##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938493_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3025288 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.02547988819577 31.0 31.0 34.0 28.0 34.0 2 31.159846269181646 31.0 31.0 34.0 27.0 34.0 3 31.21205683558061 31.0 31.0 34.0 27.0 34.0 4 34.894894304277805 37.0 35.0 37.0 32.0 37.0 5 34.900343372267365 37.0 35.0 37.0 32.0 37.0 6 34.90513696547238 37.0 35.0 37.0 32.0 37.0 7 34.81222415849334 37.0 35.0 37.0 32.0 37.0 8 34.77047044777225 37.0 35.0 37.0 31.0 37.0 9 36.2385191095856 39.0 35.0 39.0 32.0 39.0 10-11 36.08917795594998 39.0 35.0 39.0 30.0 39.0 12-13 35.91257460446741 38.0 35.0 39.0 30.0 39.0 14-15 36.86760450575284 39.0 36.0 41.0 30.5 41.0 16-17 36.69753920949014 39.0 36.0 40.5 30.0 41.0 18-19 36.573024948368555 39.0 36.0 40.0 30.0 41.0 20-21 36.56248826558 39.0 36.0 40.5 30.0 41.0 22-23 36.65229657473933 39.0 36.0 41.0 30.0 41.0 24-25 36.557201826735174 39.0 36.0 41.0 30.0 41.0 26-27 36.350043202498405 39.0 35.0 41.0 29.5 41.0 28-29 36.152363675788884 39.0 35.0 40.0 29.0 41.0 30-31 35.868124125703076 38.0 35.0 40.0 27.5 41.0 32-33 35.5445623689381 38.0 34.0 40.0 27.0 41.0 34-35 35.257382768186034 38.0 34.0 40.0 26.0 41.0 36-37 34.95060007510028 38.0 34.0 40.0 25.0 41.0 38-39 34.612775709287845 38.0 33.0 40.0 24.5 41.0 40-41 34.46518298423158 38.0 33.0 40.0 24.0 41.0 42-43 34.20801292306716 38.0 33.0 40.0 23.5 41.0 44-45 33.86470081526123 38.0 33.0 40.0 22.5 41.0 46-47 33.41809457479751 37.5 32.0 40.0 20.0 41.0 48-49 32.980964456937656 37.0 31.5 40.0 17.5 41.0 50-51 32.162226703705564 36.0 30.5 39.0 14.5 40.5 52-53 32.16066189400811 36.5 31.0 39.0 13.5 40.0 54-55 32.21278155997049 36.5 31.0 39.0 10.5 41.0 56-57 31.858269526735967 36.0 30.5 39.0 8.0 41.0 58-59 31.394720271260127 35.5 30.0 39.0 7.0 40.5 60-61 30.886737064372053 35.0 29.0 39.0 2.0 40.0 62-63 30.280940690605323 35.0 28.0 38.5 2.0 40.0 64-65 29.757613324747922 34.5 28.0 38.0 2.0 40.0 66-67 29.181653283918756 34.0 26.5 37.5 2.0 40.0 68-69 28.53917709652767 34.0 26.0 37.0 2.0 39.0 70-71 27.87269212055183 33.5 25.5 36.0 2.0 39.0 72-73 27.22312140199545 33.0 24.0 36.0 2.0 39.0 74-75 26.496453230237915 32.0 22.0 35.0 2.0 38.0 76-77 25.738622901356827 32.0 19.5 35.0 2.0 37.0 78-79 24.924502394482772 31.0 16.5 35.0 2.0 36.5 80-81 24.13987974037513 31.0 8.5 34.5 2.0 36.0 82-83 23.381261883166164 30.0 4.5 34.0 2.0 35.5 84-85 22.618483926158437 29.5 2.0 34.0 2.0 35.0 86-87 21.858124747131512 29.0 2.0 34.0 2.0 35.0 88-89 21.04950355139742 29.0 2.0 34.0 2.0 35.0 90-91 20.200104585084134 27.0 2.0 34.0 2.0 35.0 92-93 19.319537015979968 25.5 2.0 33.5 2.0 35.0 94-95 18.39818523062928 24.5 2.0 33.0 2.0 35.0 96-97 17.31415703232221 21.0 2.0 32.5 2.0 35.0 98-99 16.05930278373497 4.5 2.0 32.0 2.0 34.0 100-101 13.384454802319647 2.0 2.0 28.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5578271069253731 1101 2 0.5648835232620222 1101 3 0.5399007894997965 1101 4 0.44856084062876533 1101 5 0.5566042197646439 1101 6 0.4776438873316948 1101 7 0.5518959416939566 1101 8 0.5843484506474184 1101 9 0.6242226338664594 1101 10-11 0.7688765674037512 1101 12-13 0.7957057332167494 1101 14-15 0.9285856957968619 1101 16-17 1.0110041167086337 1101 18-19 0.9970250264352671 1101 20-21 1.07427846542231 1101 22-23 0.9487100751072219 1101 24-25 1.1076553795527175 1101 26-27 0.9936357590324434 1101 28-29 1.0796102939919265 1101 30-31 0.9571614952250584 1101 32-33 1.0834271685299868 1101 34-35 1.0240872207314382 1101 36-37 1.122643762269938 1101 38-39 1.1147453337413111 1101 40-41 1.193300740879728 1101 42-43 1.2571373234896228 1101 44-45 1.3370470305543734 1101 46-47 1.539655710004645 1101 48-49 1.5900469880274173 1101 50-51 1.6760755164529115 1101 52-53 1.6704202105214065 1101 54-55 1.8115252940281223 1101 56-57 1.9082321083244551 1101 58-59 2.1390025010765186 1101 60-61 2.248416494999965 1101 62-63 2.2293758592858985 1101 64-65 2.2602071203577374 1101 66-67 2.4651657693308913 1101 68-69 2.6039033947975234 1101 70-71 2.705128942213509 1101 72-73 2.95538337807805 1101 74-75 3.245449214634249 1101 76-77 3.3087667077188634 1101 78-79 3.7319273879445802 1101 80-81 3.971189288744938 1101 82-83 4.039765946825316 1101 84-85 4.283224595316856 1101 86-87 4.428905467989452 1101 88-89 4.759471716015728 1101 90-91 4.9962203737220285 1101 92-93 4.964644979981795 1101 94-95 5.376445910083696 1101 96-97 5.4870151166202135 1101 98-99 5.797301678801702 1101 100-101 5.7293358530755345 1102 1 0.45256405909203323 1102 2 0.5022332903757807 1102 3 0.3515013265113538 