##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938491_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1208 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.637417218543046 31.0 30.0 31.0 28.0 34.0 2 30.91639072847682 31.0 31.0 31.0 28.0 34.0 3 30.91390728476821 31.0 30.0 34.0 27.0 34.0 4 34.716887417218544 35.0 35.0 37.0 32.0 37.0 5 34.466059602649004 35.0 35.0 37.0 30.0 37.0 6 34.234271523178805 35.0 35.0 37.0 30.0 37.0 7 34.29718543046358 35.0 35.0 37.0 30.0 37.0 8 34.227649006622514 35.0 35.0 37.0 30.0 37.0 9 35.57864238410596 37.0 35.0 39.0 30.0 39.0 10-11 35.411009933774835 37.0 34.5 39.0 30.0 39.0 12-13 35.20198675496689 37.0 34.0 39.0 30.0 39.0 14-15 35.891970198675494 38.0 34.0 40.0 29.0 41.0 16-17 35.69619205298013 38.0 34.0 40.0 28.5 41.0 18-19 35.9635761589404 38.0 34.0 40.0 28.5 41.0 20-21 35.89362582781457 38.0 34.0 40.0 28.5 41.0 22-23 35.75496688741722 38.0 34.0 40.0 27.0 41.0 24-25 35.4271523178808 38.0 33.5 40.0 27.0 41.0 26-27 35.03187086092716 38.0 33.0 40.0 26.5 41.0 28-29 34.234271523178805 37.0 32.0 40.0 24.0 41.0 30-31 34.22475165562914 37.0 32.0 39.5 25.0 41.0 32-33 33.86961920529801 37.0 32.0 39.5 23.5 41.0 34-35 34.26448675496689 37.0 32.5 39.5 24.0 41.0 36-37 34.21605960264901 37.0 32.5 40.0 24.0 41.0 38-39 33.838990066225165 37.0 31.5 40.0 23.5 41.0 40-41 33.42756622516556 37.0 31.0 40.0 22.0 41.0 42-43 33.237168874172184 37.0 31.0 39.0 22.0 40.5 44-45 33.06829470198676 36.0 31.0 39.0 21.5 41.0 46-47 32.12210264900662 36.0 30.0 39.0 18.0 40.0 48-49 31.822433774834437 36.0 30.0 39.0 15.0 40.0 50-51 31.036009933774835 35.0 28.5 39.0 8.5 40.0 52-53 30.01862582781457 34.0 26.5 38.0 4.5 40.0 54-55 29.449917218543046 34.0 26.5 38.0 2.0 40.0 56-57 28.206539735099337 34.0 23.5 38.0 2.0 40.0 58-59 27.35389072847682 33.0 22.5 37.5 2.0 40.0 60-61 26.966059602649008 33.0 21.5 37.0 2.0 39.0 62-63 26.18543046357616 32.0 19.0 36.5 2.0 39.0 64-65 25.459023178807946 31.5 15.5 36.0 2.0 39.0 66-67 24.91680463576159 31.0 10.0 36.0 2.0 38.5 68-69 24.581953642384107 30.5 8.0 35.0 2.0 38.0 70-71 24.15521523178808 30.5 7.5 35.0 2.0 37.5 72-73 23.399006622516556 29.5 2.0 34.5 2.0 37.0 74-75 23.10140728476821 30.0 2.0 34.0 2.0 36.5 76-77 21.813741721854306 28.5 2.0 32.5 2.0 35.0 78-79 22.37003311258278 29.5 2.0 34.0 2.0 35.5 80-81 21.95819536423841 29.0 2.0 34.0 2.0 35.0 82-83 21.276490066225165 29.0 2.0 34.0 2.0 35.0 84-85 20.549254966887418 27.5 2.0 33.5 2.0 35.0 86-87 20.33609271523179 28.0 2.0 33.0 2.0 35.0 88-89 19.394867549668874 26.0 2.0 33.0 2.0 34.5 90-91 18.63948675496689 24.5 2.0 32.5 2.0 34.5 92-93 17.852235099337747 24.0 2.0 32.0 2.0 34.0 94-95 17.016556291390728 19.0 2.0 32.0 2.0 34.0 96-97 16.130380794701985 11.0 2.0 31.5 2.0 34.0 98-99 14.914735099337749 2.0 2.0 31.0 2.0 34.0 100-101 12.322433774834437 2.0 2.0 28.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -14.291208791208792 1101 2 -4.9010989010989015 1101 3 -2.73076923076923 1101 4 0.8626373626373649 1101 5 1.0494505494505475 1101 6 -7.340659340659343 1101 7 -8.857142857142854 1101 8 0.9615384615384599 1101 9 -10.329670329670328 1101 10-11 1.4945054945054963 1101 12-13 -1.7527472527472554 1101 14-15 -6.818681318681321 1101 16-17 -5.310439560439562 1101 18-19 1.521978021978022 1101 20-21 0.7857142857142847 1101 22-23 -3.043956043956044 1101 24-25 -3.008241758241759 1101 26-27 0.3653846153846132 1101 28-29 -16.978021978021978 1101 30-31 -11.208791208791212 1101 32-33 -2.2912087912087884 1101 34-35 -6.321428571428569 1101 36-37 2.5934065934065913 1101 38-39 -0.9148351648351678 1101 40-41 -6.618131868131869 1101 42-43 2.7747252747252773 