##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938492_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3027259 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.81935308475423 31.0 31.0 34.0 30.0 34.0 2 32.07626668217024 33.0 31.0 34.0 30.0 34.0 3 32.12779976870166 33.0 31.0 34.0 30.0 34.0 4 35.78939165760181 37.0 35.0 37.0 35.0 37.0 5 35.68280282592272 37.0 35.0 37.0 33.0 37.0 6 35.714530867692524 37.0 35.0 37.0 33.0 37.0 7 35.631999442399874 37.0 35.0 37.0 33.0 37.0 8 35.6086436608166 37.0 35.0 37.0 33.0 37.0 9 37.1479176377046 39.0 37.0 39.0 33.0 39.0 10-11 37.080809570637996 39.0 37.0 39.0 33.0 39.0 12-13 36.96286459136796 39.0 37.0 39.0 33.0 39.0 14-15 37.98090830682145 40.0 37.0 41.0 33.0 41.0 16-17 38.06600938340591 40.0 37.0 41.0 33.0 41.0 18-19 38.21208360434307 40.0 37.5 41.0 33.0 41.0 20-21 38.173359299617246 40.0 37.5 41.0 33.0 41.0 22-23 38.049957568876664 40.0 37.0 41.0 33.0 41.0 24-25 37.932486780946064 40.0 37.0 41.0 33.0 41.0 26-27 37.7231986096994 40.0 37.0 41.0 32.0 41.0 28-29 37.57997994225139 39.5 36.5 41.0 32.0 41.0 30-31 37.311485406435324 39.0 36.0 41.0 31.5 41.0 32-33 37.17681506603829 39.0 36.0 41.0 31.0 41.0 34-35 37.03551133219853 39.0 36.0 41.0 31.0 41.0 36-37 36.90376690597005 39.0 35.5 41.0 31.0 41.0 38-39 36.69040293546075 39.0 35.0 41.0 30.0 41.0 40-41 36.4635389637953 39.0 35.0 40.0 30.0 41.0 42-43 36.18541013504296 38.5 35.0 40.0 30.0 41.0 44-45 35.86828943278391 38.0 35.0 40.0 29.0 41.0 46-47 35.4138160956826 38.0 34.5 40.0 27.5 41.0 48-49 34.97236328308876 38.0 34.0 40.0 26.0 41.0 50-51 34.56684264544263 38.0 33.5 40.0 24.5 41.0 52-53 33.97724856049648 37.5 33.0 40.0 23.5 41.0 54-55 33.56388122060253 37.0 32.5 40.0 23.0 41.0 56-57 33.184496106874235 36.5 32.0 39.0 22.0 40.5 58-59 32.66762325258592 36.0 31.0 39.0 19.5 40.0 60-61 32.20614076958727 35.5 31.0 39.0 18.0 40.0 62-63 31.737346391570725 35.0 30.5 38.5 15.5 40.0 64-65 31.114169947137 35.0 29.5 38.0 10.5 40.0 66-67 30.504664120248712 34.0 29.0 37.5 8.0 40.0 68-69 29.818547735757 34.0 28.0 37.0 6.5 39.0 70-71 29.087463279488144 33.5 26.5 36.0 2.0 39.0 72-73 28.546300134874485 33.0 26.0 36.0 2.0 38.5 74-75 27.86832263113265 32.5 26.0 35.0 2.0 38.0 76-77 26.715315405784573 31.0 24.5 34.0 2.0 36.0 78-79 27.23844656172465 32.0 26.0 35.0 2.0 36.0 80-81 26.896466737732055 32.0 25.0 35.0 2.0 36.0 82-83 26.26552402685069 32.0 23.5 34.5 2.0 36.0 84-85 25.54088913436214 31.0 21.0 34.0 2.0 35.0 86-87 24.685969221662237 31.0 18.5 34.0 2.0 35.0 88-89 23.76018867232701 30.0 11.5 34.0 2.0 35.0 90-91 22.95026490960965 29.5 4.5 34.0 2.0 35.0 92-93 21.926497864900227 29.0 2.0 34.0 2.0 35.0 94-95 20.797575793812157 28.5 2.0 33.5 2.0 35.0 96-97 19.447871985845943 26.5 2.0 33.0 2.0 34.0 98-99 17.69561061673283 22.5 2.0 32.5 2.0 34.0 100-101 13.336287215596684 2.0 2.0 28.5 2.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3155588139225465 1101 2 0.30558245623686275 1101 3 0.2972904565100336 1101 4 0.13204700707068184 1101 5 0.12317433589929294 1101 6 0.17515122184611442 1101 7 0.28078393630243426 1101 8 0.3045260144082249 1101 9 0.29874560651584403 1101 10-11 0.2751352438924428 1101 12-13 0.25808658486949554 1101 14-15 0.3708234901687888 1101 16-17 0.41711967498171276 1101 18-19 0.4079039640953539 1101 20-21 0.4438264374023291 1101 22-23 0.36558793926414523 1101 24-25 0.2795756284901145 1101 26-27 0.5016232081037941 1101 28-29 