##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938494_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3025496 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.985597072347808 31.0 31.0 34.0 28.0 34.0 2 31.072296244979334 31.0 31.0 34.0 27.0 34.0 3 31.13227814546772 31.0 31.0 34.0 27.0 34.0 4 34.77859696393583 37.0 35.0 37.0 32.0 37.0 5 34.7970891384421 37.0 35.0 37.0 32.0 37.0 6 34.831937639316 37.0 35.0 37.0 32.0 37.0 7 34.74348239098647 37.0 35.0 37.0 31.0 37.0 8 34.710746271024654 37.0 35.0 37.0 31.0 37.0 9 36.19612916361483 39.0 35.0 39.0 31.0 39.0 10-11 36.03327239566669 39.0 35.0 39.0 30.0 39.0 12-13 35.88076781459966 38.0 35.0 39.0 30.0 39.0 14-15 36.84037873459426 39.0 36.0 41.0 30.5 41.0 16-17 36.70017395494821 39.0 36.0 41.0 30.0 41.0 18-19 36.70359884792444 39.0 36.0 40.5 30.0 41.0 20-21 36.79594585482843 39.0 36.0 41.0 30.0 41.0 22-23 36.711966236279935 39.0 36.0 41.0 30.0 41.0 24-25 36.58879552311423 39.0 36.0 41.0 30.0 41.0 26-27 36.39437417864707 39.0 35.5 41.0 29.5 41.0 28-29 36.20069800125334 39.0 35.0 40.5 29.0 41.0 30-31 35.95887880863171 38.5 35.0 40.0 27.5 41.0 32-33 35.65916530710997 38.0 34.5 40.0 27.0 41.0 34-35 35.38939135930109 38.0 34.0 40.0 26.5 41.0 36-37 35.08672743246066 38.0 34.0 40.0 25.5 41.0 38-39 34.81703148839067 38.0 33.5 40.0 25.0 41.0 40-41 34.71707399381788 38.0 33.0 40.0 24.5 41.0 42-43 34.50436853990222 38.0 33.0 40.0 24.0 41.0 44-45 34.18170342978473 38.0 33.0 40.0 23.5 41.0 46-47 33.768744695084706 38.0 33.0 40.0 21.5 41.0 48-49 33.36473821151969 37.5 32.0 40.0 19.5 41.0 50-51 32.594364031550526 36.5 31.0 39.5 17.5 40.5 52-53 32.64000315981247 37.0 31.0 39.0 17.0 40.0 54-55 32.7540155399313 37.0 31.5 40.0 15.0 41.0 56-57 32.44233738864635 36.5 31.0 40.0 12.5 41.0 58-59 32.10202988204249 36.0 31.0 39.0 8.5 41.0 60-61 31.6485083768083 35.5 30.5 39.0 7.5 40.5 62-63 31.124603370819198 35.0 29.5 39.0 6.0 40.0 64-65 30.620897862697554 35.0 29.0 38.0 2.0 40.0 66-67 30.078769233210025 34.5 28.0 38.0 2.0 40.0 68-69 29.519855422053112 34.0 28.0 37.0 2.0 39.5 70-71 28.88587391951601 34.0 26.5 36.5 2.0 39.0 72-73 28.33297796460481 34.0 26.0 36.0 2.0 39.0 74-75 27.730609790923538 33.0 25.5 35.5 2.0 38.5 76-77 26.997042468408488 32.5 24.5 35.0 2.0 37.5 78-79 26.21152382948118 32.0 22.0 35.0 2.0 37.0 80-81 25.55713625137829 31.5 20.0 35.0 2.0 36.0 82-83 24.89170288111437 31.0 18.0 34.5 2.0 36.0 84-85 24.223189354737208 31.0 13.0 34.0 2.0 35.0 86-87 23.534986825300713 30.5 7.0 34.0 2.0 35.0 88-89 22.73094609941643 29.5 2.0 34.0 2.0 35.0 90-91 21.877155018549026 29.0 2.0 34.0 2.0 35.0 92-93 21.01858571288807 29.0 2.0 34.0 2.0 35.0 94-95 20.039865529486736 27.0 2.0 33.5 2.0 35.0 96-97 18.778722728438577 25.5 2.0 33.0 2.0 35.0 98-99 17.28377033055076 21.0 2.0 32.5 2.0 34.0 100-101 13.945851192663946 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6992759927529022 1101 2 0.7556415728261001 1101 3 0.6893038435819427 1101 4 0.6627712761015516 1101 5 0.596245989200419 1101 6 0.6677152672504221 1101 7 0.4795459111919058 1101 8 0.565547326124161 1101 9 0.689113009651237 1101 10-11 0.7847999707789484 1101 12-13 0.749875430449741 1101 14-15 0.9027545720748762 1101 16-17 1.0183050754744727 1101 18-19 0.9232342773472695 1101 20-21 0.9617763658462053 1101 22-23 1.0801349133457592 1101 24-25 1.028359538728587 1101 26-27 1.1188300440209886 1101 28-29 1.1219792856401725 1101 30-31 1.068928772256605 1101 32-33 1.2073232379770857 1101 34-35 1.1871550158182345 1101 36-37 1.198598910574134 1101 38-39 