1101 44-45 -30.10989010989011 1101 46-47 -29.64010989010989 1101 48-49 -28.458791208791208 1101 50-51 -27.65934065934066 1101 52-53 -26.060439560439562 1101 54-55 -25.74175824175824 1101 56-57 -24.708791208791208 1101 58-59 -23.71978021978022 1101 60-61 -23.73076923076923 1101 62-63 -22.162087912087912 1101 64-65 -21.541208791208792 1101 66-67 -21.48076923076923 1101 68-69 -20.923076923076923 1101 70-71 -21.043956043956044 1101 72-73 -20.03846153846154 1101 74-75 -19.225274725274726 1101 76-77 -18.417582417582416 1101 78-79 -19.642857142857142 1101 80-81 -19.431318681318682 1101 82-83 -17.89835164835165 1101 84-85 -17.843406593406595 1101 86-87 -18.052197802197803 1101 88-89 -17.29945054945055 1101 90-91 -16.64835164835165 1101 92-93 -16.74175824175824 1101 94-95 -15.384615384615383 1101 96-97 -13.714285714285714 1101 98-99 -12.659340659340659 1101 100-101 -10.37087912087912 1102 1 0.708791208791208 1102 2 3.0989010989010985 1102 3 3.26923076923077 1102 4 0.8626373626373649 1102 5 1.0494505494505475 1102 6 2.659340659340657 1102 7 3.142857142857146 1102 8 2.96153846153846 1102 9 3.6703296703296715 1102 10-11 2.4945054945054963 1102 12-13 1.7472527472527446 1102 14-15 4.181318681318679 1102 16-17 2.689560439560438 1102 18-19 3.021978021978022 1102 20-21 1.2857142857142847 1102 22-23 2.456043956043956 1102 24-25 2.991758241758241 1102 26-27 1.8653846153846132 1102 28-29 4.521978021978022 1102 30-31 -0.7087912087912116 1102 32-33 5.708791208791212 1102 34-35 4.678571428571431 1102 36-37 4.093406593406591 1102 38-39 5.085164835164832 1102 40-41 -0.1181318681318686 1102 42-43 4.774725274725277 1102 44-45 7.8901098901098905 1102 46-47 6.3598901098901095 1102 48-49 8.041208791208792 1102 50-51 1.3406593406593394 1102 52-53 8.939560439560438 1102 54-55 11.258241758241759 1102 56-57 11.791208791208792 1102 58-59 9.280219780219781 1102 60-61 0.76923076923077 1102 62-63 9.837912087912088 1102 64-65 15.458791208791208 1102 66-67 13.51923076923077 1102 68-69 14.576923076923077 1102 70-71 15.956043956043956 1102 72-73 15.96153846153846 1102 74-75 9.274725274725274 1102 76-77 12.582417582417584 1102 78-79 12.357142857142858 1102 80-81 13.568681318681318 1102 82-83 12.60164835164835 1102 84-85 14.156593406593405 1102 86-87 11.947802197802197 1102 88-89 -17.29945054945055 1102 90-91 -16.64835164835165 1102 92-93 -16.74175824175824 1102 94-95 -15.384615384615383 1102 96-97 -13.714285714285714 1102 98-99 -12.659340659340659 1102 100-101 -10.37087912087912 1103 1 0.20879120879120805 1103 2 1.0989010989010985 1103 3 1.26923076923077 1103 4 1.862637362637365 1103 5 2.0494505494505475 1103 6 1.659340659340657 1103 7 1.142857142857146 1103 8 1.96153846153846 1103 9 0.6703296703296715 1103 10-11 0.9945054945054963 1103 12-13 1.7472527472527446 1103 14-15 2.431318681318679 1103 16-17 3.689560439560438 1103 18-19 -2.228021978021978 1103 20-21 2.0357142857142847 1103 22-23 -3.793956043956044 1103 24-25 0.7417582417582409 1103 26-27 -3.384615384615387 1103 28-29 1.771978021978022 1103 30-31 3.5412087912087884 1103 32-33 3.4587912087912116 1103 34-35 -5.071428571428569 1103 36-37 -3.9065934065934087 1103 38-39 -3.664835164835168 1103 40-41 0.1318681318681314 1103 42-43 1.7747252747252773 1103 44-45 2.1401098901098905 1103 46-47 5.1098901098901095 1103 48-49 7.291208791208792 1103 50-51 0.5906593406593394 1103 52-53 1.939560439560438 1103 54-55 4.008241758241759 1103 56-57 0.541208791208792 1103 58-59 -5.719780219780219 1103 60-61 -6.23076923076923 1103 62-63 -5.912087912087912 1103 64-65 -5.291208791208792 1103 66-67 -5.98076923076923 1103 68-69 -4.423076923076923 