##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938495_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3029597 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.809560479496117 31.0 31.0 34.0 30.0 34.0 2 32.03312651814746 31.0 31.0 34.0 30.0 34.0 3 32.09118011405477 33.0 31.0 34.0 30.0 34.0 4 35.77082958558515 37.0 35.0 37.0 35.0 37.0 5 35.66972768985446 37.0 35.0 37.0 33.0 37.0 6 35.670696135492605 37.0 35.0 37.0 33.0 37.0 7 35.60363342055065 37.0 35.0 37.0 33.0 37.0 8 35.583153468926724 37.0 35.0 37.0 33.0 37.0 9 37.16508169238351 39.0 37.0 39.0 33.0 39.0 10-11 37.06668213627093 39.0 37.0 39.0 33.0 39.0 12-13 36.9595447843393 39.0 37.0 39.0 33.0 39.0 14-15 38.00135975180857 40.0 37.0 41.0 33.0 41.0 16-17 37.931068719701 40.0 37.0 41.0 33.0 41.0 18-19 38.167899558918236 40.0 37.5 41.0 33.0 41.0 20-21 38.14259223256427 40.0 37.0 41.0 33.0 41.0 22-23 38.034626057525145 40.0 37.0 41.0 33.0 41.0 24-25 37.913594613408975 40.0 37.0 41.0 33.0 41.0 26-27 37.725025308646664 40.0 37.0 41.0 32.0 41.0 28-29 37.606366622359346 39.5 37.0 41.0 32.0 41.0 30-31 37.344691554685326 39.0 36.0 41.0 31.5 41.0 32-33 37.13481809626825 39.0 36.0 41.0 31.0 41.0 34-35 37.018853002561066 39.0 36.0 41.0 31.0 41.0 36-37 36.960657473584774 39.0 35.5 41.0 31.0 41.0 38-39 36.79286485958363 39.0 35.0 41.0 30.0 41.0 40-41 36.616444035295785 39.0 35.0 41.0 30.0 41.0 42-43 36.38846222781446 39.0 35.0 40.0 30.0 41.0 44-45 36.07101208510571 39.0 35.0 40.0 29.5 41.0 46-47 35.652496685202685 38.0 35.0 40.0 28.5 41.0 48-49 35.26727927839907 38.0 34.0 40.0 27.0 41.0 50-51 34.89010469049184 38.0 34.0 40.0 26.0 41.0 52-53 34.40040375006973 38.0 33.0 40.0 24.0 41.0 54-55 34.06507614709152 37.0 33.0 40.0 24.0 41.0 56-57 33.734978117551606 37.0 33.0 40.0 23.0 41.0 58-59 33.27136447520908 36.5 32.0 39.0 22.0 41.0 60-61 32.88531048849072 36.0 31.5 39.0 21.5 40.0 62-63 32.41717974370849 35.5 31.0 39.0 19.5 40.0 64-65 31.905029282772595 35.0 31.0 38.0 18.0 40.0 66-67 31.419261868822815 35.0 30.0 38.0 16.0 40.0 68-69 30.95336095856974 34.5 29.5 37.5 13.0 39.5 70-71 30.41091620436646 34.0 29.0 37.0 8.0 39.0 72-73 29.94322380171356 34.0 29.0 36.0 7.5 39.0 74-75 29.375331108394946 34.0 28.0 36.0 7.0 38.5 76-77 28.14371911511663 31.5 26.5 34.5 2.0 36.5 78-79 28.841487993287558 33.0 28.0 35.0 2.0 37.0 80-81 28.498051060916683 33.0 27.5 35.0 2.0 36.5 82-83 28.087435721648788 33.0 26.5 35.0 2.0 36.0 84-85 27.563080832203095 33.0 26.0 35.0 2.0 36.0 86-87 26.97859220219719 32.5 25.5 35.0 2.0 35.0 88-89 26.261314788732626 32.0 24.5 34.0 2.0 35.0 90-91 25.69727673350614 31.5 23.0 34.0 2.0 35.0 92-93 24.913354812537776 31.0 19.5 34.0 2.0 35.0 94-95 24.139022945956178 31.0 14.0 34.0 2.0 35.0 96-97 23.115742951950374 30.5 2.0 34.0 2.0 35.0 98-99 21.806835529610044 30.0 2.0 34.0 2.0 35.0 100-101 17.900225013425878 23.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2857839276330374 1101 2 0.2899656696542934 1101 3 0.3075848492502047 1101 4 0.23202308702718 1101 5 0.11220188797627628 1101 6 0.22521332074063594 1101 7 0.28086789227777587 1101 8 0.21810184356909446 1101 9 0.29383601657281133 1101 10-11 0.29886721761167223 1101 12-13 0.43009640964066165 1101 14-15 0.462952867659304 1101 16-17 0.3952105762413325 1101 18-19 0.4095048451835339 1101 20-21 0.33201462580348107 1101 22-23 0.43252427472950217 1101 24-25 0.5318662879291836 1101 26-27 0.4500281224561178 1101 28-29 0.3471853107255427 1101 30-31 0.5065463888728843 1101 32-33 