FastQCFastQC Report
Thu 26 May 2016
SRR938493_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938493_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3025288
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134820.44564352220350595No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107100.3540158821242804No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77460.2560417388361042No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA36760.12150909268803499No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA35180.116286449422336No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68950.051.4581381
GTATCAA111650.043.663111
TATCAAC150450.032.4659232
ATCAACG152000.032.0266763
TCAACGC153800.031.682764
CAACGCA157350.030.967965
AACGCAG161650.030.1451766
GTGGTAT26900.027.4049431
GTACATG133500.027.289591
TACATGG134750.026.6128922
ACGCAGA186800.025.9339987
CGCAGAG188300.025.6265168
ACATGGG140050.024.9833223
TGGTATC28600.024.6119022
GCAGAGT203600.023.327519
GAGTACT116600.022.2402812-13
TAAGGTG16250.021.9413535
GTGTAAG10050.021.7691421
AGGTAAG13050.021.5026022
CATGGGA70950.021.1733784
Produced by FastQC (version 0.11.5)