531751354583903 1101 72-73 1.42890413122932 1101 74-75 1.139432455508068 1101 76-77 1.0950549059496346 1101 78-79 1.4615989433195367 1101 80-81 1.3434038435037507 1101 82-83 1.4535256400002048 1101 84-85 1.5543596761794483 1101 86-87 1.7610698905880717 1101 88-89 1.7796611774261883 1101 90-91 1.6033603755649715 1101 92-93 1.5833434374406572 1101 94-95 1.4072630799019343 1101 96-97 1.394868667703058 1101 98-99 1.3469608105262765 1101 100-101 0.9626102358245614 1102 1 0.4694314403721904 1102 2 0.40686362144796107 1102 3 0.4904149687502688 1102 4 0.4320884109825016 1102 5 0.377162958512109 1102 6 0.42530097572358017 1102 7 0.35917466864273706 1102 8 0.6039657296525078 1102 9 0.6185571352997457 1102 10-11 0.6736539478798846 1102 12-13 0.6894178633490711 1102 14-15 0.7640828975686347 1102 16-17 0.9392036204503214 1102 18-19 0.8616690926433463 1102 20-21 0.8273813292765411 1102 22-23 0.7390819300583686 1102 24-25 0.7639742325575511 1102 26-27 0.7596271461011952 1102 28-29 0.48096157703186293 1102 30-31 0.6818719861927107 1102 32-33 0.684813399695301 1102 34-35 0.6644441656557163 1102 36-37 0.8298074895351917 1102 38-39 0.7616650477877727 1102 40-41 0.9467225685882141 1102 42-43 1.0420512217033107 1102 44-45 0.8803133737612896 1102 46-47 0.872950399526907 1102 48-49 1.0893629206485684 1102 50-51 1.2315737908162845 1102 52-53 1.1186516908643043 1102 54-55 0.9664707703928457 1102 56-57 1.158119419120446 1102 58-59 1.0951624387681314 1102 60-61 0.9407742338144232 1102 62-63 0.9961226783370627 1102 64-65 1.2041852889466327 1102 66-67 1.082366702377616 1102 68-69 1.1008736614624155 1102 70-71 1.224579162488162 1102 72-73 1.2852803961904762 1102 74-75 1.2568225319050335 1102 76-77 1.223200674125433 1102 78-79 1.3317891321500213 1102 80-81 1.4880140616395714 1102 82-83 1.5195665477798386 1102 84-85 1.461355367796898 1102 86-87 1.811544269934192 1102 88-89 1.859995465413352 1102 90-91 1.9705999241901182 1102 92-93 1.816159365881294 1102 94-95 2.01317908695189 1102 96-97 1.9839209916541556 1102 98-99 2.1504053647293873 1102 100-101 1.6902499186705828 1103 1 0.4403778894769026 1103 2 0.41137147574682587 1103 3 0.3233208507384866 1103 4 0.2765960152277529 1103 5 0.2504653847083418 1103 6 0.3955313274099552 1103 7 0.29624433881053136 1103 8 0.3282039240919019 1103 9 0.5267829934206034 1103 10-11 0.48305287640013717 1103 12-13 0.5906114738668364 1103 14-15 0.6935294408899821 1103 16-17 0.6535518116887715 1103 18-19 0.7102836999335054 1103 20-21 0.6138219551301134 1103 22-23 0.7124736100856879 1103 24-25 0.7518030642241413 1103 26-27 0.773886963799356 1103 28-29 0.7721337658647016 1103 30-31 0.8764174620785496 1103 32-33 0.7871321494170189 1103 34-35 0.7694325566614921 1103 36-37 0.9269617390624205 1103 38-39 0.8096616271563803 1103 40-41 0.9199571996360163 1103 42-43 1.065936062317725 1103 44-45 0.8981436636487032 1103 46-47 0.7228183367721215 1103 48-49 0.7540132110998741 1103 50-51 0.923523876032263 1103 52-53 0.9701241175415021 1103 54-55 1.1208147694059036 1103 56-57 1.1011628829039424 1103 58-59 1.0244355392887918 1103 60-61 1.277370384085927 1103 62-63 1.0231779691462286 1103 64-65 1.1860539140944475 1103 66-67 