##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938492_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3027259 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.99881410873665 31.0 31.0 34.0 28.0 34.0 2 31.165723183909932 31.0 31.0 34.0 27.0 34.0 3 31.211593061578146 31.0 31.0 34.0 27.0 34.0 4 34.89744385927996 37.0 35.0 37.0 32.0 37.0 5 34.88880237865343 37.0 35.0 37.0 32.0 37.0 6 34.86411899345249 37.0 35.0 37.0 32.0 37.0 7 34.77278158228285 37.0 35.0 37.0 32.0 37.0 8 34.72872753867443 37.0 35.0 37.0 31.0 37.0 9 36.18730277125281 39.0 35.0 39.0 31.0 39.0 10-11 36.01102895391507 38.0 35.0 39.0 30.0 39.0 12-13 35.820304440419534 38.0 35.0 39.0 30.0 39.0 14-15 36.952652382898194 39.0 36.0 41.0 31.0 41.0 16-17 36.88431316910776 39.0 36.0 41.0 31.0 41.0 18-19 36.88726237167022 39.0 36.0 41.0 30.5 41.0 20-21 36.82614173415621 39.0 36.0 41.0 30.0 41.0 22-23 36.669726310170354 39.0 36.0 41.0 30.0 41.0 24-25 36.517787708286605 39.0 36.0 41.0 30.0 41.0 26-27 36.28769672499116 39.0 35.0 40.5 29.0 41.0 28-29 36.041490173123606 39.0 35.0 40.0 28.0 41.0 30-31 35.74697672052507 38.0 35.0 40.0 27.0 41.0 32-33 35.4236859152124 38.0 34.0 40.0 26.5 41.0 34-35 35.0964045362488 38.0 34.0 40.0 25.5 41.0 36-37 34.843470281201576 38.0 34.0 40.0 25.0 41.0 38-39 34.57857371965861 38.0 33.0 40.0 24.0 41.0 40-41 34.277398134748296 38.0 33.0 40.0 24.0 41.0 42-43 33.83397109398304 38.0 33.0 40.0 22.5 41.0 44-45 33.331178468707165 37.0 32.0 40.0 20.0 41.0 46-47 32.78238697118417 37.0 31.0 40.0 16.5 41.0 48-49 32.243536479699955 36.5 30.5 39.5 12.5 41.0 50-51 31.492789021355623 35.5 30.0 38.5 9.0 40.0 52-53 31.469195896353767 36.0 30.0 39.0 8.5 40.0 54-55 31.42697073491234 36.0 30.0 39.0 7.0 40.0 56-57 30.981980398769977 35.5 29.0 39.0 2.0 40.0 58-59 30.513097161491633 35.0 28.0 39.0 2.0 40.0 60-61 29.946565358299374 35.0 28.0 38.0 2.0 40.0 62-63 29.210177589694176 34.0 26.5 38.0 2.0 40.0 64-65 28.52721455283476 34.0 26.0 37.5 2.0 40.0 66-67 27.818209971462633 33.5 24.5 37.0 2.0 39.0 68-69 26.943548107380305 32.5 22.5 36.0 2.0 39.0 70-71 26.07943505990072 32.0 19.5 35.5 2.0 38.5 72-73 25.16975042439382 31.0 15.5 35.0 2.0 38.0 74-75 24.21483328648127 30.5 7.5 35.0 2.0 37.0 76-77 23.243699828789012 30.0 3.5 34.0 2.0 36.5 78-79 22.23763311959763 29.0 2.0 34.0 2.0 36.0 80-81 21.24984796477606 28.5 2.0 34.0 2.0 35.0 82-83 20.22511321297583 26.5 2.0 33.0 2.0 35.0 84-85 19.217129753351134 25.5 2.0 32.5 2.0 35.0 86-87 18.192606744252807 24.0 2.0 32.0 2.0 34.5 88-89 17.073344236485877 19.0 2.0 31.5 2.0 34.0 90-91 15.909385685202356 11.5 2.0 31.0 2.0 34.0 92-93 14.734344170749843 2.0 2.0 30.5 2.0 34.0 94-95 13.475329828072194 2.0 2.0 29.5 2.0 34.0 96-97 12.06494340259621 2.0 2.0 29.0 2.0 34.0 98-99 10.423657341509266 2.0 2.0 26.0 2.0 33.0 100-101 7.3485296434827685 2.0 2.0 2.0 2.0 28.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6835022115498184 1101 2 0.5753597954465768 1101 3 0.5872788674745237 1101 4 0.37375978868831794 1101 5 0.46796614053450725 1101 6 0.599957060032601 1101 7 0.6002397336967178 1101 8 0.6052996408631728 1101 9 0.6344853167788287 1101 10-11 0.7603974721485898 1101 12-13 0.7411930181643527 1101 14-15 0.7996917732701974 1101 16-17 0.7514604388481061 1101 18-19 0.792632283307185 1101 20-21 0.8971705383126576 1101 22-23 0.9312224416007311 1101 24-25 0.9658887567495285 1101 26-27 0.9903114126896142 1101 28-29 1.0076157627958082 1101 30-31 1.112004540872178 1101 32-33 1.0662677590165046 1101 34-35 1.0933641210270224 1101 36-37 1.3461806637848213 1101 38-39 1.3784813118662242 1101 40-41 1.3772952232129398 1101 42-43 