##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938494_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3025496 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.79503426876122 31.0 31.0 34.0 30.0 34.0 2 32.02365463381872 31.0 31.0 34.0 30.0 34.0 3 32.0849692083546 33.0 31.0 34.0 30.0 34.0 4 35.76348506162295 37.0 35.0 37.0 35.0 37.0 5 35.63290581114634 37.0 35.0 37.0 33.0 37.0 6 35.660341973679685 37.0 35.0 37.0 33.0 37.0 7 35.59496889105125 37.0 35.0 37.0 33.0 37.0 8 35.58431708387649 37.0 35.0 37.0 33.0 37.0 9 37.15829007871767 39.0 37.0 39.0 33.0 39.0 10-11 37.072921431725575 39.0 37.0 39.0 33.0 39.0 12-13 36.96401664388252 39.0 37.0 39.0 33.0 39.0 14-15 38.01883740715572 40.0 37.0 41.0 33.0 41.0 16-17 37.95415578139915 40.0 37.0 41.0 33.0 41.0 18-19 38.06804702435568 40.0 37.0 41.0 33.0 41.0 20-21 38.11713005074209 40.0 37.0 41.0 33.0 41.0 22-23 38.02193078424166 40.0 37.0 41.0 33.0 41.0 24-25 37.92504237321748 40.0 37.0 41.0 33.0 41.0 26-27 37.736589636872765 40.0 37.0 41.0 32.0 41.0 28-29 37.61439198729729 40.0 37.0 41.0 32.0 41.0 30-31 37.36603634577603 39.0 36.0 41.0 31.5 41.0 32-33 37.13898382281781 39.0 36.0 41.0 31.0 41.0 34-35 36.97330950032656 39.0 35.5 41.0 31.0 41.0 36-37 36.947102392467215 39.0 35.5 41.0 31.0 41.0 38-39 36.8152704548279 39.0 35.0 41.0 30.0 41.0 40-41 36.630032728517904 39.0 35.0 41.0 30.0 41.0 42-43 36.401730327853684 39.0 35.0 40.5 30.0 41.0 44-45 36.11186876465875 39.0 35.0 40.0 29.5 41.0 46-47 35.7111123267061 38.0 35.0 40.0 28.5 41.0 48-49 35.3245798374878 38.0 34.0 40.0 27.0 41.0 50-51 34.95217627126263 38.0 34.0 40.0 26.0 41.0 52-53 34.47041989148225 38.0 33.0 40.0 24.0 41.0 54-55 34.16093543009146 37.5 33.0 40.0 24.0 41.0 56-57 33.83133426717471 37.0 33.0 40.0 23.0 41.0 58-59 33.3955711724623 36.5 32.0 39.5 22.5 41.0 60-61 33.03925769526715 36.0 32.0 39.0 22.0 40.5 62-63 32.59299615666324 35.5 31.0 39.0 20.5 40.0 64-65 32.10922242171201 35.0 31.0 38.5 19.0 40.0 66-67 31.637518773781224 35.0 30.5 38.0 18.0 40.0 68-69 31.122510325579675 34.5 30.0 37.5 14.5 40.0 70-71 30.570206339720826 34.0 29.0 37.0 12.0 39.0 72-73 30.158029460293452 34.0 29.0 36.0 8.0 39.0 74-75 29.675514692466955 34.0 29.0 36.0 7.0 38.5 76-77 28.40123057508587 32.5 27.0 34.5 7.0 36.5 78-79 29.108878345897665 33.5 28.5 35.0 3.5 37.0 80-81 28.86744057833823 34.0 28.0 35.0 2.0 37.0 82-83 28.428242013871444 33.0 27.5 35.0 2.0 36.0 84-85 27.944470427328277 33.0 26.5 35.0 2.0 36.0 86-87 27.401679096584495 33.0 26.0 35.0 2.0 35.0 88-89 26.800590547797782 32.0 25.0 34.5 2.0 35.0 90-91 26.300225153164966 32.0 24.5 34.0 2.0 35.0 92-93 25.6069046199367 32.0 22.5 34.0 2.0 35.0 94-95 24.88699208328155 31.0 19.0 34.0 2.0 35.0 96-97 23.93979202087856 31.0 8.5 34.0 2.0 35.0 98-99 22.75747629479596 30.5 2.0 34.0 2.0 35.0 100-101 19.14347019463916 25.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.33327079089984935 1101 2 0.3418292321253915 1101 3 0.3710885491730309 1101 4 0.2204089256179671 1101 5 0.18627208554689645 1101 6 0.20536943534893481 1101 7 0.280811705564183 1101 8 0.30556055854578545 1101 9 0.36349814384453083 1101 10-11 0.36848291954204626 1101 12-13 0.43708409653586244 1101 14-15 0.3593558406146471 1101 16-17 0.48811587846822846 1101 18-19 0.49818640606479647 1101 20-21 0.49454696602708736 1101 22-23 0.4268751456632529 1101 24-25 0.49751585190281133 1101 26-27 0.505355487429803 1101 28-29 0.5510225677865535 1101 30-31 0.47089885886308025 1101 32-33 0.4114905358676566 1101 34-35 0.37439486034720204 1101 36-37 0.4422315197654143 1101 38-39 0.49547910201495426 1101 40-41 0.45697444103796414 1101 42-43 0.6193751480129137 1101 44-45 0.6310048394210597 1101 46-47 0.5218515134948021 1101 48-49 0.4435666949337147 1101 50-51 0.45518829267765426 1101 52-53 0.4134251723309319 1101 54-55 0.3651669932986792 1101 56-57 0.3245976700664457 1101 58-59 0.47146025633313826 1101 60-61 0.5252945397246265 1101 62-63 0.44434205909202973 1101 64-65 0.5336248902663954 1101 66-67 0.4840289041347532 1101 68-69 0.5641787696220248 1101 70-71 0.4408586073832659 1101 72-73 0.3898386595322947 1101 74-75 0.4866579740041921 1101 76-77 0.3738565170056809 1101 78-79 0.43358278281925067 1101 80-81 0.5108880868078245 1101 82-83 0.5557564260981316 1101 84-85 0.48384355475167595 1101 86-87 0.5198220169801928 1101 88-89 0.5752387625444229 1101 90-91 0.6053441918323763 1101 92-93 0.6035177071438369 1101 94-95 0.6533788130869773 1101 96-97 0.7495093541165616 1101 98-99 0.6137230372382625 1101 100-101 0.3732572173092059 1102 1 0.3084045333148815 1102 2 0.33963959957306145 1102 3 0.3517323274152133 1102 4 0.2764783611697226 1102 5 0.1911937143334228 1102 6 0.23620941246809224 1102 7 0.29398146038600714 1102 8 0.24297476893467973 1102 9 0.30952232689992343 1102 10-11 0.3886191699659207 1102 12-13 0.42437010325494384 1102 14-15 0.476447148928564 1102 16-17 0.47028206682565354 1102 18-19 0.4477424532912053 1102 20-21 0.4722002958286282 1102 22-23 0.41465374861024884 1102 24-25 0.4833280648766376 1102 26-27 0.4755690906963892 1102 28-29 0.3997437270391515 1102 30-31 0.35454634332480595 1102 32-33 0.4844842235940163 1102 34-35 0.5442580871957929 1102 36-37 0.6014140415980975 1102 38-39 0.5716860067853702 1102 40-41 0.6535157993495417 1102 42-43 0.574534604289596 1102 44-45 0.6210055761148041 1102 46-47 0.47305097007483 1102 48-49 0.5057354535939211 1102 50-51 0.5340943539337388 1102 52-53 0.3828825811407057 1102 54-55 0.3942722191657282 1102 56-57 0.5829797958178986 1102 58-59 0.6459815137393505 1102 60-61 0.6626046379540682 1102 62-63 0.6175382157174525 1102 64-65 0.7174204312108543 1102 66-67 0.751346014496896 1102 68-69 0.6211878959574157 1102 70-71 0.5909776971764806 1102 72-73 0.33805281369412654 1102 74-75 0.3571608920697642 1102 76-77 0.5307175056053453 1102 78-79 0.594795952194886 1102 80-81 0.8101920601871768 1102 82-83 0.5835098731206436 1102 84-85 0.3569014052526853 1102 86-87 0.26908092203662193 1102 88-89 0.4552757028603125 1102 90-91 0.4027772474971698 1102 92-93 0.515275948634212 1102 94-95 0.6227569325245312 1102 96-97 0.511478663780359 1102 98-99 0.3903179521013804 1102 100-101 0.09146305929056808 1103 1 0.23020174115950098 1103 2 0.2551563926646665 1103 3 0.2834642824662552 1103 4 0.14691340610923476 1103 5 0.14151674277538007 1103 6 0.16386046332163318 1103 7 0.22683937847517655 1103 8 0.1975516080820583 1103 9 0.3201111680367674 1103 10-11 0.29626004573738385 1103 12-13 0.2838360033349119 1103 14-15 0.4067506839736481 1103 16-17 0.3490727281658721 1103 18-19 0.32335356325909714 1103 20-21 0.26195633587614964 1103 22-23 0.26200040376880196 1103 24-25 0.35401120994765733 1103 26-27 0.2252336637189103 1103 28-29 0.3600001437666194 1103 30-31 0.4398098296765127 1103 32-33 0.44111646297012896 1103 34-35 0.3627951661292954 1103 36-37 0.41801213452732355 1103 38-39 0.3379342681754949 1103 40-41 0.47076921570906194 