1.2084332735391108 1103 68-69 1.2632628165861135 1103 70-71 1.3201307724698417 1103 72-73 1.4578351238049017 1103 74-75 1.540454137099971 1103 76-77 1.515922940213354 1103 78-79 1.5058383766372643 1103 80-81 1.6579883890431937 1103 82-83 1.5113024033886724 1103 84-85 1.6778008199403978 1103 86-87 1.8267641149035008 1103 88-89 1.7224837744500228 1103 90-91 1.6588878721544695 1103 92-93 1.5980693009069036 1103 94-95 1.7950581480107743 1103 96-97 1.8319150577287822 1103 98-99 1.8456777821954304 1103 100-101 1.6593744210032906 1104 1 0.3857832893342952 1104 2 0.3635128411853934 1104 3 0.3280192664269208 1104 4 0.3958351882534572 1104 5 0.4067581644714622 1104 6 0.40117099599716255 1104 7 0.4075097292614345 1104 8 0.4936091669731866 1104 9 0.6238956594001976 1104 10-11 0.6306776165593959 1104 12-13 0.6001378709089167 1104 14-15 0.7995553914557902 1104 16-17 0.830720421538544 1104 18-19 0.7817067426980273 1104 20-21 0.7880194902429523 1104 22-23 0.777689213752403 1104 24-25 0.8674373412857577 1104 26-27 0.8657891097231385 1104 28-29 0.7817704688109544 1104 30-31 0.7245100514175746 1104 32-33 0.82685942154054 1104 34-35 0.8602540428267602 1104 36-37 1.0803633811532691 1104 38-39 1.077005766483552 1104 40-41 1.043926666575345 1104 42-43 1.1148984200505865 1104 44-45 0.975210722031143 1104 46-47 0.904243836816498 1104 48-49 0.7035216041897883 1104 50-51 0.9386754515591988 1104 52-53 0.974846200397117 1104 54-55 1.0700002143395864 1104 56-57 1.1198819216305438 1104 58-59 1.050821230795723 1104 60-61 1.1335913170915433 1104 62-63 1.1701167237098566 1104 64-65 1.1801349487843567 1104 66-67 1.1897222647442227 1104 68-69 1.3729576006926436 1104 70-71 1.3476477787180947 1104 72-73 1.3975322685906093 1104 74-75 1.3081901129449705 1104 76-77 1.1974772814178358 1104 78-79 1.2729091964087687 1104 80-81 1.4035641683541016 1104 82-83 1.3359844211112772 1104 84-85 1.3922040420561572 1104 86-87 1.6958197768204357 1104 88-89 1.7493079726794498 1104 90-91 1.6593480536550764 1104 92-93 1.6509389147396405 1104 94-95 1.7336186650176266 1104 96-97 1.630980265000641 1104 98-99 2.0326960994960714 1104 100-101 1.4828328703329117 1105 1 0.3420145614169954 1105 2 0.28889406654227656 1105 3 0.239176097786725 1105 4 0.20139757473695852 1105 5 0.22550364296141368 1105 6 0.38368527049303935 1105 7 0.37260318940604265 1105 8 0.34288615091958974 1105 9 0.5071639586428773 1105 10-11 0.5627587055688181 1105 12-13 0.5461820070884329 1105 14-15 0.6588983135584314 1105 16-17 0.599512342142674 1105 18-19 0.4911129588487597 1105 20-21 0.49484679309944113 1105 22-23 0.641150305927944 1105 24-25 0.6376755776996745 1105 26-27 0.6539543993919921 1105 28-29 0.5458709698864723 1105 30-31 0.6250990179968454 1105 32-33 0.5823530668861991 1105 34-35 0.6229364281300533 1105 36-37 0.7290071976566992 1105 38-39 0.7077272959355625 1105 40-41 0.899678084095342 1105 42-43 0.8711993127491624 1105 44-45 0.987867432249935 1105 46-47 0.8440272242140878 1105 48-49 0.7234756557181186 1105 50-51 0.8588144204364596 1105 52-53 0.6453000507803139 1105 54-55 0.8129514248701781 1105 56-57 0.8013757179059908 1105 58-59 0.8959181407638432 1105 60-61 0.9018256486229888 1105 62-63 1.0256205823488003 1105 64-65 1.1432461221839496 1105 66-67 1.2053141619712555 1105 68-69 1.0298602265870862 1105 70-71 1.2247163252359563 1105 72-73 1.3057825101633647 1105 74-75 1.3000985178611977 1105 76-77 1.4235456911910482 1105 78-79 1.5295175659756488 1105 80-81 1.5857195590721496 1105 82-83 1.666144786837961 1105 84-85 1.6164439318887673 1105 86-87 1.8083784961642415 1105 88-89 1.7876554845226131 1105 90-91 1.812642152811403 1105 92-93 1.9895584973677316 1105 94-95 1.9540755510185512 1105 96-97 2.1099906422740204 1105 98-99 2.228570326193733 1105 100-101 1.7324735042606765 1106 1 0.26147285870508696 1106 2 0.1643947010375122 1106 3 0.1824502559055965 1106 4 0.17480143416979388 1106 5 0.31088637446697476 1106 6 0.06118238549932897 1106 7 0.359311385470626 1106 8 0.35092070862040003 1106 9 0.21503362343832322 1106 10-11 0.18491929404267182 1106 12-13 0.08183719831264824 1106 14-15 0.4526935185268357 1106 16-17 0.1913528752351965 1106 18-19 0.04518571686757156 1106 20-21 0.34465655446481236 1106 22-23 0.3218678841448863 1106 24-25 0.20087664601309285 1106 26-27 0.20871903920285462 1106 28-29 0.36911438332242597 1106 30-31 0.022739320157953102 1106 32-33 0.14267920062524553 1106 34-35 -0.26054912799239816 1106 36-37 -0.058568821666511894 1106 38-39 0.23418881995321073 1106 40-41 0.351479422829172 1106 42-43 -0.2289682076249946 1106 44-45 0.026062968037543044 1106 46-47 0.1453960804857175 1106 48-49 0.4811707037749642 1106 50-51 0.24951001476495094 1106 52-53 0.1842811887425384 1106 54-55 0.2865342600013463 1106 56-57 -0.5084717822663372 1106 58-59 -0.20655347185447326 1106 60-61 0.4496801004840769 1106 62-63 0.4599345771820289 1106 64-65 0.511848441810141 1106 66-67 0.1689070089678104 1106 68-69 0.20184674800200142 1106 70-71 0.15054326606965063 1106 72-73 0.4558702335408107 1106 74-75 0.7477900666573056 1106 76-77 0.79614440184044 1106 78-79 0.8858646126072856 1106 80-81 1.0904314681129144 1106 82-83 0.8427327866493108 1106 84-85 0.809718337363492 1106 86-87 0.5371596793541151 1106 88-89 0.6667067198342131 1106 90-91 0.9159651092920562 1106 92-93 0.9076958594572275 1106 94-95 0.7720634222268465 1106 96-97 0.7701508607035272 1106 98-99 0.9882707705657623 1106 100-101 0.5158368885811964 1107 1 0.10914298883654183 1107 2 0.008248657349444244 1107 3 0.01001889652048149 1107 4 0.04997148098210147 1107 5 0.053840891404476565 1107 6 0.2685589981950329 1107 7 0.14206508392675943 1107 8 0.17973427838997225 1107 9 0.1967761251587774 1107 10-11 0.2516743364706642 1107 12-13 0.25282721474558656 1107 14-15 0.3537897687053615 1107 16-17 0.40676051378868294 1107 18-19 0.40550693282778383 1107 20-21 0.4898560811075612 1107 22-23 0.34964016825660593 1107 24-25 0.4114848402922391 1107 26-27 0.38314300611590113 1107 28-29 0.3451472508729765 1107 30-31 0.4155075208477186 1107 32-33 0.3405519451706027 1107 34-35 0.24586430487257616 1107 36-37 0.269941455946622 1107 38-39 0.2951573342990983 1107 40-41 0.36920033533006347 1107 42-43 0.5708409993553403 1107 44-45 0.5629786278484303 1107 46-47 0.353449589454776 1107 48-49 0.3241202163396508 1107 50-51 0.3235541508257995 1107 52-53 0.4700521374493931 1107 54-55 0.5954389929365647 1107 56-57 0.5835942077547926 1107 58-59 0.5873507319127249 1107 60-61 0.46130696149175066 1107 62-63 0.6562527274153922 1107 64-65 0.6354396821308832 1107 66-67 0.6745008843677063 1107 68-69 0.7332514336620903 1107 70-71 0.9499263700324114 1107 72-73 0.9047404833175214 1107 74-75 0.7619386509787383 1107 76-77 0.9328728521387113 1107 78-79 1.145446393305221 1107 80-81 1.115626030083508 1107 82-83 0.8467075019250494 1107 84-85 1.0256390547251826 1107 86-87 1.1954398947699012 1107 88-89 1.3193998507526814 1107 90-91 1.0786185614891686 1107 92-93 1.1734920995747657 1107 94-95 1.3132970513196547 1107 96-97 1.405389857116777 1107 98-99 1.3979172372225896 1107 100-101 1.3439571594358224 1108 1 0.44209622533858095 1108 2 0.29472059656351846 1108 3 0.37452766207286103 1108 4 0.3756098763361493 1108 5 0.3182072779782388 1108 6 0.3646418830790381 1108 7 0.31353071033682767 1108 8 0.4726736575904553 1108 9 0.4154780480291862 1108 10-11 0.580029799830676 1108 12-13 0.5201816818623115 1108 14-15 0.5910856189969493 1108 16-17 0.6486935953690462 1108 18-19 0.7410922774527293 1108 20-21 0.6185560190833499 1108 22-23 0.44464524637025704 1108 24-25 0.5962908165931822 1108 26-27 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-2.5756736756792797 2316 84-85 -2.6136463503641494 2316 86-87 -2.622989294341803 2316 88-89 -2.9776208786344895 2316 90-91 -2.8636167620990385 2316 92-93 -2.746642386152068 2316 94-95 -3.1757129814440077 2316 96-97 -3.3035637085308327 2316 98-99 -3.3674980778926233 2316 100-101 -2.80778892322342 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9703.0 3 3501.0 4 3923.0 5 4640.0 6 5436.0 7 6977.0 8 8663.0 9 11211.0 10 14245.0 11 17985.0 12 22397.0 13 26182.0 14 30126.0 15 34035.0 16 39893.0 17 45747.0 18 43699.0 19 41049.0 20 43409.0 21 46881.0 22 51504.0 23 57407.0 24 63460.0 25 70879.0 26 79550.0 27 88905.0 28 101016.0 29 113598.0 30 130866.0 31 150753.0 32 175256.0 33 206771.0 34 246895.0 35 293186.0 36 325816.0 37 291837.0 38 117352.0 39 4844.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.62821593693202 18.005186563789255 13.863230788224335 23.50336671105439 2 15.896914669840529 19.855134143496567 39.196156736120244 25.05179445054266 3 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22.791709224685874 26.545210976634742 27.787255900736763 22.875823897942624 48-49 22.83327520706349 26.33694409430221 27.7586636888851 23.071117009749205 50-51 22.93964294291097 26.643674916793138 27.54513967116681 22.87154246912908 52-53 23.137982821531793 26.467513865756143 27.507055636826205 22.887447675885856 54-55 22.58905347062312 26.60049322252305 27.61899228574357 23.19146102111026 56-57 22.66248313072598 26.83795857355268 27.547722548970416 22.95183574675093 58-59 22.71529841722866 26.259560633834845 27.613297689271043 23.411843259665453 60-61 22.43844959438521 26.54195853950283 27.948907492887585 23.070684373224374 62-63 22.476490156186504 26.596287611871272 27.890133672777832 23.03708855916439 64-65 22.240966707055062 26.47907437126063 27.776801215976242 23.503157705708066 66-67 22.17665777073248 26.656821664111334 27.775533941738427 23.39098662341776 68-69 22.51698631109233 