1102 4 0.3766764347361402 1102 5 0.4394112064913145 1102 6 0.45026131678619663 1102 7 0.4694459867934455 1102 8 0.4655565314723944 1102 9 0.533863887283772 1102 10-11 0.5138321161746262 1102 12-13 0.6020818392695588 1102 14-15 0.8084265719677433 1102 16-17 0.9442535942655326 1102 18-19 0.7668676173626707 1102 20-21 0.8293968742397837 1102 22-23 0.6694880448076148 1102 24-25 0.47611466384400813 1102 26-27 0.5991125240815265 1102 28-29 0.616063508323947 1102 30-31 0.7224737133532457 1102 32-33 0.8148700873522969 1102 34-35 0.9777903977709812 1102 36-37 1.0036865712363081 1102 38-39 0.9442645885053338 1102 40-41 1.3959989033020221 1102 42-43 1.4493201248999057 1102 44-45 1.537669291997581 1102 46-47 1.5685020273907426 1102 48-49 1.5304672846105873 1102 50-51 1.6922651880421853 1102 52-53 1.659384427874464 1102 54-55 1.9371813278102152 1102 56-57 1.9709643149372909 1102 58-59 2.1132792711443926 1102 60-61 2.1636519673178043 1102 62-63 2.358273619367761 1102 64-65 2.4800269175703527 1102 66-67 2.5159525358716692 1102 68-69 2.5452877191346595 1102 70-71 2.7196454017849874 1102 72-73 2.669338498856856 1102 74-75 2.874842999408603 1102 76-77 3.0558279914441506 1102 78-79 3.4486114809987036 1102 80-81 3.880170291194389 1102 82-83 4.26483938162205 1102 84-85 4.374974873790524 1102 86-87 4.571526996130281 1102 88-89 4.811682776055907 1102 90-91 5.114205311746822 1102 92-93 5.095752754520586 1102 94-95 5.40367317666421 1102 96-97 5.451418444084339 1102 98-99 5.94784370915707 1102 100-101 5.73840336011231 1103 1 0.646700144749051 1103 2 0.6116490516555402 1103 3 0.5891018904174281 1103 4 0.5113604648793881 1103 5 0.3866876950471436 1103 6 0.5425324169937227 1103 7 0.5474159774343903 1103 8 0.5174112127722665 1103 9 0.6764290823603574 1103 10-11 0.6505923996503213 1103 12-13 0.7174830644788912 1103 14-15 0.7358062726617334 1103 16-17 0.8541248383373699 1103 18-19 0.9251883166271995 1103 20-21 0.9954723977496513 1103 22-23 0.8763509366114164 1103 24-25 1.0270242511661039 1103 26-27 0.9841652721391583 1103 28-29 1.1499520187377001 1103 30-31 1.0115333317190505 1103 32-33 1.1678415547025836 1103 34-35 1.2268273207550138 1103 36-37 1.251822045207497 1103 38-39 1.3608312327260137 1103 40-41 1.517304996926704 1103 42-43 1.6406443590784434 1103 44-45 1.3892959693753184 1103 46-47 1.5219129634407054 1103 48-49 1.5769758843094692 1103 50-51 1.565061511101554 1103 52-53 1.737127595960736 1103 54-55 1.8405243352305192 1103 56-57 1.9702134902771604 1103 58-59 2.0984239689702804 1103 60-61 2.3379982445007386 1103 62-63 2.360228126219038 1103 64-65 2.435401483253724 1103 66-67 2.5501783923302703 1103 68-69 2.654859683446862 1103 70-71 2.936761250617206 1103 72-73 3.121388499578803 1103 74-75 3.3731714956984504 1103 76-77 3.4350201723666416 1103 78-79 3.435055026728783 1103 80-81 3.9186813612639213 1103 82-83 4.198504547952023 1103 84-85 4.58631888931107 1103 86-87 4.680182875599293 1103 88-89 5.040403460093923 1103 90-91 5.081062900523307 1103 92-93 5.149205694747334 1103 94-95 5.251951005415805 1103 96-97 5.585474511784572 1103 98-99 5.791996727747858 1103 100-101 5.488313182395265 1104 1 0.6199151375648988 1104 2 0.5813737911593257 1104 3 0.6102413987272861 1104 4 0.5635502169978608 1104 5 0.44864676235189904 1104 6 0.5052823066858778 1104 7 0.4974889503156348 1104 8 0.6228202568840899 1104 9 0.7327200235408213 1104 10-11 0.6688921880855361 1104 12-13 0.7872428762201764 1104 14-15 0.8737278466132352 1104 16-17 0.9799776028216343 1104 18-19 1.1663240914408135 1104 20-21 1.1053107260484225 1104 22-23 1.1124012369881626 1104 24-25 1.2330651612341015 1104 26-27 1.1599931466484321 1104 28-29 1.1460375044881914 1104 30-31 1.2047276206011475 1104 32-33 1.276735195313968 1104 34-35 1.4458110178213914 1104 36-37 1.4459016252457388 1104 38-39 1.5507785112335242 1104 40-41 1.721179347807471 1104 42-43 1.8482239221462464 1104 44-45 1.728625523957895 1104 46-47 1.6401092322071733 1104 48-49 1.7783782081280393 1104 50-51 1.7936221917292343 1104 52-53 2.0185709882380607 1104 54-55 2.0739498746075853 1104 56-57 2.200745570060839 1104 58-59 2.3091260925376957 1104 60-61 2.393234190150082 1104 62-63 2.5302096850102878 1104 64-65 2.6431592359152596 1104 66-67 2.6697888642215695 1104 68-69 2.8781962977314457 1104 70-71 2.9716496417065947 1104 72-73 3.137745172920507 1104 74-75 3.1680356756303425 1104 76-77 3.3871437397788746 1104 78-79 3.6516741761704523 1104 80-81 4.195565362666809 1104 82-83 4.456692784044751 1104 84-85 4.556982659689734 1104 86-87 4.770544798463 1104 