1.1894456687354307 1101 40-41 1.027077938043817 1101 42-43 1.1396897522665768 1101 44-45 1.2207821258194471 1101 46-47 1.1860176200759724 1101 48-49 1.2370362653241784 1101 50-51 1.0918145775371428 1101 52-53 1.2230549144262355 1101 54-55 1.3233180530399409 1101 56-57 1.2499138634442417 1101 58-59 1.254977832022405 1101 60-61 1.3056340320655373 1101 62-63 1.1525295502592456 1101 64-65 1.1510850356560205 1101 66-67 1.0451999997843764 1101 68-69 0.9320028429432767 1101 70-71 1.0344751253250628 1101 72-73 0.9502763464806883 1101 74-75 0.9321266918439086 1101 76-77 1.129175101999877 1101 78-79 1.1118735500027803 1101 80-81 1.2966600342241215 1101 82-83 1.4537576722682353 1101 84-85 1.3829780534811995 1101 86-87 1.2805011225859637 1101 88-89 1.592188125423739 1101 90-91 1.6598140635355634 1101 92-93 1.4403174945975188 1101 94-95 1.272838285602429 1101 96-97 1.3421355028572073 1101 98-99 1.3536732492171666 1101 100-101 1.0515995809787295 1102 1 0.5421908136225682 1102 2 0.6222887658604428 1102 3 0.6137353164385679 1102 4 0.6348507187710766 1102 5 0.5716322929804178 1102 6 0.6194255342693964 1102 7 0.4181454672255853 1102 8 0.45260066491769635 1102 9 0.6639633145666082 1102 10-11 0.6423605069619853 1102 12-13 0.6660140204179257 1102 14-15 0.7779993466953457 1102 16-17 0.7755409247252629 1102 18-19 0.7592769237895283 1102 20-21 0.752319845402404 1102 22-23 0.8750860288409257 1102 24-25 0.8057817444113127 1102 26-27 0.686891743205102 1102 28-29 0.638583130206456 1102 30-31 0.5971998685110549 1102 32-33 0.7271404350090762 1102 34-35 0.8880638575514936 1102 36-37 0.9598435113132098 1102 38-39 1.0734208735742072 1102 40-41 1.081593951923999 1102 42-43 1.2234351059499886 1102 44-45 1.2256326582431782 1102 46-47 1.1934523360101181 1102 48-49 1.1933043508846595 1102 50-51 1.2400280139642703 1102 52-53 1.3485725636581805 1102 54-55 1.274210441043536 1102 56-57 1.23722268871024 1102 58-59 1.302413392987603 1102 60-61 1.234333318014297 1102 62-63 1.1932752143290628 1102 64-65 1.142219183794296 1102 66-67 1.3162574559375564 1102 68-69 1.180726900219046 1102 70-71 1.1929422823264133 1102 72-73 1.2876913558572873 1102 74-75 1.136832011639413 1102 76-77 1.2218265954839254 1102 78-79 1.2996032251100083 1102 80-81 1.2624169789168427 1102 82-83 1.346539820553847 1102 84-85 1.3514142476780044 1102 86-87 1.4000083530183467 1102 88-89 1.52062834976865 1102 90-91 1.6763501972801436 1102 92-93 1.5784177781162718 1102 94-95 1.5360364112585465 1102 96-97 1.463053149705729 1102 98-99 1.5796010364383477 1102 100-101 1.5431137899592446 1103 1 0.4798664298834936 1103 2 0.5864151952025765 1103 3 0.4852629337881851 1103 4 0.44419217405112477 1103 5 0.4159444777512107 1103 6 0.5596948775702231 1103 7 0.4495633303573996 1103 8 0.44752787787833626 1103 9 0.414728807656239 1103 10-11 0.4910874375000347 1103 12-13 0.49747812568693206 1103 14-15 0.728677506467946 1103 16-17 0.7037631753338331 1103 18-19 0.6910026019201752 1103 20-21 0.6806812883382776 1103 22-23 0.7862253494244698 1103 24-25 0.7972888122883788 1103 26-27 0.8243989438681467 1103 28-29 0.7209812994682068 1103 30-31 0.8518056720869325 1103 32-33 0.9559396187246989 1103 34-35 0.9148259282311741 1103 36-37 0.8458345121506881 1103 38-39 0.7731905947197575 1103 40-41 0.9274672132655795 1103 42-43 0.8138471553593121 1103 44-45 0.9014099836512841 1103 46-47 0.8455228332030416 1103 48-49 0.8168635226366021 1103 50-51 0.9446011969673052 1103 52-53 0.9796776290811664 1103 54-55 0.9909543138309331 1103 56-57 1.063676582012171 