0.3909688742073598 1101 30-31 0.5053376801102942 1101 32-33 0.38533555316341506 1101 34-35 0.24462812317202776 1101 36-37 0.3486134532033347 1101 38-39 0.4536306240494099 1101 40-41 0.49320595493232133 1101 42-43 0.5865223002650879 1101 44-45 0.6398345481204615 1101 46-47 0.585853150321249 1101 48-49 0.6085922819498748 1101 50-51 0.6684045271215027 1101 52-53 0.6224418380455106 1101 54-55 0.700429689116632 1101 56-57 0.7427543840590545 1101 58-59 0.9661052575037772 1101 60-61 0.8081992898655415 1101 62-63 0.888338655497332 1101 64-65 1.0955643607844472 1101 66-67 1.3280498238812832 1101 68-69 1.491629020420664 1101 70-71 1.5832847839770174 1101 72-73 1.6820144692236845 1101 74-75 1.5615917022155408 1101 76-77 1.6692055889825816 1101 78-79 1.6012435237306981 1101 80-81 1.861946261179451 1101 82-83 2.0593117931629727 1101 84-85 2.2898657088822993 1101 86-87 2.259980694407755 1101 88-89 2.0373292650767105 1101 90-91 2.1136043943493235 1101 92-93 2.4789651696938897 1101 94-95 2.1981171186646478 1101 96-97 2.2840735594795163 1101 98-99 2.1283985240703096 1101 100-101 2.033021120965955 1102 1 0.3203403726245604 1102 2 0.3446953763952365 1102 3 0.32661143879844445 1102 4 0.2589232078431891 1102 5 0.2556330338720585 1102 6 0.30598316812533 1102 7 0.3564015442713284 1102 8 0.34755389091952793 1102 9 0.4588384880997083 1102 10-11 0.3978375774867473 1102 12-13 0.41030756097935495 1102 14-15 0.5243146806477128 1102 16-17 0.49511167977262716 1102 18-19 0.5049271181587969 1102 20-21 0.47402144432845716 1102 22-23 0.45127979954110486 1102 24-25 0.4048426637124152 1102 26-27 0.433770785134449 1102 28-29 0.31150424723018943 1102 30-31 0.37495876090785174 1102 32-33 0.407528329925853 1102 34-35 0.40504892280527116 1102 36-37 0.4221943450494905 1102 38-39 0.3967791695884344 1102 40-41 0.44925524053369514 1102 42-43 0.5150044034554924 1102 44-45 0.519288088471896 1102 46-47 0.4054249364234579 1102 48-49 0.561817018953441 1102 50-51 0.8152214752957363 1102 52-53 0.7981640534769738 1102 54-55 0.7965845691443576 1102 56-57 0.8836155808422532 1102 58-59 1.034839929809067 1102 60-61 0.9161630311162696 1102 62-63 1.2063656025508713 1102 64-65 1.1856340340084905 1102 66-67 1.2838627998606178 1102 68-69 1.4307622415414727 1102 70-71 1.5143957146945688 1102 72-73 1.4944270901635264 1102 74-75 1.4320101106682266 1102 76-77 1.483274249565735 1102 78-79 1.615130358302462 1102 80-81 1.842822032395361 1102 82-83 1.762800656654111 1102 84-85 1.84433333820866 1102 86-87 1.7486623729095179 1102 88-89 1.5722838313087983 1102 90-91 2.012814997175571 1102 92-93 2.1786073485140776 1102 94-95 1.9302557488284577 1102 96-97 2.1502948308761027 1102 98-99 2.2970566598749222 1102 100-101 2.031513995167657 1103 1 0.26188005637596845 1103 2 0.2112261000716984 1103 3 0.2533250126973883 1103 4 0.125186876000825 1103 5 0.08448111111113832 1103 6 0.2063078010210333 1103 7 0.20213299044682742 1103 8 0.20362545765817686 1103 9 0.2672132146071746 1103 10-11 0.40634641399675075 1103 12-13 0.34340588373095926 1103 14-15 0.44404567137970474 1103 16-17 0.38144843885206825 1103 18-19 0.3984510865209998 1103 20-21 0.2958932783813992 1103 22-23 0.38511084293374154 1103 24-25 0.3373195389558248 1103 26-27 0.3905710615058595 1103 28-29 0.35589297603815595 1103 30-31 0.3131097249084078 1103 32-33 0.2916970096442526 1103 34-35 0.38182919589024067 1103 36-37 0.3897368132169632 1103 