##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938493_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3025288 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.808729615163912 31.0 31.0 34.0 30.0 34.0 2 32.04245281771521 33.0 31.0 34.0 30.0 34.0 3 32.10656638310138 33.0 31.0 34.0 30.0 34.0 4 35.783765710900916 37.0 35.0 37.0 35.0 37.0 5 35.65667896742393 37.0 35.0 37.0 33.0 37.0 6 35.6906248264628 37.0 35.0 37.0 33.0 37.0 7 35.62279987888756 37.0 35.0 37.0 33.0 37.0 8 35.608139787021926 37.0 35.0 37.0 33.0 37.0 9 37.17404095081196 39.0 37.0 39.0 33.0 39.0 10-11 37.10928281869363 39.0 37.0 39.0 33.0 39.0 12-13 37.00275791924604 39.0 37.0 39.0 33.0 39.0 14-15 38.052970659322355 40.0 37.0 41.0 33.0 41.0 16-17 37.98612594900056 40.0 37.0 41.0 33.0 41.0 18-19 38.001526135693524 40.0 37.0 41.0 33.0 41.0 20-21 37.99767443628508 40.0 37.0 41.0 33.0 41.0 22-23 38.01568660570498 40.0 37.0 41.0 33.0 41.0 24-25 37.943340600960966 40.0 37.0 41.0 33.0 41.0 26-27 37.77368105119248 40.0 37.0 41.0 32.0 41.0 28-29 37.63648171678201 40.0 37.0 41.0 32.0 41.0 30-31 37.38265100711073 39.0 36.0 41.0 31.5 41.0 32-33 37.152010155727325 39.0 36.0 41.0 31.0 41.0 34-35 36.97036034255251 39.0 35.5 41.0 31.0 41.0 36-37 36.96187106814293 39.0 35.5 41.0 31.0 41.0 38-39 36.81068182599475 39.0 35.0 41.0 30.0 41.0 40-41 36.61957919378254 39.0 35.0 41.0 30.0 41.0 42-43 36.37614253585113 39.0 35.0 40.0 30.0 41.0 44-45 36.0797707524044 39.0 35.0 40.0 29.5 41.0 46-47 35.681699725778174 38.0 35.0 40.0 28.5 41.0 48-49 35.25944224153204 38.0 34.0 40.0 27.0 41.0 50-51 34.88200379600223 38.0 34.0 40.0 26.0 41.0 52-53 34.37575282088846 38.0 33.0 40.0 24.0 41.0 54-55 34.05687722953979 37.0 33.0 40.0 24.0 41.0 56-57 33.7018444194404 37.0 33.0 40.0 23.0 41.0 58-59 33.25610933570622 36.5 32.0 39.5 22.0 41.0 60-61 32.86334970422651 36.0 31.0 39.0 21.0 40.5 62-63 32.402278559925534 35.5 31.0 39.0 19.5 40.0 64-65 31.919433455591665 35.0 31.0 38.5 18.0 40.0 66-67 31.449424153997903 35.0 30.0 38.0 15.5 40.0 68-69 30.90733312002031 34.5 29.5 37.5 12.0 39.5 70-71 30.356836770581843 34.0 29.0 37.0 8.0 39.0 72-73 29.8012081824937 34.0 28.5 36.0 7.0 39.0 74-75 29.216654249116118 34.0 28.0 36.0 4.0 38.5 76-77 27.938607332591147 31.5 26.0 34.5 2.0 36.5 78-79 28.58196227929374 33.0 27.0 35.0 2.0 37.0 80-81 28.321543271252192 33.0 27.0 35.0 2.0 36.5 82-83 27.864505792506367 33.0 26.0 35.0 2.0 36.0 84-85 27.313387022987563 33.0 26.0 35.0 2.0 36.0 86-87 26.69859167127229 32.0 25.0 35.0 2.0 35.0 88-89 25.990534289627963 32.0 23.5 34.0 2.0 35.0 90-91 25.485660208218192 31.5 21.5 34.0 2.0 35.0 92-93 24.751767269760762 31.0 18.5 34.0 2.0 35.0 94-95 23.998520801986455 31.0 11.5 34.0 2.0 35.0 96-97 23.05485461218899 30.5 2.0 34.0 2.0 35.0 98-99 21.91658248735327 30.0 2.0 34.0 2.0 35.0 100-101 18.77120624548803 24.