1103 42-43 0.432815201785111 1103 44-45 0.45868842701180057 1103 46-47 0.5418956504624575 1103 48-49 0.6087648247927504 1103 50-51 0.5061018127270458 1103 52-53 0.6901617768769555 1103 54-55 0.5834831248554551 1103 56-57 0.5765830315509461 1103 58-59 0.5659732980918051 1103 60-61 0.6744049894357289 1103 62-63 0.585035480727349 1103 64-65 0.6264832559363356 1103 66-67 0.6374067732743214 1103 68-69 0.5942274875356759 1103 70-71 0.3802955822969345 1103 72-73 0.3385400191949337 1103 74-75 0.382698769142813 1103 76-77 0.274306472668858 1103 78-79 0.38165380220858935 1103 80-81 0.4338141305640093 1103 82-83 0.4528454216244775 1103 84-85 0.407041509998308 1103 86-87 0.43724382533360995 1103 88-89 0.4665699533694898 1103 90-91 0.5189583846654813 1103 92-93 0.46062946802581806 1103 94-95 0.5572050469182344 1103 96-97 0.490375139761948 1103 98-99 0.5037808591904032 1103 100-101 0.1101122427279364 1104 1 0.30588895995218834 1104 2 0.2620245704504498 1104 3 0.2665512829358363 1104 4 0.1878207632793334 1104 5 0.07353230089824336 1104 6 0.14744814980084442 1104 7 0.18632899465875852 1104 8 0.21305083391055035 1104 9 0.3245688530894668 1104 10-11 0.26018059229205903 1104 12-13 0.3300077267588506 1104 14-15 0.4991871905836476 1104 16-17 0.5466529294565774 1104 18-19 0.45391977681043016 1104 20-21 0.4960668406702595 1104 22-23 0.41042411221256003 1104 24-25 0.37980119243160715 1104 26-27 0.5351309367617674 1104 28-29 0.49660881338548535 1104 30-31 0.48264952382825754 1104 32-33 0.42488984040889477 1104 34-35 0.39143161408714633 1104 36-37 0.44454653388471854 1104 38-39 0.4616791399397542 1104 40-41 0.35744862911419517 1104 42-43 0.6111144611640995 1104 44-45 0.5675497367422437 1104 46-47 0.4480452477034831 1104 48-49 0.5095888322522271 1104 50-51 0.5832167029624529 1104 52-53 0.5962538240019271 1104 54-55 0.6420045521717981 1104 56-57 0.5508299056306782 1104 58-59 0.7760851595992904 1104 60-61 0.5375279770949035 1104 62-63 0.4658946182470274 1104 64-65 0.6805685488817232 1104 66-67 0.6812464925277197 1104 68-69 0.40685018713815424 1104 70-71 0.26827605688947287 1104 72-73 0.2922691136561042 1104 74-75 0.2599815955147271 1104 76-77 0.11899695406333066 1104 78-79 0.12325231178302687 1104 80-81 0.23480601293646686 1104 82-83 0.15597590304344422 1104 84-85 0.208619913766249 1104 86-87 0.27324056579299594 1104 88-89 0.2043692981552958 1104 90-91 0.25613471883329453 1104 92-93 0.24859831014809686 1104 94-95 0.41845257425300986 1104 96-97 0.15320260145285403 1104 98-99 0.2580263741078248 1104 100-101 -0.091851073358729 1105 1 0.23367695678447475 1105 2 0.22128962369076532 1105 3 0.198882041067435 1105 4 0.2413930964212767 1105 5 0.1810696080876042 1105 6 0.19424060825322442 1105 7 0.1844311156760483 1105 8 0.16350759699543005 1105 9 0.2633295931247446 1105 10-11 0.2518351393510869 1105 12-13 0.3097410495484141 1105 14-15 0.44772842532812973 1105 16-17 0.3119126901817211 1105 18-19 0.3514002163940475 1105 20-21 0.3676353872227338 1105 22-23 0.38157984716504956 1105 24-25 0.41490840081895186 1105 26-27 0.37463982619312475 1105 28-29 0.3211366644157323 1105 30-31 0.33847209277249135 1105 32-33 0.31487279846416527 1105 34-35 0.20580340582931456 1105 36-37 0.35377451201326693 1105 38-39 0.3565527464713867 1105 40-41 0.2693300850257643 1105 42-43 0.40787992985196553 1105 44-45 0.45790659758622354 1105 46-47 0.21210806107986713 1105 48-49 0.10910513670593502 1105 50-51 0.07940965628448282 