1.5654431206002357 1101 44-45 1.6297049642500738 1101 46-47 1.692019225204163 1101 48-49 1.368930669939786 1101 50-51 1.4875861329794162 1101 52-53 1.4544005844897328 1101 54-55 1.6081085799675492 1101 56-57 1.937223637458331 1101 58-59 1.9235458778197518 1101 60-61 1.9711594775238588 1101 62-63 1.739699183095361 1101 64-65 1.7230053555722336 1101 66-67 1.8151807260632147 1101 68-69 2.074322332929974 1101 70-71 2.3584430041702014 1101 72-73 2.5633961359972233 1101 74-75 2.610611776856114 1101 76-77 3.0975240213424975 1101 78-79 3.2078080998348497 1101 80-81 3.45367363466179 1101 82-83 3.7465404306794703 1101 84-85 4.065222558208962 1101 86-87 4.026537131635358 1101 88-89 4.374082903968304 1101 90-91 4.381502484395339 1101 92-93 4.351695293961916 1101 94-95 4.248251690785773 1101 96-97 4.459950726070948 1101 98-99 4.03617018763018 1101 100-101 2.815718004085917 1102 1 0.7852995020266356 1102 2 0.6948622533608635 1102 3 0.5535168156169306 1102 4 0.49132790456910413 1102 5 0.5201945790534808 1102 6 0.5265107723939124 1102 7 0.5690590334659689 1102 8 0.629293024728689 1102 9 0.6538969419283802 1102 10-11 0.8269055247299519 1102 12-13 0.8532936488850353 1102 14-15 0.9529461191873594 1102 16-17 1.1127591280585634 1102 18-19 0.9608896823928177 1102 20-21 1.041741809535388 1102 22-23 0.853407301255281 1102 24-25 0.924350419893166 1102 26-27 1.0645272111179054 1102 28-29 0.9942777763784605 1102 30-31 0.7416935215686067 1102 32-33 0.9575809798644954 1102 34-35 1.00185887019245 1102 36-37 1.1021891736059146 1102 38-39 1.3835996819904892 1102 40-41 1.3327688468063457 1102 42-43 1.56253351658669 1102 44-45 1.5326826125962114 1102 46-47 1.4319170891631074 1102 48-49 1.5251626909500757 1102 50-51 1.646569683315505 1102 52-53 1.66741712562834 1102 54-55 1.5971483337454941 1102 56-57 2.002486952174877 1102 58-59 2.0177747073796617 1102 60-61 2.1169667269734767 1102 62-63 2.2045088421809496 1102 64-65 2.3511656122791074 1102 66-67 2.467012199188243 1102 68-69 2.4539701901353013 1102 70-71 2.409247901222386 1102 72-73 2.606591314906474 1102 74-75 2.730163315489861 1102 76-77 2.833414317509199 1102 78-79 3.4051894896563937 1102 80-81 3.678447014135461 1102 82-83 3.7406011955523333 1102 84-85 3.7129852798110825 1102 86-87 3.905135905179062 1102 88-89 3.9757356938528083 1102 90-91 4.027978943436741 1102 92-93 4.020758579738212 1102 94-95 4.106984742223704 1102 96-97 3.939164372704214 1102 98-99 3.676807980212118 1102 100-101 2.4692992650404806 1103 1 0.5383344767249341 1103 2 0.5260800601674127 1103 3 0.4147792740027221 1103 4 0.42417754480754155 1103 5 0.4345234532669693 1103 6 0.4893829389723763 1103 7 0.48349128818311016 1103 8 0.31053004555876385 1103 9 0.4206938154121218 1103 10-11 0.53070129712723 1103 12-13 0.5247615355882758 1103 14-15 0.611076885820431 1103 16-17 0.6580470990447367 1103 18-19 0.8970993183873475 1103 20-21 0.6793350493044059 1103 22-23 0.7863566991511846 1103 24-25 0.745827687180288 1103 26-27 0.8430692225479888 1103 28-29 0.816964362661075 1103 30-31 0.7582127297696957 1103 32-33 0.9866499213335302 1103 34-35 0.8154427319653408 1103 36-37 0.8890049893997158 1103 38-39 1.010030997355365 1103 40-41 1.1010125944854607 1103 42-43 1.1717552774710782 1103 44-45 1.3110053124446992 1103 46-47 1.4395044288683394 1103 48-49 1.2763962053825892 1103 50-51 1.3895581173790674 1103 52-53 1.5665241587372662 1103 54-55 1.6779108911126173 1103 56-57 1.9718506768747943 1103 58-59 1.8878981299859596 1103 60-61 1.9487029889466605 1103 62-63 2.176051708861504 1103 64-65 2.1529134414826103 1103 66-67 2.278559762630099 1103 68-69 