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.22118741950116672 1101 2 0.2695338875622184 1101 3 0.2681652113977293 1101 4 0.19149446830013517 1101 5 0.19256510212414923 1101 6 0.20989587013875877 1101 7 0.2338678293867389 1101 8 0.145104676890206 1101 9 0.2750688312351528 1101 10-11 0.2989765920248999 1101 12-13 0.22437167772284994 1101 14-15 0.39383226673115246 1101 16-17 0.4309298122777321 1101 18-19 0.5904451783594027 1101 20-21 0.5077522144050377 1101 22-23 0.4990641699417395 1101 24-25 0.5027142255127899 1101 26-27 0.4066216082809646 1101 28-29 0.3374767599263677 1101 30-31 0.40928285129270847 1101 32-33 0.3372235622077113 1101 34-35 0.18143909758815369 1101 36-37 0.3544535814719296 1101 38-39 0.519884413642103 1101 40-41 0.6925733095231124 1101 42-43 0.48101728608762784 1101 44-45 0.4891447086139209 1101 46-47 0.662182238487361 1101 48-49 0.7127676000782586 1101 50-51 0.8005760300193501 1101 52-53 0.7680793729224931 1101 54-55 0.9368291749694961 1101 56-57 0.8522845043451994 1101 58-59 0.971061627263829 1101 60-61 0.9918228044460164 1101 62-63 1.0823467424876867 1101 64-65 1.0514811598396427 1101 66-67 1.0891595091831512 1101 68-69 1.0549045412618625 1101 70-71 1.1262297011862827 1101 72-73 1.3467080702912035 1101 74-75 1.2349095653971176 1101 76-77 1.090419483903279 1101 78-79 1.3791828944795057 1101 80-81 1.612546556096202 1101 82-83 1.6736180899481603 1101 84-85 2.0537332694130974 1101 86-87 1.9305229832916417 1101 88-89 1.9581443412320283 1101 90-91 2.138132469167509 1101 92-93 2.3861233123773857 1101 94-95 2.492960571893665 1101 96-97 2.9351665248221224 1101 98-99 3.342212239147507 1101 100-101 3.842781690201189 1102 1 0.2956151020844011 1102 2 0.29061591287090494 1102 3 0.24046325002296953 1102 4 0.1646048554029349 1102 5 0.137665289910899 1102 6 0.1164316019006506 1102 7 0.17296288405680116 1102 8 0.12652103789803704 1102 9 0.2491693159675492 1102 10-11 0.2725696203546306 1102 12-13 0.24098648346044627 1102 14-15 0.4039878076950103 1102 16-17 0.35051539884296545 1102 18-19 0.40595779994330883 1102 20-21 0.4061708743018997 1102 22-23 0.4077256421508295 1102 24-25 0.30990281945425835 1102 26-27 0.3522721910890638 1102 28-29 0.3906162281845553 1102 30-31 0.34006147622526584 1102 32-33 0.2670597681067548 1102 34-35 0.4359754650646579 1102 36-37 0.5089552355846365 1102 38-39 0.6055247218521558 1102 40-41 0.5920289091790423 1102 42-43 0.46924583106792994 1102 44-45 0.42661959718959963 1102 46-47 0.39886889670571435 1102 48-49 0.5419110025176792 1102 50-51 0.5283435146109383 1102 52-53 0.5367036502502032 1102 54-55 0.47968177700796844 1102 56-57 0.7046593027218933 1102 58-59 0.6940610431313203 1102 60-61 0.6012229463665193 1102 62-63 0.898759471836378 1102 64-65 0.8200485574386995 1102 66-67 0.8151809394573881 1102 68-69 0.9449161542064637 1102 70-71 1.1232210702451049 1102 72-73 1.2011254503941728 1102 74-75 1.3223552473474243 1102 76-77 1.1993169791040508 1102 78-79 1.5502064368318997 1102 80-81 1.6947145520477065 1102 82-83 1.6701377615513735 1102 84-85 1.7452052833319023 1102 86-87 1.9601160562329234 1102 88-89 2.0984249478933705 1102 90-91 2.185904540384275 1102 92-93 2.311405097062522 1102 94-95 2.460383822598036 1102 96-97 3.0076445431769905 1102 98-99 3.3063077469036415 1102 100-101 3.760004188922089 1103 1 0.21492232194607297 1103 2 0.2857905517990673 1103 3 0.23809473950420568 1103 4 0.14239777218610072 1103 5 0.055482674127041776 1103 6 0.1122582244104251 1103 7 0.2205620171956042 1103 8 0.2436139813275986 1103 9 0.2500880260717864 1103 10-11 0.38608216713350174 1103 12-13 0.3795060199968674 1103 14-15 0.47442940285613133 1103 16-17 0.5265306201065343 1103 18-19 0.4657278402016871 1103 20-21 0.48542428943837024 1103 22-23 0.45961156405719095 1103 24-25 0.3911787357031784 1103 26-27 