1103 58-59 1.0037783578582093 1103 60-61 1.1745112900616839 1103 62-63 1.1502443366693527 1103 64-65 1.0207548209774302 1103 66-67 1.1345077378489385 1103 68-69 1.085580240944907 1103 70-71 1.2427114663746508 1103 72-73 1.3685056057348852 1103 74-75 1.1672856233211633 1103 76-77 1.2376600947997431 1103 78-79 1.2253410447459991 1103 80-81 1.237737616305104 1103 82-83 1.346025695476957 1103 84-85 1.2712894883849586 1103 86-87 1.2406528749687347 1103 88-89 1.185266794624738 1103 90-91 1.4064861061956861 1103 92-93 1.402573865583669 1103 94-95 1.2527518761279204 1103 96-97 1.2217689772128502 1103 98-99 1.208297835170125 1103 100-101 1.0364159276453702 1104 1 0.37871524389192857 1104 2 0.5453558812366914 1104 3 0.4780699039643679 1104 4 0.5138504671759065 1104 5 0.4005279036851306 1104 6 0.43246724420854576 1104 7 0.45498883693617387 1104 8 0.39414659427809795 1104 9 0.47323866372584433 1104 10-11 0.6126893021237905 1104 12-13 0.57796649352278 1104 14-15 0.7463284331778723 1104 16-17 0.7520619877810617 1104 18-19 0.8638394981567075 1104 20-21 0.7619604804815836 1104 22-23 0.828641560435031 1104 24-25 0.7380597022152102 1104 26-27 0.7836633799168098 1104 28-29 0.7780391171310796 1104 30-31 0.7885975837242398 1104 32-33 0.839596855131397 1104 34-35 0.8469178320930908 1104 36-37 0.8702962502009299 1104 38-39 0.7949797421147835 1104 40-41 0.7772530839774134 1104 42-43 0.7692381012566898 1104 44-45 0.8274161464115863 1104 46-47 0.868853895143495 1104 48-49 0.8830614007515791 1104 50-51 0.9720038683947152 1104 52-53 1.008091468292264 1104 54-55 1.1195694560011518 1104 56-57 1.1037399116292335 1104 58-59 0.9585989453714276 1104 60-61 1.06036963473667 1104 62-63 1.0150501142112205 1104 64-65 1.1437754796323851 1104 66-67 1.0851875332888845 1104 68-69 1.2125489600078971 1104 70-71 1.3778077924119785 1104 72-73 1.1905323004174377 1104 74-75 1.072789312344824 1104 76-77 1.2985145058630785 1104 78-79 1.2583419955473865 1104 80-81 1.2193410482511737 1104 82-83 1.1382275323052369 1104 84-85 1.1772368030904197 1104 86-87 1.2685979256281463 1104 88-89 1.2007598981004577 1104 90-91 1.3891405012184599 1104 92-93 1.4359320556692516 1104 94-95 1.243839077410673 1104 96-97 1.178380781476335 1104 98-99 1.016668193431844 1104 100-101 0.672624011722414 1105 1 0.45151357247483404 1105 2 0.591372799406706 1105 3 0.5671734885030695 1105 4 0.5645562695741546 1105 5 0.43439988624664494 1105 6 0.5152064016007003 1105 7 0.4041465385313785 1105 8 0.4399954997314026 1105 9 0.4928395361083844 1105 10-11 0.5894514586919328 1105 12-13 0.5802256113070356 1105 14-15 0.7641143441769813 1105 16-17 0.6562808719061621 1105 18-19 0.6360227924666191 1105 20-21 0.569528960562451 1105 22-23 0.7850816849890663 1105 24-25 0.6630935397013005 1105 26-27 0.7015515769661533 1105 28-29 0.743944855808266 1105 30-31 0.7017174744357462 1105 32-33 0.6983790723720702 1105 34-35 0.6695241379564578 1105 36-37 0.905043022030263 1105 38-39 0.7823275020789353 1105 40-41 0.7501420660793556 1105 42-43 0.7992233285150974 1105 44-45 0.7616906260133973 1105 46-47 0.7348181975984716 1105 48-49 0.8038054128631629 1105 50-51 0.6389681674115053 1105 52-53 0.6389290793027058 1105 54-55 0.742421706809985 1105 56-57 0.7344238357003263 1105 58-59 0.8207481787131243 1105 60-61 0.9154659440726114 1105 62-63 1.000711329060053 1105 64-65 0.9015697912673204 1105 66-67 0.8491774571101338 1105 68-69 0.7664047143288357 1105 70-71 0.9460869112735253 1105 72-73 0.817494043715552 1105 74-75 0.9383746120343552 1105 76-77 1.0319129928317778 1105 78-79 1.0491308645329944 1105 80-81 1.189634142195036 1105 82-83 1.1725778812238694 1105 84-85 1.2996950950222619 1105 86-87 1.325797774806361 1105 88-89 1.323861188222665 1105 90-91 1.1218208656203927 1105 92-93 1.1266851675146086 1105 94-95 1.130781016754188 1105 96-97 0.9710919405692486 1105 98-99 1.0947772022828808 1105 100-101 1.076193021284011 1106 1 0.3175107608877248 1106 2 0.34860078278532924 1106 3 0.19107286514607225 1106 4 0.3644690860023303 1106 5 0.4489644170450475 1106 6 -0.027050489723372095 1106 7 0.21819118050822084 1106 8 0.41965370024918514 1106 9 0.3761292038027122 1106 10-11 0.14930386698578246 1106 12-13 0.22814367425866777 1106 14-15 0.5074588145561663 1106 16-17 0.26186704572161545 1106 18-19 -0.017082360669604668 1106 20-21 0.3992246599862881 1106 22-23 0.4079251101624095 1106 24-25 0.3965737483658174 1106 26-27 0.11149127961146377 1106 28-29 0.18525840316046782 1106 30-31 0.35712169270264127 1106 32-33 0.5244215918111195 1106 34-35 0.3002505310934396 1106 36-37 0.14916488848984955 1106 38-39 0.18210566586219556 1106 40-41 0.38108647353571 1106 42-43 -0.007395089928380116 1106 44-45 0.19936190517474728 1106 46-47 -0.09211847965503495 1106 48-49 0.3541637849785957 1106 50-51 0.01856477445287652 1106 52-53 0.12666992807410793 1106 54-55 -0.07729319072439722 1106 56-57 -0.3628987867194837 1106 58-59 -0.6983523349720357 1106 60-61 0.08407869503002274 1106 62-63 0.20489674979556582 1106 64-65 0.3277493451134248 1106 66-67 -0.28775037992859254 1106 68-69 -0.3684063454666848 1106 70-71 -0.4030220881315856 1106 72-73 0.06576909403720066 1106 74-75 0.024021504167116348 1106 76-77 0.11473987860578916 1106 78-79 0.4012223286298706 1106 80-81 0.43481877307175765 1106 82-83 0.33943737830648857 1106 84-85 0.16752574176483748 1106 86-87 0.3236098770382405 1106 88-89 0.045535445140572506 1106 90-91 0.1124983717049659 1106 92-93 0.20139406633181167 1106 94-95 -0.11176395700765696 1106 96-97 0.12536986229900648 1106 98-99 -0.0829921500883053 1106 100-101 -0.6255292142933104 1107 1 0.4131043303699009 1107 2 0.4619352014201681 1107 3 0.3298827150020571 1107 4 0.37431404749008834 1107 5 0.33046215440420923 1107 6 0.24615075603251313 1107 7 0.3518856425976793 1107 8 0.31154522117156347 1107 9 0.40941199613452994 1107 10-11 0.512810497433172 1107 12-13 0.47065514706601164 1107 14-15 0.5427569692243992 1107 16-17 0.5894583360706278 1107 18-19 0.47195066760888693 1107 20-21 0.5279270543529293 1107 22-23 0.5761220519530141 1107 24-25 0.47303148385231 1107 26-27 0.5481701612401793 1107 28-29 0.5415862429529454 1107 30-31 0.6601553232170758 1107 32-33 0.5141321355152613 1107 34-35 0.5991110709138994 1107 36-37 0.6298848796371885 1107 38-39 0.656851763830872 1107 40-41 0.46639388518738656 1107 42-43 0.6781849239251727 1107 44-45 0.7857193460337939 1107 46-47 0.6694521112277982 1107 48-49 0.5482438376689203 1107 50-51 0.5942181099993746 1107 52-53 0.6778879130096982 1107 54-55 0.6203814975972293 1107 56-57 0.6698197552311456 1107 58-59 0.677697774036929 1107 60-61 0.6681679089418857 1107 62-63 0.781524090242943 1107 64-65 0.7556410618156839 1107 66-67 0.7102062739804573 1107 68-69 0.7209203526311647 1107 70-71 0.8094863309352434 1107 72-73 0.6565989347213232 1107 74-75 0.8495643943250286 1107 76-77 0.8814274532867685 1107 78-79 0.8796761676711462 1107 80-81 0.783313183236988 1107 82-83 0.9532833250777664 1107 84-85 0.9097812110992187 1107 86-87 0.696056093432464 1107 88-89 0.8863987492878067 1107 90-91 