##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938491_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1208 Sequences flagged as poor quality 0 Sequence length 101 %GC 50 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.9817880794702 27.0 10.0 31.0 2.0 33.0 2 20.966887417218544 27.0 10.0 31.0 2.0 33.0 3 20.730960264900663 27.0 10.0 31.0 2.0 34.0 4 23.35182119205298 32.0 10.0 35.0 2.0 37.0 5 23.1341059602649 30.0 10.0 35.0 2.0 37.0 6 23.25662251655629 31.0 8.0 35.0 2.0 37.0 7 23.162251655629138 30.0 2.0 35.0 2.0 37.0 8 22.56953642384106 30.0 2.0 35.0 2.0 37.0 9 22.889900662251655 30.0 2.0 37.0 2.0 39.0 10-11 22.762831125827816 30.0 2.0 37.0 2.0 39.0 12-13 22.41639072847682 30.0 2.0 37.0 2.0 39.0 14-15 22.397764900662253 28.5 2.0 37.0 2.0 40.0 16-17 21.853476821192054 27.0 2.0 37.0 2.0 40.0 18-19 21.71274834437086 27.0 2.0 37.5 2.0 40.0 20-21 21.235099337748345 27.0 2.0 38.0 2.0 40.0 22-23 21.00496688741722 26.5 2.0 38.0 2.0 40.0 24-25 20.667632450331126 25.0 2.0 38.0 2.0 40.0 26-27 20.36630794701987 24.5 2.0 37.0 2.0 40.0 28-29 20.185844370860927 24.0 2.0 37.0 2.0 40.0 30-31 19.840645695364238 24.0 2.0 37.0 2.0 40.0 32-33 19.4101821192053 21.0 2.0 37.0 2.0 40.0 34-35 18.866721854304636 17.0 2.0 36.5 2.0 40.0 36-37 18.443708609271525 15.5 2.0 36.0 2.0 40.0 38-39 18.41721854304636 15.0 2.0 36.0 2.0 40.0 40-41 18.28559602649007 13.5 2.0 36.0 2.0 40.0 42-43 17.966473509933774 11.5 2.0 35.5 2.0 39.0 44-45 17.28062913907285 7.5 2.0 35.0 2.0 39.0 46-47 16.867549668874172 2.0 2.0 35.0 2.0 39.0 48-49 16.628311258278146 2.0 2.0 35.0 2.0 39.0 50-51 16.217715231788077 2.0 2.0 33.5 2.0 38.5 52-53 16.1783940397351 2.0 2.0 34.0 2.0 38.5 54-55 16.11341059602649 2.0 2.0 34.5 2.0 39.0 56-57 15.746688741721854 2.0 2.0 34.0 2.0 39.0 58-59 15.566639072847682 2.0 2.0 34.0 2.0 38.0 60-61 15.311258278145695 2.0 2.0 34.0 2.0 38.0 62-63 14.894453642384107 2.0 2.0 33.0 2.0 38.0 64-65 14.535596026490065 2.0 2.0 33.0 2.0 38.0 66-67 14.122930463576159 2.0 2.0 32.0 2.0 37.0 68-69 13.498344370860927 2.0 2.0 31.0 2.0 36.0 70-71 13.034768211920529 2.0 2.0 30.5 2.0 36.0 72-73 12.945364238410596 2.0 2.0 30.5 2.0 35.0 74-75 12.554221854304636 2.0 2.0 30.0 2.0 35.0 76-77 12.14114238410596 2.0 2.0 29.5 2.0 34.5 78-79 11.51365894039735 2.0 2.0 28.0 2.0 34.0 80-81 11.136175496688741 2.0 2.0 27.0 2.0 34.0 82-83 10.652317880794701 2.0 2.0 25.0 2.0 34.0 84-85 10.326986754966887 2.0 2.0 24.5 2.0 33.5 86-87 10.332781456953644 2.0 2.0 25.0 2.0 34.0 88-89 9.903145695364238 2.0 2.0 21.5 2.0 33.5 90-91 9.56498344370861 2.0 2.0 20.5 2.0 33.0 92-93 9.078228476821192 2.0 2.0 15.5 2.0 32.0 94-95 8.549668874172186 2.0 2.0 4.5 2.0 32.0 96-97 7.9197019867549665 2.0 2.0 2.0 2.0 31.5 98-99 7.23385761589404 2.0 2.0 2.0 2.0 31.0 100-101 5.795943708609272 2.0 2.0 2.0 2.0 26.