0.33790085578940676 1103 28-29 0.32348462778603704 1103 30-31 0.45190429134519405 1103 32-33 0.44102446359616465 1103 34-35 0.5178847911732802 1103 36-37 0.5048549059831089 1103 38-39 0.4557786502271668 1103 40-41 0.39745033577214883 1103 42-43 0.5966849939246828 1103 44-45 0.7503075245328716 1103 46-47 0.7719304367626023 1103 48-49 0.6884551745037726 1103 50-51 0.7182268086309875 1103 52-53 0.8283521041683386 1103 54-55 0.8125092225370807 1103 56-57 0.8424713970370235 1103 58-59 1.0865051480420007 1103 60-61 1.0546407737267245 1103 62-63 1.0395418521343132 1103 64-65 0.9018400749577218 1103 66-67 1.0331093722159217 1103 68-69 1.1777141311802986 1103 70-71 1.104934223721802 1103 72-73 1.3148432925871063 1103 74-75 1.3275855549361353 1103 76-77 1.1729173539913305 1103 78-79 1.4823055693143878 1103 80-81 1.664109849417585 1103 82-83 1.8982753751103196 1103 84-85 1.8951485776932309 1103 86-87 2.22990645539074 1103 88-89 2.4656958840220824 1103 90-91 2.6161711750227568 1103 92-93 2.722855107497935 1103 94-95 2.799050636279322 1103 96-97 3.102582055400859 1103 98-99 3.4689058496274825 1103 100-101 3.8828328026923735 1104 1 0.37050729292662865 1104 2 0.34188350454205363 1104 3 0.3175333574317776 1104 4 0.23928199986148968 1104 5 0.2032216461710732 1104 6 0.23884637451960344 1104 7 0.24519177881752796 1104 8 0.29780624507082365 1104 9 0.37432859899375615 1104 10-11 0.27761844427205773 1104 12-13 0.36352269628011413 1104 14-15 0.5093489064142673 1104 16-17 0.5363084205115598 1104 18-19 0.5671007485952657 1104 20-21 0.6122924517662582 1104 22-23 0.5332378071417381 1104 24-25 0.5612281762444269 1104 26-27 0.5329816916400247 1104 28-29 0.6684681664616647 1104 30-31 0.7618778174626968 1104 32-33 0.6779798539727864 1104 34-35 0.6613896390615892 1104 36-37 0.7044272509581617 1104 38-39 0.8191835988067382 1104 40-41 0.8538589386525999 1104 42-43 0.8346048708324432 1104 44-45 0.8790028372980387 1104 46-47 0.8388307297854922 1104 48-49 1.0459063279278453 1104 50-51 0.9083239926697289 1104 52-53 1.1016779705091793 1104 54-55 1.050335762038607 1104 56-57 1.2282858083325436 1104 58-59 1.3148084437009615 1104 60-61 1.177687759211338 1104 62-63 1.3574349157508223 1104 64-65 1.2314568824790264 1104 66-67 1.2704013429362568 1104 68-69 1.4413244220535937 1104 70-71 1.5488186437288185 1104 72-73 1.6782369275416826 1104 74-75 1.6492375704584248 1104 76-77 1.5292654289780394 1104 78-79 1.7949547677440556 1104 80-81 2.0296672151531396 1104 82-83 2.1579626665153597 1104 84-85 2.3428117484497726 1104 86-87 2.423145998772835 1104 88-89 2.560116569854028 1104 90-91 2.713134598073136 1104 92-93 2.981838125746812 1104 94-95 3.250260628472919 1104 96-97 3.564803033124331 1104 98-99 4.044989977742972 1104 100-101 4.327531803699284 1105 1 0.3178270518259829 1105 2 0.2682836637174617 1105 3 0.2950220036616571 1105 4 0.19235169197395408 1105 5 0.11847604853978311 1105 6 0.16304465364424914 1105 7 0.2208034353548456 1105 8 0.23827547858002873 1105 9 0.3197553671764837 1105 10-11 0.30931682079424405 1105 12-13 0.40587149355965835 1105 14-15 0.4885840992057311 1105 16-17 0.4490123987835588 1105 18-19 0.5006791063058103 1105 20-21 0.46743205026578494 1105 22-23 0.4628903483826363 1105 24-25 0.3845114750712071 1105 26-27 0.5314876829799928 1105 28-29 0.47038661967745554 1105 30-31 