1103 70-71 -4.543956043956044 1103 72-73 -6.28846153846154 1103 74-75 -4.225274725274726 1103 76-77 -2.167582417582416 1103 78-79 -3.1428571428571423 1103 80-81 -3.6813186813186825 1103 82-83 -1.3983516483516496 1103 84-85 -1.3434065934065949 1103 86-87 -1.552197802197803 1103 88-89 -1.799450549450551 1103 90-91 -0.3983516483516496 1103 92-93 -0.2417582417582409 1103 94-95 1.1153846153846168 1103 96-97 -0.7142857142857135 1103 98-99 3.3406593406593412 1103 100-101 5.8791208791208796 1104 1 0.708791208791208 1104 2 0.09890109890109855 1104 3 -0.73076923076923 1104 4 -1.137362637362635 1104 5 1.0494505494505475 1104 6 -8.340659340659343 1104 7 1.142857142857146 1104 8 0.9615384615384599 1104 9 -0.3296703296703285 1104 10-11 1.4945054945054963 1104 12-13 -0.2527472527472554 1104 14-15 1.181318681318679 1104 16-17 0.689560439560438 1104 18-19 2.021978021978022 1104 20-21 -14.714285714285715 1104 22-23 -1.0439560439560438 1104 24-25 -0.5082417582417591 1104 26-27 -0.1346153846153868 1104 28-29 3.521978021978022 1104 30-31 0.7912087912087884 1104 32-33 -7.791208791208788 1104 34-35 -14.32142857142857 1104 36-37 2.5934065934065913 1104 38-39 0.0851648351648322 1104 40-41 -3.6181318681318686 1104 42-43 -0.2252747252747227 1104 44-45 -14.10989010989011 1104 46-47 0.8598901098901095 1104 48-49 6.041208791208792 1104 50-51 4.3406593406593394 1104 52-53 4.439560439560438 1104 54-55 0.2582417582417591 1104 56-57 -11.208791208791208 1104 58-59 5.280219780219781 1104 60-61 10.76923076923077 1104 62-63 -5.162087912087912 1104 64-65 -2.041208791208792 1104 66-67 8.51923076923077 1104 68-69 5.576923076923077 1104 70-71 2.956043956043956 1104 72-73 10.46153846153846 1104 74-75 11.274725274725274 1104 76-77 8.582417582417584 1104 78-79 7.357142857142858 1104 80-81 -3.4313186813186825 1104 82-83 7.60164835164835 1104 84-85 10.156593406593405 1104 86-87 11.947802197802197 1104 88-89 10.700549450549449 1104 90-91 -9.64835164835165 1104 92-93 3.758241758241759 1104 94-95 -4.384615384615383 1104 96-97 14.285714285714286 1104 98-99 13.340659340659341 1104 100-101 -10.37087912087912 1105 1 0.708791208791208 1105 2 1.5989010989010985 1105 3 0.26923076923077005 1105 4 1.862637362637365 1105 5 1.0494505494505475 1105 6 1.659340659340657 1105 7 1.142857142857146 1105 8 1.96153846153846 1105 9 2.6703296703296715 1105 10-11 0.7445054945054963 1105 12-13 0.7472527472527446 1105 14-15 0.6813186813186789 1105 16-17 -1.060439560439562 1105 18-19 1.271978021978022 1105 20-21 3.2857142857142847 1105 22-23 1.9560439560439562 1105 24-25 0.2417582417582409 1105 26-27 4.365384615384613 1105 28-29 -7.978021978021978 1105 30-31 1.2912087912087884 1105 32-33 2.9587912087912116 1105 34-35 4.678571428571431 1105 36-37 0.5934065934065913 1105 38-39 4.085164835164832 1105 40-41 -1.8681318681318686 1105 42-43 0.024725274725277302 1105 44-45 3.8901098901098905 1105 46-47 2.3598901098901095 1105 48-49 1.791208791208792 1105 50-51 5.3406593406593394 1105 52-53 5.939560439560438 1105 54-55 3.758241758241759 1105 56-57 -3.458791208791208 1105 58-59 -7.719780219780219 1105 60-61 -15.73076923076923 1105 62-63 -22.162087912087912 1105 64-65 -21.541208791208792 1105 66-67 -21.48076923076923 1105 68-69 -20.923076923076923 1105 70-71 -21.043956043956044 1105 72-73 -20.03846153846154 1105 74-75 -19.225274725274726 1105 76-77 -18.417582417582416 1105 78-79 -19.642857142857142 1105 80-81 -19.431318681318682 1105 82-83 -17.89835164835165 1105 84-85 -17.843406593406595 1105 86-87 -18.052197802197803 1105 88-89 -17.29945054945055 1105 90-91 -16.64835164835165 1105 92-93 -16.74175824175824 