2.4055296313371564 1103 70-71 2.3523686985590793 1103 72-73 2.8533822495102186 1103 74-75 2.629412221843168 1103 76-77 2.731198494210936 1103 78-79 2.712706183861002 1103 80-81 2.855124359572546 1103 82-83 2.9379964985322538 1103 84-85 3.1933558581596593 1103 86-87 3.281070243679693 1103 88-89 3.4157806947779257 1103 90-91 3.5962675885310826 1103 92-93 3.5868801158767507 1103 94-95 3.504819250609943 1103 96-97 3.3819907490946424 1103 98-99 3.114942188549062 1103 100-101 2.3000643454611955 1104 1 0.5227411326139375 1104 2 0.5317700429439682 1104 3 0.5179698152801393 1104 4 0.4381465522133823 1104 5 0.5067776107954387 1104 6 0.5718755583534403 1104 7 0.574432043852795 1104 8 0.5765393226784852 1104 9 0.652758491013202 1104 10-11 0.7311058199032558 1104 12-13 0.8246905519301393 1104 14-15 0.8746808858876918 1104 16-17 0.9446394750875697 1104 18-19 0.8436065861050395 1104 20-21 0.9074830123977122 1104 22-23 1.0455121497592827 1104 24-25 1.04705338638734 1104 26-27 1.1037958021445675 1104 28-29 1.1938065147030272 1104 30-31 1.1884421595223031 1104 32-33 1.2110192957885388 1104 34-35 1.1895218060790071 1104 36-37 1.3045916074788053 1104 38-39 1.2738359376258046 1104 40-41 1.335910010488135 1104 42-43 1.525504800042924 1104 44-45 1.384317018382582 1104 46-47 1.418499871981716 1104 48-49 1.5745435439157873 1104 50-51 1.4830434750478219 1104 52-53 1.5371126625074538 1104 54-55 1.9962661120747782 1104 56-57 2.0892456366747396 1104 58-59 2.1569564614976606 1104 60-61 2.2314431503233827 1104 62-63 2.3911454048881 1104 64-65 2.4495686819022175 1104 66-67 2.6361986131512474 1104 68-69 2.9331679774059083 1104 70-71 3.0094759523442747 1104 72-73 3.2182921807365403 1104 74-75 3.0479949268129864 1104 76-77 3.3015812534694398 1104 78-79 3.5759537042372784 1104 80-81 3.6861372519344187 1104 82-83 3.5981180955804852 1104 84-85 3.750632944250466 1104 86-87 3.990363337726432 1104 88-89 4.076219015864279 1104 90-91 3.728834194263106 1104 92-93 3.766951483879579 1104 94-95 3.795290024800236 1104 96-97 3.365509448664417 1104 98-99 3.3006554962434045 1104 100-101 2.1983874015779206 1105 1 0.601019755853553 1105 2 0.46557789687688 1105 3 0.42398871084746403 1105 4 0.23524356307014216 1105 5 0.3461993911787786 1105 6 0.435406799308943 1105 7 0.43367263140576995 1105 8 0.4309209539075525 1105 9 0.5926258496049428 1105 10-11 0.5603984364524663 1105 12-13 0.5730472298820928 1105 14-15 0.6021677440228643 1105 16-17 0.6937583026739347 1105 18-19 0.6292565447106853 1105 20-21 0.6532455322254904 1105 22-23 0.6516297635600949 1105 24-25 0.7428019959185903 1105 26-27 0.6567877498777506 1105 28-29 0.7554895932760033 1105 30-31 0.8876416443926161 1105 32-33 1.002122988074838 1105 34-35 0.9142526306230536 1105 36-37 1.0571661341360894 1105 38-39 0.9906616752641426 1105 40-41 1.0727288827220605 1105 42-43 1.2956774223357428 1105 44-45 1.2637534494492115 1105 46-47 1.3898018084296169 1105 48-49 1.3480425219577086 1105 50-51 1.422345671752872 1105 52-53 1.487240435311925 1105 54-55 1.610195815718157 1105 56-57 1.5874187884562616 1105 58-59 1.6739481130761398 1105 60-61 1.8556581720974812 1105 62-63 1.899723121939207 1105 64-65 2.0419278906307063 1105 66-67 2.3293574395709413 1105 68-69 2.4808313841272778 1105 70-71 2.617685495256417 1105 72-73 2.5894648860424248 1105 74-75 2.7196587553304425 1105 76-77 2.856331731916331 1105 78-79 2.885357176875498 1105 80-81 3.0464373778001637 1105 82-83 3.4199140719150343 1105 84-85 3.3536865908795797 1105 86-87 3.3326498539375358 1105 88-89 3.4578301647425924 1105 90-91 3.3272382120952084 1105 92-93 3.2808864599740613 1105 94-95 