0.4871885851779254 1105 32-33 0.45481391917905256 1105 34-35 0.5690772962890449 1105 36-37 0.6551635763438526 1105 38-39 0.6151809774215806 1105 40-41 0.5185870464688946 1105 42-43 0.6314836971642919 1105 44-45 0.48413478313388225 1105 46-47 0.6237599217755161 1105 48-49 0.6743162852955535 1105 50-51 0.740452727169604 1105 52-53 0.8607369850687192 1105 54-55 0.923939150916361 1105 56-57 0.8956151261045378 1105 58-59 1.05334823198654 1105 60-61 1.0206453606717503 1105 62-63 1.104423940439709 1105 64-65 1.1485274010791677 1105 66-67 1.3244044513091104 1105 68-69 1.243520458252089 1105 70-71 1.2673644567478064 1105 72-73 1.2906625575646125 1105 74-75 1.4166425104615428 1105 76-77 1.3606490723138194 1105 78-79 1.6517946489747146 1105 80-81 1.7785561875256342 1105 82-83 1.8351454844765804 1105 84-85 1.8433710885953722 1105 86-87 2.092518925596675 1105 88-89 2.205747803296447 1105 90-91 2.502910013264927 1105 92-93 2.736908015437141 1105 94-95 2.7911362879933925 1105 96-97 3.162184917738859 1105 98-99 3.557431469650627 1105 100-101 4.188734398926247 1106 1 0.12804616490961962 1106 2 0.21617056203599816 1106 3 0.16896387419640746 1106 4 0.18968426756752166 1106 5 0.1405704773483265 1106 6 0.14476598582292866 1106 7 0.14583049748951282 1106 8 0.12840591806909885 1106 9 0.10564156187952278 1106 10-11 0.20570921272611997 1106 12-13 0.12056736026963222 1106 14-15 0.18094468410867393 1106 16-17 0.15643824853458455 1106 18-19 0.37328726463734085 1106 20-21 0.4499943382790619 1106 22-23 0.2858706110657607 1106 24-25 0.1529958151159434 1106 26-27 0.32282171090812284 1106 28-29 0.29924928836476283 1106 30-31 0.33415469436461365 1106 32-33 0.3142015481752267 1106 34-35 0.27336190875927713 1106 36-37 0.2880061829686653 1106 38-39 0.3139040957011545 1106 40-41 0.270039155511256 1106 42-43 0.42337588161979767 1106 44-45 0.013883060177974471 1106 46-47 -0.21243049642466616 1106 48-49 0.24349770082561406 1106 50-51 0.001991344835765574 1106 52-53 0.17649594263528456 1106 54-55 0.04757454277606854 1106 56-57 0.19779926588947916 1106 58-59 0.5263956989707381 1106 60-61 0.48654436478519614 1106 62-63 0.566289784417485 1106 64-65 0.10328424779924106 1106 66-67 0.3870959159118357 1106 68-69 0.2797020812585096 1106 70-71 0.4649999027317868 1106 72-73 0.8547833539130281 1106 74-75 1.0634320392195704 1106 76-77 1.1968020545610578 1106 78-79 1.270907056061386 1106 80-81 1.212767687128725 1106 82-83 1.5004049201408627 1106 84-85 1.5204296149719276 1106 86-87 1.8123708055129626 1106 88-89 2.1502115670981325 1106 90-91 2.1778286013431547 1106 92-93 1.9608099693232646 1106 94-95 2.4945041706519753 1106 96-97 2.99332779678916 1106 98-99 3.499309897521016 1106 100-101 3.8888379115913274 1107 1 0.11634548695506552 1107 2 0.06267773336431048 1107 3 0.06096682108428553 1107 4 0.06647837719856398 1107 5 0.0594727190272053 1107 6 -0.0027862234912774397 1107 7 0.037284522631743755 1107 8 0.10309249184349767 1107 9 0.15490696916327096 1107 10-11 0.16828586587912042 1107 12-13 0.2378581823321042 1107 14-15 0.37003076559948767 1107 16-17 0.34373873741136407 1107 18-19 0.3350324440057193 1107 20-21 0.21856377751866063 1107 22-23 0.1345819863936626 1107 24-25 0.203092270333471 1107 26-27 0.2020834132894791 1107 28-29 0.20530829425853625 1107 30-31 0.19077241657358712 1107 32-33 0.1908246786899923 1107 34-35 0.20099302823599174 1107 36-37 0.30326718695462773 1107 38-39 0.3161883596605364 1107 40-41 0.43495777541382097 1107 42-43 0.4790101012906973 1107 44-45 0.46703265306455677 1107 46-47 0.5000963321788774 1107 48-49 0.5352195861524933 1107 50-51 0.5434170508964726 1107 52-53 0.629396412774625 1107 54-55 0.6151106549281522 1107 56-57 0.5469109410163853 1107 58-59 0.6705702722681863 1107 60-61 0.6336458109746843 1107 62-63 0.6841667901749915 1107 64-65 0.7870799841805258 1107 66-67 0.9965506564834961 1107 68-69 1.0497179229101299 1107 70-71 0.9329608011067414 1107 72-73 1.0892091366268914 1107 74-75 1.1011421966140453 1107 76-77 1.1285358211266576 1107 78-79 1.2366673701689699 1107 80-81 1.3847782141617344 1107 82-83 1.4841991660441813 1107 84-85 1.8414402442091458 1107 86-87 2.000665656124486 1107 88-89 2.119005642661108 1107 90-91 2.1487936645394186 1107 92-93 2.409980411867469 1107 94-95 2.387930949856827 1107 96-97 2.756872195998781 1107 98-99 3.0408069335991 1107 100-101 3.3994173242578363 1108 1 0.19120571612132764 1108 2 0.17469464740444351 1108 3 0.16435180046446618 1108 4 0.11671746735316191 1108 5 0.08738064398522027 1108 6 0.03593142334047883 1108 7 0.12768842656463164 1108 8 0.07841867740254571 1108 9 0.1788614222073477 1108 10-11 0.16648404451557752 1108 12-13 0.24022375141975516 1108 14-15 0.24129463009415986 1108 16-17 0.3545277510056408 1108 18-19 0.3423312077817542 1108 20-21 0.28368769747131495 1108 22-23 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-0.0862967051089285 2316 56-57 0.009932153356764672 2316 58-59 0.09111445012947428 2316 60-61 0.2921990052983716 2316 62-63 0.35240878849819524 2316 64-65 0.43492691354997604 2316 66-67 0.5134071680122041 2316 68-69 0.4788892083888783 2316 70-71 0.5074800602615817 2316 72-73 0.7231667414671605 2316 74-75 0.9328815631368954 2316 76-77 0.8570663248440944 2316 78-79 0.9171445002224203 2316 80-81 0.944554621835433 2316 82-83 1.122187776090481 2316 84-85 1.281814762831754 2316 86-87 1.3775532957405332 2316 88-89 1.5053940280523292 2316 90-91 1.5172799402098072 2316 92-93 1.5055894529799403 2316 94-95 2.0527168651491685 2316 96-97 2.291370432485895 2316 98-99 2.522417081012396 2316 100-101 3.1143901538343677 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10.0 3 20.0 4 42.0 5 111.0 6 248.0 7 639.0 8 1381.0 9 2453.0 10 4074.0 11 6343.0 12 9205.0 13 12634.0 14 16261.0 15 21521.0 16 27117.0 17 35043.0 18 33026.0 19 27431.0 20 26945.0 21 28185.0 22 30777.0 23 35003.0 24 40225.0 25 47380.0 26 56428.0 27 67162.0 28 80708.0 29 95739.0 30 114321.0 31 136078.0 32 162554.0 33 200075.0 34 251043.0 35 322938.0 36 408575.0 37 457434.0 38 251155.0 39 15004.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.283988120127525 18.858785205360817 14.540448255288343 23.316778419223315 2 16.366166461306673 20.60329541614004 38.43899286960996 24.591545252943327 3 19.255508158735456 24.074128553177633 29.8953982847897 26.774965003297208 4 13.439117201403636 16.641291672065602 35.417619743971485 34.50197138255928 5 14.020037984819309 37.303011653530795 33.52918014995401 15.147770211695882 6 33.34763500202295 35.368930164665315 16.616996464468837 14.666438368842899 7 29.40691927512356 32.832047725704136 19.714056975732557 18.04697602343975 8 27.445122580065107 34.40842656963569 19.12750124946782 19.01894960083139 9 27.184387073230713 16.08260106145266 19.385063504697733 37.34794836061889 10-11 25.643608145736867 26.267499160410512 26.62896887833489 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23.126731921164385 25.718411559506688 28.626865632172095 22.527990887156836 38-39 22.445598129654705 26.003902282921466 28.76817913739844 22.782320450025388 40-41 22.75958390873562 25.81881501426006 28.819452192231054 22.602148884773264 42-43 22.705630363757443 25.924971567116728 28.42747850364938 22.941919565476454 44-45 22.518128511536567 26.16660611984568 28.617628479637926 22.697636888979826 46-47 22.829522314347624 26.56774924804593 27.880964583661473 22.721763853944978 48-49 22.84374248005479 26.337509023934253 27.894749194126312 22.92399930188465 50-51 22.963698001644804 26.647991860609633 27.640409772557188 22.74790036518837 52-53 23.26388429795775 26.453679781891843 27.490969454808926 22.791466465341482 54-55 22.662916720656018 26.622390992196443 27.644277172950144 23.07041511419739 56-57 22.723191973788943 26.806786659650257 27.546468303183037 22.92355306337777 58-59 22.766559745716773 26.295612186343913 27.614643630622936 23.323184437316378 60-61 22.44439207110199 26.589914745306892 27.92750640600168 23.038186777589438 62-63 22.501857674376787 26.726662056637252 27.80338599168079 22.968094277305166 64-65 22.20420006293616 26.600674051528316 27.70430451580147 23.490821369734054 66-67 22.20703355802309 26.715571190459077 27.710635287778913 23.36675996373892 68-69 22.533548519848974 26.369322534341833 27.826492236675413 23.270636709133775 70-71 22.39723953554174 26.609466602849054 27.42176943153842 23.571524430070788 72-73 22.47279349033662 26.735083316062248 27.22801175557889 23.564111438022245 74-75 22.33762537649308 27.08512379647822 26.979646235333625 23.59760459169507 76-77 22.421534743138505 26.798506456244827 27.295087277641006 23.484871522975663 78-79 22.46690232467124 26.859459330814122 27.288013570939363 23.385624773575277 80-81 22.48325159179086 27.17864004660714 27.067014292030095 23.2710940695719 82-83 22.530071436965056 27.0255279349225 27.035097377757804 23.40930325035464 84-85 22.46420874632311 26.927977513808838 26.922655455745847 23.685158284122203 86-87 22.602024398358548 26.90737889119375 26.85948193608814 23.63111477435956 88-89 22.42068273191932 27.081248820359853 26.609739836253553 23.888328611467273 90-91 22.30310592882436 27.065816602691502 26.89383152701625 23.73724594146789 92-93 22.353769296357576 26.642596163428866 27.00051350627273 24.00312103394083 94-95 22.136818048397373 26.590823749672758 27.054961378883597 24.21739682304627 96-97 22.319333286505827 26.457121843791704 26.949372580291957 24.27417228941051 98-99 22.120191532178094 26.72786855334104 26.62842347571537 24.5235164387655 100-101 22.09752046310031 26.405064023369874 26.937331884423255 24.560083629106565 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1346.0 1 914.0 2 711.0 3 947.5 4 779.5 5 785.0 6 1495.0 7 3137.0 8 4826.5 9 5236.0 10 4282.5 11 3355.0 12 3256.5 13 3396.5 14 3650.5 15 3942.0 16 4129.0 17 4372.0 18 4756.5 19 5158.5 20 5712.0 21 6629.0 22 7822.0 23 9216.0 24 10931.5 25 13140.0 26 15861.0 27 19134.5 28 22140.5 29 25648.5 30 30366.5 31 37228.5 32 46877.5 33 49987.5 34 49117.0 35 52579.5 