FastQCFastQC Report
Thu 26 May 2016
SRR938491_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938491_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1208
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG120.9933774834437087RNA PCR Primer, Index 33 (96% over 29bp)
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG100.8278145695364238RNA PCR Primer, Index 31 (96% over 29bp)
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG80.6622516556291391RNA PCR Primer, Index 39 (96% over 30bp)
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG70.5794701986754967RNA PCR Primer, Index 17 (96% over 28bp)
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGA50.4139072847682119RNA PCR Primer, Index 33 (96% over 29bp)
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG50.4139072847682119RNA PCR Primer, Index 23 (96% over 30bp)
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG40.33112582781456956RNA PCR Primer, Index 46 (96% over 28bp)
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG40.33112582781456956RNA PCR Primer, Index 26 (100% over 29bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.33112582781456956No Hit
TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC40.33112582781456956No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.33112582781456956No Hit
CAGCTGTTCTCATCATGATCTTTATAATTTGAGAGGCCTTTGCTTCAAAA30.24834437086092717No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG30.24834437086092717TruSeq Adapter, Index 3 (96% over 28bp)
CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT30.24834437086092717No Hit
GTGCTCGCCCACGTCGCTGTCGTAGCGCACGTACTCCTCCCGGTTGTAGA30.24834437086092717No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.24834437086092717No Hit
GTCCCTCACCCTCCCAAAAGCCACCCCCACTCCTAAGAGGAGGATGGTCG30.24834437086092717No Hit
GTTCTACAAATGTGGCTGAGGACTTTGTACATTGTTTTGTTTTTTTTTTT20.16556291390728478No Hit
GAACTGCTACGTAACACAGTTCCACCCGCCTCACATTGAAATCCAAATGC20.16556291390728478No Hit
CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG20.16556291390728478No Hit
ATGCTACACATTGGGAGCACATCCATGTCGGGTTGGAAAAGACAGTCCTC20.16556291390728478No Hit
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGGATGCCGTCTTCTGCTTG20.16556291390728478Illumina Paired End PCR Primer 2 (96% over 25bp)
AAGTAGAGATGTCAGATATGTCCTTCAGCAAGGACTGGTCTTTCTATATC20.16556291390728478No Hit
CTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTGA20.16556291390728478RNA PCR Primer, Index 23 (96% over 30bp)
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGA20.16556291390728478RNA PCR Primer, Index 10 (96% over 28bp)
GGTATCAACGCAGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT20.16556291390728478RNA PCR Primer, Index 31 (95% over 22bp)
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGA20.16556291390728478RNA PCR Primer, Index 39 (96% over 30bp)
TCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAA20.16556291390728478TruSeq Adapter, Index 3 (96% over 28bp)
GGATTTAAAAACTGGAACGGTGAAGGCGACAGCAGTTGGTTGGAGCAAAC20.16556291390728478No Hit
CTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTTATA20.16556291390728478No Hit
GAGCATATGGGGCGACGCTCGTCGAAATGCTGCTGTATTTATGAGAAGCC20.16556291390728478No Hit
TCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAA20.16556291390728478Illumina PCR Primer Index 11 (96% over 29bp)
CTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGA20.16556291390728478RNA PCR Primer, Index 31 (96% over 29bp)
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG20.16556291390728478No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC20.16556291390728478No Hit
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGA20.16556291390728478RNA PCR Primer, Index 1 (96% over 28bp)
GTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTAAATTATGTACTG20.16556291390728478No Hit
GTCGTAGCGCACGTACTCCTCCCGGTTGTAGATGTATCTGGTCACATCTC20.16556291390728478No Hit
CGCCACCCACCGGAGAATGGGAAGCCGAACATACTGAACTGCTACGTAAC20.16556291390728478No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)