1105 52-53 0.07332117693064077 1105 54-55 0.16680361881875427 1105 56-57 0.27080874932269694 1105 58-59 0.3168088176464252 1105 60-61 0.18840058884455857 1105 62-63 0.20882639431105332 1105 64-65 0.15320470966508992 1105 66-67 0.014909891771086592 1105 68-69 -0.05442230863001285 1105 70-71 -0.1305714056715388 1105 72-73 0.15901216536757445 1105 74-75 0.11197843696733756 1105 76-77 0.11098815688997732 1105 78-79 0.23669533472833848 1105 80-81 0.37480885517069495 1105 82-83 0.27059730713007113 1105 84-85 0.14358363516769757 1105 86-87 0.129484713712948 1105 88-89 0.0020140830566113266 1105 90-91 0.11033475023539552 1105 92-93 0.1690163248704124 1105 94-95 0.06613695560291433 1105 96-97 -0.07116245144364086 1105 98-99 -0.24345336408572749 1105 100-101 -0.39285576877551165 1106 1 0.0957159888806558 1106 2 0.10535585661823887 1106 3 0.14030863903595758 1106 4 0.10387890278069278 1106 5 -0.06848101432776588 1106 6 0.007720163317905815 1106 7 0.07422332631687567 1106 8 0.030378504380330185 1106 9 -0.01874426808831231 1106 10-11 0.07832338064685729 1106 12-13 -0.022994830756402962 1106 14-15 0.09150022814480963 1106 16-17 -0.15913881902462634 1106 18-19 0.15060221530175255 1106 20-21 0.1634249302899775 1106 22-23 0.19201202067254286 1106 24-25 0.061073376065237994 1106 26-27 0.01618052718448837 1106 28-29 0.17585487980014136 1106 30-31 0.09522488691180087 1106 32-33 0.11387810813201327 1106 34-35 0.048198937865123526 1106 36-37 -0.31558361971035964 1106 38-39 -0.12027985331613422 1106 40-41 -0.0400189406119722 1106 42-43 0.05087993095975918 1106 44-45 -0.002279368185085673 1106 46-47 -0.2726838892288228 1106 48-49 -0.08245893878817157 1106 50-51 -0.26493061222318914 1106 52-53 -0.34092270221764664 1106 54-55 -0.5484755513974804 1106 56-57 -0.20133792734367972 1106 58-59 -0.249779767841666 1106 60-61 -0.5674172251852383 1106 62-63 -0.41765741579936133 1106 64-65 -0.8140329890874263 1106 66-67 -0.6346395120683148 1106 68-69 -1.3727335174241446 1106 70-71 -1.0470229013627623 1106 72-73 -0.6658323277214357 1106 74-75 -0.5093163997047085 1106 76-77 -0.6736837082956377 1106 78-79 -0.6847477804835869 1106 80-81 -1.2888107807409632 1106 82-83 -0.7413434634309048 1106 84-85 -0.6900794152824545 1106 86-87 -0.5516356671917464 1106 88-89 -0.5021271366050009 1106 90-91 -0.5084844939594717 1106 92-93 -0.6368368278625809 1106 94-95 -0.49702399723487645 1106 96-97 -0.6137033817488522 1106 98-99 -0.7302032890325272 1106 100-101 -0.8986139279724945 1107 1 0.11026520038782905 1107 2 0.1578144369374641 1107 3 0.113041077101947 1107 4 0.08780920514356438 1107 5 0.011922483747952128 1107 6 0.13055543902172673 1107 7 0.1626265605153634 1107 8 0.12425165817074912 1107 9 0.15324590886903167 1107 10-11 0.10577826349281594 1107 12-13 0.14877615785749754 1107 14-15 0.2868402946035644 1107 16-17 0.26014672273767303 1107 18-19 0.18172898170976026 1107 20-21 0.29170166302171197 1107 22-23 0.2634975856304962 1107 24-25 0.2692281400600436 1107 26-27 0.21601741275221542 1107 28-29 0.23311964335869817 1107 30-31 0.32399165455407086 1107 32-33 0.34757730511530127 1107 34-35 0.18494948131389322 1107 36-37 0.025241805286256636 1107 38-39 0.08412966286283563 1107 40-41 0.2412203460257345 1107 42-43 0.2665421617225192 1107 44-45 0.2651482863613168 1107 46-47 0.317497590207374 1107 48-49 0.2596127741048875 1107 50-51 0.20995859658136595 1107 52-53 0.33630953421634757 1107 54-55 0.2679890576652113 