FastQCFastQC Report
Thu 26 May 2016
SRR938490_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938490_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1312
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG151.1432926829268293RNA PCR Primer, Index 39 (96% over 30bp)
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG141.0670731707317074RNA PCR Primer, Index 33 (96% over 29bp)
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG130.9908536585365854RNA PCR Primer, Index 31 (96% over 29bp)
GGATTTAAAAACTGGAACGGTGAAGGCGACAGCAGTTGGTTGGAGCAAAC80.6097560975609756No Hit
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGA80.6097560975609756RNA PCR Primer, Index 33 (96% over 29bp)
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG80.6097560975609756TruSeq Adapter, Index 10 (96% over 28bp)
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG70.5335365853658537RNA PCR Primer, Index 27 (96% over 30bp)
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG60.45731707317073167TruSeq Adapter, Index 3 (96% over 28bp)
ACTCAGACTACCCAGATGCTTACACCACATGAAACACTGTCTCTTCTATA50.38109756097560976No Hit
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG50.38109756097560976RNA PCR Primer, Index 23 (96% over 30bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.3048780487804878No Hit
TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC40.3048780487804878No Hit
CTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGA40.3048780487804878RNA PCR Primer, Index 31 (96% over 29bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.22865853658536583No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG30.22865853658536583RNA PCR Primer, Index 17 (96% over 28bp)
ATACTGAACTGCTACGTAACACAGTTCCACCCGCCTCACATTGAAATCCA30.22865853658536583No Hit
CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT30.22865853658536583No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA30.22865853658536583No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.22865853658536583No Hit
GTTGTATACAAACCAAACTTCATGATTAAATGCAAGCACAGTTTCTGAAT20.1524390243902439No Hit
TCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAA20.1524390243902439TruSeq Adapter, Index 1 (96% over 28bp)
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGA20.1524390243902439RNA PCR Primer, Index 46 (96% over 28bp)
GAGCAAACATCCCCCAAAGTTCTACAAATGTGGCTGAGGACTTTGTACAT20.1524390243902439No Hit
CTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCTGGCTGCCTCAACA20.1524390243902439No Hit
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGA20.1524390243902439RNA PCR Primer, Index 39 (96% over 30bp)
GTGTTTGCTATCATAGCAGGATTTGTTCACTGATTCCCATTATTTTCAGG20.1524390243902439No Hit
TCAGTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAG20.1524390243902439No Hit
TCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAA20.1524390243902439RNA PCR Primer, Index 27 (100% over 28bp)
CTGCAGCTGCCCTCACCATCATCCTCACTGCAGCCGCCCTCTGCACCCCC20.1524390243902439No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG20.1524390243902439RNA PCR Primer, Index 26 (100% over 29bp)
ATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTTATAAT20.1524390243902439No Hit
GTGCTCGCCCACGTCGCTGTCGTAGCGCACGTACTCCTCCCGGTTGTAGA20.1524390243902439No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA20.1524390243902439No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.1524390243902439No Hit
TCTGCACACCGTGTCCAGCTCGGCCCGCGTTCGCTCCAGGATCTCCGGCT20.1524390243902439No Hit
CTCTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCT20.1524390243902439No Hit
GTATCCATGAAATAAGTGGTTACAGGAAGTCCCTCACCCTCCCAAAAGCC20.1524390243902439No Hit
CTAACAGACCGCAACCTAAACACAACTTTCTTTGATCCCGCTGGAGGAGG20.1524390243902439No Hit
TTTCAGGAATACCACGACGCTACTCAGACTACCCAGATGCTTACACCACA20.1524390243902439No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC20.1524390243902439No Hit
ACCCAGATGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTT20.1524390243902439No Hit
GTTCCACCCGCCTCACATTGAAATCCAAATGCTGAAGAACGGGAAAAAAA20.1524390243902439No Hit
ATGAAACACTGTCTCTTCTATAGGATCATTTATTTCACTAACAGCTGTTC20.1524390243902439No Hit
GTATACTGAATACATTGTTCCACATGGAGGTGGGGAGACAGAGACCGTCA20.1524390243902439No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAAC250.00396898157.01
AACGCAG300.00817163147.55
TCAACGC300.00817163147.53
ATCAACG300.00817163147.52
CAACGCA300.00817163147.54
Produced by FastQC (version 0.11.5)