1105 94-95 -15.384615384615383 1105 96-97 -13.714285714285714 1105 98-99 -12.659340659340659 1105 100-101 -10.37087912087912 1107 1 0.708791208791208 1107 2 0.09890109890109855 1107 3 0.26923076923077005 1107 4 0.8626373626373649 1107 5 1.0494505494505475 1107 6 1.659340659340657 1107 7 -0.3571428571428541 1107 8 1.96153846153846 1107 9 0.6703296703296715 1107 10-11 0.2445054945054963 1107 12-13 -0.002747252747255402 1107 14-15 -0.3186813186813211 1107 16-17 -0.310439560439562 1107 18-19 0.5219780219780219 1107 20-21 -1.7142857142857153 1107 22-23 1.9560439560439562 1107 24-25 3.991758241758241 1107 26-27 4.115384615384613 1107 28-29 2.271978021978022 1107 30-31 2.7912087912087884 1107 32-33 1.4587912087912116 1107 34-35 3.1785714285714306 1107 36-37 2.0934065934065913 1107 38-39 2.585164835164832 1107 40-41 2.6318681318681314 1107 42-43 3.2747252747252773 1107 44-45 4.8901098901098905 1107 46-47 5.8598901098901095 1107 48-49 -2.708791208791208 1107 50-51 -0.6593406593406606 1107 52-53 2.689560439560438 1107 54-55 -2.491758241758241 1107 56-57 2.041208791208792 1107 58-59 -6.719780219780219 1107 60-61 -5.98076923076923 1107 62-63 1.0879120879120876 1107 64-65 6.458791208791208 1107 66-67 2.51923076923077 1107 68-69 7.076923076923077 1107 70-71 0.4560439560439562 1107 72-73 2.71153846153846 1107 74-75 4.024725274725274 1107 76-77 7.082417582417584 1107 78-79 9.357142857142858 1107 80-81 -7.4313186813186825 1107 82-83 -7.39835164835165 1107 84-85 -10.593406593406595 1107 86-87 -3.052197802197803 1107 88-89 -4.299450549450551 1107 90-91 -4.64835164835165 1107 92-93 -5.241758241758241 1107 94-95 0.11538461538461675 1107 96-97 2.2857142857142865 1107 98-99 -4.159340659340659 1107 100-101 -10.37087912087912 1108 1 -0.29120879120879195 1108 2 0.09890109890109855 1108 3 0.26923076923077005 1108 4 0.8626373626373649 1108 5 1.0494505494505475 1108 6 0.659340659340657 1108 7 1.142857142857146 1108 8 0.9615384615384599 1108 9 -0.3296703296703285 1108 10-11 3.4945054945054963 1108 12-13 -3.2527472527472554 1108 14-15 -3.818681318681321 1108 16-17 -4.310439560439562 1108 18-19 -2.978021978021978 1108 20-21 -2.2142857142857153 1108 22-23 3.456043956043956 1108 24-25 -0.0082417582417591 1108 26-27 3.365384615384613 1108 28-29 0.5219780219780219 1108 30-31 -3.2087912087912116 1108 32-33 -0.2912087912087884 1108 34-35 -1.3214285714285694 1108 36-37 -2.9065934065934087 1108 38-39 1.5851648351648322 1108 40-41 -2.6181318681318686 1108 42-43 3.7747252747252773 1108 44-45 3.3901098901098905 1108 46-47 -0.1401098901098905 1108 48-49 1.041208791208792 1108 50-51 5.3406593406593394 1108 52-53 10.439560439560438 1108 54-55 0.2582417582417591 1108 56-57 8.791208791208792 1108 58-59 -4.219780219780219 1108 60-61 0.76923076923077 1108 62-63 2.3379120879120876 1108 64-65 -21.541208791208792 1108 66-67 -21.48076923076923 1108 68-69 -20.923076923076923 1108 70-71 -21.043956043956044 1108 72-73 -20.03846153846154 1108 74-75 -19.225274725274726 1108 76-77 -18.417582417582416 1108 78-79 -19.642857142857142 1108 80-81 -19.431318681318682 1108 82-83 -17.89835164835165 1108 84-85 -17.843406593406595 1108 86-87 -18.052197802197803 1108 88-89 -17.29945054945055 1108 90-91 -16.64835164835165 1108 92-93 -16.74175824175824 1108 94-95 -15.384615384615383 1108 96-97 -13.714285714285714 1108 98-99 -12.659340659340659 1108 100-101 -10.37087912087912 1109 1 -0.29120879120879195 1109 2 -0.9010989010989015 1109 3 0.26923076923077005 1109 4 0.8626373626373649 1109 5 -0.9505494505494525 1109 6 -4.340659340659343 1109 7 1.142857142857146 1109 8 0.9615384615384599 1109 9 -2.3296703296703285 