1107 56-57 0.282026635008485 1107 58-59 0.13873141129585775 1107 60-61 0.21787997186401498 1107 62-63 0.2531734037841673 1107 64-65 0.2984570756156941 1107 66-67 0.17998583014746572 1107 68-69 0.2681334043947814 1107 70-71 0.09107926806982292 1107 72-73 0.1370194083885643 1107 74-75 0.11656527476655043 1107 76-77 0.2088452782987602 1107 78-79 0.3942805235412834 1107 80-81 0.4085474145090373 1107 82-83 0.2640907050525776 1107 84-85 0.3999618793758408 1107 86-87 0.5378404726674404 1107 88-89 0.28928163795127304 1107 90-91 0.2620671796924938 1107 92-93 0.13652402105600459 1107 94-95 -0.14352198463899413 1107 96-97 -0.003933061340184452 1107 98-99 -0.012721635416081511 1107 100-101 -0.2506605193277487 1108 1 0.17202608642267947 1108 2 0.1355198616160891 1108 3 0.18011240002124396 1108 4 0.12873059480408955 1108 5 0.09105111994213644 1108 6 0.1068087911147586 1108 7 0.06857554857409554 1108 8 0.06390135693332866 1108 9 0.12385030777711847 1108 10-11 0.16163886452459764 1108 12-13 0.16739244993153335 1108 14-15 0.2595287032763167 1108 16-17 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66-67 -0.8753402604552143 2316 68-69 -0.8161668903607335 2316 70-71 -0.8806886123303208 2316 72-73 -0.6592649366080998 2316 74-75 -0.46792471142179437 2316 76-77 -0.6162797302697136 2316 78-79 -0.6972657834098968 2316 80-81 -0.8593132963753334 2316 82-83 -0.6370629214902941 2316 84-85 -0.713672130830485 2316 86-87 -0.544622978232649 2316 88-89 -0.4503190602152891 2316 90-91 -0.4837988049965567 2316 92-93 -0.46397697945008076 2316 94-95 -0.24557989512724276 2316 96-97 -0.1584176366282648 2316 98-99 -0.18677469140795822 2316 100-101 -0.10832877499059634 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 14.0 4 57.0 5 119.0 6 268.0 7 637.0 8 1346.0 9 2465.0 10 4107.0 11 6473.0 12 9007.0 13 12772.0 14 16191.0 15 21225.0 16 26568.0 17 33589.0 18 30971.0 19 25318.0 20 24110.0 21 25595.0 22 27715.0 23 31539.0 24 36573.0 25 43258.0 26 51865.0 27 62666.0 28 75926.0 29 90871.0 30 108987.0 31 129925.0 32 155899.0 33 194288.0 34 248551.0 35 327872.0 36 433621.0 37 496467.0 38 256107.0 39 12529.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.28260629574076 18.85740878949877 14.556330677440958 23.303654237319506 2 16.31510773619241 20.625018394468004 38.43410415385943 24.625769715480157 3 19.246305283577524 24.01528490293571 29.9942917940987 26.74411801938807 4 13.398761723697536 16.634462580680985 35.424108972545326 34.542666723076145 5 14.037874612709162 37.31452548229826 33.506521173884074 15.141078731108509 6 33.345507645688514 35.35684727396764 16.62309254416466 14.674552536179192 7 29.35039411719599 32.89407753307226 19.668510551658304 18.08701779807344 8 27.40003622546518 34.432469915676634 19.1795328765928 18.98796098226539 9 27.153894766345747 16.068671054266805 19.39047349591604 37.3869606834714 10-11 25.661379158987486 26.2651809818952 26.66534677289277 21.40809308622454 12-13 26.408380642380617 23.47115977016661 27.388153876256982 22.732305711195785 14-15 22.869952563149976 24.99414971958317 25.63374732605827 26.502150391208584 16-17 22.517398800064516 28.376950423996593 26.35619085267341 22.749459923265476 18-19 22.586858485352483 26.22313828873018 29.095014503407047 22.09498872251029 20-21 23.154517474159608 25.521038533847012 29.17184488097158 22.152599111021797 22-23 22.880430184009498 25.543729028232065 29.146989452307988 22.42885133545045 24-25 22.704777505535464 