38-39 0.40582858650427767 1103 40-41 0.4691375497876109 1103 42-43 0.47796675302650726 1103 44-45 0.5422090599639802 1103 46-47 0.7028874561388605 1103 48-49 0.7294594388594291 1103 50-51 0.622036437132941 1103 52-53 0.7186277001563113 1103 54-55 0.7076429189637992 1103 56-57 0.9212169073215009 1103 58-59 0.9124089482635895 1103 60-61 0.7740697635289635 1103 62-63 0.7535124817923453 1103 64-65 1.0402798813857395 1103 66-67 0.9773218157662065 1103 68-69 0.8959147310190438 1103 70-71 0.8863143869731935 1103 72-73 0.9603438061052145 1103 74-75 0.9045565704358758 1103 76-77 0.9427856440639282 1103 78-79 1.203939386435156 1103 80-81 1.403011416679714 1103 82-83 1.5954307265362324 1103 84-85 1.608916652672999 1103 86-87 1.366656661751282 1103 88-89 1.4137348847160247 1103 90-91 1.6940959450156434 1103 92-93 1.9387583694450718 1103 94-95 1.6346494202337212 1103 96-97 1.70785816922535 1103 98-99 2.0262181133090564 1103 100-101 1.840677272963621 1104 1 0.11778659335833197 1104 2 0.1719636094701258 1104 3 0.2474495836125854 1104 4 0.2063513442959959 1104 5 0.14376675847969977 1104 6 0.15759187257640406 1104 7 0.16019618151658221 1104 8 0.09014985273568499 1104 9 0.032717318687708996 1104 10-11 0.10288046573435139 1104 12-13 0.23072623261984404 1104 14-15 0.39215853899937514 1104 16-17 0.37670898851299484 1104 18-19 0.33592048908121086 1104 20-21 0.2876225411842839 1104 22-23 0.3053766172801744 1104 24-25 0.2594352122916703 1104 26-27 0.3964573002847871 1104 28-29 0.21779468949478797 1104 30-31 0.23631393444352256 1104 32-33 0.4219661950497269 1104 34-35 0.35153899163505997 1104 36-37 0.38742666147287963 1104 38-39 0.4216460516069773 1104 40-41 0.5269778209427827 1104 42-43 0.45271326569907444 1104 44-45 0.39803236230152095 1104 46-47 0.4739219198459139 1104 48-49 0.5910688101436037 1104 50-51 0.5536747303542953 1104 52-53 0.6189911460946291 1104 54-55 0.6546546390743941 1104 56-57 0.7673900353827463 1104 58-59 0.8961219821123407 1104 60-61 0.8365234920326827 1104 62-63 0.8075896582041544 1104 64-65 0.9699221045314594 1104 66-67 0.8799655066281709 1104 68-69 1.0506920152875772 1104 70-71 1.1938390150596625 1104 72-73 1.51562770470548 1104 74-75 1.557100812111809 1104 76-77 1.5287186287646044 1104 78-79 1.4448295767578365 1104 80-81 1.7740355511234824 1104 82-83 1.88479350707782 1104 84-85 1.9410882241382907 1104 86-87 1.7453506345060497 1104 88-89 1.7755526428815465 1104 90-91 2.2077188019937815 1104 92-93 2.220858476821366 1104 94-95 2.0639760003107313 1104 96-97 2.2517505719320994 1104 98-99 2.4420095150381265 1104 100-101 2.4074338602711194 1105 1 0.14356147540125974 1105 2 0.12026060776275216 1105 3 0.09026188659679235 1105 4 0.10693243571581235 1105 5 0.05035709800311139 1105 6 0.10093164503087593 1105 7 0.08651648179949945 1105 8 0.11095382300354828 1105 9 0.17463487550117662 1105 10-11 0.21722759045362494 1105 12-13 0.19833072250459338 1105 14-15 0.34046720399232555 1105 16-17 0.22707346071820922 1105 18-19 0.2301902321670113 1105 20-21 0.18139981490077872 1105 22-23 0.2224646313161074 1105 24-25 0.22465116964539789 1105 26-27 0.2604657541553337 1105 28-29 0.24798093041663094 1105 30-31 0.3429345625158433 1105 32-33 0.34527547703163464 1105 34-35 0.47516147964865496 1105 36-37 0.46286477972385853 1105 38-39 0.4421881941964898 1105 40-41 0.5663829987141256 1105 42-43 0.44852559336284514 1105 44-45 0.5223435221734505 