1109 10-11 -1.0054945054945037 1109 12-13 -0.2527472527472554 1109 14-15 -0.8186813186813211 1109 16-17 -4.810439560439562 1109 18-19 0.5219780219780219 1109 20-21 -1.2142857142857153 1109 22-23 1.4560439560439562 1109 24-25 -3.008241758241759 1109 26-27 -3.134615384615387 1109 28-29 2.021978021978022 1109 30-31 -0.2087912087912116 1109 32-33 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12-13 1.7472527472527446 2314 14-15 2.681318681318679 2314 16-17 3.189560439560438 2314 18-19 5.521978021978022 2314 20-21 2.2857142857142847 2314 22-23 3.456043956043956 2314 24-25 2.991758241758241 2314 26-27 5.365384615384613 2314 28-29 6.021978021978022 2314 30-31 0.2912087912087884 2314 32-33 3.2087912087912116 2314 34-35 4.678571428571431 2314 36-37 3.5934065934065913 2314 38-39 5.085164835164832 2314 40-41 4.381868131868131 2314 42-43 5.774725274725277 2314 44-45 6.8901098901098905 2314 46-47 1.8598901098901095 2314 48-49 7.041208791208792 2314 50-51 5.3406593406593394 2314 52-53 5.439560439560438 2314 54-55 3.258241758241759 2314 56-57 12.291208791208792 2314 58-59 5.780219780219781 2314 60-61 9.26923076923077 2314 62-63 13.337912087912088 2314 64-65 14.958791208791208 2314 66-67 13.51923076923077 2314 68-69 14.076923076923077 2314 70-71 4.956043956043956 2314 72-73 -4.53846153846154 2314 74-75 -2.7252747252747263 2314 76-77 9.582417582417584 2314 78-79 9.857142857142858 2314 80-81 11.568681318681318 2314 82-83 10.60164835164835 2314 84-85 9.156593406593405 2314 86-87 8.947802197802197 2314 88-89 14.700549450549449 2314 90-91 16.35164835164835 2314 92-93 9.258241758241759 2314 94-95 17.115384615384617 2314 96-97 11.785714285714286 2314 98-99 16.340659340659343 2314 100-101 12.62912087912088 2315 1 0.708791208791208 2315 2 -20.9010989010989 2315 3 -2.73076923076923 2315 4 0.8626373626373649 2315 5 1.0494505494505475 2315 6 2.659340659340657 2315 7 -3.857142857142854 2315 8 0.9615384615384599 2315 9 1.6703296703296715 2315 10-11 3.4945054945054963 2315 12-13 3.7472527472527446 2315 14-15 2.181318681318679 2315 16-17 3.189560439560438 2315 18-19 1.521978021978022 2315 20-21 3.2857142857142847 2315 22-23 4.456043956043956 2315 24-25 3.991758241758241 2315 26-27 -4.134615384615387 2315 28-29 1.521978021978022 2315 30-31 1.2912087912087884 2315 32-33 5.708791208791212 2315 34-35 5.178571428571431 2315 36-37 5.093406593406591 2315 38-39 6.085164835164832 2315 40-41 -5.118131868131869 2315 42-43 -3.7252747252747227 2315 44-45 2.8901098901098905 2315 46-47 0.8598901098901095 2315 48-49 3.041208791208792 2315 50-51 7.3406593406593394 2315 52-53 4.939560439560438 2315 54-55 2.258241758241759 2315 56-57 7.291208791208792 2315 58-59 12.780219780219781 2315 60-61 10.26923076923077 2315 62-63 3.8379120879120876 2315 64-65 2.958791208791208 2315 66-67 14.51923076923077 2315 68-69 13.576923076923077 2315 70-71 16.956043956043956 2315 72-73 15.96153846153846 2315 74-75 13.774725274725274 2315 76-77 12.082417582417584 2315 78-79 8.857142857142858 2315 80-81 10.568681318681318 2315 82-83 12.10164835164835 2315 84-85 12.656593406593405 2315 86-87 12.447802197802197 2315 88-89 6.700549450549449 2315 90-91 -0.1483516483516496 2315 92-93 10.258241758241759 2315 94-95 -15.384615384615383 2315 96-97 -13.714285714285714 2315 98-99 -12.659340659340659 2315 100-101 -10.37087912087912 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 2.0 7 2.0 8 3.0 9 5.0 10 8.0 11 5.0 12 10.0 13 19.0 14 27.0 15 27.0 16 26.0 17 25.0 18 34.0 19 39.0 20 33.0 21 39.0 22 22.0 23 25.0 24 29.0 25 30.0 26 30.0 27 43.0 28 56.0 29 43.0 30 37.0 31 67.0 32 71.0 33 80.0 34 84.0 