FastQCFastQC Report
Thu 26 May 2016
SRR938492_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938492_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3027259
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128620.42487279747124385No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105100.34717875147121535No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74910.24745157252815173No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA35850.11842396042096168No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA34620.11436087893371528No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69850.051.630611
GTATCAA113550.043.058621
TATCAAC151100.032.2227132
ATCAACG151000.032.1166343
TCAACGC153650.031.5627164
CAACGCA156350.031.0176665
AACGCAG160800.030.1888166
GTACATG137200.026.8051821
TACATGG139600.026.098332
ACGCAGA186350.025.9477447
GTGGTAT26500.025.819541
CGCAGAG187150.025.7606878
GCACCGT6050.025.1232516
ACATGGG143250.024.5368233
GCAGAGT200200.023.5832429
TGGTATC28150.023.2864592
GTAAGGT15500.022.370334
TATATCG1703.152587E-622.3523045
AGGTAAG13900.022.2126982
GAGTACT118000.022.0985812-13
Produced by FastQC (version 0.11.5)