FastQCFastQC Report
Thu 26 May 2016
SRR938493_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938493_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3025288
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135970.44944481318803364No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102140.3376207488344911No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72120.2383905267862101No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA34980.1156253553380703No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA34350.11354290897263335No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA31280.10339511477915492No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67600.049.2049061
GTATCAA112650.044.967811
TATCAAC146600.034.3943332
ATCAACG147900.034.0598833
TCAACGC150750.033.384444
CAACGCA152400.033.0541765
AACGCAG157200.032.007786
GTACATG134400.027.549991
ACGCAGA181100.027.4891667
CGCAGAG181250.027.4139988
TACATGG135900.026.6465472
ACATGGG140650.024.766793
GCAGAGT194500.024.6179839
GTGGTAT26450.023.7819771
GAGTACT112300.022.75694812-13
AGTACTT115350.022.05227512-13
GTACTTT119250.021.49182714-15
GAGTACA104250.021.1643331
TAGGACG2257.124618E-821.121414
CAGAGTA186300.020.99741710-11
Produced by FastQC (version 0.11.5)