1.2324627057182056 1107 92-93 1.2182974776898128 1107 94-95 1.0047009180603048 1107 96-97 0.9185654474627221 1107 98-99 0.8451710515937023 1107 100-101 0.3740629996260001 1108 1 0.3692258835764832 1108 2 0.27417590274417236 1108 3 0.3330117360105085 1108 4 0.343211434360704 1108 5 0.34623292535554384 1108 6 0.2816721192370579 1108 7 0.4388109766044437 1108 8 0.2934932231437415 1108 9 0.21279776041173903 1108 10-11 0.32668545684729366 1108 12-13 0.38140198738011577 1108 14-15 0.5517745504262237 1108 16-17 0.47839642907543123 1108 18-19 0.5633115873149848 1108 20-21 0.5464188830624792 1108 22-23 0.5650706672229262 1108 24-25 0.45127865486617225 1108 26-27 0.4951592902371402 1108 28-29 0.4133447840258526 1108 30-31 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-1.8707073129341794 2316 72-73 -1.6485856849040523 2316 74-75 -1.360907229076311 2316 76-77 -1.42939713655678 2316 78-79 -1.3948233787466506 2316 80-81 -1.4759397661411953 2316 82-83 -1.2351542847627748 2316 84-85 -1.1818875156474 2316 86-87 -1.3065801708294877 2316 88-89 -1.6059870675904087 2316 90-91 -1.6845464264521475 2316 92-93 -1.682119168922977 2316 94-95 -1.8425644807638797 2316 96-97 -1.7312756529189564 2316 98-99 -1.5815286602138734 2316 100-101 -1.3105090433753421 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8893.0 3 3241.0 4 3501.0 5 4031.0 6 4831.0 7 6350.0 8 8057.0 9 10244.0 10 12983.0 11 16468.0 12 20553.0 13 24202.0 14 27342.0 15 32440.0 16 37699.0 17 43288.0 18 40731.0 19 37718.0 20 39599.0 21 42543.0 22 46693.0 23 52751.0 24 58554.0 25 65234.0 26 73647.0 27 83445.0 28 95054.0 29 108187.0 30 124635.0 31 145210.0 32 170183.0 33 202939.0 34 246328.0 35 300552.0 36 345874.0 37 327382.0 38 146681.0 39 7433.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.56901170410528 18.04464023767155 13.862805077937965 23.52354298028521 2 15.847646673293427 19.83497911115432 39.30280790793432 25.01456630761793 3 19.178109370479064 23.96484933658496 29.473880874633156 27.383160418302815 4 13.077898869433977 15.961424245991696 35.65548887357423 35.3051880110001 5 14.070480554647927 37.17128630749306 33.345547722765986 15.412685415093016 6 33.31331288292832 34.895512762210295 16.947289218888237 14.843885135973146 7 29.405560184634105 32.707250459601376 19.76841381317039 18.118775542594136 8 27.405903117143165 33.98967609167462 19.35908050294336 19.245340288238864 9 27.48935835325869 16.026437378707712 19.348205130409564 37.135999137624026 10-11 25.590574028316603 26.06808913769686 26.74063119224191 21.60070564174463 12-13 26.46716595575836 23.437175544754158 27.27173230168965 22.823926197797835 14-15 23.039662455907045 24.848661791154676 25.642264694441224 26.46941105849705 16-17 22.579955635950288 28.236586884336397 26.313538573546026 22.869918906167293 18-19 22.638996123037753 26.131271131097535 29.036314488596442 22.19341825726827 20-21 23.238553541146935 25.45725980756563 29.07491157384014 22.229275077447294 22-23 22.867358954577576 25.448359649334556 29.049604854899624 22.63467654118824 24-25 22.64244644041623 25.735638603019666 29.219671977161404 22.402242979402697 26-27 22.194847876165955 26.162101617834878 28.933307715354715 22.709742790644455 28-29 22.288960813503543 26.385263448885212 29.010807650668585 22.314968086942663 30-31 22.353274598969474 25.90911661342962 28.860887126777136 22.876721660823765 32-33 22.152107193157143 26.03699604398017 28.84210609858006 22.968790664282626 34-35 22.87158152044784 26.17105984791454 28.59347819221643 22.363880439421187 36-37 23.14358647666133 25.708175730851966 28.483720973297594 22.66451681918911 38-39 22.463872690235068 25.991198093157124 28.594559665790047 22.95036955081776 40-41 22.76621289295248 25.813775076283278 28.72692925735405 22.693082773410193 42-43 22.653753351627433 25.897217915745195 28.36062378908744 23.088404943539924 44-45 22.42540812457877 26.166496384070275 28.56126686285249 22.846828628498464 46-47 22.793836790275638 26.558648686037973 27.818786838116328 22.828727685570062 48-49 22.808129564227432 26.332774243708425 27.809061747559465 23.05003444450468 50-51 22.9111477952391 26.681758652080866 27.547214654376234 22.859878898303794 52-53 23.135438068527495 26.454844473636324 27.506115722476686 22.903601735359494 54-55 22.571973242997377 26.606580429677585 27.653370805830583 23.16807552149446 56-57 22.62771128862609 26.830328484983912 27.576934849739953 22.965025376650043 58-59 22.72363656197265 26.194356340893805 27.67292032004866 23.409086777084887 60-61 22.439653345771298 26.496960313002972 27.9778635550603 23.085522786165434 62-63 22.47646409803998 26.59884153319023 27.909338932192934 23.01535543657685 64-65 22.212193802183 26.52381208948164 27.794070058053922 23.469924050281442 66-67 22.152704680790862 26.65441033683257 27.799491335868886 23.393393646507683 68-69 22.505990475400292 26.201111390613374 27.97856948538271 23.31432864860362 70-71 22.368210728609654 26.396319866410185 27.607580911216235 23.62788849376393 72-73 22.412708708244093 26.51859830112634 27.465129683378205 23.603563307251363 74-75 22.279734164573195 26.829155810860534 27.39626940449519 23.494840620071084 76-77 22.44079267822029 26.484015547856664 27.446310656648524 23.62888111727452 78-79 22.481184380428616 26.60378789794422 27.40088694002057 23.514140781606596 80-81 22.514896884504232 26.804905435730774 27.24815117325671 23.432046506508286 82-83 22.503207505041193 26.682607645819385 27.20737745165687 23.60680739748255 84-85 22.478882122155216 26.550838343928014 27.155167213464804 23.81511232045197 86-87 22.624933806948334 26.488178466383843 27.04380343787373 23.843084288794085 88-89 22.572514440069167 26.44954489398493 26.934837018154646 24.043103647791256 90-91 22.432151695449193 26.50428162772777 27.061305714187938 24.0022609626351 92-93 22.573728136948123 25.987777684107517 27.23949887513038 24.198995303813977 94-95 22.367272539713923 25.882193654104174 27.390043200443316 24.36049060573859 96-97 22.588213705021072 25.699026501928895 27.215838500331884 24.49692129271815 98-99 22.508147969136154 25.819057478700895 26.865577890485543 24.807216661677412 100-101 22.812336810085164 25.131213792108376 27.18112732006833 24.875322077738133 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1362.0 1 940.0 2 716.0 3 949.0 4 801.5 5 814.5 6 1454.5 7 2884.5 8 4570.0 9 5100.5 10 4192.0 11 3344.5 12 3275.5 13 3503.0 14 3792.5 15 3949.0 16 4142.5 17 4434.5 18 4762.0 19 5129.0 20 5672.0 21 6667.0 22 7960.5 23 9428.5 24 11074.5 25 13177.5 26 16030.0 27 19355.5 28 22268.5 29 25484.5 30 30070.0 31 36756.0 32 45623.0 33 49228.5 34 49475.5 35 52600.5 36 56687.5 37 61636.5 38 66566.5 39 69694.5 40 73229.5 41 80777.0 42 88305.0 43 93749.0 44 98655.5 45 104986.0 46 108818.5 47 110618.5 48 112615.0 49 111344.0 50 109931.5 51 108839.0 52 108991.0 53 111851.5 54 114347.0 55 108819.5 56 98772.0 57 91480.0 58 85313.5 59 75873.0 60 62616.5 61 52260.0 62 44693.5 63 35787.5 64 27626.5 65 21793.5 66 16712.5 67 13299.5 68 10143.0 69 6981.0 70 5248.0 71 3950.5 72 3141.0 73 2605.0 74 1978.5 75 1560.0 76 1129.5 77 698.0 78 431.5 79 245.5 80 135.5 81 85.0 82 55.5 83 35.5 84 19.0 85 12.0 86 7.0 87 8.5 88 7.0 89 4.5 90 6.0 91 5.5 92 2.0 93 0.5 94 0.5 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18277994748629647 2 0.04607508983650945 3 0.04531488390663878 4 0.05037190596186542 5 0.05751123121630305 6 0.06349372136006791 7 0.037547562449264514 8 0.035035577637517946 9 0.0427367942314252 10-11 0.04291858260595949 12-13 0.03953070835327497 14-15 0.035085156285118206 16-17 0.03222612094016981 18-19 0.040357019146612653 20-21 0.04971085732719528 22-23 0.046835295766380125 24-25 0.06668328102235137 26-27 0.05215673727547483 28-29 0.04435636338636706 30-31 0.05285083834187849 32-33 0.04947949030506073 34-35 0.04866970572758979 36-37 0.04645519280144479 38-39 0.026788995920007826 40-41 0.024805850015997378 42-43 0.021798078728248193 44-45 0.004395973420556498 46-47 0.011617929754327884 48-49 0.011237826789392549 50-51 0.008924156568047025 52-53 0.00996530816765251 54-55 0.011370036516326579 56-57 0.009056366294981055 58-59 0.010940354903790982 60-61 0.011535298674994117 62-63 0.0039001869445538847 64-65 0.006775748505369036 66-67 0.003982818023887654 68-69 0.009155523590181576 70-71 0.023615962473591105 72-73 0.02014545714157282 74-75 0.020393350379574127 76-77 0.012708660001533633 78-79 0.004974390975892878 80-81 0.006577433914967993 82-83 0.005602387178829521 84-85 0.005172705566293923 86-87 0.008395317660310904 88-89 0.030606551785227944 90-91 0.03703524975739515 92-93 0.023169754645188757 94-95 0.032721907416172424 96-97 0.03614283410059045 98-99 0.02189723602344872 100-101 0.12262452173131282 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3025496.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.28386536488448 #Duplication Level Percentage of deduplicated Percentage of total 1 72.43704686507256 32.80229477667773 2 13.637876579458435 12.351515337742141 3 5.342048970873653 7.257258791089866 4 2.6109896867932982 4.729428217833984 5 1.5125942191062391 3.424805648485475 6 0.9697379526465537 2.6348089732119155 7 0.6465223767089001 2.0493922583589668 8 0.4895881952573266 1.7736356734615644 9 0.33326782101467867 1.3582489623549414 >10 1.7916696844023978 14.810217958368508 >50 0.13364913606011905 4.138235055293812 >100 0.08589454056265317 7.223307198491474 >500 0.00521848399261167 1.6508302482576713 >1k 0.0036749887270196495 2.7717532728364547 >5k 7.349977454039299E-5 0.2428367001526805 >10k+ 1.4699954908078597E-4 0.7814309273827621 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13308 0.4398617615095178 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10170 0.33614323072977126 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7296 0.24115054192767071 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3677 0.12153379148410706 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3325 0.10989933551391243 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.30524317335075E-5 2 0.0 0.0 0.0 0.0 3.30524317335075E-5 3 0.0 0.0 0.0 0.0 3.30524317335075E-5 4 0.0 0.0 0.0 0.0 3.30524317335075E-5 5 0.0 0.0 0.0 0.0 3.30524317335075E-5 6 3.30524317335075E-5 0.0 0.0 0.0 3.30524317335075E-5 7 9.915729520052249E-5 0.0 0.0 0.0 3.30524317335075E-5 8 9.915729520052249E-5 0.0 0.0 0.0 3.30524317335075E-5 9 9.915729520052249E-5 0.0 0.0 0.0 3.30524317335075E-5 10-11 9.915729520052249E-5 0.0 0.0 0.0 3.30524317335075E-5 12-13 9.915729520052249E-5 0.0 0.0 3.30524317335075E-5 2.809456697348137E-4 14-15 9.915729520052249E-5 0.0 0.0 3.30524317335075E-5 3.6357674906858245E-4 16-17 9.915729520052249E-5 0.0 0.0 6.6104863467015E-5 3.9662918080208995E-4 18-19 1.8178837453429122E-4 0.0 0.0 8.263107933376875E-5 4.2968161253559745E-4 20-21 2.3136702213455247E-4 0.0 0.0 1.4873594280078375E-4 5.2883890773612E-4 22-23 2.3136702213455247E-4 0.0 0.0 4.6273404426910495E-4 5.2883890773612E-4 24-25 2.974718856015675E-4 0.0 0.0 9.419943044049637E-4 5.618913394696274E-4 26-27 2.974718856015675E-4 0.0 0.0 0.0015534642914748523 5.94943771203135E-4 28-29 3.470505332018287E-4 0.0 0.0 0.0050570220552266475 6.279962029366424E-4 30-31 3.6357674906858245E-4 0.0 0.0 0.016162639117685167 6.6104863467015E-4 32-33 3.6357674906858245E-4 0.0 0.0 0.039695970511942506 6.941010664036574E-4 34-35 4.131553966688437E-4 0.0 0.0 0.07572312110146567 7.602059298706724E-4 36-37 4.6273404426910495E-4 0.0 0.0 0.13844011031579617 7.602059298706724E-4 38-39 4.957864760026124E-4 0.0 0.0 0.23035892296668048 7.767321457374262E-4 40-41 4.957864760026124E-4 0.0 0.0 0.32900390547533365 7.932583616041799E-4 42-43 5.618913394696274E-4 0.0 0.0 0.43212749248387705 8.097845774709337E-4 44-45 6.279962029366424E-4 0.0 0.0 0.558437360353476 8.593632250711949E-4 46-47 6.6104863467015E-4 0.0 0.0 0.7124286397998874 8.924156568047024E-4 48-49 6.6104863467015E-4 0.0 0.0 0.8738732426022048 9.585205202717174E-4 50-51 6.6104863467015E-4 0.0 0.0 1.04948081240233 0.00105767781547224 52-53 6.6104863467015E-4 0.0 0.0 1.2231217625143116 0.001123782678939255 54-55 6.6104863467015E-4 0.0 0.0 1.407851803472885 0.0011568351106727623 56-57 7.932583616041799E-4 0.0 0.0 1.629732777699921 0.00118988754240627 58-59 7.932583616041799E-4 0.0 0.0 1.8618269533326106 0.00118988754240627 60-61 7.932583616041799E-4 0.0 0.0 2.1221479056657158 0.00118988754240627 62-63 7.932583616041799E-4 0.0 0.0 2.3757592143569184 0.001255992405873285 64-65 7.932583616041799E-4 0.0 0.0 2.644310222191667 0.001255992405873285 66-67 8.263107933376874E-4 0.0 0.0 2.9563582301877114 0.001255992405873285 68-69 8.263107933376874E-4 0.0 0.0 3.2999713104892554 0.001255992405873285 70-71 8.428370092044412E-4 0.0 0.0 3.658821561819946 0.001255992405873285 72-73 9.089418726714562E-4 0.0 0.0 4.031239836377242 0.0012890448376067926 74-75 9.254680885382099E-4 0.0 0.0 4.4062857792573515 0.0013551497010738075 76-77 9.254680885382099E-4 0.0 0.0 4.813392580918963 0.0014047283486740686 78-79 9.254680885382099E-4 0.0 0.0 5.254675596993022 0.0014873594280078372 80-81 9.91572952005225E-4 0.0 0.0 5.7211610922638805 0.001520411859741345 82-83 0.0010246253837387323 0.0 0.0 6.205775846340567 0.0016195691549418675 84-85 0.001041151599605486 0.0 0.0 6.693183530898735 0.0016195691549418675 86-87 0.0010742040313389937 0.0 0.0 7.1976297440155275 0.001652621586675375 88-89 0.0011072564630725012 0.0 0.0 7.739061628242113 0.0016856740184088823 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6700 0.0 49.035156 1 GTATCAA 11070 0.0 44.646046 1 TATCAAC 14525 0.0 33.605537 2 ATCAACG 14555 0.0 33.60103 3 TCAACGC 14900 0.0 32.854916 4 CAACGCA 15095 0.0 32.397396 5 AACGCAG 15455 0.0 31.636469 6 GTACATG 13175 0.0 28.477959 1 GTGGTAT 2725 0.0 27.956755 1 TACATGG 13335 0.0 27.658285 2 ACGCAGA 17980 0.0 26.976868 7 CGCAGAG 18170 0.0 26.668629 8 ACATGGG 13910 0.0 25.59199 3 TAAGGTG 1595 0.0 25.327137 5 TGGTATC 2880 0.0 25.249659 2 GCAGAGT 19525 0.0 24.110273 9 GTAAGGT 1455 0.0 23.845655 4 AGGTAAG 1450 0.0 23.272709 2 GGTAAGG 1585 0.0 22.789442 3 GAGTACT 11360 0.0 22.726553 12-13 >>END_MODULE