88-89 5.1931967941060755 1104 90-91 5.401153635795794 1104 92-93 5.4014655913407985 1104 94-95 5.758447854571244 1104 96-97 6.110302261843966 1104 98-99 6.191209299447959 1104 100-101 5.853409517912125 1105 1 0.6045590243388688 1105 2 0.5957705386381207 1105 3 0.5706374727716756 1105 4 0.5323245663823144 1105 5 0.45667788503940443 1105 6 0.5010789072335271 1105 7 0.5796409906549087 1105 8 0.5235575562451587 1105 9 0.6883004850381838 1105 10-11 0.7172472828115914 1105 12-13 0.7273733054372045 1105 14-15 0.93921412469156 1105 16-17 1.0293769382921525 1105 18-19 1.054399363040801 1105 20-21 0.9990990589365083 1105 22-23 0.9598432993671082 1105 24-25 1.0019216723986446 1105 26-27 1.040033817077422 1105 28-29 1.0529202406354798 1105 30-31 0.9904081650255563 1105 32-33 1.0640311474722992 1105 34-35 1.229521001347294 1105 36-37 1.3737366588618016 1105 38-39 1.4935905033749322 1105 40-41 1.578934765893358 1105 42-43 1.7209357757048735 1105 44-45 1.689801821849663 1105 46-47 1.723443956933906 1105 48-49 1.7650890097932646 1105 50-51 1.639847132734154 1105 52-53 1.8868394269833573 1105 54-55 2.0967276802132346 1105 56-57 2.260528526604844 1105 58-59 2.2466003517598523 1105 60-61 2.584307636241931 1105 62-63 2.7283437568952316 1105 64-65 2.8002548552522875 1105 66-67 2.829026185844935 1105 68-69 2.814999557487898 1105 70-71 3.057492005183075 1105 72-73 3.2575110988093314 1105 74-75 3.344868210303865 1105 76-77 3.528617489751049 1105 78-79 3.8299043655112683 1105 80-81 4.257689338601267 1105 82-83 4.43434422330208 1105 84-85 4.563094007337725 1105 86-87 4.827388436455834 1105 88-89 5.096227457633756 1105 90-91 5.220270407095246 1105 92-93 5.4389086833508635 1105 94-95 5.599600818795736 1105 96-97 5.714695377785997 1105 98-99 5.896916029896097 1105 100-101 5.253428162583644 1106 1 0.30434388036831095 1106 2 0.30344084967225626 1106 3 0.27626865195908934 1106 4 0.31528805054194464 1106 5 0.32329764400599004 1106 6 0.1196354129707018 1106 7 0.23613860918705853 1106 8 0.30769689015581747 1106 9 0.30246796356888694 1106 10-11 0.21427676249695082 1106 12-13 0.1441044701914862 1106 14-15 0.5536871798261487 1106 16-17 0.342290537916881 1106 18-19 0.4817034782894183 1106 20-21 0.5598880554725625 1106 22-23 0.46351112068485634 1106 24-25 0.42646815612527433 1106 26-27 0.5476849590995059 1106 28-29 0.422545024138401 1106 30-31 0.6138662999032363 1106 32-33 0.8138432911541926 1106 34-35 0.7544898798924322 1106 36-37 0.7505928957817716 1106 38-39 0.6663838118111016 1106 40-41 0.6687605308497027 1106 42-43 0.44666058987821344 1106 44-45 0.6239093577520904 1106 46-47 1.0891427012508572 1106 48-49 1.0047936716969943 1106 50-51 0.4556552531786977 1106 52-53 0.8283166587073936 1106 54-55 0.7589890823926382 1106 56-57 0.9119783804693142 1106 58-59 0.21831004722062985 1106 60-61 1.4148141040921232 1106 62-63 1.7463732275598893 1106 64-65 1.7947181680183952 1106 66-67 1.8245686012306628 1106 68-69 1.5134310812664928 1106 70-71 1.9857017998716806 1106 72-73 2.36734299784953 1106 74-75 2.5339055650704125 1106 76-77 2.5457701472526715 1106 78-79 2.906479156230759 1106 80-81 3.4825393692716915 1106 82-83 3.735810991000715 1106 84-85 3.822433375527087 1106 86-87 4.15285277758306 1106 88-89 4.383660478850473 1106 90-91 4.7182105276119515 1106 92-93 5.236572770094025 1106 94-95 5.077662448297069 1106 96-97 5.389674404631634 1106 98-99 5.480892765481002 1106 100-101 4.629473663052723 1107 1 0.4633122992692762 1107 2 0.40342637074335386 1107 3 0.3867416019130978 1107 4 0.45909234652531694 1107 5 0.5189186197154569 1107 6 0.4110183210209897 1107 7 0.39466882756337895 1107 8 0.3380719795844982 1107 9 0.354429858434834 1107 10-11 0.42188096819994314 1107 12-13 0.5055428240224344 1107 14-15 0.665961265950223 1107 16-17 0.7440759176055067 1107 18-19 0.8112571014284455 1107 20-21 0.7799217828598799 1107 22-23 0.7656569831179922 1107 24-25 0.785879459910241 1107 26-27 0.7853440143919883 1107 28-29 0.7987012091961461 1107 30-31 0.8302973290995723 1107 32-33 0.827155601650567 1107 34-35 0.9350791722986642 1107 36-37 1.005998646471646 1107 38-39 1.115309603041979 1107 40-41 1.1263504705492196 1107 42-43 1.1657446698700369 1107 44-45 1.387828505709713 1107 46-47 1.363805427377656 1107 48-49 1.384402229595672 1107 50-51 1.3651836659474128 1107 52-53 1.5925801070772323 1107 54-55 1.5972717962549368 1107 56-57 1.6568181327944558 1107 58-59 1.9710848913592187 1107 60-61 1.9651794841214212 1107 62-63 2.004525290381398 1107 64-65 2.2463302264110716 1107 66-67 2.4475292015100187 1107 68-69 