35 109.0 36 92.0 37 61.0 38 23.0 39 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.824503311258276 17.301324503311257 19.70198675496689 24.17218543046358 2 15.562913907284766 18.79139072847682 37.33443708609271 28.311258278145697 3 16.721854304635762 22.599337748344368 34.105960264900666 26.572847682119203 4 13.658940397350994 16.97019867549669 30.629139072847682 38.741721854304636 5 13.90728476821192 34.105960264900666 32.28476821192053 19.70198675496689 6 36.75496688741722 33.526490066225165 16.142384105960264 13.57615894039735 7 27.64900662251656 34.35430463576159 17.9635761589404 20.033112582781456 8 31.125827814569533 30.21523178807947 16.39072847682119 22.2682119205298 9 22.599337748344368 15.314569536423841 19.5364238410596 42.549668874172184 10-11 22.433774834437088 27.69039735099338 22.185430463576157 27.69039735099338 12-13 26.117549668874172 24.25496688741722 23.096026490066226 26.531456953642383 14-15 23.923841059602648 27.31788079470199 24.379139072847682 24.379139072847682 16-17 24.461920529801322 28.60099337748344 24.337748344370862 22.599337748344368 18-19 21.52317880794702 26.40728476821192 26.28311258278146 25.7864238410596 20-21 24.296357615894042 25.206953642384107 27.81456953642384 22.68211920529801 22-23 23.22019867549669 24.917218543046356 27.897350993377483 23.965231788079468 24-25 23.841059602649008 26.32450331125828 26.531456953642383 23.302980132450333 26-27 21.150662251655632 29.18046357615894 27.19370860927152 22.475165562913908 28-29 19.950331125827812 24.379139072847682 30.008278145695368 25.662251655629138 30-31 22.723509933774835 22.475165562913908 28.93211920529801 25.86920529801324 32-33 23.509933774834437 24.17218543046358 30.91887417218543 21.399006622516556 34-35 21.150662251655632 26.076158940397352 30.33940397350993 22.433774834437088 36-37 24.296357615894042 23.923841059602648 25.08278145695364 26.69701986754967 38-39 24.25496688741722 23.385761589403973 27.19370860927152 25.165562913907287 40-41 21.4817880794702 23.385761589403973 30.87748344370861 24.25496688741722 42-43 21.233443708609272 23.302980132450333 31.456953642384107 24.00662251655629 44-45 21.978476821192054 24.875827814569536 27.938741721854303 25.206953642384107 46-47 25.04139072847682 25.289735099337747 25.41390728476821 24.25496688741722 48-49 21.605960264900663 24.420529801324502 31.456953642384107 22.516556291390728 50-51 23.427152317880797 28.5182119205298 25.248344370860927 22.80629139072848 52-53 20.15728476821192 32.11920529801324 26.862582781456958 20.86092715231788 54-55 21.647350993377483 30.711920529801322 25.95198675496689 21.688741721854303 56-57 21.357615894039736 30.42218543046358 26.158940397350992 22.061258278145697 58-59 22.433774834437088 30.4635761589404 25.538079470198678 21.564569536423843 60-61 22.144039735099337 29.635761589403977 26.77980132450331 21.44039735099338 62-63 21.10927152317881 28.60099337748344 26.945364238410598 23.344370860927153 64-65 23.509933774834437 27.31788079470199 26.40728476821192 22.764900662251655 66-67 21.647350993377483 26.490066225165563 29.263245033112582 22.599337748344368 68-69 22.30960264900662 25.95198675496689 26.44867549668874 25.289735099337747 70-71 23.178807947019866 26.531456953642383 25.86920529801324 24.420529801324502 72-73 22.80629139072848 26.986754966887418 26.572847682119203 23.6341059602649 74-75 21.399006622516556 28.14569536423841 26.03476821192053 24.420529801324502 76-77 23.271221532091097 26.666666666666668 27.28778467908903 22.77432712215321 78-79 22.764900662251655 27.40066225165563 25.86920529801324 23.965231788079468 80-81 22.48447204968944 