26.221599044656692 27.96551322881446 23.295901415436518 70-71 22.403011998765802 26.39299247380011 27.551497984357244 23.65249754307684 72-73 22.42257697977048 26.521671446115107 27.4453643091998 23.61038726491461 74-75 22.275860133487633 26.892145096401315 27.306194479891836 23.525800290219216 76-77 22.43011003320013 26.54087088927216 27.36035873041866 23.668660347109054 78-79 22.50448472569026 26.59953565821117 27.35401266797813 23.541966948120443 80-81 22.524778008421 26.85536535421193 27.110081514782962 23.509775122584113 82-83 22.53085864498418 26.726383297652667 27.120980478596802 23.621777578766352 84-85 22.523923484460877 26.569987951608375 27.053012923137864 23.853075640792884 86-87 22.697116379732954 26.459215644459466 26.97423390167677 23.869434074130805 88-89 22.560243738709303 26.489579456950313 26.888788435421095 24.061388368919285 90-91 22.4237560431117 26.51236077709096 27.001637581530314 24.062245598267026 92-93 22.587181501651934 26.004808294712838 27.1746076607496 24.233402542885628 94-95 22.369234676094045 25.921177966525676 27.283471724057996 24.426115633322286 96-97 22.63140984258326 25.66704234114435 27.14929444634685 24.55225336992555 98-99 22.51253154035804 25.806083647051736 26.779486984778917 24.90189782781131 100-101 22.901660517214612 25.039638365974426 27.156723492668554 24.901977624142408 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1294.0 1 879.0 2 664.0 3 894.0 4 777.0 5 803.0 6 1469.5 7 2787.5 8 4301.5 9 4887.5 10 4080.5 11 3318.0 12 3222.5 13 3422.5 14 3733.0 15 3882.5 16 4131.5 17 4321.5 18 4679.5 19 5208.0 20 5783.5 21 6580.0 22 7839.0 23 9525.5 24 11205.5 25 13379.5 26 16199.0 27 19482.5 28 22456.0 29 25815.5 30 30369.0 31 36927.0 32 45750.5 33 49403.0 34 49303.0 35 52203.5 36 56945.5 37 61922.5 38 66576.5 39 69897.5 40 73512.0 41 81260.0 42 88381.5 43 93146.5 44 98229.0 45 105054.5 46 109175.0 47 110797.0 48 112667.5 49 111282.0 50 109728.5 51 108544.5 52 108815.0 53 111595.0 54 113869.0 55 108992.5 56 99197.5 57 91693.0 58 85616.5 59 75855.0 60 62611.0 61 52516.5 62 45016.0 63 36184.5 64 27927.5 65 22211.5 66 17128.0 67 13463.0 68 10362.5 69 7218.0 70 5340.5 71 4090.5 72 3215.0 73 2597.5 74 2000.5 75 1598.5 76 1176.5 77 710.5 78 412.0 79 282.5 80 175.0 81 96.5 82 58.0 83 32.0 84 22.0 85 15.5 86 11.5 87 6.0 88 4.5 89 5.5 90 6.5 91 5.0 92 2.0 93 1.0 94 1.5 95 1.5 96 1.5 97 1.5 98 0.5 99 0.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0076247764966759606 2 0.10598769407284203 3 0.11506480894983723 4 0.1183325703055555 5 0.15913007571634116 6 0.0955242561964512 7 0.02574599856020454 8 0.02756142153560358 9 0.021554021871555855 10-11 0.05065030101363317 12-13 0.05203662401302879 14-15 0.025927540857744444 16-17 0.030119517546393134 18-19 0.03625894797228806 20-21 0.057581916010611314 22-23 0.030631136748551047 24-25 0.0473990435031458 26-27 0.05598104302321398 28-29 0.05259775475087941 30-31 0.06736869623253522 32-33 0.04857081651453972 34-35 0.0616748696278746 36-37 0.05226767784626141 38-39 0.031175763641170758 40-41 0.03607740567474816 42-43 0.03178640591471407 44-45 0.007542257270521459 46-47 0.013170068494258478 48-49 0.00899459565084069 