3.3085586528187125 1105 96-97 3.2100977824128165 1105 98-99 2.7573211084198483 1105 100-101 2.054936122391539 1106 1 0.26683302357085736 1106 2 0.2420824112336426 1106 3 0.09000572675023832 1106 4 0.144875058764093 1106 5 0.08746913945538637 1106 6 -0.2358243474743773 1106 7 0.14377151111622766 1106 8 -0.22563316304260894 1106 9 0.15547728267723926 1106 10-11 0.2123519454128271 1106 12-13 0.3989541696885013 1106 14-15 0.5271766946220566 1106 16-17 -0.059488323763858375 1106 18-19 0.31083783893478056 1106 20-21 0.43703908909596834 1106 22-23 0.37242639943669786 1106 24-25 0.5423010878396113 1106 26-27 0.5668007038358738 1106 28-29 0.6498162152873235 1106 30-31 0.4305729493574262 1106 32-33 0.49109975557406926 1106 34-35 0.42983708847712165 1106 36-37 0.6379103032275637 1106 38-39 0.6416120661612226 1106 40-41 0.9146938484494029 1106 42-43 0.4919305387716477 1106 44-45 1.0441073724154748 1106 46-47 1.0996474675517405 1106 48-49 0.8759010178664752 1106 50-51 0.6095472913231106 1106 52-53 0.8735118435178322 1106 54-55 0.8237073147970477 1106 56-57 1.3686986915945027 1106 58-59 1.184885419824571 1106 60-61 1.4841594837671117 1106 62-63 1.5664750634055409 1106 64-65 1.5577841212241772 1106 66-67 1.7149114979353577 1106 68-69 1.8945645325900529 1106 70-71 1.9512886484711487 1106 72-73 2.0696808070428077 1106 74-75 2.323016369198335 1106 76-77 2.2995393662892347 1106 78-79 2.681473593250864 1106 80-81 2.5946638572528435 1106 82-83 2.804345236925027 1106 84-85 2.7681126784587455 1106 86-87 2.59759995841544 1106 88-89 2.4472450233882164 1106 90-91 2.507487657158057 1106 92-93 2.5952428956176217 1106 94-95 2.447777900187239 1106 96-97 2.3475871230628567 1106 98-99 2.3282303591082325 1106 100-101 1.4509509901505036 1107 1 0.15280589612691742 1107 2 0.14511024986076393 1107 3 0.14365170794541626 1107 4 0.09819792890269952 1107 5 0.12692616907341403 1107 6 0.1298719529267558 1107 7 0.1610547913690965 1107 8 0.14860064142375506 1107 9 0.2833208273365315 1107 10-11 0.17167204024276117 1107 12-13 0.23002801909866122 1107 14-15 0.34408421725409255 1107 16-17 0.3188581319529007 1107 18-19 0.311853578769842 1107 20-21 0.3186970302351284 1107 22-23 0.42742150049620165 1107 24-25 0.3905494325101415 1107 26-27 0.29264124094653 1107 28-29 0.3809955851071223 1107 30-31 0.39012301132251537 1107 32-33 0.5691938466377451 1107 34-35 0.5838001887729618 1107 36-37 0.4590834786046756 1107 38-39 0.5580471127393807 1107 40-41 0.5518072588186271 1107 42-43 0.671444785369971 1107 44-45 0.5245739061020416 1107 46-47 0.5929569322386641 1107 48-49 0.6370987437332474 1107 50-51 0.8874366434521761 1107 52-53 0.9565919650594878 1107 54-55 0.9937151167368796 1107 56-57 1.1835670896471626 1107 58-59 1.26082312993044 1107 60-61 1.5175309589990498 1107 62-63 1.608067708754895 1107 64-65 1.58450245921264 1107 66-67 1.7722661899054337 1107 68-69 1.7929932351332454 1107 70-71 2.0462094947336738 1107 72-73 1.9288258334508015 1107 74-75 2.0753288509383694 1107 76-77 2.202710288690927 1107 78-79 2.476167282398933 1107 80-81 2.5830797535399377 1107 82-83 2.633323348103495 1107 84-85 2.595829689538906 1107 86-87 2.6063951165844834 1107 88-89 2.9679810132655327 1107 90-91 2.8282978026551415 1107 92-93 2.6699338656574323 1107 94-95 2.6134180828220526 1107 96-97 2.3919244974539 1107 98-99 2.168260323020128 1107 100-101 1.4759223630646323 1108 1 0.19449515610580903 1108 2 0.1861195434411691 1108 3 0.1889681937789014 1108 4 0.14026627496437527 1108 5 0.054637939599970764 1108 6 0.16282431887272963 1108 7 0.10608923561598971 1108 8 0.1552705724428023 1108 9 0.19195202800887046 