2.655843909597497 1107 70-71 2.641206560884182 1107 72-73 3.0107340055829788 1107 74-75 3.10805918650604 1107 76-77 3.2307293081561568 1107 78-79 3.320382334781506 1107 80-81 3.673880969789522 1107 82-83 3.867346311070218 1107 84-85 4.2155616332860895 1107 86-87 4.473925368118948 1107 88-89 4.610573187943238 1107 90-91 4.837944456000873 1107 92-93 5.181679956752969 1107 94-95 5.094030025716755 1107 96-97 4.9955488041819365 1107 98-99 5.080935563491426 1107 100-101 4.92620291559931 1108 1 0.2994732174784076 1108 2 0.4096452882190533 1108 3 0.34824873038645876 1108 4 0.221932686092849 1108 5 0.20214989943083594 1108 6 0.18118898941187211 1108 7 0.25718555502794516 1108 8 0.23366043248703505 1108 9 0.31468162286066104 1108 10-11 0.3850714988465924 1108 12-13 0.36525327804442753 1108 14-15 0.23716618221639862 1108 16-17 0.5047298247552092 1108 18-19 0.6315482397324104 1108 20-21 0.5445060410289884 1108 22-23 0.630583108844192 1108 24-25 0.621434811733856 1108 26-27 0.7043298073059745 1108 28-29 0.6561152557628276 1108 30-31 0.7314524892007057 1108 32-33 0.7824432094944527 1108 34-35 0.8913798774256279 1108 36-37 0.9644912124003753 1108 38-39 0.9620894454821354 1108 40-41 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0.6086326306282714 2311 46-47 0.378393805193987 2311 48-49 0.5606843102189103 2311 50-51 0.6570907322668162 2311 52-53 0.7071245816805103 2311 54-55 0.7362814412301333 2311 56-57 0.8387910863554708 2311 58-59 0.8492008370367188 2311 60-61 0.861295556435465 2311 62-63 1.0829023476531603 2311 64-65 1.07984126095538 2311 66-67 1.012258500630125 2311 68-69 1.0419611777114817 2311 70-71 1.3969029646350641 2311 72-73 1.3619197070197693 2311 74-75 1.4717311753406648 2311 76-77 1.639365663296914 2311 78-79 1.9237726850765071 2311 80-81 1.8669946497408212 2311 82-83 2.10117693125364 2311 84-85 2.438491688334846 2311 86-87 2.539606014773991 2311 88-89 2.5388703440372815 2311 90-91 2.670932208873463 2311 92-93 2.9675517139800256 2311 94-95 3.040681614242569 2311 96-97 3.0751223765837103 2311 98-99 3.2887469501768862 2311 100-101 3.115133345980487 2312 1 -0.25719166250685177 2312 2 -0.2042383429875727 2312 3 -0.1909622264372679 2312 4 -0.17419691530970027 2312 5 -0.28487628103515306 2312 6 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0.9186249139961369 2312 68-69 1.1841499707795435 2312 70-71 1.1743764197063093 2312 72-73 1.280403637059532 2312 74-75 1.3607371589562263 2312 76-77 1.4243443916031602 2312 78-79 1.5195698538822597 2312 80-81 1.8308740823367629 2312 82-83 2.0306103878608432 2312 84-85 2.268133293728706 2312 86-87 2.561538435271615 2312 88-89 2.4545698206198807 2312 90-91 2.74557208631461 2312 92-93 2.9526547754355583 2312 94-95 3.063517734147922 2312 96-97 3.420032198637685 2312 98-99 3.413291823321323 2312 100-101 3.079903104557024 2313 1 -0.5010720709349492 2313 2 -0.5404291362806077 2313 3 -0.44663403260466694 2313 4 -0.49360066492551624 2313 5 -0.3639272662484174 2313 6 -0.2984522255496671 2313 7 -0.3153890168469644 2313 8 -0.2923479826395621 2313 9 -0.500532660748263 2313 10-11 -0.4684647702016633 2313 12-13 -0.3887229061156816 2313 14-15 -0.2975458465512517 2313 16-17 -0.37571808546378804 2313 18-19 -0.1831045441860084 2313 20-21 -0.19536596356911673 2313 22-23 -0.3750716793775766 2313 24-25 -0.29059911404797134 2313 26-27 -0.2857374694942152 2313 28-29 -0.24681953805919932 2313 30-31 -0.20101390924300944 2313 32-33 -0.047869483935549795 2313 34-35 0.033661098871675676 2313 36-37 0.12233083865675098 2313 38-39 0.09582621855373219 2313 40-41 0.1906994258083401 2313 42-43 0.2516200092632559 2313 44-45 0.39860792962659986 2313 46-47 0.3338340544582863 2313 48-49 0.36773654667748445 2313 50-51 0.5218387243820715 2313 52-53 0.36383054976405305 2313 54-55 0.5442630015435626 2313 56-57 0.7342743067998896 2313 58-59 0.6601490514347716 2313 60-61 0.7706378125360089 2313 62-63 0.8563749834518752 2313 64-65 0.9098705404235794 2313 66-67 0.9555093691407599 2313 68-69 1.198228958588171 2313 70-71 1.3432657923798743 2313 72-73 1.4005898372439383 2313 74-75 1.3708661160302995 2313 76-77 1.5284229098560722 2313 78-79 1.6082106608350983 2313 80-81 1.76354939736596 2313 82-83 1.7980163913440919 2313 84-85 2.0770279595757835 2313 86-87 2.208642343682641 2313 88-89 2.2691480315272337 2313 90-91 2.6812997269252037 2313 92-93 2.622635865146311 2313 94-95 2.8228113089182685 2313 96-97 3.040640145227858 2313 98-99 3.0417061336720437 2313 100-101 2.911831825999501 2314 1 -0.48071603533808727 2314 2 -0.422211691406865 2314 3 -0.466497983079762 