50-51 0.008235418770219273 52-53 0.009291664864996895 54-55 0.011074080149934133 56-57 0.013896237684418094 58-59 0.0189629181703045 60-61 0.02435967556080891 62-63 0.011024568614241432 64-65 0.008631511055760882 66-67 0.010248887888389116 68-69 0.010545957102545323 70-71 0.030053502165469533 72-73 0.02741288692852548 74-75 0.028122552273454192 76-77 0.02287432949002788 78-79 0.004868634343115603 80-81 0.004835626652653802 82-83 0.005891872747431424 84-85 0.004852130497884702 86-87 0.012674953137331465 88-89 0.03657252103167517 90-91 0.03450954037781263 92-93 0.028799209927921107 94-95 0.04606223203944287 96-97 0.04178773612463968 98-99 0.024409187096501615 100-101 0.07350812665843015 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3029597.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.541548016367436 #Duplication Level Percentage of deduplicated Percentage of total 1 72.59489341843056 33.060838243585316 2 13.680172971538674 12.46032508511081 3 5.235872201686194 7.153491758419659 4 2.604114180442682 4.74381563954935 5 1.5059015526096415 3.429054393304713 6 0.9276970877484806 2.5349256879805 7 0.6394283583429584 2.0384390099151997 8 0.4633977234535538 1.6883079738668305 9 0.3523763877193188 1.4442989563038122 >10 1.7718899234844734 14.70116889186146 >50 0.1312610948478294 4.155461188768006 >100 0.08415270591008105 7.217904513796261 >500 0.005115434421484786 1.6722879124918104 >1k 0.0035077264603706197 2.7011603215929454 >5k 1.4615526918210916E-4 0.5618778225232396 >10k+ 7.307763459105458E-5 0.43664260093021995 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13120 0.4330608988588251 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9931 0.32779937397614267 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6952 0.22946946409043842 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3516 0.11605503966369124 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3471 0.11456969359291021 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3053 0.10077247897987753 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.601538092360139E-5 2 3.3007690461800694E-5 0.0 0.0 0.0 6.601538092360139E-5 3 3.3007690461800694E-5 0.0 0.0 0.0 6.601538092360139E-5 4 3.3007690461800694E-5 0.0 0.0 0.0 6.601538092360139E-5 5 3.3007690461800694E-5 0.0 0.0 0.0 9.902307138540208E-5 6 3.3007690461800694E-5 0.0 0.0 0.0 9.902307138540208E-5 7 1.3203076184720278E-4 0.0 0.0 0.0 1.3203076184720278E-4 8 1.3203076184720278E-4 0.0 0.0 0.0 1.3203076184720278E-4 9 1.3203076184720278E-4 0.0 0.0 0.0 1.3203076184720278E-4 10-11 1.3203076184720278E-4 0.0 0.0 1.6503845230900347E-5 1.6503845230900348E-4 12-13 1.3203076184720278E-4 0.0 0.0 3.3007690461800694E-5 3.4658074984890734E-4 14-15 1.3203076184720278E-4 0.0 0.0 3.3007690461800694E-5 4.125961307725087E-4 16-17 1.4853460707810314E-4 0.0 0.0 3.3007690461800694E-5 4.6210766646520975E-4 18-19 1.6503845230900348E-4 0.0 0.0 3.3007690461800694E-5 4.7861151169611006E-4 20-21 1.6503845230900348E-4 0.0 0.0 4.951153569270104E-5 5.611307378506118E-4 22-23 1.9804614277080417E-4 0.0 0.0 3.135730593871066E-4 5.611307378506118E-4 24-25 2.6406152369440555E-4 0.0 0.0 6.271461187742132E-4 