28.033126293995856 25.341614906832298 24.1407867494824 82-83 21.730132450331126 27.77317880794702 27.81456953642384 22.68211920529801 84-85 23.354037267080745 27.660455486542446 25.175983436853 23.809523809523807 86-87 23.178807947019866 27.77317880794702 25.99337748344371 23.054635761589402 88-89 23.716887417218544 26.77980132450331 25.86920529801324 23.6341059602649 90-91 22.89855072463768 27.246376811594203 25.631469979296067 24.22360248447205 92-93 23.67549668874172 27.483443708609272 26.076158940397352 22.764900662251655 94-95 22.061258278145697 27.27649006622517 25.579470198675498 25.08278145695364 96-97 22.350993377483444 26.200331125827812 26.614238410596023 24.834437086092713 98-99 22.144039735099337 25.08278145695364 26.076158940397352 26.69701986754967 100-101 22.185430463576157 26.32450331125828 25.74503311258278 25.74503311258278 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1.0 1 0.5 2 0.0 3 0.5 4 0.5 5 0.0 6 0.0 7 0.5 8 1.0 9 1.0 10 0.5 11 1.5 12 2.5 13 2.5 14 2.0 15 1.5 16 1.5 17 1.0 18 0.5 19 0.5 20 0.5 21 0.5 22 2.0 23 5.0 24 4.5 25 4.5 26 5.0 27 6.5 28 7.0 29 7.5 30 9.0 31 11.5 32 16.5 33 16.5 34 22.0 35 22.0 36 19.0 37 21.0 38 17.0 39 17.0 40 24.0 41 28.5 42 35.5 43 39.0 44 39.5 45 43.5 46 47.0 47 52.5 48 55.0 49 55.0 50 56.0 51 58.0 52 58.0 53 54.0 54 50.0 55 48.0 56 39.5 57 33.0 58 31.5 59 28.5 60 22.5 61 14.0 62 10.5 63 9.5 64 8.5 65 10.0 66 9.0 67 4.0 68 3.5 69 3.0 70 1.0 71 1.0 72 0.5 73 0.5 74 0.5 75 0.0 76 0.5 77 0.5 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.041390728476821195 78-79 0.0 80-81 0.041390728476821195 82-83 0.0 84-85 0.041390728476821195 86-87 0.0 88-89 0.0 90-91 0.041390728476821195 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1208.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 92.54966887417218 #Duplication Level Percentage of deduplicated Percentage of total 1 96.51162790697676 89.32119205298014 2 1.9677996422182469 3.642384105960265 3 0.5366726296958855 1.490066225165563 4 0.4472271914132379 1.6556291390728477 5 0.17889087656529518 0.8278145695364238 6 0.0 0.0 7 0.08944543828264759 0.5794701986754967 8 0.08944543828264759 0.6622516556291391 9 0.0 0.0 >10 0.17889087656529518 1.8211920529801324 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG 12 0.9933774834437087 RNA PCR Primer, Index 33 (96% over 29bp) TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG 10 0.8278145695364238 RNA PCR Primer, Index 31 (96% over 29bp) TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG 8 0.6622516556291391 RNA PCR Primer, Index 39 (96% over 30bp) TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG 7 0.5794701986754967 RNA PCR Primer, Index 17 (96% over 28bp) CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGA 5 0.4139072847682119 RNA PCR Primer, Index 33 (96% over 29bp) TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG 5 0.4139072847682119 RNA PCR Primer, Index 23 (96% over 30bp) TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG 4 0.33112582781456956 RNA PCR Primer, Index 46 (96% over 28bp) TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG 4 0.33112582781456956 RNA PCR Primer, Index 26 (100% over 29bp) GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4 0.33112582781456956 No Hit TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC 4 0.33112582781456956 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4 0.33112582781456956 No Hit CAGCTGTTCTCATCATGATCTTTATAATTTGAGAGGCCTTTGCTTCAAAA 3 0.24834437086092717 No Hit TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG 3 0.24834437086092717 TruSeq Adapter, Index 3 (96% over 