25.833790283957214 29.2193068827506 22.242125327756717 26-27 22.235058724518854 26.26859075368559 29.00030311774951 22.496047404046042 28-29 22.324835332785103 26.41026463098943 29.049418673830672 22.215481362394794 30-31 22.357259768315675 25.998778382123128 28.924067326481346 22.71989452307985 32-33 22.183106505511823 26.087292794305462 28.898666532694143 22.83093416748857 34-35 22.88964822153855 26.225542291658883 28.638976110771917 22.245833376030642 36-37 23.14076902876772 25.71183888634293 28.631033998938687 22.516358085950664 38-39 22.47036642472373 25.993573015390847 28.71236533460877 22.82369522527665 40-41 22.71880859376614 25.834234749403578 28.811560382205926 22.63539627462436 42-43 22.7297734528888 25.91671650715232 28.42910666734314 22.924403372615735 44-45 22.524752966125224 26.14609307035937 28.63254487859181 22.696609084923598 46-47 22.850534259506542 26.51512347066398 27.90821736336786 22.726124906461617 48-49 22.8173991966937 26.348456583647774 27.861712591918813 22.972431627739716 50-51 22.992824978119287 26.635252533799413 27.609208539690684 22.762713948390612 52-53 23.260946304341505 26.445349787274548 27.503539915438658 22.79016399294529 54-55 22.686114937848206 26.574386480762165 27.6628856888259 23.07661289256373 56-57 22.733974858998327 26.784781734961804 27.57886640735932 22.902376998680545 58-59 22.78434676496019 26.287937581143723 27.58118007758067 23.34653557631542 60-61 22.47702525470204 26.586053989164093 27.913852802978422 23.023067953155447 62-63 22.532222815696997 26.69058230452131 27.817736331497382 22.959458548284314 64-65 22.209102243070227 26.581542332232466 27.66761218656379 23.541743238133517 66-67 22.181696248332297 26.722325887553254 27.72979755871313 23.366180305401315 68-69 22.533381964839553 26.317004594487443 27.853441783575366 23.29617165709764 70-71 22.3938433865935 26.575209440969072 27.436605817660553 23.59434135477688 72-73 22.480152424619373 26.722201371643088 27.21712856621337 23.580517637524167 74-75 22.332619180458344 27.089260736090875 27.00193621145095 23.57618387199983 76-77 22.399963510115366 26.792053269943178 27.313504959186858 23.4944782607546 78-79 22.475075161229764 26.883872925298864 27.247284412208778 23.3937675012626 80-81 22.47233645638253 27.13621919561376 27.074460032880925 23.31698431512278 82-83 22.503030100708088 27.02289589377146 27.019954208873507 23.454119796646943 84-85 22.429413200618576 26.936301775455977 26.945061134541938 23.68922388938351 86-87 22.600545311455207 26.917506190035322 26.77417105089629 23.70777744761318 88-89 22.427865475147357 27.05507829633055 26.603945208268538 23.91311102025356 90-91 22.273848239781238 27.07066623785157 26.854132242741517 23.801353279625676 92-93 22.3577128283709 26.64430867839863 26.951401866687647 24.04657662654282 94-95 22.10317093723718 26.588720154249216 27.04981848415804 24.258290424355565 96-97 22.287519137357975 26.396564398035892 26.984319265336985 24.331597199269144 98-99 22.112934284450738 26.6742059313289 26.634476882122392 24.57838290209797 100-101 22.081222384693287 26.41490849764799 26.9227260588016 24.581143058857126 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1278.0 1 888.0 2 721.0 3 942.0 4 763.5 5 751.5 6 1468.0 7 3135.0 8 4668.0 9 5033.5 10 4134.0 11 3208.0 12 3177.5 13 3358.0 14 3684.0 15 4005.0 16 4181.5 17 4341.5 18 4602.5 19 4972.0 20 5561.0 21 6493.0 22 7799.0 23 9313.5 24 10861.0 25 12956.5 26 15874.5 27 19170.0 28 22277.5 29 25669.5 30 30206.0 31 36975.5 32 46724.0 33 50315.5 34 49316.0 35 52293.5 36 56852.5 37 61951.0 38 66917.5 39 70063.0 40 73525.0 41 81675.5 42 89690.5 43 95472.5 44 100497.0 45 106685.5 46 110221.0 47 112542.5 48 115045.5 49 112979.5 50 112068.5 51 111162.0 52 110158.0 53 113086.5 54 116318.0 55 109724.5 56 98300.0 57 90576.0 58 84176.5 59 73817.0 60 59943.0 61 49581.0 62 42650.0 63 34039.0 64 25675.0 65 20085.0 66 14896.5 67 11813.0 68 8952.5 69 5956.0 70 4411.5 71 3335.5 72 2630.0 73 2179.5 74 1741.5 75 1402.0 76 962.0 77 527.0 78 315.0 79 200.5 80 101.0 81 46.0 82 23.5 83 15.0 84 12.0 85 6.5 86 2.5 87 4.0 88 4.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 7.27153498137165E-4 2 0.049215070851192665 3 8.924156568047024E-4 4 0.0 5 0.0850769592820483 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.652621586675375E-4 26-27 0.008891104136313516 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010742040313389937 36-37 1.4873594280078372E-4 38-39 0.004197658830155452 40-41 0.005404072588428476 42-43 3.470505332018287E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 4.957864760026125E-5 68-69 7.436797140039187E-4 70-71 4.957864760026125E-5 72-73 1.652621586675375E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.001123782678939255 82-83 6.279962029366424E-4 84-85 0.0053049152932279535 86-87 0.0022806177896120172 88-89 9.91572952005225E-5 90-91 0.003470505332018287 92-93 9.91572952005225E-4 94-95 8.263107933376875E-5 96-97 0.0 98-99 6.6104863467015E-5 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3025496.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.306751799150774 #Duplication Level Percentage of deduplicated Percentage of total 1 69.61485387874495 29.45178344582211 2 14.786974507907328 12.511777207328098 3 5.856639024515992 7.433261207622557 4 3.011079833424801 5.095560286405251 5 1.7186955608115169 3.6356213254777536 6 1.0936231019571732 2.7760584681791682 7 0.7356164634323631 2.1785080196461437 8 0.5050708197395836 1.709432464937295 9 0.3953913830401111 1.505495259522084 >10 2.017613442713526 15.5058336009371 >50 0.1568847941185216 4.564062206303754 >100 0.09646155675042795 7.656094169465772 >500 0.006719890313893022 1.9420441968374624 >1k 0.00414132775146972 2.99423962362374 >5k 7.813825946169283E-5 0.26115629997601514 >10k+ 1.5627651892338566E-4 0.779072217915791 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12981 0.42905361633266087 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10586 0.3498930423309104 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7900 0.2611142106947092 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3713 0.12272367902651334 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3463 0.11446057109313647 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.3220972693403E-4 2 0.0 0.0 0.0 0.0 1.3220972693403E-4 3 0.0 0.0 0.0 0.0 1.3220972693403E-4 4 0.0 0.0 0.0 0.0 1.3220972693403E-4 5 0.0 0.0 0.0 0.0 1.3220972693403E-4 6 0.0 0.0 0.0 0.0 1.3220972693403E-4 7 3.30524317335075E-5 0.0 0.0 0.0 1.3220972693403E-4 8 3.30524317335075E-5 0.0 0.0 0.0 1.3220972693403E-4 9 3.30524317335075E-5 0.0 0.0 0.0 1.3220972693403E-4 10-11 3.30524317335075E-5 0.0 0.0 0.0 1.4873594280078375E-4 12-13 3.30524317335075E-5 0.0 0.0 3.30524317335075E-5 4.2968161253559745E-4 14-15 6.6104863467015E-5 0.0 0.0 3.30524317335075E-5 4.792602601358587E-4 16-17 6.6104863467015E-5 0.0 0.0 6.6104863467015E-5 4.957864760026124E-4 18-19 9.91572952005225E-5 0.0 0.0 8.263107933376875E-5 5.94943771203135E-4 20-21 1.3220972693403E-4 0.0 0.0 1.4873594280078375E-4 6.445224188033963E-4 22-23 1.3220972693403E-4 0.0 0.0 4.6273404426910495E-4 6.6104863467015E-4 24-25 1.652621586675375E-4 0.0 0.0 9.419943044049637E-4 6.775748505369037E-4 26-27 1.652621586675375E-4 0.0 0.0 0.001569990507341606 7.436797140039186E-4 28-29 1.652621586675375E-4 0.0 0.0 0.0051231269186936615 7.932583616041799E-4 30-31 1.9831459040104497E-4 0.0 0.0 0.01649316343502024 7.932583616041799E-4 32-33 2.1484080626779872E-4 0.0 0.0 0.040687543463947734 8.924156568047024E-4 34-35 2.478932380013062E-4 0.0 0.0 0.07709479701840624 8.924156568047024E-4 36-37 2.974718856015675E-4 0.0 0.0 0.14080335918474193 8.924156568047024E-4 38-39 3.30524317335075E-4 0.0 0.0 0.23384595451456552 9.254680885382099E-4 40-41 3.30524317335075E-4 0.0 0.0 0.33351556240695746 9.254680885382099E-4 42-43 3.6357674906858245E-4 0.0 0.0 0.43758114371990575 9.419943044049637E-4 44-45 4.6273404426910495E-4 0.0 0.0 0.565080899131911 9.91572952005225E-4 46-47 5.618913394696274E-4 0.0 0.0 0.720576064222197 0.001041151599605486 48-49 5.94943771203135E-4 0.0 0.0 0.8832931856462544 0.0010907302472057475 50-51 5.94943771203135E-4 0.0 0.0 1.059545277865183 0.0010907302472057475 52-53 6.114699870698887E-4 0.0 0.0 1.233582857157967 0.0012890448376067923 54-55 6.279962029366424E-4 0.0 0.0 1.4193871021478792 0.0013551497010738075 56-57 6.279962029366424E-4 0.0 0.0 1.6428545930981233 0.0013551497010738075 58-59 6.6104863467015E-4 0.0 0.0 1.876353497079487 0.0013716759169405612 60-61 6.6104863467015E-4 0.0 0.0 2.138178335056467 0.001437780780407576 62-63 6.6104863467015E-4 0.0 0.0 2.3938058420834136 0.0014708332121410837 64-65 6.6104863467015E-4 0.0 0.0 2.6646705201395076 0.001520411859741345 66-67 6.941010664036574E-4 0.0 0.0 2.978850409982363 0.001520411859741345 68-69 7.271534981371649E-4 0.0 0.0 3.3252564207653883 0.001520411859741345 70-71 7.602059298706723E-4 0.0 0.0 3.688188647415168 0.001520411859741345 72-73 7.932583616041799E-4 0.0 0.0 4.066308466446493 0.0016360953708086212 74-75 7.932583616041799E-4 0.0 0.0 4.448378051069973 0.0017683050977426512 76-77 7.932583616041799E-4 0.0 0.0 4.864822164696301 0.001784831313609405 78-79 7.932583616041799E-4 0.0 0.0 5.317012483242417 0.0018178837453429123 80-81 9.254680885382099E-4 0.0 0.0 5.796024850140275 0.0018674623929431735 82-83 9.254680885382099E-4 0.0 0.0 6.2957610917350415 0.0020823031992109726 84-85 9.419943044049637E-4 0.0 0.0 6.798686892992091 0.0020823031992109726 86-87 9.585205202717174E-4 0.0 0.0 7.3222704640825835 0.0021318818468112335 88-89 9.585205202717174E-4 0.0 0.0 7.885996213513421 0.002181460494411495 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6650 0.0 50.635853 1 GTATCAA 11085 0.0 44.878933 1 TATCAAC 14795 0.0 33.52808 2 ATCAACG 15150 0.0 32.6326 3 TCAACGC 15480 0.0 31.998362 4 CAACGCA 15730 0.0 31.489807 5 AACGCAG 16240 0.0 30.53217 6 ACGCAGA 18800 0.0 26.12197 7 CGCAGAG 18985 0.0 25.842405 8 GTACATG 13545 0.0 25.492014 1 TACATGG 13770 0.0 24.556982 2 GTGGTAT 2685 0.0 24.09024 1 GCAGAGT 20710 0.0 23.208317 9 ACATGGG 14300 0.0 22.771177 3 TGGTATC 2880 0.0 21.63307 2 AGTACTT 11780 0.0 21.630554 12-13 GAGTACT 11445 0.0 21.267736 12-13 AGAGTAC 19025 0.0 20.945005 10-11 GTGTAAG 1115 0.0 20.474483 1 CAGAGTA 19655 0.0 19.98369 10-11 >>END_MODULE