2314 4 -0.345238214393504 2314 5 -0.33774622847529656 2314 6 -0.3213873764479587 2314 7 -0.25692948852549335 2314 8 -0.35468878109140434 2314 9 -0.4432222796689729 2314 10-11 -0.46285772850458784 2314 12-13 -0.39245401453344186 2314 14-15 -0.502265645130322 2314 16-17 -0.4448300973963768 2314 18-19 -0.4810863171990789 2314 20-21 -0.5477875513660067 2314 22-23 -0.4617832885352655 2314 24-25 -0.45940029793161585 2314 26-27 -0.3996940548470107 2314 28-29 -0.4519759690651597 2314 30-31 -0.37329718755611196 2314 32-33 -0.1876407844665593 2314 34-35 -0.4492936215965173 2314 36-37 -0.30520098491243886 2314 38-39 -0.1810598426894785 2314 40-41 -0.008212696945932407 2314 42-43 0.06152699939979556 2314 44-45 0.08076835824872575 2314 46-47 0.2586643901375183 2314 48-49 0.23251533855368933 2314 50-51 0.237764989456835 2314 52-53 0.3383416453251513 2314 54-55 0.4432488659192515 2314 56-57 0.47679116441913294 2314 58-59 0.6335757015395629 2314 60-61 0.7590059715567605 2314 62-63 0.8614223505160723 2314 64-65 0.8902227508307767 2314 66-67 1.0991852508595308 2314 68-69 1.2844778620895987 2314 70-71 1.3313473551330723 2314 72-73 1.5296196787454903 2314 74-75 1.481973353590483 2314 76-77 1.642756196874199 2314 78-79 1.874128346436315 2314 80-81 2.1965308606809693 2314 82-83 2.2251857844697973 2314 84-85 2.476365289409248 2314 86-87 2.71921229694334 2314 88-89 2.7194674809008035 2314 90-91 2.707255354725042 2314 92-93 2.9283898460220357 2314 94-95 2.957141632879189 2314 96-97 3.12316338095329 2314 98-99 3.3412065572050764 2314 100-101 3.0102989461087812 2315 1 -0.6373677039051486 2315 2 -0.584173160734899 2315 3 -0.6319336851813908 2315 4 -0.4368050386883269 2315 5 -0.539279739282712 2315 6 -0.5375264031125866 2315 7 -0.5777513268077783 2315 8 -0.6076357851200029 2315 9 -0.720738084860514 2315 10-11 -0.5314736173758163 2315 12-13 -0.5805638455051323 2315 14-15 -0.585322741556709 2315 16-17 -0.5090098705192077 2315 18-19 -0.5481575329688155 2315 20-21 -0.4942942368215668 2315 22-23 -0.5374358409159328 2315 24-25 -0.5818946796880837 2315 26-27 -0.6392081674379568 2315 28-29 -0.5545401956048792 2315 30-31 -0.5921261687062511 2315 32-33 -0.41494902434033776 2315 34-35 -0.4566639034848734 2315 36-37 -0.2631989145111291 2315 38-39 -0.16255452638641543 2315 40-41 -0.3132617718593451 2315 42-43 -0.26643724824740644 2315 44-45 -0.21609435353683182 2315 46-47 -0.13230884019704803 2315 48-49 -0.11447753608467082 2315 50-51 0.0809819826199103 2315 52-53 0.274927727286574 2315 54-55 0.28590195065547874 2315 56-57 0.5388456834309636 2315 58-59 0.7073095731083328 2315 60-61 0.7153609848606521 2315 62-63 0.8838083771578589 2315 64-65 0.8526087374583646 2315 66-67 1.0935338705614264 2315 68-69 1.357399653838705 2315 70-71 1.4935921750033252 2315 72-73 1.628816126951861 2315 74-75 1.6237021436894992 2315 76-77 1.6879929567178173 2315 78-79 1.9712763177748087 2315 80-81 2.1667352048557618 2315 82-83 2.418082105514383 2315 84-85 2.5901184444871106 2315 86-87 2.7294169595644604 2315 88-89 2.8055566948902317 2315 90-91 2.8289722939523116 2315 92-93 2.8581061153449348 2315 94-95 2.8012588621427064 2315 96-97 3.180903035574069 2315 98-99 3.293038007064535 2315 100-101 3.027983221079017 2316 1 -0.8707857518995361 2316 2 -0.7996180229905079 2316 3 -0.8793797435671564 2316 4 -0.6938285570250287 2316 5 -0.6455359680749311 2316 6 -0.5852722685583842 2316 7 -0.5542624555832134 2316 8 -0.6739995410293034 2316 9 -0.7486407167677953 2316 10-11 -0.6856347831177985 2316 12-13 -0.7082796999375702 2316 14-15 -1.220167011438285 2316 16-17 -0.9779718588700632 2316 18-19 -0.9268030233274516 2316 20-21 -0.7516635115312695 2316 22-23 -0.9776283650014364 2316 24-25 -1.159806631106072 2316 26-27 -0.862184364548888 2316 28-29 -0.8675072971427653 2316 30-31 -0.6432333720397949 2316 32-33 -0.602673772296825 2316 34-35 -0.5739785273041349 2316 36-37 -0.5545764897310264 2316 38-39 -0.6723487680905791 2316 40-41 -0.7192477769344876 2316 42-43 -0.6743786247366117 2316 44-45 -0.6220961436910883 2316 46-47 -0.2886463684607605 2316 48-49 -0.2858262115607815 2316 50-51 -0.2502665556817689 2316 52-53 -0.11041299385666292 2316 54-55 -0.12589902500553052 2316 56-57 0.06057369750973862 2316 58-59 0.23302338280475965 2316 60-61 0.02716849343973493 2316 62-63 0.2962400824868858 2316 64-65 0.41793522424864804 2316 66-67 0.6399226822320436 2316 68-69 0.8249523503258978 2316 70-71 0.7036233023374727 2316 72-73 1.0479272358928178 2316 74-75 1.258171486371868 2316 76-77 1.2307625654482202 2316 78-79 1.4638678539753442 2316 80-81 1.760784810919116 2316 82-83 1.9538090433454158 2316 84-85 2.0387337184159016 2316 86-87 2.452073666674046 2316 88-89 2.4257081710350654 2316 90-91 2.470190578781814 2316 92-93 2.7998818618192196 2316 94-95 3.0117666146791358 2316 96-97 2.9589206249508706 2316 98-99 3.0789864832192997 2316 100-101 2.831104726788668 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8349.0 3 3128.0 4 3517.0 5 4294.0 6 5143.0 7 6571.0 8 8416.0 9 10844.0 10 13999.0 11 17835.0 12 22209.0 13 26099.0 14 29858.0 15 35653.0 16 41125.0 17 47662.0 18 46329.0 19 43588.0 20 46779.0 21 50689.0 22 55915.0 23 62948.0 24 70279.0 25 78349.0 26 86934.0 27 96767.0 28 108384.0 29 119792.0 30 134071.0 31 150663.0 32 170344.0 33 194653.0 34 225753.0 35 263383.0 36 293555.0 37 285215.0 38 145951.0 39 10245.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.60072727658525 18.029337732711266 13.845077090787564 23.524857899915922 2 15.852986330884583 19.854258706997374 39.24959568661853 25.043159275499516 3 19.124323462759573 23.984776651359326 29.469031318709842 27.42186856717126 4 13.016069646377831 15.961609305937047 35.70428464378851 35.318036403896606 5 14.0680176442242 37.1597985010281 33.35766464398177 15.414519210765926 6 33.279655189862865 34.913051660334766 16.962822511217674 14.844470638584697 7 29.428429157235048 32.72184054897464 19.746482114480813 18.103248179309496 8 27.395228786524108 34.01361106555426 19.34534358979785 19.24581655812379 9 27.428644177368177 16.04577222021061 19.406965035936253 37.11861856648496 10-11 25.58275486039608 26.070943696182507 26.70881798420946 21.637483459211953 12-13 26.471035592392017 23.43332237762284 27.304286813005863 22.791355216979277 14-15 22.998478413105882 24.876094157198906 25.624902049083577 26.500525380611638 16-17 22.52333446789743 28.249174740999518 26.305119275527648 22.922371515575403 18-19 22.679940460682495 26.094540818553202 29.005182909491513 22.220335811272797 20-21 23.213622312520183 25.518986847863665 29.059758404370367 22.207632435245788 22-23 22.85200179937022 25.487837801422202 29.03747745620896 22.62268294299862 24-25 22.62537587752986 25.731858457564798 29.174958640036447 22.467807024868893 26-27 22.168876737207377 26.173524887661152 28.92293101335864 22.734667361772832 28-29 22.3018385530711 26.388884296345577 28.980142357085747 22.329134793497577 30-31 22.349354464848133 25.900500457140403 28.89788657296804 22.852258505043423 32-33 22.136110510434253 26.02892639316802 28.869207628970834 22.96575546742689 34-35 22.868579932809247 26.207088440932164 28.560583353681896 22.363748272576693 36-37 23.119658762576236 25.70777156296388 28.47193554962638 22.7006341248335 38-39 22.484420590095926 25.99541019236164 28.535293299882586 22.98487591765985 40-41 22.766124050523686 25.817755097554034 28.712497638241985 22.703623213680295 42-43 22.674663197043966 25.86324689968042 28.400813494500415 23.0612764087752 44-45 22.410904516881903 26.19340261191378 28.531281973299038 22.86441089790528 46-47 22.80988481403985 26.536294933715425 27.792675189874505 22.861145062370216 48-49 22.815546097152446 26.349818427422296 27.801985801816205 23.032649673609054 50-51 22.96918614722049 26.641268890484444 27.53387692441955 22.855668037875514 52-53 23.089991805313556 26.505215807183223 27.51499025992593 22.889802127577287 54-55 22.60820468184904 26.613324570988627 27.64130231229704 23.137168434865295 56-57 22.623645598977635 26.847271099698595 27.57394869258857 22.955134608735207 58-59 22.738128964160534 26.28688629993392 27.58763176954738 23.387352966358165 60-61 22.435867557742586 26.515260451944343 27.97054781863914 23.078324171673927 62-63 22.512220889688702 26.557361821636494 27.940285896756123 22.990131391918673 64-65 22.256188922954763 26.507100858621996 27.784133104508513 23.452577113914728 66-67 22.20248987838192 26.6939992225328 27.756438565546564 23.34707233353872 68-69 22.531712813403022 26.219203898345118 27.972960040541096 23.27612324771076 70-71 22.40242003374308 26.40666279548543 27.600794931033633 23.590122239737855 72-73 22.461945085878728 26.5305687286047 27.456614339954477 23.550871845562092 74-75 22.329299040688824 26.872696474657875 27.324285789374684 23.473718695278617 76-77 22.462000964624593 26.539853904663484 27.410115202886864 23.588029927825055 78-79 22.58588735543158 26.56659002410348 27.373593075095666 23.473929545369273 80-81 22.576459164066858 26.817049234519946 27.16743868928328 23.439052912129917 82-83 22.613323318700377 26.70035806059492 27.154322430408605 23.5319961902961 84-85 