1105 46-47 0.5847140663617196 1105 48-49 0.5910782332477069 1105 50-51 0.6521872260634254 1105 52-53 0.620153665473687 1105 54-55 0.6101467382503216 1105 56-57 0.6539263266733286 1105 58-59 0.7132480251041358 1105 60-61 0.8141209594612775 1105 62-63 1.0010343187817021 1105 64-65 0.937891515340656 1105 66-67 0.8955430195120222 1105 68-69 1.0374015122059994 1105 70-71 1.1418998748506048 1105 72-73 1.2127514143572036 1105 74-75 1.3139537645722505 1105 76-77 1.3649066017427351 1105 78-79 1.3483871350969459 1105 80-81 1.269732894481205 1105 82-83 1.4997596399929485 1105 84-85 1.632307012910438 1105 86-87 1.7733856031966617 1105 88-89 1.7423462970940093 1105 90-91 1.7435764054291774 1105 92-93 1.7399951185616764 1105 94-95 1.6635383868688969 1105 96-97 2.01976967839747 1105 98-99 1.8307668544022455 1105 100-101 1.7351833313433112 1106 1 0.0036040477058278952 1106 2 0.04938423018416671 1106 3 0.07915468120170033 1106 4 0.022501270705816978 1106 5 0.03984616525303153 1106 6 -0.010479555260751283 1106 7 0.06914906458440129 1106 8 0.05972580079303924 1106 9 -0.06074310016621354 1106 10-11 0.06904797959312958 1106 12-13 0.028303187206681457 1106 14-15 0.06639171952080147 1106 16-17 0.09058007765209197 1106 18-19 0.018641680862174326 1106 20-21 0.05960461762956726 1106 22-23 0.1649474298918321 1106 24-25 0.08984147565824685 1106 26-27 -0.13335800691900346 1106 28-29 0.10111917323499853 1106 30-31 0.15367560703449357 1106 32-33 0.02359159266686106 1106 34-35 0.03749475439624206 1106 36-37 0.02952721519817203 1106 38-39 0.1750987337711578 1106 40-41 0.3072351608534163 1106 42-43 0.14310511048615382 1106 44-45 0.18848975218107 1106 46-47 -0.2873818710426761 1106 48-49 0.07461303643216155 1106 50-51 0.2874073457085231 1106 52-53 0.3104853909539358 1106 54-55 0.060339094764081835 1106 56-57 0.0729250312775136 1106 58-59 0.44367290191134146 1106 60-61 0.44621520438873574 1106 62-63 0.257240501530152 1106 64-65 0.5719659582879117 1106 66-67 0.6002451761182179 1106 68-69 0.6016863430559489 1106 70-71 0.5398151989273892 1106 72-73 0.7059199915699566 1106 74-75 0.6470029570654638 1106 76-77 0.5997804145677996 1106 78-79 0.8618247510478945 1106 80-81 0.873470339231595 1106 82-83 1.101138683222711 1106 84-85 1.0660781119138285 1106 86-87 1.2097901312234285 1106 88-89 1.258183975536408 1106 90-91 1.3638991477625773 1106 92-93 1.3294757234268992 1106 94-95 1.1641844573931124 1106 96-97 1.3456548112894602 1106 98-99 1.4857511989905525 1106 100-101 1.6794591790464253 1107 1 0.03236109703532719 1107 2 -0.0013637105458883525 1107 3 0.02668043731421932 1107 4 0.02229961039682138 1107 5 -0.05419159068096491 1107 6 -0.06926415519025397 1107 7 -0.008036625302537459 1107 8 -0.021327180081051722 1107 9 -0.055383192301832196 1107 10-11 -0.01553694935673633 1107 12-13 0.016474136468865197 1107 14-15 0.13362353093865664 1107 16-17 -6.228632825155955E-4 1107 18-19 -0.025798279946300795 1107 20-21 0.06841661526088672 1107 22-23 0.0425716483031664 1107 24-25 0.08229192114983874 1107 26-27 0.12025503918062697 1107 28-29 0.1175983949915036 1107 30-31 0.11455846594954977 1107 32-33 0.041025498293969065 1107 34-35 0.033351006960906204 1107 36-37 0.1433498929743422 1107 38-39 0.17972504027581948 1107 40-41 0.2100082380450985 1107 42-43 0.052560148295391684 1107 44-45 0.07297620643496572 1107 46-47 0.16241132383102297 1107 48-49 0.29589586460229356 1107 50-51 0.2555713608313894 1107 52-53 0.36193934938463457 1107 54-55 0.3304940715380198 1107 56-57 0.2517237556934191 1107 58-59 0.3257762106693889 1107 60-61 0.36598208837743584 1107 62-63 0.5414082870396193 1107 64-65 0.7040207115412329 1107 66-67 0.7433589104662985 1107 68-69 0.8218734697725871 1107 70-71 0.8774351568514653 1107 72-73 1.0112863872762432 1107 74-75 0.942348323072725 1107 76-77 0.9549938914270761 1107 78-79 1.091089277718499 1107 80-81 1.3853974307651278 1107 82-83 1.5768088628284929 1107 84-85 1.7530071973223436 1107 86-87 1.9081664351453504 1107 88-89 1.980956424605818 1107 90-91 1.9467324627068088 1107 92-93 1.7752559607992637 1107 94-95 1.6809470573399707 1107 96-97 1.7224218700779836 1107 98-99 1.6346711386081658 1107 100-101 1.504354805373179 1108 1 0.010813867204952032 1108 2 0.04928338438917024 1108 3 0.032298057728468166 1108 4 0.040285492136987955 1108 5 0.09226633336428591 1108 6 0.04408980700091547 1108 7 0.08023948266225744 1108 8 0.04519876757155572 1108 9 0.10934184604499819 1108 10-11 0.06704285086412654 1108 12-13 0.06925378324461917 1108 14-15 0.10072447397308082 1108 16-17 -0.06884795643117059 1108 18-19 -0.08359696481809209 1108 20-21 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5 128.0 6 299.0 7 671.0 8 1426.0 9 2684.0 10 4494.0 11 6848.0 12 9982.0 13 14007.0 14 18227.0 15 24257.0 16 30986.0 17 39235.0 18 38365.0 19 33479.0 20 34468.0 21 36723.0 22 40536.0 23 45476.0 24 51936.0 25 59645.0 26 69382.0 27 81741.0 28 96720.0 29 113587.0 30 132682.0 31 156419.0 32 185813.0 33 223021.0 34 272823.0 35 336546.0 36 390390.0 37 352115.0 38 119428.0 39 2641.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.224356888083406 18.89168824002683 14.523499956219984 23.360454915669777 2 16.40002246537689 20.54769267364415 38.49898245057617 24.553302410402793 3 19.2851069396298 23.98254901688743 29.978871718030593 26.753472325452183 4 13.455835790726859 16.62272702798142 35.40810350221108 34.513333679080645 5 14.033887510483932 37.33866137958167 33.486717817584335 15.140733292350056 6 33.33081180037784 35.335760831828395 16.631943285989077 14.701484081804693 7 29.44356594529903 32.79597153728835 19.70174999892642 18.058712518486196 8 27.469686253925584 34.41650312477599 19.14006640334798 18.973744217950443 9 27.14964831128968 16.00749326699372 19.453788271933846 37.389070149782754 10-11 25.66749326701151 26.292497602616756 26.626248365270367 21.413760765101365 12-13 26.39295151158193 23.493348273140818 27.391561144916903 22.72213907036035 14-15 22.88671038718524 24.978586239234897 25.6272258171501 26.50747755642976 16-17 22.5208018210533 28.30752175482838 26.398914001081508 22.772762423036813 18-19 22.592483827779517 26.19391006848109 29.113300183433267 22.100305920306127 20-21 23.136457765919598 25.51070126474147 29.173783941182435 22.179057028156492 22-23 22.87736199644629 25.51532591033671 29.157085667265335 22.450226425951662 24-25 22.702108542189013 25.797334839670853 29.227605617053324 22.27295100108681 26-27 22.22751899570532 26.231037330690455 29.009547406673274 22.531896266930953 28-29 22.336605444445382 26.424423746895076 29.041683203950253 22.197287604709288 30-31 22.385811719446536 25.946227263673176 28.92322724946891 22.744733767411375 32-33 22.191536149463655 26.083449968304578 28.911742039229647 22.813271843002116 34-35 22.939069521281763 26.211447268181452 28.613578384635662 22.23590482590112 36-37 23.138797372316834 25.711132902213063 28.625513856811978 22.524555868658126 38-39 22.44142249116006 26.030737189726604 28.72424705646428 22.803593262649052 40-41 22.77733223539945 25.80146833515175 28.796118798208397 22.625080631240404 42-43 22.728414684887195 25.931581017267856 28.424644919879547 22.9153593779654 44-45 22.531534552157332 26.163677415199015 28.584219593127063 22.72056843951659 46-47 22.875006276310955 26.545822355294696 27.853904658211924 22.725266710182424 48-49 22.84056303078131 26.37770669770905 27.813873870719352 22.967856400790286 50-51 22.978575668616394 26.637975145172582 27.609596668141045 22.773852518069976 52-53 23.304976548091854 26.470629040990545 27.437179970395665 22.78721444052194 54-55 22.703706554345036 26.575327713948493 27.61920932434258 23.101756407363887 56-57 22.731771997667195 26.821016441113304 27.534434208376986 22.912777352842514 58-59 22.783118325851866 26.301251396064888 27.553721039395707 23.361909238687538 60-61 22.5148062983709 26.564938777950616 27.90060249222151 23.019652431456972 62-63 22.5242207554755 26.691885299539948 27.79889332230906 22.985000622675496 64-65 22.249981129778728 26.539921542822686 27.656823926218344 23.553273401180245 66-67 22.23053194954351 26.70146621908655 27.69905742586817 23.368944405501775 68-69 22.53645863011843 26.360016946171132 27.753104526989585 23.35041989672085 70-71 22.396389736190308 26.542220715908588 27.4133721010895 23.648017446811604 72-73 22.517001802789824 26.706642409247326 27.166861700502352 23.609494087460494 74-75 22.38325164777774 27.046826848974597 26.941071114166316 23.628850389081343 76-77 22.397076034789226 26.774732522060386 27.285755860334383 23.542435582816008 78-79 22.49498638867702 26.82801504595411 27.272790336076298 23.40420822929257 80-81 22.49761458931544 27.123023767559832 27.03180255786056 23.347559085264166 82-83 22.52116085719991 27.026189136366813 26.98820059338104 23.464449413052236 84-85 22.46163400884346 26.859926761466024 26.906142687956606 23.772296541733905 86-87 22.627443654537938 26.829298104174832 26.80827173664747 23.734986504639757 88-89 22.41995151389425 27.0292697123041 26.51555416963002 24.035224604171628 90-91 22.302594720443548 27.046924839390808 26.83204150072806 23.818438939437584 92-93 22.36915061014958 26.587402194632453 26.889904515633745 24.153542679584223 94-95 22.122647313616365 26.54699839706401 26.91678854540785 24.413565743911775 96-97 22.319761870391662 26.391646700860417 26.833234288840167 24.45535713990775 98-99 22.118424621084618 26.666020317389428 26.485378357121082 24.730176704404876 100-101 22.230480520655988 26.301342667192557 26.712605929194012 24.755570882957446 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1198.0 1 837.0 2 672.5 3 890.0 4 759.0 5 745.5 6 1385.0 7 2903.0 8 4551.0 9 4951.5 10 4040.5 11 3205.0 12 3090.5 13 3285.5 14 3640.0 15 3946.5 16 4133.5 17 4403.0 18 4707.5 19 5086.5 20 5616.0 21 6515.5 22 7767.5 23 9248.0 24 11036.5 25 13233.5 26 16035.0 27 19257.0 28 22407.0 29 26040.0 30 30590.5 31 37251.0 32 46514.0 33 49856.5 34 49401.0 35 52421.5 36 56930.5 37 61806.5 38 66568.0 39 69877.5 40 73147.5 41 81241.0 42 89136.5 43 94749.5 44 100072.5 45 106701.5 46 110666.0 47 112475.5 48 114803.5 49 112884.5 50 111248.5 51 110499.0 52 109822.5 53 112394.5 54 115437.5 55 109733.0 56 98802.0 57 90752.0 58 84459.0 59 74532.0 60 60742.5 61 50432.0 62 43286.0 63 34438.0 64 26180.5 65 20385.0 66 15415.0 67 12342.0 68 9338.0 69 6222.0 70 4545.5 71 3463.0 72 2746.0 73 2279.0 74 1824.0 75 1480.5 76 1024.5 77 594.0 78 340.0 79 199.5 80 112.0 81 48.5 82 23.5 83 16.5 84 7.0 85 3.0 86 3.5 87 3.0 88 1.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025237351676879974 2 0.012519576289970562 3 0.0012222277644562292 4 0.0 5 2.6426546258513065E-4 6 0.0 7 0.0 8 6.606636564628266E-5 9 2.6426546258513065E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.001618625958333925 26-27 0.008555594351193605 28-29 2.807820539967013E-4 30-31 0.0 32-33 2.6426546258513065E-4 34-35 0.00994298802976554 36-37 9.744788932826693E-4 38-39 0.012288344010208575 40-41 0.011941495590565591 42-43 0.005301825843114184 44-45 7.927963877553919E-4 46-47 9.909954846942399E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 1.6516591411570665E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 8.258295705785333E-5 66-67 5.615641079934026E-4 68-69 8.753793448132453E-4 70-71 3.303318282314133E-5 72-73 2.1471568835041866E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 8.258295705785333E-5 82-83 0.0010075120761058106 84-85 0.005417441982995179 86-87 0.0035510671534876932 88-89 0.0 90-91 0.0011561613988099466 92-93 3.138152368198426E-4 94-95 4.9549774234711996E-5 96-97 0.0 98-99 0.0 100-101 3.303318282314133E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3027259.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.306118189313786 #Duplication Level Percentage of deduplicated Percentage of total 1 69.64027660704755 29.46209772874258 2 14.909032597217623 12.614865902924413 3 5.7882239780286415 7.34631863162099 4 2.9332837179612534 4.963833906194342 5 1.6774610838828117 3.5483433436360317 6 1.1044477508001578 2.8034938247563947 7 0.7448472830035018 2.205811803141478 8 0.5133544051062643 1.737442570834439 9 0.3880962002047042 1.4776959343215417 >10 2.0368367643952685 15.677628787677891 >50 0.1556454698440982 4.536766991699557 >100 0.09725122298309202 7.670634161123305 >500 0.007104900533255803 2.0655183263973806 >1k 0.0039037915016841957 2.870116872909827 >5k 7.807583003368392E-5 0.24743411930865344 >10k+ 1.5615166006736783E-4 0.7719970947112332 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12862 0.42487279747124385 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10510 0.34717875147121535 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7491 0.24745157252815173 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3585 0.11842396042096168 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3462 0.11436087893371528 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.606636564628266E-5 2 3.303318282314133E-5 0.0 0.0 0.0 6.606636564628266E-5 3 3.303318282314133E-5 0.0 0.0 0.0 6.606636564628266E-5 4 3.303318282314133E-5 0.0 0.0 0.0 6.606636564628266E-5 5 3.303318282314133E-5 0.0 0.0 0.0 9.909954846942399E-5 6 3.303318282314133E-5 0.0 0.0 0.0 1.3213273129256532E-4 7 6.606636564628266E-5 0.0 0.0 0.0 1.3213273129256532E-4 8 9.909954846942399E-5 0.0 0.0 0.0 1.3213273129256532E-4 9 9.909954846942399E-5 0.0 0.0 0.0 1.3213273129256532E-4 10-11 1.3213273129256532E-4 0.0 0.0 0.0 1.3213273129256532E-4 12-13 1.3213273129256532E-4 0.0 0.0 0.0 3.9639819387769597E-4 14-15 1.3213273129256532E-4 0.0 0.0 0.0 4.6246455952397863E-4 16-17 1.3213273129256532E-4 0.0 0.0 0.0 5.780806994049733E-4 18-19 1.3213273129256532E-4 0.0 0.0 1.6516591411570665E-5 6.276304736396853E-4 20-21 1.3213273129256532E-4 0.0 0.0 8.258295705785333E-5 8.258295705785332E-4 22-23 1.3213273129256532E-4 0.0 0.0 3.1381523681984266E-4 8.588627534016745E-4 24-25 2.9729864540827195E-4 0.0 0.0 9.9099548469424E-4 8.588627534016745E-4 26-27 2.9729864540827195E-4 0.0 0.0 0.001684692323980208 8.588627534016745E-4 28-29 3.633650110545546E-4 0.0 0.0 0.005483508348641461 8.91895936224816E-4 30-31 3.633650110545546E-4 0.0 0.0 0.017309387799326056 8.91895936224816E-4 32-33 3.633650110545546E-4 0.0 0.0 0.04053171532399441 8.91895936224816E-4 34-35 3.7988160246612526E-4 0.0 0.0 0.07619103618157548 9.249291190479573E-4 36-37 4.6246455952397863E-4 0.0 0.0 0.1378805051037919 9.249291190479573E-4 38-39 4.9549774234712E-4 0.0 0.0 0.22847400899625703 9.249291190479573E-4 40-41 4.9549774234712E-4 0.0 0.0 0.32711109290615703 9.249291190479573E-4 42-43 5.615641079934026E-4 0.0 0.0 0.430752704013763 9.744788932826693E-4 44-45 6.276304736396853E-4 0.0 0.0 0.5574019269576868 0.001040545258928952 46-47 7.432466135206799E-4 0.0 0.0 0.7071413446949865 0.0011066116245752346 48-49 7.927963877553919E-4 0.0 0.0 0.8689709073455558 0.0011561613988099466 50-51 7.927963877553919E-4 0.0 0.0 1.045698435449362 0.0012882941301025118 52-53 7.927963877553919E-4 0.0 0.0 1.2156046113001895 0.0013543604957487944 54-55 7.927963877553919E-4 0.0 0.0 1.4044718340915 0.0014204268613950773 56-57 8.258295705785332E-4 0.0 0.0 1.6219953429818856 0.0014204268613950773 58-59 8.258295705785332E-4 0.0 0.0 1.8590910126949827 0.0014204268613950773 60-61 8.258295705785332E-4 0.0 0.0 2.117278369640655 0.0015195264098645013 62-63 8.258295705785332E-4 0.0 0.0 2.3691233554842848 0.0015525595926876425 64-65 8.258295705785332E-4 0.0 0.0 2.6314894100570845 0.0015525595926876425 66-67 8.91895936224816E-4 0.0 0.0 2.941241565389681 0.0015690761840992133 68-69 8.91895936224816E-4 0.0 0.0 3.2869833734080895 0.0015855927755107839 70-71 8.91895936224816E-4 0.0 0.0 3.650051085817236 0.0015855927755107839 72-73 9.084125276363866E-4 0.0 0.0 4.021162378243817 0.0016351425497454959 74-75 9.249291190479573E-4 0.0 0.0 4.398004927890214 0.001684692323980208 76-77 9.41445710459528E-4 0.0 0.0 4.810523314985602 0.001684692323980208 78-79 9.579623018710986E-4 0.0 0.0 5.260980312553369 0.001684692323980208 80-81 0.0010735784417520932 0.0 0.0 5.734263239451927 0.001684692323980208 82-83 0.001090095033163664 0.0 0.0 6.2274816921842495 0.001717725506803349 84-85 0.001139644807398376 0.0 0.0 6.7236566147792445 0.0017507586896264905 86-87 0.0011726779902215172 0.0 0.0 7.242376684651033 0.0018333416466843437 88-89 0.0011891945816330878 0.0 0.0 7.7855082766291215 0.0018828914209190557 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6985 0.0 51.63061 1 GTATCAA 11355 0.0 43.05862 1 TATCAAC 15110 0.0 32.222713 2 ATCAACG 15100 0.0 32.116634 3 TCAACGC 15365 0.0 31.562716 4 CAACGCA 15635 0.0 31.017666 5 AACGCAG 16080 0.0 30.188816 6 GTACATG 13720 0.0 26.805182 1 TACATGG 13960 0.0 26.09833 2 ACGCAGA 18635 0.0 25.947744 7 GTGGTAT 2650 0.0 25.81954 1 CGCAGAG 18715 0.0 25.760687 8 GCACCGT 605 0.0 25.123251 6 ACATGGG 14325 0.0 24.536823 3 GCAGAGT 20020 0.0 23.583242 9 TGGTATC 2815 0.0 23.286459 2 GTAAGGT 1550 0.0 22.37033 4 TATATCG 170 3.152587E-6 22.352304 5 AGGTAAG 1390 0.0 22.212698 2 GAGTACT 11800 0.0 22.09858 12-13 >>END_MODULE