5.611307378506118E-4 26-27 2.6406152369440555E-4 0.0 0.0 0.0013863229993956293 6.601538092360138E-4 28-29 3.4658074984890734E-4 0.0 0.0 0.005066680485886406 6.931614996978146E-4 30-31 3.6308459507980765E-4 0.0 0.0 0.01619027217151324 6.931614996978146E-4 32-33 3.7958844031070796E-4 0.0 0.0 0.03919663242338832 7.921845710832167E-4 34-35 4.456038212343093E-4 0.0 0.0 0.07567013038367809 7.921845710832167E-4 36-37 5.281230473888111E-4 0.0 0.0 0.1367508615832403 7.921845710832167E-4 38-39 5.281230473888111E-4 0.0 0.0 0.22636674118702915 8.58199952006818E-4 40-41 5.281230473888111E-4 0.0 0.0 0.3233928472994923 8.747037972377184E-4 42-43 5.776345830815122E-4 0.0 0.0 0.42180527641135107 8.912076424686188E-4 44-45 5.941384283124125E-4 0.0 0.0 0.5498256038674451 9.407191781613197E-4 46-47 6.931614996978147E-4 0.0 0.0 0.700901803111107 9.902307138540207E-4 48-49 7.59176880621416E-4 0.0 0.0 0.8573417520548112 9.902307138540207E-4 50-51 7.59176880621416E-4 0.0 0.0 1.0360123805245385 0.0010562460947776222 52-53 7.59176880621416E-4 0.0 0.0 1.205143786450805 0.0010892537852394228 54-55 7.59176880621416E-4 0.0 0.0 1.3928585221070657 0.001188276856624825 56-57 7.921845710832167E-4 0.0 0.0 1.6117985329401896 0.0012872999280102273 58-59 7.921845710832167E-4 0.0 0.0 1.846417196742669 0.0013533153089338285 60-61 7.921845710832167E-4 0.0 0.0 2.10361312082102 0.0013533153089338285 62-63 8.08688416314117E-4 0.0 0.0 2.3566005643654915 0.0014523383803192306 64-65 8.251922615450174E-4 0.0 0.0 2.6140770538127676 0.0014688422255501308 66-67 8.58199952006818E-4 0.0 0.0 2.922121325047523 0.0015018499160119317 68-69 8.58199952006818E-4 0.0 0.0 3.2578425447344976 0.001518353761242832 70-71 8.58199952006818E-4 0.0 0.0 3.6133848825437838 0.0015348576064737325 72-73 8.58199952006818E-4 0.0 0.0 3.978895542872534 0.0016338806778591344 74-75 8.58199952006818E-4 0.0 0.0 4.350232060567791 0.001716399904013636 76-77 8.58199952006818E-4 0.0 0.0 4.750879407393128 0.0017494075944754369 78-79 8.58199952006818E-4 0.0 0.0 5.185359636941811 0.001765911439706337 80-81 9.572230233922201E-4 0.0 0.0 5.6526990223452165 0.0017824152849372375 82-83 9.737268686231204E-4 1.6503845230900347E-5 0.0 6.127399122721603 0.0018814383563226396 84-85 0.0010232384043158214 3.3007690461800694E-5 0.0 6.611275361046371 0.00189794220155354 86-87 0.0010562460947776222 3.3007690461800694E-5 0.0 7.118933640348866 0.0019309498920153407 88-89 0.0010562460947776222 3.3007690461800694E-5 0.0 7.652469948973411 0.001947453737246241 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6515 0.0 49.921944 1 GTATCAA 10900 0.0 44.627132 1 TATCAAC 14330 0.0 33.54653 2 ATCAACG 14385 0.0 33.41827 3 TCAACGC 14545 0.0 33.050655 4 CAACGCA 14810 0.0 32.427162 5 AACGCAG 15375 0.0 31.274635 6 GTACATG 13845 0.0 30.051414 1 TACATGG 13980 0.0 29.318567 2 GTGGTAT 2600 0.0 27.432648 1 ACATGGG 14675 0.0 26.89321 3 ACGCAGA 17845 0.0 26.629787 7 CGCAGAG 18035 0.0 26.296543 8 TGGTATC 2705 0.0 24.433752 2 GCAGAGT 19380 0.0 23.710997 9 CATGGGA 7515 0.0 23.347479 4 CATGGGG 7300 0.0 23.058073 4 GAGTACT 11305 0.0 21.331835 12-13 AGGTAAG 1250 0.0 20.92162 2 AGTACTT 11715 0.0 20.909763 12-13 >>END_MODULE