22.590734275472016 26.499249538492204 27.12403085015777 23.78598533587801 86-87 22.788582380190224 26.430061740270403 27.01082289288767 23.7705329866517 88-89 22.67661599761433 26.452230714451492 26.94802768911961 23.923125598814572 90-91 22.51281670626313 26.499630265859757 27.023490558099343 23.964062469777765 92-93 22.68766451922163 25.951263053063517 27.20711756125177 24.153954866463078 94-95 22.45866702285195 25.902428815566005 27.310479286315775 24.328424875266272 96-97 22.699020405343013 25.702966323241277 27.194070692612648 24.403942578803058 98-99 22.604111551508943 25.81545639358971 26.84034418801312 24.740087866888228 100-101 22.92756780080099 25.16405988001563 27.187755975187812 24.720616343995577 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1371.0 1 939.0 2 718.5 3 970.0 4 822.5 5 805.0 6 1508.0 7 2984.0 8 4627.0 9 5144.5 10 4264.0 11 3461.5 12 3450.5 13 3634.0 14 3885.5 15 4074.0 16 4224.0 17 4474.0 18 4791.5 19 5223.5 20 5850.5 21 6842.5 22 8173.0 23 9774.0 24 11439.5 25 13590.5 26 16442.5 27 19571.0 28 22578.5 29 25926.5 30 30309.0 31 36885.5 32 45491.0 33 48898.0 34 48937.0 35 52007.0 36 56889.5 37 61842.0 38 66108.0 39 69374.0 40 73045.0 41 80691.0 42 87965.0 43 92722.5 44 97869.5 45 104753.0 46 108805.5 47 110083.5 48 111369.5 49 110236.5 50 109210.0 51 108159.0 52 108150.0 53 110646.0 54 112820.5 55 108290.0 56 98694.5 57 90980.5 58 85059.0 59 76012.0 60 63223.0 61 53095.0 62 45260.0 63 36342.0 64 28575.5 65 22719.5 66 17433.0 67 13955.0 68 10794.5 69 7607.0 70 5721.5 71 4270.0 72 3390.5 73 2750.5 74 2097.0 75 1652.5 76 1146.0 77 745.0 78 508.5 79 332.5 80 189.0 81 100.5 82 63.5 83 37.5 84 24.0 85 16.0 86 14.0 87 11.5 88 8.5 89 8.5 90 6.5 91 3.0 92 2.0 93 0.5 94 0.5 95 2.5 96 2.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.300930027157745 2 0.03388107181861694 3 0.04128532556239274 4 0.04383053778681567 5 0.05781267766903515 6 0.056556598908930326 7 0.02928646793297035 8 0.03252582894587226 9 0.03910371508431594 10-11 0.031269750185767435 12-13 0.03272415717115197 14-15 0.026906529229613844 16-17 0.020874045710689362 18-19 0.024196043484124487 20-21 0.03644281139514651 22-23 0.02963354232720984 24-25 0.05059022479843242 26-27 0.04262404108303078 28-29 0.03510409587450848 30-31 0.03480660353658892 32-33 0.030476437284648602 34-35 0.03019547229883568 36-37 0.029203831172437137 38-39 0.017932177035707014 40-41 0.017469411176721027 42-43 0.018494107007332856 44-45 0.0100155753766253 46-47 0.0174198291204011 48-49 0.015353910107070799 50-51 0.00727203492692266 52-53 0.005189588561485717 54-55 0.007354671687455872 56-57 0.004958205631992722 58-59 0.007106761405856235 60-61 0.0059829014626045516 62-63 0.004627658589859874 64-65 0.008809078672840405 66-67 0.002644376337062785 68-69 0.006924960532683169 70-71 0.012279822615235312 72-73 0.012659951713688083 74-75 0.01008168478505187 76-77 0.009156153067079894 78-79 0.0068257964200433155 80-81 0.007453835800095726 82-83 0.012593842305261515 84-85 0.00494167827988608 86-87 0.0011238599432516838 88-89 0.01857674376786607 90-91 0.02702222069436034 92-93 0.012412041432088449 94-95 0.021320284217568708 96-97 0.01707275472616161 98-99 0.012279822615235312 100-101 0.1039405173986741 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3025288.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.16134550368467 #Duplication Level Percentage of deduplicated Percentage of total 1 72.2485135495061 32.62840082536888 2 13.708642494694656 12.382014801787982 3 5.350058349016395 7.248475006944065 4 2.7311323113119683 4.933664397097467 5 1.5326122503662287 3.4607415680984466 6 0.9242632896586226 2.504458425638714 7 0.6459890747802044 2.0421615058428086 8 0.4549511494206296 1.6436964837026815 9 0.35544422994324787 1.4447105708182342 >10 1.8207246016389769 14.908245666170956 >50 0.1336770297867365 4.160419421490897 >100 0.08481757585395333 7.173593079035029 >500 0.00532689330342065 1.6747992576847333 >1k 0.0036252468313336663 2.7580644293327286 >5k 7.398462921089116E-5 0.24097061837457467 >10k+ 1.479692584217823E-4 0.7955839426118964 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13597 0.44944481318803364 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10214 0.3376207488344911 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7212 0.2383905267862101 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3498 0.1156253553380703 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3435 0.11354290897263335 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3128 0.10339511477915492 