1108 10-11 0.18944685309308795 1108 12-13 0.1025133714088966 1108 14-15 0.24979247738188093 1108 16-17 0.28563021588425386 1108 18-19 0.3046390967568797 1108 20-21 0.33854025078714045 1108 22-23 0.34188322041053 1108 24-25 0.36891966284923683 1108 26-27 0.20736599035580383 1108 28-29 0.33904483663362583 1108 30-31 0.4549265540052403 1108 32-33 0.46506320486697206 1108 34-35 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-0.694979908084008 2316 48-49 -0.621996295395526 2316 50-51 -0.6335578719693729 2316 52-53 -0.7184613265741078 2316 54-55 -0.7449932265021317 2316 56-57 -0.7415454551183522 2316 58-59 -0.612598339496575 2316 60-61 -0.8723681279418507 2316 62-63 -0.7155336368972982 2316 64-65 -0.5331492025708435 2316 66-67 -0.41815220776684825 2316 68-69 -0.5621102416415198 2316 70-71 -0.569312401424444 2316 72-73 -0.3143282134747807 2316 74-75 -0.14337726329732803 2316 76-77 -0.09306605760355424 2316 78-79 0.00887103085156582 2316 80-81 0.03897714991209256 2316 82-83 -0.011932402887882887 2316 84-85 0.10939006278116992 2316 86-87 0.05881394703078513 2316 88-89 -0.05725331251705157 2316 90-91 -0.04746068266473458 2316 92-93 0.035513477079915035 2316 94-95 -0.17440531715341123 2316 96-97 -0.13374929659560308 2316 98-99 -0.34022644426288196 2316 100-101 0.07855656733758742 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8967.0 3 3473.0 4 3938.0 5 4572.0 6 5703.0 7 7484.0 8 9720.0 9 12781.0 10 16575.0 11 20894.0 12 26588.0 13 31380.0 14 35592.0 15 41386.0 16 48453.0 17 55875.0 18 55543.0 19 54601.0 20 59550.0 21 65200.0 22 72371.0 23 79838.0 24 88678.0 25 97811.0 26 107919.0 27 118911.0 28 130722.0 29 143470.0 30 157704.0 31 174632.0 32 192562.0 33 213005.0 34 233552.0 35 245130.0 36 225108.0 37 144822.0 38 32352.0 39 397.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.57006431740444 18.03338111096593 13.880176085227692 23.516378486401944 2 15.84039561090243 19.821187361386833 39.27295989172643 25.06545713598431 3 19.18675792643858 23.94561159038316 29.449880428902866 27.4177500542754 4 12.994601775614303 15.930429495091698 35.68786465278363 35.387104076510376 5 14.082963297906447 37.18371804457349 33.33551487314346 15.397803784376604 6 33.3963495124068 34.87542296305159 16.90407901964452 14.824148504897083 7 29.47065310097308 32.68574759522207 19.753496424349912 18.09010287945494 8 27.43917087802584 34.002546477510464 19.33832561007567 19.21995703438802 9 27.429809755059477 16.050636374811482 19.386994399584427 37.13255947054462 10-11 25.57459038671724 26.068794708836478 26.696319036143784 21.660295868302505 12-13 26.4848757098559 23.412056509923566 27.301785136927244 22.80128264329329 14-15 22.99802789364933 24.84039518784243 25.66467517572926 26.496901742778977 16-17 22.587431175367534 28.226487371157994 26.288188801811657 22.89789265166282 18-19 22.637375164176145 26.129771433763704 28.985255123534802 22.247598278525345 20-21 23.234074402067236 25.4900107723725 29.03922663617799 22.236688189382274 22-23 22.921731659665515 25.437503872318807 29.032073125900958 22.60869134211472 24-25 22.668198927307394 25.759014286044753 29.132163263417745 22.44062352323011 26-27 22.214710078048093 26.156735270607367 28.912921141321608 22.71563351002293 28-29 22.305075930538802 26.401758024472706 28.95326269932624 22.33990334566226 30-31 22.337947760435746 25.914777252984244 28.864861506881656 22.882413479698354 32-33 22.179502345004053 26.038268139995786 28.777729829892213 23.00449968510795 34-35 22.85543832948359 26.21313124020389 28.4933535576256 22.438076872686914 36-37 23.094363663309068 25.697972635728856 28.485253617555458 22.722410083406622 38-39 22.46925947027357 25.998203068461308 28.56039155201 22.972145909255122 40-41 22.78460688649026 25.8186065534776 28.684745377467202 22.71204118256494 42-43 22.70798716366101 25.895696734140532 28.32453796001851 23.071778142179948 44-45 22.46884250841738 26.15445301586216 28.496552849670152 22.880151626050306 46-47 22.823929853903046 26.545580276837015 27.775568701349957 22.854921167909982 48-49 22.86519444146635 26.333487900404844 27.716777839872584 23.084539818256218 50-51 22.942327317441087 26.66503024354403 27.47360606978629 22.919036369228593 52-53 23.15654130079896 26.45889394873164 27.471168999867363 22.913395750602042 54-55 22.554157960373633 26.588333429687566 27.64892922919368 23.20857938074512 56-57 22.63549657755712 26.771730930694027 27.585935204258867 23.006837287489994 58-59 22.737125992601612 26.191799140180223 27.637170456925436 23.43390441029273 60-61 22.43708649964173 26.470382249666592 27.947032972333158 23.145498278358524 62-63 22.527543683858077 26.514799371902587 27.931487429957656 23.026169514281683 64-65 22.260691949652063 26.41786713193729 27.76359733080546 23.55784358760518 66-67 22.197958584001164 26.59450009885492 27.754662078873505 23.452879238270413 68-69 22.55304486975665 26.142423887547206 27.925757581513693 23.378773661182457 70-71 22.423857790458214 26.345083089532128 27.535465084490312 23.69559403551935 72-73 22.478354082723026 26.427915300401576 27.36527597935418 23.72845463752122 74-75 22.386138384892895 26.759940797906783 27.21044824442007 23.64347257278025 76-77 22.50029442258734 26.435068546368377 27.281386893082733 23.783250137961552 78-79 22.56698262882609 26.421610853659445 27.250716262567114 23.760690254947352 80-81 22.62229999126172 26.638803281827037 27.009379032059577 23.72951769485167 82-83 22.632746732353816 26.47174162906736 26.9900983622372 23.905413276341626 84-85 22.622459345396884 26.24467918170481 26.992443075296702 24.140418397601607 86-87 22.78428931923312 26.22654218154202 26.80253859273405 24.186629906490804 88-89 22.660105927296648 26.22868494806219 26.72972680986113 24.381482314780033 90-91 22.57530263543736 26.228321968586215 26.76756854986928 24.428806846107143 92-93 22.698711888842617 25.653714636656716 26.977488737431045 24.670084737069622 94-95 22.479520752485865 25.639735398226726 26.964990964466956 24.915752884820453 96-97 22.691534959142228 25.413690534205518 26.825896292321815 25.068878214330436 98-99 22.581325076814334 25.485081228031664 26.42125723275458 25.51233646239942 100-101 23.297125217725775 24.33384139459592 26.692762615775212 25.67627077190309 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1152.0 1 841.5 2 676.0 3 857.5 4 764.0 5 750.5 6 1314.0 7 2542.5 8 3985.5 9 4512.5 10 3750.0 11 3087.5 12 3113.0 13 3217.0 14 3432.0 15 3760.5 16 3987.0 17 4222.0 18 4627.5 19 5134.0 20 5810.0 21 6727.5 22 7897.5 23 9454.0 24 11264.0 25 13432.5 26 16075.0 27 19155.5 28 22409.0 29 25897.0 30 30272.5 31 36832.0 32 44942.0 33 48500.5 34 49048.0 35 52391.0 36 56904.5 37 61409.5 38 65770.5 39 68988.0 40 72795.0 41 79870.5 42 87144.0 43 92981.0 44 98017.5 45 103559.5 46 107850.0 47 109968.0 48 111659.0 49 110734.0 50 109166.0 51 108535.5 52 109071.5 53 111045.0 54 112131.5 55 107998.5 56 100084.5 57 92637.5 58 86167.5 59 76972.5 60 64494.0 61 54143.0 62 46048.0 63 37501.5 64 29487.0 65 23348.0 66 18186.0 67 14467.5 68 11128.0 69 7923.0 70 5917.5 71 4466.0 72 3483.0 73 2745.5 74 2144.0 75 1746.5 76 1262.5 77 788.0 78 485.0 79 301.0 80 173.5 81 106.0 82 67.0 83 41.5 84 23.5 85 16.5 86 10.0 87 9.0 88 7.0 89 5.0 90 4.0 91 3.5 92 3.0 93 1.0 94 1.0 95 1.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.393127908778205 2 0.028111238582493273 3 0.033892045576543 4 0.041753943088450644 5 0.061871151427743715 6 0.05222546204338644 7 0.0362043683741629 8 0.03693109839627201 9 0.0348500078784141 10-11 0.03572538722322735 12-13 0.028160788356727987 14-15 0.026410029667101494 16-17 0.020992587684106317 18-19 0.0258980153333428 20-21 0.03300014964031819 22-23 0.032091737112681804 24-25 0.04059778168964069 26-27 0.03461877559865211 28-29 0.029977613412000757 30-31 0.03268633440349834 32-33 0.0272523758290916 34-35 0.02859021973342882 36-37 0.031067708445164422 38-39 0.014286851571008626 40-41 0.01146251443963004 42-43 0.012668225612674701 44-45 0.01118173238563334 46-47 0.019968559016588935 48-49 0.01735893757356077 50-51 0.010967016697282922 52-53 0.008770310039544024 54-55 0.007729764780615071 56-57 0.008406945028489468 58-59 0.0037327496590149702 60-61 0.006804835661567114 62-63 0.011231282159868052 64-65 0.00672225270450926 66-67 0.0031546689596099967 68-69 0.005797323585461304 70-71 0.012337893784443285 72-73 0.009992537804000253 74-75 0.011198248977044911 76-77 0.004872394466413347 78-79 0.008638177308251458 80-81 0.011479031031041613 82-83 0.012750808569732553 84-85 0.007729764780615071 86-87 0.002312322797619893 88-89 0.01539346319558386 90-91 0.02383344140689647 92-93 0.01015770371811596 94-95 0.019737326736826946 96-97 0.01783791872449632 98-99 0.010488035546347372 100-101 0.12134739710080968 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3027259.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.678752758775246 #Duplication Level Percentage of deduplicated Percentage of total 1 72.41947049870105 33.080310878315835 2 13.816001337806757 12.62195418449166 3 5.267752757317457 7.218731273875821 4 2.6533286405420804 4.848029718363957 5 1.4914622401898532 3.406406745934068 6 0.9292669377383325 2.546865281751209 7 0.6277159995159814 2.0071298761232597 8 0.48119030243589983 1.7584138283911803 9 0.33637375941132264 1.38286204116206 >10 1.7516083747120128 14.504524334609027 >50 0.1335779356550789 4.189654190081712 >100 0.08349069592592756 7.164916976261823 >500 0.00503729902779369 1.631458231281648 >1k 0.0035042080192316818 2.6692314699350694 >5k 1.4600866746798677E-4 0.5451644250027924 >10k+ 7.300433373399338E-5 0.4243465444189218 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12725 0.42034725142447343 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9628 0.3180434842212047 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6720 0.22198298857150972 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3457 0.11419571301959958 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3377 0.11155305839374828 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.3213273129256532E-4 2 0.0 0.0 0.0 0.0 1.3213273129256532E-4 3 0.0 0.0 0.0 0.0 1.3213273129256532E-4 4 0.0 0.0 0.0 0.0 1.3213273129256532E-4 5 0.0 0.0 0.0 0.0 1.3213273129256532E-4 6 3.303318282314133E-5 0.0 0.0 0.0 1.6516591411570665E-4 7 6.606636564628266E-5 0.0 0.0 0.0 1.9819909693884798E-4 8 6.606636564628266E-5 0.0 0.0 0.0 1.9819909693884798E-4 9 6.606636564628266E-5 0.0 0.0 0.0 1.9819909693884798E-4 10-11 6.606636564628266E-5 0.0 0.0 0.0 1.9819909693884798E-4 12-13 9.909954846942399E-5 0.0 0.0 0.0 4.789811509355493E-4 14-15 9.909954846942399E-5 0.0 0.0 0.0 5.450475165818319E-4 16-17 1.3213273129256532E-4 0.0 0.0 0.0 5.945972908165439E-4 18-19 1.9819909693884798E-4 0.0 0.0 1.6516591411570665E-5 5.945972908165439E-4 20-21 2.3123227976198932E-4 0.0 0.0 8.258295705785333E-5 6.276304736396853E-4 22-23 2.3123227976198932E-4 0.0 0.0 3.4684841964298396E-4 6.606636564628266E-4 24-25 2.6426546258513065E-4 0.0 0.0 9.579623018710986E-4 6.771802478743973E-4 26-27 2.807820539967013E-4 0.0 0.0 0.0016516591411570665 6.936968392859679E-4 28-29 3.4684841964298396E-4 0.0 0.0 0.005367892208760466 7.267300221091092E-4 30-31 3.633650110545546E-4 0.0 0.0 0.017012089153917786 7.432466135206799E-4 32-33 3.633650110545546E-4 0.0 0.0 0.04003621758164729 7.597632049322506E-4 34-35 3.9639819387769597E-4 0.0 0.0 0.07534869001958538 7.927963877553919E-4 36-37 4.294313767008373E-4 0.0 0.0 0.13629491232828114 7.927963877553919E-4 38-39 4.9549774234712E-4 0.0 0.0 0.22568270504770155 8.753793448132453E-4 40-41 5.285309251702613E-4 0.0 0.0 0.32314711096738 8.91895936224816E-4 42-43 5.615641079934026E-4 0.0 0.0 0.4253517786221793 9.084125276363866E-4 44-45 5.945972908165439E-4 0.0 0.0 0.5511751719955247 9.579623018710986E-4 46-47 6.111138822281146E-4 0.0 0.0 0.7000557269794226 0.0010240286675173814 48-49 6.276304736396853E-4 0.0 0.0 0.8611420430164713 0.0011231282159868052 50-51 6.276304736396853E-4 0.0 0.0 1.0368290258613486 0.0011891945816330878 52-53 6.276304736396853E-4 3.303318282314133E-5 0.0 1.2063388035182983 0.0012882941301025118 54-55 6.606636564628266E-4 3.303318282314133E-5 0.0 1.394578395835969 0.0012882941301025118 56-57 6.606636564628266E-4 3.303318282314133E-5 0.0 1.6117220231238885 0.0013378439043372238 58-59 6.606636564628266E-4 3.303318282314133E-5 0.0 1.8478927637179376 0.0013543604957487944 60-61 6.606636564628266E-4 3.303318282314133E-5 0.0 2.103965996962929 0.0013708770871603652 62-63 6.606636564628266E-4 3.303318282314133E-5 0.0 2.3545061720850446 0.0013873936785719358 64-65 6.606636564628266E-4 3.303318282314133E-5 0.0 2.6148076527313986 0.0014039102699835064 66-67 7.267300221091092E-4 3.303318282314133E-5 0.0 2.9218841202553203 0.0014534600442182187 68-69 7.267300221091092E-4 3.303318282314133E-5 0.0 3.2619772540109717 0.0014864932270413599 70-71 7.432466135206799E-4 3.303318282314133E-5 0.0 3.6171500357253876 0.0014864932270413599 72-73 7.597632049322506E-4 3.303318282314133E-5 0.0 3.978334856713615 0.0015855927755107839 74-75 7.597632049322506E-4 3.303318282314133E-5 0.0 4.340907071380414 0.001684692323980208 76-77 7.597632049322506E-4 3.303318282314133E-5 0.0 4.735290241105898 0.001717725506803349 78-79 7.597632049322506E-4 3.303318282314133E-5 0.0 5.164407802569915 0.0017342420982149197 80-81 9.579623018710986E-4 3.303318282314133E-5 0.0 5.61104946752161 0.0018003084638612025 82-83 9.579623018710986E-4 3.303318282314133E-5 0.0 6.073051562486064 0.0019159246037421971 84-85 9.909954846942398E-4 3.303318282314133E-5 0.0 6.5343764772026445 0.0019159246037421971 86-87 0.0010240286675173812 3.303318282314133E-5 0.0 7.013572343826544 0.0019489577865653385 88-89 0.0010240286675173812 3.303318282314133E-5 0.0 7.5052052037833565 0.0019985075608000506 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6435 0.0 51.43882 1 GTATCAA 10880 0.0 46.073788 1 TATCAAC 14460 0.0 34.481327 2 ATCAACG 14425 0.0 34.433193 3 TCAACGC 14720 0.0 33.872284 4 CAACGCA 14985 0.0 33.17647 5 AACGCAG 15605 0.0 31.909763 6 GTGGTAT 2330 0.0 28.86312 1 GTACATG 13225 0.0 28.094536 1 TACATGG 13485 0.0 27.457645 2 ACGCAGA 18110 0.0 27.307337 7 CGCAGAG 18385 0.0 26.770123 8 ACATGGG 14075 0.0 25.361115 3 TAAGGTG 1305 0.0 24.765875 5 TGGTATC 2715 0.0 24.684599 2 GCAGAGT 19715 0.0 24.26377 9 GTAAGGT 1250 0.0 23.575369 4 AGGTAAG 1115 0.0 23.01949 2 GAGTACT 11420 0.0 22.752487 12-13 CATGGGA 7485 0.0 21.463558 4 >>END_MODULE