0.48226063093067495 1101 34-35 0.5050925033962912 1101 36-37 0.5755009973804945 1101 38-39 0.6492413827066841 1101 40-41 0.6039767286744535 1101 42-43 0.6122745449648264 1101 44-45 0.7043340181445856 1101 46-47 0.5890846348014875 1101 48-49 0.8093131303012271 1101 50-51 0.8315077635808308 1101 52-53 0.9146889580221256 1101 54-55 0.7744566745991222 1101 56-57 0.7431814390715772 1101 58-59 0.8245799524271789 1101 60-61 0.7686494267363528 1101 62-63 0.7962796300992991 1101 64-65 0.7811822700129021 1101 66-67 0.579808939690519 1101 68-69 0.7641178504639292 1101 70-71 0.7308323335715627 1101 72-73 0.7145844853131216 1101 74-75 0.7057518196860677 1101 76-77 0.6436401334625224 1101 78-79 0.6532804413461974 1101 80-81 0.8112980568330883 1101 82-83 0.8777473367208906 1101 84-85 1.037672546567773 1101 86-87 1.1475904108153046 1101 88-89 0.9769918618568383 1101 90-91 1.1691170394985626 1101 92-93 1.4433573131234887 1101 94-95 1.3742065187128674 1101 96-97 1.2963075879360773 1101 98-99 1.4944029039776048 1101 100-101 1.8186657393483827 1102 1 0.31126531726293294 1102 2 0.29814964639830777 1102 3 0.26104355851070693 1102 4 0.19258581948448494 1102 5 0.04157211905002356 1102 6 0.1190183817284236 1102 7 0.21338532048204684 1102 8 0.1976986564284715 1102 9 0.08134796095318109 1102 10-11 0.29430370780298887 1102 12-13 0.26858525471573813 1102 14-15 0.341988723327475 1102 16-17 0.3975231293227992 1102 18-19 0.27663424661625413 1102 20-21 0.30720353361808606 1102 22-23 0.33101146557935834 1102 24-25 0.4838349840701923 1102 26-27 0.4045304057576615 1102 28-29 0.2746895622861203 1102 30-31 0.3108243767163472 1102 32-33 0.274801285533151 1102 34-35 0.31883131468079995 1102 36-37 0.43795922987444413 1102 38-39 0.4868833451557677 1102 40-41 0.40907967729731354 1102 42-43 0.41905559077139287 1102 44-45 0.3944144498032287 1102 46-47 0.32284320264590605 1102 48-49 0.3342819118433056 1102 50-51 0.39991401171058527 1102 52-53 0.33603693640422705 1102 54-55 0.3742486860361396 1102 56-57 0.549478590374683 1102 58-59 0.5273917318557508 1102 60-61 0.5437948270824009 1102 62-63 0.4049001609173146 1102 64-65 0.4603763529971836 1102 66-67 0.4828257489052241 1102 68-69 0.4902932077530444 1102 70-71 0.5187447887444137 1102 72-73 0.6463862929912843 1102 74-75 0.607807429896809 1102 76-77 0.5888472175812218 1102 78-79 0.7421213472429713 1102 80-81 0.7038652059372872 1102 82-83 0.7980814633202264 1102 84-85 0.9115225406165983 1102 86-87 0.7425024578114403 1102 88-89 0.7162302189018561 1102 90-91 0.7478237984880352 1102 92-93 1.088815153579521 1102 94-95 1.0735932449675083 1102 96-97 1.0087474289421685 1102 98-99 1.2270171648785109 1102 100-101 1.4941850887402524 1103 1 0.1850175848090707 1103 2 0.14534570973791006 1103 3 0.24484569771937714 1103 4 0.10641874718238142 1103 5 0.04742133238262625 1103 6 0.03714789393632856 1103 7 0.15082496793706923 1103 8 0.08353935773190102 1103 9 0.1914008944967165 1103 10-11 0.18830840447328256 1103 12-13 0.2795499781468678 1103 14-15 0.40319700025521854 1103 16-17 0.2598353164129108 1103 18-19 0.33733539950375757 1103 20-21 0.29848629462844656 1103 22-23 0.26272941428749164 1103 24-25 0.3291775249900013 1103 26-27 0.3865155099617681 1103 28-29 0.2996266693367531 1103 30-31 0.16168780802094318 1103 32-33 0.14685095587700658 1103 34-35 0.28584413975209344 1103 36-37 0.31658716017430066 1103 38-39 0.3399087833906762 1103 40-41 0.37347217012645473 1103 42-43 0.42620285027945926 1103 44-45 0.3625037812019656 1103 46-47 0.2905279192286301 1103 48-49 0.2942258743179096 1103 50-51 0.42245832255070326 1103 52-53 0.3885255503532079 1103 54-55 0.4874847638227209 1103 56-57 0.4238879503407702 1103 58-59 0.5326901255547014 1103 60-61 0.4724202121936756 1103 62-63 0.4708378319919362 1103 64-65 0.3899626584282281 1103 66-67 0.32694800266907365 1103 68-69 0.49908463356768706 1103 70-71 0.40667362909908533 1103 72-73 0.43255798363056286 1103 74-75 0.37056300287106225 1103 76-77 0.35570590245995604 1103 78-79 0.4428591585007169 1103 80-81 0.4749813087649457 1103 82-83 0.7045549686725856 1103 84-85 0.708337648549815 1103 86-87 0.6479748556559279 1103 88-89 0.5196075757050167 1103 90-91 0.589178111829959 1103 92-93 0.91036636883371 1103 94-95 0.7937208003783027 1103 96-97 0.8838688676077844 1103 98-99 1.270875101765231 1103 100-101 1.5111356619435128 1104 1 0.21682323872625986 1104 2 0.24103508541373486 1104 3 0.2342484821132942 1104 4 0.16863526191428235 1104 5 0.10710509797382883 1104 6 0.10022294723273717 1104 7 0.12732709345395676 1104 8 0.20274846379522415 1104 9 0.2715375603120904 1104 10-11 0.2686680367386387 1104 12-13 0.30094739154285577 1104 14-15 0.4048438607632363 1104 16-17 0.4122757213388226 1104 18-19 0.34941803172745267 1104 20-21 0.3197942316423905 1104 22-23 0.3411636138270637 1104 24-25 0.3869368238622499 1104 26-27 0.23001201996023468 1104 28-29 0.2755222029438329 1104 30-31 0.31581232509299895 1104 32-33 0.4710273897782926 1104 34-35 0.3562459321777496 1104 36-37 0.4423368569387165 1104 38-39 0.3511491784150209 1104 40-41 0.37806221192435885 1104 42-43 0.6071392489939456 1104 44-45 0.5234539817389461 1104 46-47 0.46692970078671436 1104 48-49 0.4440770187039291 1104 50-51 0.6160983751256239 1104 52-53 0.6802646222500783 1104 54-55 0.578293269271299 1104 56-57 0.46747520460523617 1104 58-59 0.8158250954243513 1104 60-61 0.7236009442215661 1104 62-63 0.6237693910122388 1104 64-65 0.69819140642905 1104 66-67 0.7066160947995606 1104 68-69 0.74818688116369 1104 70-71 0.8567923573752516 1104 72-73 0.6655978923912222 1104 74-75 0.871863066929393 1104 76-77 0.9090442711038556 1104 78-79 1.0405909744788318 1104 80-81 0.849100416198791 1104 82-83 0.9289287698430257 1104 84-85 0.8995586389120902 1104 86-87 1.084027558573208 1104 88-89 1.016726520728966 1104 90-91 1.0977374756661682 1104 92-93 1.2726883594356693 1104 94-95 1.0821597514639443 1104 96-97 1.1678166718440828 1104 98-99 1.442549366679323 1104 100-101 1.489317190323284 1105 1 0.11300148112061947 1105 2 0.1468033832379092 1105 3 0.12288817925510642 1105 4 0.031845107075085366 1105 5 -0.01703929756659761 1105 6 -0.0031056217884142256 1105 7 0.05788736635315672 1105 8 0.015323637889324004 1105 9 0.11551827080550936 1105 10-11 0.057541561556085696 1105 12-13 0.12464733860394261 1105 14-15 0.19364906999673082 1105 16-17 0.1406866634073225 1105 18-19 0.14279176052706788 1105 20-21 0.16439942827949494 1105 22-23 0.058639579962061816 1105 24-25 0.059229293998292576 1105 26-27 0.022259506213430313 1105 28-29 0.0676969478526459 1105 30-31 0.18716670424108628 1105 32-33 0.2395963201457647 1105 34-35 0.16256523717449767 1105 36-37 0.1504643120300173 1105 38-39 0.06605502603608926 1105 40-41 0.05168763031475265 1105 42-43 0.09733760817163528 1105 44-45 0.2604747867847621 1105 46-47 0.12512232236320386 1105 48-49 0.16194063605949793 1105 50-51 0.3100492117361924 1105 52-53 0.16681689246658493 1105 54-55 0.18681081542545286 1105 56-57 0.1347637851787553 