28bp) CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT 3 0.24834437086092717 No Hit GTGCTCGCCCACGTCGCTGTCGTAGCGCACGTACTCCTCCCGGTTGTAGA 3 0.24834437086092717 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3 0.24834437086092717 No Hit GTCCCTCACCCTCCCAAAAGCCACCCCCACTCCTAAGAGGAGGATGGTCG 3 0.24834437086092717 No Hit GTTCTACAAATGTGGCTGAGGACTTTGTACATTGTTTTGTTTTTTTTTTT 2 0.16556291390728478 No Hit GAACTGCTACGTAACACAGTTCCACCCGCCTCACATTGAAATCCAAATGC 2 0.16556291390728478 No Hit CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG 2 0.16556291390728478 No Hit ATGCTACACATTGGGAGCACATCCATGTCGGGTTGGAAAAGACAGTCCTC 2 0.16556291390728478 No Hit TCTCCGAGCCCACGAGACCAGAGAGGATCTCGGATGCCGTCTTCTGCTTG 2 0.16556291390728478 Illumina Paired End PCR Primer 2 (96% over 25bp) AAGTAGAGATGTCAGATATGTCCTTCAGCAAGGACTGGTCTTTCTATATC 2 0.16556291390728478 No Hit CTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTGA 2 0.16556291390728478 RNA PCR Primer, Index 23 (96% over 30bp) CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGA 2 0.16556291390728478 RNA PCR Primer, Index 10 (96% over 28bp) GGTATCAACGCAGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT 2 0.16556291390728478 RNA PCR Primer, Index 31 (95% over 22bp) CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGA 2 0.16556291390728478 RNA PCR Primer, Index 39 (96% over 30bp) TCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAA 2 0.16556291390728478 TruSeq Adapter, Index 3 (96% over 28bp) GGATTTAAAAACTGGAACGGTGAAGGCGACAGCAGTTGGTTGGAGCAAAC 2 0.16556291390728478 No Hit CTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTTATA 2 0.16556291390728478 No Hit GAGCATATGGGGCGACGCTCGTCGAAATGCTGCTGTATTTATGAGAAGCC 2 0.16556291390728478 No Hit TCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAA 2 0.16556291390728478 Illumina PCR Primer Index 11 (96% over 29bp) CTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGA 2 0.16556291390728478 RNA PCR Primer, Index 31 (96% over 29bp) CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2 0.16556291390728478 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2 0.16556291390728478 No Hit CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGA 2 0.16556291390728478 RNA PCR Primer, Index 1 (96% over 28bp) GTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTAAATTATGTACTG 2 0.16556291390728478 No Hit GTCGTAGCGCACGTACTCCTCCCGGTTGTAGATGTATCTGGTCACATCTC 2 0.16556291390728478 No Hit CGCCACCCACCGGAGAATGGGAAGCCGAACATACTGAACTGCTACGTAAC 2 0.16556291390728478 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.08278145695364239 0.0 42-43 0.0 0.0 0.0 0.16556291390728478 0.0 44-45 0.0 0.0 0.0 0.2897350993377483 0.0 46-47 0.0 0.0 0.0 0.37251655629139074 0.0 48-49 0.0 0.0 0.0 0.4139072847682119 0.0 50-51 0.0 0.0 0.0 0.6208609271523179 0.0 52-53 0.0 0.0 0.0 0.6622516556291391 0.0 54-55 0.0 0.0 0.0 0.869205298013245 0.0 56-57 0.0 0.0 0.0 0.9933774834437086 0.0 58-59 0.0 0.0 0.0 1.076158940397351 0.0 60-61 0.0 0.0 0.0 1.1175496688741722 0.0 62-63 0.0 0.0 0.0 1.3245033112582782 0.0 64-65 0.0 0.0 0.0 1.4486754966887418 0.0 66-67 0.0 0.0 0.0 1.5728476821192052 0.0 68-69 0.0 0.0 0.0 1.697019867549669 0.0 70-71 0.0 0.0 0.0 1.7384105960264902 0.0 72-73 0.0 0.0 0.0 1.9867549668874172 0.0 74-75 0.0 0.0 0.0 2.2350993377483444 0.0 76-77 0.0 0.0 0.0 2.2764900662251657 0.0 78-79 0.0 0.0 0.0 2.6490066225165565 0.0 80-81 0.0 0.0 0.0 2.814569536423841 0.0 82-83 0.0 0.0 0.0 3.104304635761589 0.0 84-85 0.0 0.0 0.0 3.435430463576159 0.0 86-87 0.0 0.0 0.0 3.725165562913907 0.0 88-89 0.0 0.0 0.0 3.890728476821192 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE