FastQCFastQC Report
Thu 26 May 2016
SRR938492_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938492_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3027259
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127250.42034725142447343No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96280.3180434842212047No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67200.22198298857150972No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA34570.11419571301959958No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA33770.11155305839374828No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64350.051.438821
GTATCAA108800.046.0737881
TATCAAC144600.034.4813272
ATCAACG144250.034.4331933
TCAACGC147200.033.8722844
CAACGCA149850.033.176475
AACGCAG156050.031.9097636
GTGGTAT23300.028.863121
GTACATG132250.028.0945361
TACATGG134850.027.4576452
ACGCAGA181100.027.3073377
CGCAGAG183850.026.7701238
ACATGGG140750.025.3611153
TAAGGTG13050.024.7658755
TGGTATC27150.024.6845992
GCAGAGT197150.024.263779
GTAAGGT12500.023.5753694
AGGTAAG11150.023.019492
GAGTACT114200.022.75248712-13
CATGGGA74850.021.4635584
Produced by FastQC (version 0.11.5)