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.305470421328482E-5 2 3.305470421328482E-5 0.0 0.0 0.0 3.305470421328482E-5 3 3.305470421328482E-5 0.0 0.0 0.0 6.610940842656964E-5 4 3.305470421328482E-5 0.0 0.0 0.0 6.610940842656964E-5 5 3.305470421328482E-5 0.0 0.0 0.0 6.610940842656964E-5 6 3.305470421328482E-5 0.0 0.0 0.0 1.652735210664241E-4 7 3.305470421328482E-5 0.0 0.0 0.0 1.652735210664241E-4 8 6.610940842656964E-5 0.0 0.0 0.0 1.652735210664241E-4 9 6.610940842656964E-5 0.0 0.0 0.0 1.652735210664241E-4 10-11 6.610940842656964E-5 0.0 0.0 0.0 1.652735210664241E-4 12-13 6.610940842656964E-5 0.0 0.0 0.0 3.966564505594178E-4 14-15 1.487461689597817E-4 0.0 0.0 1.652735210664241E-5 4.4623850687934503E-4 16-17 1.652735210664241E-4 0.0 0.0 3.305470421328482E-5 4.958205631992723E-4 18-19 1.983282252797089E-4 0.0 0.0 3.305470421328482E-5 4.958205631992723E-4 20-21 2.3138292949299372E-4 0.0 0.0 8.263676053321204E-5 5.949846758391267E-4 22-23 2.6443763370627855E-4 0.0 0.0 2.974923379195633E-4 5.949846758391267E-4 24-25 3.305470421328482E-4 0.0 0.0 7.437308447989084E-4 5.949846758391267E-4 26-27 3.305470421328482E-4 0.0 0.0 0.0018014813796240225 6.115120279457692E-4 28-29 3.966564505594178E-4 0.0 0.0 0.005404444138872068 6.280393800524116E-4 30-31 4.297111547727026E-4 0.0 0.0 0.01743635647250774 6.280393800524116E-4 32-33 4.297111547727026E-4 0.0 0.0 0.040095356210714486 6.610940842656964E-4 34-35 5.288752674125571E-4 0.0 0.0 0.0761084564510883 7.272034926922659E-4 36-37 6.445667321590539E-4 0.0 0.0 0.13567303344342754 7.272034926922659E-4 38-39 7.272034926922659E-4 0.0 0.0 0.22657347002996078 7.602581969055508E-4 40-41 7.272034926922659E-4 0.0 0.0 0.3277208649226123 7.602581969055508E-4 42-43 7.602581969055508E-4 0.0 0.0 0.42800883750571844 7.602581969055508E-4 44-45 7.933129011188356E-4 0.0 0.0 0.5544430811215328 7.767855490121932E-4 46-47 8.594223095454052E-4 0.0 0.0 0.7078169086711744 8.09840253225478E-4 48-49 8.924770137586901E-4 0.0 0.0 0.8682809702745655 8.924770137586901E-4 50-51 8.924770137586901E-4 0.0 0.0 1.0470077559557964 8.924770137586901E-4 52-53 8.924770137586901E-4 0.0 0.0 1.2179171040905858 0.0010246958306118293 54-55 8.924770137586901E-4 3.305470421328482E-5 0.0 1.4081634541901464 0.0010246958306118293 56-57 9.585864221852597E-4 3.305470421328482E-5 0.0 1.6279441825042773 0.0010412231827184718 58-59 9.585864221852597E-4 3.305470421328482E-5 0.0 1.867822170980085 0.0010577505348251142 60-61 9.585864221852597E-4 3.305470421328482E-5 0.0 2.1260620476463727 0.0010577505348251142 62-63 9.751137742919022E-4 3.305470421328482E-5 0.0 2.3814426923982115 0.0011899693516782535 64-65 9.916411263985445E-4 3.305470421328482E-5 0.0 2.6457315799355303 0.0012395514079981806 66-67 9.916411263985445E-4 3.305470421328482E-5 0.0 2.947851576444953 0.0012726061122114655 68-69 0.0010246958306118293 3.305470421328482E-5 0.0 3.287703517813841 0.0012891334643181079 70-71 0.001057750534825114 3.305470421328482E-5 0.0 3.642677986360307 0.0013056608164247504 72-73 0.0011238599432516838 3.305470421328482E-5 0.0 4.007618448227078 0.0013717702248513199 74-75 0.0011569146474649686 3.305470421328482E-5 0.0 4.376327146374163 0.0014213522811712472 76-77 0.0011569146474649686 3.305470421328482E-5 0.0 4.778685533410373 0.0014544069853845319 78-79 0.0011569146474649686 3.305470421328482E-5 0.0 5.2144787537583195 0.0014544069853845319 80-81 0.0012230240558915382 3.305470421328482E-5 0.0 5.6786163829691585 0.0014874616895978166 82-83 0.0012230240558915382 3.305470421328482E-5 0.0 6.159033454005041 0.0015866258022376712 84-85 0.0012560787601048231 3.305470421328482E-5 0.0 6.649449573065441 0.0016362078585575985 86-87 0.0012891334643181079 3.305470421328482E-5 0.0 7.150327506009345 0.0017188446190908105 88-89 0.0012891334643181079 4.9582056319927225E-5 0.0 7.674823025113643 0.0018014813796240225 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6760 0.0 49.204906 1 GTATCAA 11265 0.0 44.96781 1 TATCAAC 14660 0.0 34.394333 2 ATCAACG 14790 0.0 34.059883 3 TCAACGC 15075 0.0 33.38444 4 CAACGCA 15240 0.0 33.054176 5 AACGCAG 15720 0.0 32.00778 6 GTACATG 13440 0.0 27.54999 1 ACGCAGA 18110 0.0 27.489166 7 CGCAGAG 18125 0.0 27.413998 8 TACATGG 13590 0.0 26.646547 2 ACATGGG 14065 0.0 24.76679 3 GCAGAGT 19450 0.0 24.617983 9 GTGGTAT 2645 0.0 23.781977 1 GAGTACT 11230 0.0 22.756948 12-13 AGTACTT 11535 0.0 22.052275 12-13 GTACTTT 11925 0.0 21.491827 14-15 GAGTACA 10425 0.0 21.164333 1 TAGGACG 225 7.124618E-8 21.12141 4 CAGAGTA 18630 0.0 20.997417 10-11 >>END_MODULE