FastQCFastQC Report
Thu 26 May 2016
SRR938494_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938494_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3025496
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133080.4398617615095178No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101700.33614323072977126No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72960.24115054192767071No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA36770.12153379148410706No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA33250.10989933551391243No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67000.049.0351561
GTATCAA110700.044.6460461
TATCAAC145250.033.6055372
ATCAACG145550.033.601033
TCAACGC149000.032.8549164
CAACGCA150950.032.3973965
AACGCAG154550.031.6364696
GTACATG131750.028.4779591
GTGGTAT27250.027.9567551
TACATGG133350.027.6582852
ACGCAGA179800.026.9768687
CGCAGAG181700.026.6686298
ACATGGG139100.025.591993
TAAGGTG15950.025.3271375
TGGTATC28800.025.2496592
GCAGAGT195250.024.1102739
GTAAGGT14550.023.8456554
AGGTAAG14500.023.2727092
GGTAAGG15850.022.7894423
GAGTACT113600.022.72655312-13
Produced by FastQC (version 0.11.5)