1105 58-59 0.28413443792089765 1105 60-61 0.2822605970036349 1105 62-63 0.23258238504484297 1105 64-65 0.19950659490119094 1105 66-67 0.1045508534168853 1105 68-69 0.06765449622920627 1105 70-71 0.34259502860536983 1105 72-73 0.3188471665381378 1105 74-75 0.3140312015678326 1105 76-77 0.22802981872645844 1105 78-79 0.527519377590707 1105 80-81 0.6031357734853238 1105 82-83 0.491387597504481 1105 84-85 0.49934296820227786 1105 86-87 0.4047586147582365 1105 88-89 0.5535881118806216 1105 90-91 0.7701644208304259 1105 92-93 0.7459766256667208 1105 94-95 0.8296906027022182 1105 96-97 0.819979535288379 1105 98-99 0.8138534737282548 1105 100-101 1.0808875919723917 1106 1 -0.03254654785453681 1106 2 0.07723922314348641 1106 3 0.08406826233808573 1106 4 0.02233169120844991 1106 5 -0.03953251919183032 1106 6 -0.08669178028282687 1106 7 0.026111814191686733 1106 8 0.1109035867625181 1106 9 -0.13502286133891062 1106 10-11 0.062360658131176194 1106 12-13 0.03310346762563654 1106 14-15 -0.00755946670433616 1106 16-17 0.16092086652077597 1106 18-19 0.1850674585185459 1106 20-21 0.22672375753013796 1106 22-23 0.23612811996559913 1106 24-25 0.2849987273456236 1106 26-27 0.14261258305083402 1106 28-29 0.24724579716973238 1106 30-31 0.19706302696109645 1106 32-33 0.15307410269842592 1106 34-35 0.049922022260851406 1106 36-37 0.21856654409224063 1106 38-39 0.0852926599201922 1106 40-41 0.24683781764009183 1106 42-43 0.22350541635806564 1106 44-45 0.049463317938460705 1106 46-47 -0.5172986889440381 1106 48-49 -0.18049814089445704 1106 50-51 0.10657896309299275 1106 52-53 0.017415718988445406 1106 54-55 0.18587042345767912 1106 56-57 0.09693884342006953 1106 58-59 -0.12903255575020012 1106 60-61 0.02080760896148348 1106 62-63 -0.13844852926922613 1106 64-65 -0.1513872446959077 1106 66-67 -0.3361570152584896 1106 68-69 -0.4094988381221363 1106 70-71 -0.2770448388690596 1106 72-73 0.14922915982391416 1106 74-75 0.13583775236422824 1106 76-77 0.10177340217832764 1106 78-79 0.2829704683585419 1106 80-81 -0.20115869782562967 1106 82-83 0.04773416041800971 1106 84-85 0.24010156131696903 1106 86-87 0.40526932213330014 1106 88-89 0.5088878868307738 1106 90-91 0.5023605988637563 1106 92-93 0.514824729057505 1106 94-95 0.7605470025180985 1106 96-97 0.749703430094204 1106 98-99 0.8032928081673241 1106 100-101 0.7446740471399131 1107 1 0.036537095065597214 1107 2 0.042036960289379977 1107 3 0.07415782807449744 1107 4 0.0028085776402164697 1107 5 -0.006400854769815112 1107 6 0.06319649892230927 1107 7 0.07676724598913864 1107 8 0.0879241127356849 1107 9 0.20432157000082896 1107 10-11 0.1332790984510055 1107 12-13 0.1124088293357488 1107 14-15 0.12933786811714754 1107 16-17 0.18072830995595268 1107 18-19 0.20436725586756666 1107 20-21 0.236336436834776 1107 22-23 0.1813557818400824 1107 24-25 0.17972699146869076 1107 26-27 0.19449868310758234 1107 28-29 0.1954314194916975 1107 30-31 0.20829820960346268 1107 32-33 0.38143324616292773 1107 34-35 0.18205736724758026 1107 36-37 0.21218570755960542 1107 38-39 0.2715610579117964 1107 40-41 0.2380914485528578 1107 42-43 0.09464412221471008 1107 44-45 0.13734402246640087 1107 46-47 0.2496806831147822 1107 48-49 0.28579944544116387 1107 50-51 0.45551632788220076 1107 52-53 0.4126875687435927 1107 54-55 0.32998418925449613 1107 56-57 0.3854219219012762 1107 58-59 0.2357372237721762 1107 60-61 0.419335696500184 1107 62-63 0.5387812296359797 1107 64-65 0.3832909171927348 1107 66-67 0.49883873102461607 1107 68-69 0.42920280817025613 1107 70-71 0.5778405730080607 1107 72-73 0.6338454147999464 1107 74-75 0.5581612147550068 1107 76-77 0.6553679888469084 1107 78-79 0.8106722837966487 1107 80-81 0.8652565382851698 1107 82-83 0.8606246830239321 1107 84-85 0.9217633244296053 1107 86-87 1.1969218715582741 1107 88-89 1.1744950682206934 1107 90-91 1.2079159959649175 1107 92-93 1.24976467501655 1107 94-95 1.2366243765993552 1107 96-97 1.3220859367709679 1107 98-99 1.2608551520483893 1107 100-101 1.2620371835506532 1108 1 0.2068845075651602 1108 2 0.18914532244964022 1108 3 0.19747922107293903 1108 4 0.15998546012096426 1108 5 0.09400934013644502 1108 6 0.16561136747124294 1108 7 0.10455237430353748 1108 8 0.1083735220472235 1108 9 0.27074331645437155 1108 10-11 0.2703727831796101 1108 12-13 0.28966806046928895 1108 14-15 0.38076887524788106 1108 16-17 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2316 68-69 -0.8527031791997324 2316 70-71 -0.7691410202271705 2316 72-73 -0.7653235983800322 2316 74-75 -0.5098681416167352 2316 76-77 -0.6947109221249086 2316 78-79 -0.8368563285269488 2316 80-81 -0.7050995652159351 2316 82-83 -0.8144993516117367 2316 84-85 -1.012842578423328 2316 86-87 -1.133094705422856 2316 88-89 -1.2955824962262241 2316 90-91 -1.1395803015351227 2316 92-93 -1.2309706162834466 2316 94-95 -1.345995437505696 2316 96-97 -1.5300068183928381 2316 98-99 -1.187479982565069 2316 100-101 -1.7455539614603204 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 17.0 4 52.0 5 119.0 6 221.0 7 655.0 8 1467.0 9 2511.0 10 4057.0 11 6418.0 12 9019.0 13 12742.0 14 16631.0 15 21765.0 16 27513.0 17 34921.0 18 32675.0 19 26496.0 20 26086.0 21 27920.0 22 30044.0 23 33870.0 24 39463.0 25 46365.0 26 55315.0 27 66062.0 28 79032.0 29 94908.0 30 112124.0 31 133831.0 32 161133.0 33 198641.0 34 251802.0 35 331748.0 36 429842.0 37 475019.0 38 229521.0 39 9588.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.22235553714452 18.873608101439636 14.553874540572389 23.35016182084345 2 16.377754763165996 20.598389446861127 38.398059727320884 24.625796062651997 3 19.268965476266313 24.017880926076966 29.98943423828318 26.723719359373536 4 13.425354403689468 16.605646429424915 35.44697048715406 34.522028679731555 5 14.054982783825812 37.29564564698439 33.50108659866055 15.148284970529252 6 33.33784438585211 35.38943806368902 16.611983908085435 14.660733642373438 7 29.379155049334944 32.84978167063144 19.673672769018456 18.09739051101516 8 27.46309162571788 34.43207132829878 19.11561174638079 18.989225299602555 9 27.15324843535295 16.064578886234703 19.378584016289953 37.40358866212239 10-11 25.680065698507093 26.2514453242461 26.608720565804628 21.45976841144218 12-13 26.403033142691918 23.45816951891621 27.37974720730183 22.759050131090042 14-15 22.86376702908011 25.00563606314635 25.68085788307818 26.44973902469536 16-17 22.524266428835254 28.346955057058743 26.380010278594813 22.74876823551119 18-19 22.56869478019684 26.219724933712307 29.12625012501663 22.08533016107423 20-21 23.14028895592384 25.483752459485537 29.200616451627067 22.17534213296356 22-23 22.85485495265542 25.479576986642115 29.189542371477128 22.47602568922533 24-25 22.700945153375955 25.807696402178316 29.249992366481507 22.24136607796422 26-27 22.226232744644754 26.209424199558313 29.02898650467457 22.53535655112236 28-29 22.3105875937791 26.423054185826285 29.06494370695998 22.201414513434642 30-31 22.399456683192827 25.92174059428164 28.964941872363458 22.71386085016207 32-33 22.16091018421833 26.042531574874378 28.955571300288867 22.840986940618425 34-35 22.871327069680905 26.20624166659239 28.66723578950774 22.255195474218965 36-37 23.156450919579015 25.752438355223674 28.578873552850787 22.512237172346534 38-39 22.48705992138163 26.020562515669127 28.733964263085195 22.75841329986405 40-41 22.745614712621826 25.77286749701772 28.84090175411495 22.640616036245504 42-43 22.70871767144987 25.926215663731917 28.43732371609201 22.927742948726202 44-45 22.5555539233656 26.128751508377345 28.61019006998739 22.70550449826967 46-47 22.836106584473118 26.548134949961995 27.908909996940185 22.706848468624706 48-49 22.827111988822278 26.323666150976514 27.87575377187131 22.9734680883299 50-51 22.952214120981246 26.652945279159436 27.629929854893938 22.764910744965388 52-53 23.26162522606142 26.411813188354756 27.51707570346815 22.809485882115673 54-55 22.68179939713779 26.563170479556685 27.64559267587112 23.109437447434402 56-57 22.679336558624794 26.82949250345838 27.553664728345055 22.93750620957177 58-59 22.79061160460555 26.276519651709634 27.590127734090437 23.342741009594377 60-61 22.464687547551705 26.581027113507176 27.911732154474674 23.04255318446645 62-63 22.533992187631526 26.656546613589484 27.819158821892458 22.990302376886536 64-65 22.22248815753377 26.587273421739035 27.644734926502267 23.54550349422493 66-67 22.182364585908797 26.756812338313317 27.690019089632806 23.37080398614508 68-69 22.532135000013206 26.345005242488188 27.813413638920654 23.309446118577952 70-71 22.403229211037978 26.570783647778605 27.419214023526912 23.606773117656505 72-73 22.491566943507806 26.708928634202156 27.222974156120845 23.576530266169197 74-75 22.40932869143775 27.025192022938754 26.996898249714835 23.56858103590866 76-77 22.434047036017578 26.796445301599515 27.273228636391405 23.496279025991505 78-79 22.47353408566758 26.871202793799466 27.282566454926144 23.37269666560681 80-81 22.46953741508502 27.151563214743867 27.05428169907375 23.324617671097354 82-83 22.52499762674228 27.051142671999273 26.985880148421476 23.43797955283697 84-85 22.448615778735878 26.936737819975303 26.876803317113108 23.737843084175704 86-87 22.606252690401178 26.933325586738533 26.770283974320037 23.690137748540252 88-89 22.418066580070906 27.011385844896687 26.61460239728426 23.95594517774815 90-91 22.26931367048015 27.054119563525337 26.849636587513086 23.826930178481433 92-93 22.38633894972191 26.60671919663875 26.90871261069295 24.09822924294639 94-95 22.145115711492043 26.578612597865213 27.022751382320887 24.253520308321857 96-97 22.298067806006937 26.394587855794803 26.947610302767245 24.359734035431014 98-99 22.111849200193724 26.69064167295104 26.579760433775995 24.617748693079243 100-101 22.118289406225582 26.413558008244564 26.931278013249276 24.536874572280578 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1258.0 1 910.0 2 709.0 3 914.0 4 813.5 5 789.0 6 1447.5 7 3015.0 8 4610.5 9 5027.0 10 4055.0 11 3152.5 12 3126.5 13 3324.0 14 3645.0 15 3851.5 16 4046.5 17 4360.5 18 4625.0 19 4995.0 20 5624.0 21 6557.5 22 7780.5 23 9327.0 24 10988.0 25 13166.5 26 15968.0 27 19335.0 28 22542.5 29 25848.5 30 30515.5 31 37201.0 32 46711.0 33 50325.0 34 49382.5 35 52175.5 36 56822.0 37 62022.5 38 66904.5 39 69995.5 40 73529.0 41 81870.5 42 89614.5 43 95171.0 44 100719.0 45 107171.0 46 110651.5 47 112816.0 48 115175.5 49 113028.5 50 111729.0 51 110902.0 52 109669.0 53 112410.0 54 116255.0 55 110249.0 56 98812.0 57 90731.5 58 84628.0 59 74331.0 60 60082.5 61 49883.5 62 42912.5 63 34146.0 64 25747.0 65 20123.0 66 15001.0 67 12092.5 68 9236.0 69 5899.0 70 4225.5 71 3202.0 72 2591.0 73 2167.0 74 1783.0 75 1475.0 76 1007.0 77 556.5 78 335.0 79 201.0 80 103.0 81 52.5 82 28.5 83 15.0 84 10.5 85 7.0 86 5.0 87 4.0 88 1.0 89 0.0 90 1.0 91 1.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.960922855416083E-4 2 0.051789066334565294 3 0.0 4 3.3007690461800694E-5 5 0.04423030521881293 6 3.3007690461800694E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.006419995794820236 26-27 0.0021950114157097463 28-29 1.3203076184720278E-4 30-31 0.0018814383563226396 32-33 4.125961307725087E-4 34-35 0.012344876232713459 36-37 0.023468467918340292 38-39 0.005033672795424606 40-41 0.0011552691661630243 42-43 0.0012542922375484264 44-45 0.008763541817608085 46-47 0.0 48-49 0.0 50-51 6.601538092360138E-4 52-53 0.0 54-55 0.001617376832628234 56-57 0.0 58-59 0.022692787192487976 60-61 0.0 62-63 0.009836291757616607 64-65 0.015051506850581117 66-67 0.006733568854207342 68-69 0.016536852921362147 70-71 8.251922615450173E-5 72-73 4.9511535692701035E-5 74-75 0.02185109108571206 76-77 0.049247474169006634 78-79 0.029376844511002618 80-81 0.02503633321527583 82-83 0.03497164804427784 84-85 0.015084514541042917 86-87 0.010199376352696415 88-89 0.08268426460681073 90-91 0.024376179406039814 92-93 0.023864560203881904 94-95 0.026868260035905766 96-97 0.037909332495378104 98-99 0.0522346701557996 100-101 0.014853460707810311 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3029597.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.12249474077076 #Duplication Level Percentage of deduplicated Percentage of total 1 69.4905674533344 29.271160620862545 2 15.007053676540053 12.642690791290459 3 5.7986147701104365 7.327563604731975 4 2.9671624256129623 4.999371346715785 5 1.713946497268452 3.609785115857642 6 1.0863720344638308 2.7456420184933887 7 0.7177189772356837 2.116247969077302 8 0.5334823434391378 1.7977285764647313 9 0.3700396804059419 1.4028295042598204 >10 2.0450684761957514 15.687030978050053 >50 0.1602659368591664 4.64733195551119 >100 0.09870990629319437 7.804844374744535 >500 0.006912916837195288 2.037332431008947 >1k 0.0038492377842585055 2.859975301957187 >5k 7.855587314813276E-5 0.2607136953653282 >10k+ 1.5711174629626552E-4 0.7897517156091238 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13282 0.43840814471363687 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10585 0.34938640353816036 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7879 0.2600675931485277 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3751 0.1238118469222144 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3449 0.1138435244027506 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.902307138540208E-5 2 0.0 0.0 0.0 0.0 9.902307138540208E-5 3 0.0 0.0 0.0 0.0 9.902307138540208E-5 4 0.0 0.0 0.0 0.0 9.902307138540208E-5 5 0.0 0.0 0.0 0.0 9.902307138540208E-5 6 0.0 0.0 0.0 0.0 1.3203076184720278E-4 7 0.0 0.0 0.0 0.0 1.3203076184720278E-4 8 0.0 0.0 0.0 0.0 1.3203076184720278E-4 9 0.0 0.0 0.0 0.0 1.3203076184720278E-4 10-11 0.0 0.0 0.0 1.6503845230900347E-5 1.3203076184720278E-4 12-13 3.3007690461800694E-5 0.0 0.0 3.3007690461800694E-5 3.7958844031070796E-4 14-15 6.601538092360139E-5 0.0 0.0 3.3007690461800694E-5 4.29099976003409E-4 16-17 8.251922615450173E-5 0.0 0.0 3.3007690461800694E-5 4.29099976003409E-4 18-19 1.4853460707810312E-4 0.0 0.0 3.3007690461800694E-5 5.281230473888111E-4 20-21 1.9804614277080417E-4 0.0 0.0 1.1552691661630244E-4 6.436499640051136E-4 22-23 2.3105383323260487E-4 0.0 0.0 3.465807498489073E-4 7.261691901596153E-4 24-25 2.3105383323260487E-4 0.0 0.0 6.931614996978147E-4 7.261691901596153E-4 26-27 2.3105383323260487E-4 0.0 0.0 0.0014028268446265296 8.416961067759177E-4 28-29 2.805653689253059E-4 0.0 0.0 0.0050831843311173065 8.912076424686188E-4 30-31 3.3007690461800697E-4 0.0 0.0 0.016503845230900347 9.077114876995191E-4 32-33 3.3007690461800697E-4 0.0 0.0 0.03977426700646984 9.572230233922201E-4 34-35 3.6308459507980765E-4 0.0 0.0 0.07675938416891752 9.902307138540207E-4 36-37 3.9609228554160833E-4 0.0 0.0 0.1380711692017123 9.902307138540207E-4 38-39 4.29099976003409E-4 0.0 0.0 0.22913938718582044 9.902307138540207E-4 40-41 4.29099976003409E-4 0.0 0.0 0.3280634354998371 9.902307138540207E-4 42-43 4.29099976003409E-4 0.0 0.0 0.42764763762308977 0.001039742249546722 44-45 4.951153569270104E-4 0.0 0.0 0.557466884209352 0.001089253785239423 46-47 5.281230473888111E-4 0.0 0.0 0.7103585064284128 0.001138765320932124 48-49 5.611307378506118E-4 0.0 0.0 0.8679042130025875 0.0012377883923175262 50-51 5.611307378506118E-4 0.0 0.0 1.0476145837218613 0.0013203076184720279 52-53 5.611307378506118E-4 0.0 0.0 1.2167789973385899 0.00141933068985743 54-55 5.611307378506118E-4 0.0 0.0 1.4050548637327012 0.0014523383803192306 56-57 5.941384283124125E-4 0.0 0.0 1.6246385245298303 0.0014853460707810312 58-59 5.941384283124125E-4 0.0 0.0 1.8602144113557018 0.0014853460707810312 60-61 6.106422735433128E-4 0.0 0.0 2.1187306430525252 0.0014853460707810312 62-63 6.271461187742132E-4 0.0 0.0 2.3747052825837893 0.0016338806778591344 64-65 6.271461187742132E-4 0.0 0.0 2.6340136988516956 0.0017329037492445365 66-67 6.931614996978146E-4 0.0 0.0 2.9460353967870976 0.0017494075944754369 68-69 6.931614996978146E-4 0.0 0.0 3.2858660739365666 0.0017494075944754369 70-71 6.931614996978146E-4 0.0 0.0 3.645814938422503 0.0017494075944754369 72-73 6.931614996978146E-4 0.0 0.0 4.016326263856216 0.0017824152849372375 74-75 6.931614996978146E-4 0.0 0.0 4.395683650333691 0.0018649345110917392 76-77 6.931614996978146E-4 0.0 0.0 4.806117777380952 0.0019309498920153407 78-79 6.931614996978146E-4 0.0 0.0 5.251820621686647 0.0019639575824771413 80-81 7.59176880621416E-4 0.0 0.0 5.734145498559709 0.0019804614277080415 82-83 7.59176880621416E-4 0.0 0.0 6.223434998120212 0.0020794844990934436 84-85 7.756807258523163E-4 0.0 0.0 6.72731389686483 0.0021124921895552444 86-87 7.921845710832167E-4 0.0 0.0 7.256278640360417 0.0021620037252479455 88-89 7.921845710832167E-4 0.0 0.0 7.816567682104253 0.0022115152609406465 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6575 0.0 51.684036 1 GTATCAA 10990 0.0 45.32207 1 TATCAAC 14845 0.0 33.520668 2 ATCAACG 15060 0.0 32.806572 3 TCAACGC 15305 0.0 32.28141 4 CAACGCA 15520 0.0 31.83421 5 AACGCAG 16010 0.0 30.875298 6 ACGCAGA 18375 0.0 26.746363 7 GTGGTAT 2535 0.0 26.435486 1 CGCAGAG 18520 0.0 26.38312 8 GTACATG 13370 0.0 26.269945 1 TACATGG 13595 0.0 25.275818 2 TGGTATC 2575 0.0 24.54825 2 ACATGGG 13990 0.0 23.702284 3 GCAGAGT 20230 0.0 23.660091 9 GTGTAAG 945 0.0 22.129265 1 GAGTACT 11845 0.0 21.767899 12-13 AGTACTT 12335 0.0 21.692345 12-13 AGAGTAC 18830 0.0 21.308758 10-11 GTATAGG 625 0.0 21.292377 1 >>END_MODULE