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -17.192307692307693 1101 2 -18.13736263736264 1101 3 -17.043956043956044 1101 4 -19.181318681318682 1101 5 -19.153846153846153 1101 6 -19.335164835164836 1101 7 -19.34065934065934 1101 8 -18.516483516483518 1101 9 -19.04945054945055 1101 10-11 -18.5 1101 12-13 -18.071428571428573 1101 14-15 -18.057692307692307 1101 16-17 -18.005494505494507 1101 18-19 -18.796703296703296 1101 20-21 -17.60164835164835 1101 22-23 -16.203296703296704 1101 24-25 -17.34065934065934 1101 26-27 -17.09065934065934 1101 28-29 -17.046703296703296 1101 30-31 -16.456043956043956 1101 32-33 -16.002747252747252 1101 34-35 -15.104395604395606 1101 36-37 -14.543956043956044 1101 38-39 -15.453296703296704 1101 40-41 -15.12912087912088 1101 42-43 -15.206043956043956 1101 44-45 -14.057692307692307 1101 46-47 -13.076923076923077 1101 48-49 -12.5 1101 50-51 -11.648351648351648 1101 52-53 -11.434065934065934 1101 54-55 -11.412087912087912 1101 56-57 -11.159340659340659 1101 58-59 -11.18131868131868 1101 60-61 -10.785714285714286 1101 62-63 -10.23076923076923 1101 64-65 -10.23076923076923 1101 66-67 -8.752747252747254 1101 68-69 -9.087912087912088 1101 70-71 -8.62912087912088 1101 72-73 -8.304945054945055 1101 74-75 -8.07967032967033 1101 76-77 -7.706043956043956 1101 78-79 -6.667582417582418 1101 80-81 -6.697802197802197 1101 82-83 -6.354395604395604 1101 84-85 -6.722527472527473 1101 86-87 -6.656593406593407 1101 88-89 -6.357142857142858 1101 90-91 -6.51923076923077 1101 92-93 -6.038461538461538 1101 94-95 -5.722527472527473 1101 96-97 -4.667582417582418 1101 98-99 -3.4505494505494507 1101 100-101 -2.895604395604396 1102 1 11.807692307692307 1102 2 10.862637362637361 1102 3 11.956043956043956 1102 4 15.818681318681318 1102 5 15.846153846153847 1102 6 15.664835164835164 1102 7 15.65934065934066 1102 8 16.483516483516482 1102 9 17.95054945054945 1102 10-11 16.5 1102 12-13 16.928571428571427 1102 14-15 18.442307692307693 1102 16-17 14.494505494505493 1102 18-19 19.203296703296704 1102 20-21 21.39835164835165 1102 22-23 22.296703296703296 1102 24-25 21.15934065934066 1102 26-27 18.90934065934066 1102 28-29 18.453296703296704 1102 30-31 19.043956043956044 1102 32-33 20.997252747252748 1102 34-35 17.395604395604394 1102 36-37 16.456043956043956 1102 38-39 13.046703296703296 1102 40-41 16.37087912087912 1102 42-43 20.793956043956044 1102 44-45 12.442307692307693 1102 46-47 21.923076923076923 1102 48-49 23.5 1102 50-51 24.85164835164835 1102 52-53 27.065934065934066 1102 54-55 26.587912087912088 1102 56-57 15.340659340659341 1102 58-59 6.318681318681319 1102 60-61 24.214285714285715 1102 62-63 26.26923076923077 1102 64-65 25.76923076923077 1102 66-67 12.747252747252746 1102 68-69 15.412087912087912 1102 70-71 26.37087912087912 1102 72-73 24.695054945054945 1102 74-75 23.92032967032967 1102 76-77 25.293956043956044 1102 78-79 26.332417582417584 1102 80-81 25.302197802197803 1102 82-83 15.645604395604396 1102 84-85 23.277472527472526 1102 86-87 11.843406593406593 1102 88-89 18.642857142857142 1102 90-91 22.98076923076923 1102 92-93 21.46153846153846 1102 94-95 8.777472527472527 1102 96-97 -4.667582417582418 1102 98-99 -3.4505494505494507 1102 100-101 -2.895604395604396 1103 1 12.807692307692307 