FastQCFastQC Report
Thu 26 May 2016
SRR938494_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938494_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3025496
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129810.42905361633266087No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105860.3498930423309104No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79000.2611142106947092No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA37130.12272367902651334No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA34630.11446057109313647No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66500.050.6358531
GTATCAA110850.044.8789331
TATCAAC147950.033.528082
ATCAACG151500.032.63263
TCAACGC154800.031.9983624
CAACGCA157300.031.4898075
AACGCAG162400.030.532176
ACGCAGA188000.026.121977
CGCAGAG189850.025.8424058
GTACATG135450.025.4920141
TACATGG137700.024.5569822
GTGGTAT26850.024.090241
GCAGAGT207100.023.2083179
ACATGGG143000.022.7711773
TGGTATC28800.021.633072
AGTACTT117800.021.63055412-13
GAGTACT114450.021.26773612-13
AGAGTAC190250.020.94500510-11
GTGTAAG11150.020.4744831
CAGAGTA196550.019.9836910-11
Produced by FastQC (version 0.11.5)