36 57255.5 37 62064.5 38 67091.0 39 70365.0 40 73631.0 41 81692.5 42 89727.0 43 94763.0 44 99756.0 45 106970.5 46 110893.5 47 112772.0 48 114514.0 49 112262.5 50 111192.5 51 110332.0 52 109550.5 53 112083.0 54 115561.0 55 109834.5 56 98272.0 57 90367.0 58 84472.0 59 74076.0 60 60122.0 61 50119.0 62 42969.5 63 34247.0 64 25957.0 65 20068.5 66 14869.5 67 11941.0 68 9016.5 69 5972.0 70 4435.0 71 3381.5 72 2722.5 73 2282.0 74 1803.0 75 1427.0 76 971.5 77 545.5 78 326.0 79 196.5 80 106.0 81 53.5 82 29.0 83 12.5 84 6.5 85 6.0 86 4.5 87 1.5 88 1.5 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 9.916411263985445E-5 2 0.04396275660366881 3 9.916411263985445E-5 4 0.0 5 0.10008964435782643 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0013387155206380352 26-27 0.011866638812569249 28-29 0.0 30-31 0.0 32-33 5.288752674125571E-4 34-35 0.01598194948712321 36-37 0.0035368533508214756 38-39 0.012957444051607647 40-41 0.0076191093211621505 42-43 0.005999428814711195 44-45 0.0026774310412760703 46-47 1.1569146474649685E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.818008731730665E-4 68-69 1.818008731730665E-4 70-71 0.0 72-73 3.305470421328482E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.652735210664241E-5 82-83 0.0011073325911450415 84-85 0.004710295350393087 86-87 0.0014378796332778895 88-89 3.305470421328482E-5 90-91 8.428949574387628E-4 92-93 2.8096498581292096E-4 94-95 0.0 96-97 1.652735210664241E-5 98-99 0.0 100-101 8.428949574387628E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3025288.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.13940014093507 #Duplication Level Percentage of deduplicated Percentage of total 1 69.45410648140107 29.267543844508715 2 14.984925289030945 12.629115256729841 3 5.86295894321599 7.4118471875415715 4 2.916775339560116 4.916446526197419 5 1.7357745928036004 3.657225006030978 6 1.106925621695923 2.7987109019338687 7 0.7229567662077802 2.1325475119078265 8 0.5413166379398369 1.8248606727273968 9 0.3677730532540009 1.394796226691139 >10 2.0364214893017327 15.592192832884713 >50 0.16073343029374582 4.644444391833673 >100 0.09815960420257186 7.7161968453346965 >500 0.006687914386567901 1.930200795406511 >1k 0.004248792669019905 3.0249405552624835 >5k 7.868134572259084E-5 0.25682519171650037 >10k+ 1.5736269144518168E-4 0.8021062532927417 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13482 0.44564352220350595 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10710 0.3540158821242804 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7746 0.2560417388361042 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3676 0.12150909268803499 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3518 0.116286449422336 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 3.305470421328482E-5 5 0.0 0.0 0.0 0.0 3.305470421328482E-5 6 0.0 0.0 0.0 0.0 6.610940842656964E-5 7 0.0 0.0 0.0 0.0 6.610940842656964E-5 8 0.0 0.0 0.0 0.0 6.610940842656964E-5 9 0.0 0.0 0.0 0.0 6.610940842656964E-5 10-11 0.0 0.0 0.0 0.0 9.916411263985445E-5 12-13 0.0 0.0 0.0 0.0 3.6360174634613297E-4 14-15 0.0 0.0 0.0 1.652735210664241E-5 4.4623850687934503E-4 16-17 0.0 0.0 0.0 3.305470421328482E-5 5.454026195191996E-4 18-19 6.610940842656964E-5 0.0 0.0 3.305470421328482E-5 6.610940842656964E-4 20-21 1.3221881685313927E-4 0.0 0.0 8.263676053321204E-5 7.602581969055508E-4 22-23 1.3221881685313927E-4 0.0 0.0 2.974923379195633E-4 8.263676053321204E-4 24-25 1.3221881685313927E-4 0.0 0.0 7.437308447989084E-4 8.263676053321204E-4 26-27 1.3221881685313927E-4 0.0 0.0 0.0019337001964771618 8.924770137586901E-4 28-29 1.8180087317306649E-4 0.0 0.0 0.005734991181004916 8.924770137586901E-4 30-31 1.983282252797089E-4 0.0 0.0 0.017965231739920297 9.090043658653325E-4 32-33 1.983282252797089E-4 0.0 0.0 0.04110352468921967 9.255317179719749E-4 34-35 1.983282252797089E-4 0.0 0.0 0.07748022667593962 9.585864221852597E-4 36-37 2.3138292949299372E-4 0.0 0.0 0.13782158921729104 9.585864221852597E-4 38-39 2.6443763370627855E-4 0.0 0.0 0.23019296014131546 0.0010246958306118293 40-41 2.6443763370627855E-4 0.0 0.0 0.33244768762511206 0.0010246958306118293 42-43 2.6443763370627855E-4 0.0 0.0 0.4338925748556831 0.0010246958306118293 44-45 2.6443763370627855E-4 0.0 0.0 0.5621613545553349 0.001090805239038399 46-47 3.140196900262058E-4 0.0 0.0 0.7171052805551075 0.0011569146474649686 48-49 3.305470421328482E-4 0.0 0.0 0.8789906944396699 0.0011569146474649686 50-51 3.305470421328482E-4 0.0 0.0 1.0586925938951928 0.0011569146474649686 52-53 3.305470421328482E-4 0.0 0.0 1.2303126181705677 0.0012560787601048231 54-55 3.966564505594178E-4 0.0 0.0 1.4217158829175933 0.0012891334643181079 56-57 4.958205631992723E-4 0.0 0.0 1.6432319832029214 0.0012891334643181079 58-59 4.958205631992723E-4 0.0 0.0 1.8839528666361682 0.0012891334643181079 60-61 4.958205631992723E-4 0.0 0.0 2.144539627301599 0.0012891334643181079 62-63 4.958205631992723E-4 0.0 0.0 2.401804390193595 0.0012891334643181079 64-65 4.958205631992723E-4 0.0 0.0 2.6682253061526704 0.0012891334643181079 66-67 5.619299716258419E-4 0.0 0.0 2.9733036986891825 0.0013056608164247504 68-69 5.619299716258419E-4 0.0 0.0 3.316808184873638 0.0013221881685313928 70-71 6.445667321590539E-4 0.0 0.0 3.6778647189953486 0.0013387155206380352 72-73 6.610940842656964E-4 0.0 0.0 4.049961524324296 0.0013882975769579625 74-75 6.941487884789812E-4 0.0 0.0 4.429049399594353 0.0014378796332778895 76-77 7.272034926922659E-4 0.0 0.0 4.843142206626278 0.0014544069853845319 78-79 7.602581969055508E-4 0.0 0.0 5.293314223307004 0.0014874616895978166 80-81 9.420590700786173E-4 0.0 0.0 5.774491552539791 0.001537043745917744 82-83 9.916411263985445E-4 0.0 0.0 6.276906529229613 0.0016857899148775258 84-85 0.001008168478505187 0.0 0.0 6.790791488281446 0.0016857899148775258 86-87 0.0010246958306118293 0.0 0.0 7.318807333384457 0.0017188446190908105 88-89 0.0010246958306118293 0.0 0.0 7.87571298996988 0.0017518993233040954 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6895 0.0 51.458138 1 GTATCAA 11165 0.0 43.66311 1 TATCAAC 15045 0.0 32.465923 2 ATCAACG 15200 0.0 32.026676 3 TCAACGC 15380 0.0 31.68276 4 CAACGCA 15735 0.0 30.96796 5 AACGCAG 16165 0.0 30.145176 6 GTGGTAT 2690 0.0 27.404943 1 GTACATG 13350 0.0 27.28959 1 TACATGG 13475 0.0 26.612892 2 ACGCAGA 18680 0.0 25.933998 7 CGCAGAG 18830 0.0 25.626516 8 ACATGGG 14005 0.0 24.983322 3 TGGTATC 2860 0.0 24.611902 2 GCAGAGT 20360 0.0 23.32751 9 GAGTACT 11660 0.0 22.24028 12-13 TAAGGTG 1625 0.0 21.941353 5 GTGTAAG 1005 0.0 21.769142 1 AGGTAAG 1305 0.0 21.502602 2 CATGGGA 7095 0.0 21.173378 4 >>END_MODULE