1103 2 9.862637362637361 1103 3 11.956043956043956 1103 4 13.818681318681318 1103 5 13.846153846153847 1103 6 14.664835164835164 1103 7 15.15934065934066 1103 8 16.483516483516482 1103 9 17.45054945054945 1103 10-11 18.25 1103 12-13 13.928571428571427 1103 14-15 15.942307692307693 1103 16-17 14.994505494505493 1103 18-19 17.453296703296704 1103 20-21 18.14835164835165 1103 22-23 20.296703296703296 1103 24-25 16.65934065934066 1103 26-27 18.40934065934066 1103 28-29 19.453296703296704 1103 30-31 18.293956043956044 1103 32-33 17.747252747252748 1103 34-35 21.395604395604394 1103 36-37 22.706043956043956 1103 38-39 21.296703296703296 1103 40-41 20.62087912087912 1103 42-43 21.043956043956044 1103 44-45 20.442307692307693 1103 46-47 22.923076923076923 1103 48-49 21.25 1103 50-51 22.35164835164835 1103 52-53 19.065934065934066 1103 54-55 23.837912087912088 1103 56-57 26.090659340659343 1103 58-59 24.06868131868132 1103 60-61 22.464285714285715 1103 62-63 26.26923076923077 1103 64-65 27.01923076923077 1103 66-67 26.997252747252745 1103 68-69 18.162087912087912 1103 70-71 16.87087912087912 1103 72-73 19.445054945054945 1103 74-75 21.67032967032967 1103 76-77 23.043956043956044 1103 78-79 16.082417582417584 1103 80-81 14.052197802197803 1103 82-83 15.395604395604396 1103 84-85 19.777472527472526 1103 86-87 24.84340659340659 1103 88-89 18.392857142857142 1103 90-91 24.48076923076923 1103 92-93 19.46153846153846 1103 94-95 11.527472527472527 1103 96-97 7.832417582417582 1103 98-99 11.04945054945055 1103 100-101 5.104395604395604 1104 1 -17.192307692307693 1104 2 -18.13736263736264 1104 3 -17.043956043956044 1104 4 -19.181318681318682 1104 5 -19.153846153846153 1104 6 -19.335164835164836 1104 7 -19.34065934065934 1104 8 -18.516483516483518 1104 9 -19.04945054945055 1104 10-11 -18.5 1104 12-13 -18.071428571428573 1104 14-15 -18.057692307692307 1104 16-17 -18.005494505494507 1104 18-19 -18.796703296703296 1104 20-21 -17.60164835164835 1104 22-23 -16.203296703296704 1104 24-25 -17.34065934065934 1104 26-27 -17.09065934065934 1104 28-29 -17.046703296703296 1104 30-31 -16.456043956043956 1104 32-33 -16.002747252747252 1104 34-35 -15.104395604395606 1104 36-37 -14.543956043956044 1104 38-39 -15.453296703296704 1104 40-41 -15.12912087912088 1104 42-43 -15.206043956043956 1104 44-45 -14.057692307692307 1104 46-47 -13.076923076923077 1104 48-49 -12.5 1104 50-51 -11.648351648351648 1104 52-53 -11.434065934065934 1104 54-55 -11.412087912087912 1104 56-57 -11.159340659340659 1104 58-59 -11.18131868131868 1104 60-61 -10.785714285714286 1104 62-63 -10.23076923076923 1104 64-65 -10.23076923076923 1104 66-67 -8.752747252747254 1104 68-69 -9.087912087912088 1104 70-71 -8.62912087912088 1104 72-73 -8.304945054945055 1104 74-75 -8.07967032967033 1104 76-77 -7.706043956043956 1104 78-79 -6.667582417582418 1104 80-81 -6.697802197802197 1104 82-83 -6.354395604395604 1104 84-85 -6.722527472527473 1104 86-87 -6.656593406593407 1104 88-89 -6.357142857142858 1104 90-91 -6.51923076923077 1104 92-93 -6.038461538461538 1104 94-95 -5.722527472527473 1104 96-97 -4.667582417582418 1104 98-99 -3.4505494505494507 1104 100-101 -2.895604395604396 1105 1 0.8076923076923066 1105 2 2.8626373626373613 1105 3 4.456043956043956 1105 4 -3.6813186813186825 1105 5 4.846153846153847 1105 6 11.664835164835164 1105 7 12.65934065934066 1105 8 11.483516483516482 1105 9 14.950549450549449 1105 10-11 7.0 1105 12-13 1.178571428571427 1105 14-15 -1.0576923076923066 1105 16-17 -4.505494505494507 1105 18-19 -2.7967032967032956 1105 20-21 3.8983516483516496 1105 22-23 0.5467032967032956 1105 24-25 10.65934065934066 1105 26-27 10.90934065934066 1105 28-29 0.20329670329670435 1105 30-31 7.793956043956044 1105 32-33 -16.002747252747252 1105 34-35 -15.104395604395606 1105 36-37 -14.543956043956044 1105 38-39 -15.453296703296704 1105 40-41 -15.12912087912088 1105 42-43 -15.206043956043956 1105 44-45 -14.057692307692307 1105 46-47 -13.076923076923077 1105 48-49 -12.5 1105 50-51 -11.648351648351648 1105 52-53 -11.434065934065934 1105 54-55 -11.412087912087912 1105 56-57 -11.159340659340659 1105 58-59 -11.18131868131868 1105 60-61 -10.785714285714286 1105 62-63 -10.23076923076923 1105 64-65 -10.23076923076923 1105 66-67 -8.752747252747254 1105 68-69 -9.087912087912088 1105 70-71 -8.62912087912088 1105 72-73 -8.304945054945055 1105 74-75 -8.07967032967033 1105 76-77 -7.706043956043956 1105 78-79 -6.667582417582418 1105 80-81 -6.697802197802197 1105 82-83 -6.354395604395604 1105 84-85 -6.722527472527473 1105 86-87 -6.656593406593407 1105 88-89 -6.357142857142858 1105 90-91 -6.51923076923077 1105 92-93 -6.038461538461538 1105 94-95 -5.722527472527473 1105 96-97 -4.667582417582418 1105 98-99 -3.4505494505494507 1105 100-101 -2.895604395604396 1107 1 6.307692307692307 1107 2 11.362637362637361 1107 3 -3.043956043956044 1107 4 -3.1813186813186825 1107 5 4.846153846153847 1107 6 -2.8351648351648358 1107 7 0.15934065934066055 1107 8 0.4835164835164818 1107 9 7.450549450549449 1107 10-11 5.5 1107 12-13 2.678571428571427 1107 14-15 1.9423076923076934 1107 16-17 -9.005494505494507 1107 18-19 1.7032967032967044 1107 20-21 3.3983516483516496 1107 22-23 -1.4532967032967044 1107 24-25 8.90934065934066 1107 26-27 11.15934065934066 1107 28-29 -1.0467032967032956 1107 30-31 -8.956043956043956 1107 32-33 -7.252747252747252 1107 34-35 -6.354395604395606 1107 36-37 -5.793956043956044 1107 38-39 -6.703296703296704 1107 40-41 -7.8791208791208796 1107 42-43 -8.206043956043956 1107 44-45 -14.057692307692307 1107 46-47 -13.076923076923077 1107 48-49 -12.5 1107 50-51 -11.648351648351648 1107 52-53 -11.434065934065934 1107 54-55 -11.412087912087912 1107 56-57 -11.159340659340659 1107 58-59 -11.18131868131868 1107 60-61 -10.785714285714286 1107 62-63 -10.23076923076923 1107 64-65 -10.23076923076923 1107 66-67 -8.752747252747254 1107 68-69 -9.087912087912088 1107 70-71 -8.62912087912088 1107 72-73 -8.304945054945055 1107 74-75 -8.07967032967033 1107 76-77 -7.706043956043956 1107 78-79 -6.667582417582418 1107 80-81 -6.697802197802197 1107 82-83 -6.354395604395604 1107 84-85 -6.722527472527473 1107 86-87 -6.656593406593407 1107 88-89 -6.357142857142858 1107 90-91 -6.51923076923077 1107 92-93 -6.038461538461538 1107 94-95 -5.722527472527473 1107 96-97 -4.667582417582418 1107 98-99 -3.4505494505494507 1107 100-101 -2.895604395604396 1108 1 13.807692307692307 1108 2 13.862637362637361 1108 3 14.956043956043956 1108 4 15.818681318681318 1108 5 15.846153846153847 1108 6 15.664835164835164 1108 7 15.65934065934066 1108 8 16.483516483516482 1108 9 17.95054945054945 1108 10-11 17.5 1108 12-13 17.928571428571427 1108 14-15 12.942307692307693 1108 16-17 10.494505494505493 1108 18-19 14.703296703296704 1108 20-21 17.89835164835165 1108 22-23 10.796703296703296 1108 24-25 18.15934065934066 1108 26-27 17.40934065934066 1108 28-29 19.953296703296704 1108 30-31 21.043956043956044 1108 32-33 18.997252747252748 1108 34-35 21.895604395604394 1108 36-37 20.956043956043956 1108 38-39 19.046703296703296 1108 40-41 13.37087912087912 1108 42-43 15.293956043956044 1108 44-45 18.442307692307693 1108 46-47 24.423076923076923 1108 48-49 12.0 1108 50-51 2.851648351648352 1108 52-53 14.565934065934066 1108 54-55 17.587912087912088 1108 56-57 18.840659340659343 1108 58-59 24.81868131868132 1108 60-61 23.214285714285715 1108 62-63 22.76923076923077 1108 64-65 19.26923076923077 1108 66-67 -4.252747252747254 1108 68-69 14.412087912087912 1108 70-71 17.37087912087912 1108 72-73 -8.304945054945055 1108 74-75 -8.07967032967033 1108 76-77 -7.706043956043956 1108 78-79 -6.667582417582418 1108 80-81 -6.697802197802197 1108 82-83 -6.354395604395604 1108 84-85 -6.722527472527473 1108 86-87 -6.656593406593407 1108 88-89 -6.357142857142858 1108 90-91 -6.51923076923077 1108 92-93 -6.038461538461538 1108 94-95 -5.722527472527473 1108 96-97 -4.667582417582418 1108 98-99 -3.4505494505494507 1108 100-101 -2.895604395604396 1109 1 10.807692307692307 1109 2 10.862637362637361 1109 3 11.956043956043956 1109 4 15.818681318681318 1109 5 10.846153846153847 1109 6 13.664835164835164 1109 7 15.65934065934066 1109 8 14.483516483516482 1109 9 17.95054945054945 1109 10-11 7.0 1109 12-13 14.928571428571427 1109 14-15 17.442307692307693 1109 16-17 11.994505494505493 1109 18-19 13.703296703296704 1109 20-21 13.89835164835165 1109 22-23 0.7967032967032956 1109 24-25 -4.8406593406593394 1109 26-27 7.9093406593406606 1109 28-29 4.453296703296704 1109 30-31 -11.456043956043956 1109 32-33 3.497252747252748 1109 34-35 10.895604395604394 1109 36-37 12.456043956043956 1109 38-39 4.546703296703296 1109 40-41 11.87087912087912 1109 42-43 -0.7060439560439562 1109 44-45 -14.057692307692307 1109 46-47 -13.076923076923077 1109 48-49 -12.5 1109 50-51 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4.450549450549449 1110 10-11 11.0 1110 12-13 5.928571428571427 1110 14-15 2.6923076923076934 1110 16-17 8.244505494505493 1110 18-19 13.453296703296704 1110 20-21 15.14835164835165 1110 22-23 4.046703296703296 1110 24-25 12.40934065934066 1110 26-27 -3.3406593406593394 1110 28-29 1.4532967032967044 1110 30-31 0.0439560439560438 1110 32-33 -10.002747252747252 1110 34-35 -2.604395604395606 1110 36-37 3.956043956043956 1110 38-39 2.5467032967032956 1110 40-41 -5.3791208791208796 1110 42-43 -4.706043956043956 1110 44-45 -0.0576923076923066 1110 46-47 4.673076923076923 1110 48-49 3.5 1110 50-51 4.851648351648352 1110 52-53 -3.6840659340659343 1110 54-55 -0.9120879120879124 1110 56-57 2.8406593406593412 1110 58-59 -4.181318681318681 1110 60-61 -3.5357142857142865 1110 62-63 4.01923076923077 1110 64-65 4.26923076923077 1110 66-67 6.747252747252746 1110 68-69 8.162087912087912 1110 70-71 -1.1291208791208796 1110 72-73 6.195054945054945 1110 74-75 8.67032967032967 1110 76-77 10.543956043956044 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82-83 -6.354395604395604 2315 84-85 -6.722527472527473 2315 86-87 -6.656593406593407 2315 88-89 -6.357142857142858 2315 90-91 -6.51923076923077 2315 92-93 -6.038461538461538 2315 94-95 -5.722527472527473 2315 96-97 -4.667582417582418 2315 98-99 -3.4505494505494507 2315 100-101 -2.895604395604396 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 293.0 3 46.0 4 50.0 5 31.0 6 37.0 7 39.0 8 23.0 9 32.0 10 20.0 11 23.0 12 20.0 13 24.0 14 20.0 15 16.0 16 10.0 17 13.0 18 19.0 19 19.0 20 21.0 21 20.0 22 7.0 23 17.0 24 25.0 25 12.0 26 14.0 27 20.0 28 17.0 29 33.0 30 29.0 31 18.0 32 40.0 33 41.0 34 49.0 35 41.0 36 37.0 37 26.0 38 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.294701986754966 17.466887417218544 15.894039735099339 23.344370860927153 2 19.221209610604806 20.878210439105217 35.87406793703397 24.026512013256006 3 23.1980115990058 22.700911350455673 26.67771333885667 27.42336371168186 4 17.06710853355427 14.91300745650373 35.376967688483845 32.64291632145816 5 16.32145816072908 36.37116818558409 30.4059652029826 16.901408450704224 6 33.88566694283347 33.05716652858327 16.23860811930406 16.818558409279206 7 32.94701986754967 31.043046357615893 18.874172185430464 17.135761589403973 8 30.629139072847682 31.125827814569533 19.453642384105958 18.79139072847682 9 30.4635761589404 17.880794701986755 19.453642384105958 32.20198675496689 10-11 29.730848861283643 25.424430641821942 23.975155279503106 20.869565217391305 12-13 29.606625258799173 23.726708074534162 24.306418219461698 22.36024844720497 14-15 26.572847682119203 24.544701986754966 24.296357615894042 24.58609271523179 16-17 26.903973509933778 26.614238410596023 24.917218543046356 21.564569536423843 18-19 25.507246376811594 27.32919254658385 25.341614906832298 21.821946169772257 20-21 27.464788732394368 25.144987572493786 25.22783761391881 22.16238608119304 22-23 26.418219461697724 25.507246376811594 25.051759834368532 23.022774327122153 24-25 26.293995859213247 27.246376811594203 25.631469979296067 20.828157349896482 26-27 27.009113504556755 25.724937862468934 24.647887323943664 22.618061309030654 28-29 25.476387738193868 26.09776304888152 25.22783761391881 23.1980115990058 30-31 26.056338028169012 25.476387738193868 25.310687655343827 23.15658657829329 32-33 26.25258799171843 26.749482401656316 23.892339544513458 23.1055900621118 34-35 28.044739022369512 24.8135874067937 24.44076222038111 22.700911350455673 36-37 25.724937862468934 24.3993371996686 24.31648715824358 25.559237779618886 38-39 26.945364238410598 26.44867549668874 23.965231788079468 22.64072847682119 40-41 29.304635761589402 24.503311258278146 24.130794701986755 22.061258278145697 42-43 27.442052980132452 25.165562913907287 24.296357615894042 23.096026490066226 44-45 27.566225165562912 26.40728476821192 24.2135761589404 21.812913907284766 46-47 28.683774834437088 25.91059602649007 23.137417218543046 22.2682119205298 48-49 28.14569536423841 25.37251655629139 23.137417218543046 23.344370860927153 50-51 27.11092715231788 25.33112582781457 24.875827814569536 22.68211920529801 52-53 28.352649006622517 24.751655629139073 24.420529801324502 22.475165562913908 54-55 26.945364238410598 25.08278145695364 25.45529801324503 22.516556291390728 56-57 26.77980132450331 26.03476821192053 24.62748344370861 22.557947019867548 58-59 27.77317880794702 25.124172185430467 25.579470198675498 21.52317880794702 60-61 27.566225165562912 24.089403973509935 24.62748344370861 23.716887417218544 62-63 25.82781456953642 25.99337748344371 25.04139072847682 23.137417218543046 64-65 26.614238410596023 25.124172185430467 23.799668874172188 24.461920529801322 66-67 27.938741721854303 25.91059602649007 24.089403973509935 22.061258278145697 68-69 26.3658940397351 24.917218543046356 24.62748344370861 24.089403973509935 70-71 27.69039735099338 25.248344370860927 23.22019867549669 23.841059602649008 72-73 25.74503311258278 24.668874172185433 24.917218543046356 24.668874172185433 74-75 28.311258278145697 24.668874172185433 24.089403973509935 22.930463576158942 76-77 28.47682119205298 23.427152317880797 23.882450331125828 24.2135761589404 78-79 25.206953642384107 25.579470198675498 24.668874172185433 24.544701986754966 80-81 27.11092715231788 25.620860927152318 23.013245033112582 24.25496688741722 82-83 26.945364238410598 25.0 23.178807947019866 24.875827814569536 84-85 28.311258278145697 25.165562913907287 22.930463576158942 23.59271523178808 86-87 27.19370860927152 23.67549668874172 23.6341059602649 25.496688741721858 88-89 25.41390728476821 25.41390728476821 24.751655629139073 24.420529801324502 90-91 27.1523178807947 24.130794701986755 23.385761589403973 25.33112582781457 92-93 26.625258799171842 24.637681159420293 24.265010351966872 24.472049689440993 94-95 27.566225165562912 24.834437086092713 23.26158940397351 24.337748344370862 96-97 27.11092715231788 24.62748344370861 23.509933774834437 24.751655629139073 98-99 26.490066225165563 25.08278145695364 22.475165562913908 25.95198675496689 100-101 26.26346313173157 24.026512013256006 23.446561723280862 26.26346313173157 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.5 8 2.0 9 1.0 10 0.5 11 0.5 12 0.5 13 1.0 14 1.0 15 1.0 16 1.0 17 2.0 18 2.5 19 1.5 20 1.5 21 2.0 22 1.0 23 1.0 24 2.5 25 1.5 26 2.0 27 4.5 28 5.5 29 6.5 30 5.5 31 9.5 32 11.5 33 9.0 34 9.5 35 13.0 36 16.5 37 16.5 38 18.5 39 22.0 40 24.0 41 27.0 42 28.0 43 30.0 44 29.0 45 27.5 46 32.0 47 42.0 48 47.5 49 39.5 50 41.0 51 47.0 52 44.0 53 47.5 54 49.0 55 42.0 56 42.0 57 38.5 58 37.0 59 45.0 60 42.5 61 34.5 62 30.0 63 23.0 64 21.0 65 17.0 66 16.5 67 19.0 68 14.0 69 11.5 70 9.0 71 4.5 72 5.5 73 7.0 74 5.0 75 3.5 76 2.0 77 1.0 78 0.0 79 1.0 80 2.0 81 1.5 82 1.0 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.08278145695364239 3 0.08278145695364239 4 0.08278145695364239 5 0.08278145695364239 6 0.08278145695364239 7 0.0 8 0.0 9 0.0 10-11 0.041390728476821195 12-13 0.041390728476821195 14-15 0.0 16-17 0.0 18-19 0.041390728476821195 20-21 0.08278145695364239 22-23 0.041390728476821195 24-25 0.041390728476821195 26-27 0.08278145695364239 28-29 0.08278145695364239 30-31 0.08278145695364239 32-33 0.041390728476821195 34-35 0.08278145695364239 36-37 0.08278145695364239 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.041390728476821195 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.08278145695364239 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1208.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.33774834437085 #Duplication Level Percentage of deduplicated Percentage of total 1 99.41666666666666 98.75827814569537 2 0.5 0.9933774834437087 3 0.08333333333333334 0.24834437086092717 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3 0.24834437086092717 No Hit TCCTGATACAATACTCAGAACGAATGCCATAAGGGCCCACATCACGCCTC 2 0.16556291390728478 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2 0.16556291390728478 No Hit GTGAAGGCGACAGCAGTTGGTTGGAGCAAACATCCCCCAAAGTTCTACAA 2 0.16556291390728478 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2 0.16556291390728478 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 2 0.16556291390728478 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2 0.16556291390728478 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.08278145695364239 0.0 42-43 0.0 0.0 0.0 0.16556291390728478 0.0 44-45 0.0 0.0 0.0 0.33112582781456956 0.0 46-47 0.0 0.0 0.0 0.4966887417218543 0.0 48-49 0.0 0.0 0.0 0.4966887417218543 0.0 50-51 0.0 0.0 0.0 0.7036423841059603 0.0 52-53 0.0 0.0 0.0 0.7450331125827815 0.0 54-55 0.0 0.0 0.0 0.9519867549668874 0.0 56-57 0.0 0.0 0.0 1.076158940397351 0.0 58-59 0.0 0.0 0.0 1.2417218543046358 0.0 60-61 0.0 0.0 0.0 1.283112582781457 0.0 62-63 0.0 0.0 0.0 1.4486754966887418 0.0 64-65 0.0 0.0 0.0 1.5728476821192054 0.0 66-67 0.0 0.0 0.0 1.7384105960264902 0.0 68-69 0.0 0.0 0.0 1.8625827814569536 0.0 70-71 0.0 0.0 0.0 1.903973509933775 0.0 72-73 0.0 0.0 0.0 2.193708609271523 0.0 74-75 0.0 0.0 0.0 2.4834437086092715 0.0 76-77 0.0 0.0 0.0 2.524834437086093 0.0 78-79 0.0 0.0 0.0 2.9387417218543046 0.0 80-81 0.0 0.0 0.0 3.145695364238411 0.0 82-83 0.0 0.0 0.0 3.3940397350993377 0.0 84-85 0.0 0.0 0.0 3.642384105960265 0.0 86-87 0.0 0.0 0.0 3.80794701986755 0.0 88-89 0.0 0.0 0.0 3.890728476821192 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE