FastQCFastQC Report
Thu 26 May 2016
SRR938495_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938495_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3029597
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132820.43840814471363687No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105850.34938640353816036No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78790.2600675931485277No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA37510.1238118469222144No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA34490.1138435244027506No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65750.051.6840361
GTATCAA109900.045.322071
TATCAAC148450.033.5206682
ATCAACG150600.032.8065723
TCAACGC153050.032.281414
CAACGCA155200.031.834215
AACGCAG160100.030.8752986
ACGCAGA183750.026.7463637
GTGGTAT25350.026.4354861
CGCAGAG185200.026.383128
GTACATG133700.026.2699451
TACATGG135950.025.2758182
TGGTATC25750.024.548252
ACATGGG139900.023.7022843
GCAGAGT202300.023.6600919
GTGTAAG9450.022.1292651
GAGTACT118450.021.76789912-13
AGTACTT123350.021.69234512-13
AGAGTAC188300.021.30875810-11
GTATAGG6250.021.2923771
Produced by FastQC (version 0.11.5)