##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938489_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1238 Sequences flagged as poor quality 0 Sequence length 101 %GC 49 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.836025848142164 26.0 10.0 31.0 2.0 33.0 2 20.71647819063005 26.0 10.0 31.0 2.0 33.0 3 20.6421647819063 26.0 10.0 31.0 2.0 33.0 4 23.60420032310178 32.0 10.0 35.0 2.0 37.0 5 22.894991922455574 30.0 10.0 35.0 2.0 37.0 6 23.103392568659128 31.0 8.0 35.0 2.0 37.0 7 22.88529886914378 30.0 2.0 35.0 2.0 37.0 8 22.14297253634895 30.0 2.0 35.0 2.0 37.0 9 22.955573505654282 30.0 2.0 37.0 2.0 39.0 10-11 22.309773828756057 29.0 2.0 37.0 2.0 39.0 12-13 21.738691437802906 27.0 2.0 37.0 2.0 39.0 14-15 21.584410339256866 27.0 2.0 37.5 2.0 40.0 16-17 21.151857835218095 26.0 2.0 37.0 2.0 40.0 18-19 21.17366720516963 27.0 2.0 37.0 2.0 40.0 20-21 20.576736672051695 25.0 2.0 37.0 2.0 40.0 22-23 20.796445880452342 26.5 2.0 37.0 2.0 40.0 24-25 20.303715670436187 25.0 2.0 37.0 2.0 40.0 26-27 20.107431340872374 24.0 2.0 37.0 2.0 40.0 28-29 19.89458804523425 24.0 2.0 37.0 2.0 40.0 30-31 19.34491114701131 21.5 2.0 36.5 2.0 40.0 32-33 18.714458804523424 17.0 2.0 36.0 2.0 39.5 34-35 18.30573505654281 16.0 2.0 35.0 2.0 39.5 36-37 17.941841680129244 14.0 2.0 35.0 2.0 40.0 38-39 17.729806138933768 11.5 2.0 35.0 2.0 39.0 40-41 17.345315024232633 9.0 2.0 35.0 2.0 39.0 42-43 17.149434571890147 8.0 2.0 35.0 2.0 39.0 44-45 17.02665589660743 5.0 2.0 35.0 2.0 39.0 46-47 16.3218901453958 2.0 2.0 34.0 2.0 38.5 48-49 15.889337641357027 2.0 2.0 33.0 2.0 38.5 50-51 15.50767366720517 2.0 2.0 32.5 2.0 38.5 52-53 15.386510500807756 2.0 2.0 33.0 2.0 38.0 54-55 15.228190630048466 2.0 2.0 33.0 2.0 38.0 56-57 14.983441033925686 2.0 2.0 33.0 2.0 38.0 58-59 14.691437802907917 2.0 2.0 33.0 2.0 38.0 60-61 14.169628432956381 2.0 2.0 32.0 2.0 38.0 62-63 13.641760904684975 2.0 2.0 31.0 2.0 37.5 64-65 13.397819063004846 2.0 2.0 31.0 2.0 37.0 66-67 12.756865912762521 2.0 2.0 30.5 2.0 36.0 68-69 12.319063004846527 2.0 2.0 28.5 2.0 36.0 70-71 11.890953150242327 2.0 2.0 28.0 2.0 35.5 72-73 11.745153473344104 2.0 2.0 28.5 2.0 35.0 74-75 11.458400646203554 2.0 2.0 26.5 2.0 35.0 76-77 10.968901453957997 2.0 2.0 26.0 2.0 34.0 78-79 10.615912762520193 2.0 2.0 25.0 2.0 34.0 80-81 10.432956381260098 2.0 2.0 24.5 2.0 34.0 82-83 9.87843295638126 2.0 2.0 21.0 2.0 34.0 84-85 9.484248788368337 2.0 2.0 18.0 2.0 34.0 86-87 9.172455573505655 2.0 2.0 12.0 2.0 33.5 88-89 8.789579967689821 2.0 2.0 3.5 2.0 32.5 90-91 8.391760904684975 2.0 2.0 2.0 2.0 32.0 92-93 8.098142164781907 2.0 2.0 2.0 2.0 31.5 94-95 7.734248788368336 2.0 2.0 2.0 2.0 31.5 96-97 7.209612277867528 2.0 2.0 2.0 2.0 31.0 98-99 6.653473344103393 2.0 2.0 2.0 2.0 30.0 100-101 5.919628432956381 2.0 2.0 2.0 2.0 28.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1102 1 7.882783882783883 1102 2 10.265567765567763 1102 3 9.227106227106226 1102 4 -0.15567765567765335 1102 5 9.386446886446883 1102 6 10.247252747252748 1102 7 1.1080586080586095 1102 8 4.137362637362639 1102 9 11.326007326007325 1102 10-11 6.523809523809522 1102 12-13 13.740842490842489 1102 14-15 -0.06868131868131755 1102 16-17 -1.289377289377292 1102 18-19 -0.47435897435897445 1102 20-21 -0.0787545787545767 1102 22-23 -3.965201465201467 1102 24-25 -1.989010989010989 1102 26-27 -4.6465201465201496 1102 28-29 -1.9642857142857153 1102 30-31 0.5457875457875438 1102 32-33 1.6575091575091605 1102 34-35 1.3791208791208796 1102 36-37 0.5769230769230766 1102 38-39 0.3910256410256423 1102 40-41 -0.03388278388278465 1102 42-43 0.9313186813186825 1102 44-45 1.6089743589743577 1102 46-47 2.9926739926739927 1102 48-49 1.6062271062271058 1102 50-51 -2.0228937728937737 1102 52-53 2.3058608058608083 1102 54-55 2.726190476190478 1102 56-57 -4.032967032967033 1102 58-59 -2.0796703296703303 1102 60-61 -3.5265567765567756 1102 62-63 2.5494505494505493 1102 64-65 6.386446886446885 1102 66-67 5.51923076923077 1102 68-69 -6.423992673992673 1102 70-71 -4.942307692307692 1102 72-73 -0.5860805860805858 1102 74-75 4.636446886446887 1102 76-77 6.32967032967033 1102 78-79 -2.833333333333332 1102 80-81 5.55952380952381 1102 82-83 0.7445054945054945 1102 84-85 1.6538461538461533 1102 86-87 3.0283882783882774 1102 88-89 0.8489010989010985 1102 90-91 5.712454212454212 1102 92-93 4.8489010989010985 1102 94-95 10.076007326007327 1102 96-97 9.012820512820515 1102 98-99 -3.3717948717948723 1102 100-101 -2.9761904761904763 1103 1 4.882783882783883 1103 2 5.265567765567763 1103 3 10.227106227106226 1103 4 -0.15567765567765335 1103 5 6.386446886446883 1103 6 7.747252747252748 1103 7 1.6080586080586095 1103 8 4.137362637362639 1103 9 2.826007326007325 1103 10-11 1.273809523809522 1103 12-13 4.990842490842489 1103 14-15 3.6813186813186825 1103 16-17 9.710622710622708 1103 18-19 9.275641025641026 1103 20-21 12.421245421245423 1103 22-23 12.284798534798533 1103 24-25 11.760989010989011 1103 26-27 13.60347985347985 1103 28-29 13.035714285714285 1103 30-31 3.295787545787544 1103 32-33 0.9075091575091605 1103 34-35 -3.8708791208791204 1103 36-37 -0.17307692307692335 1103 38-39 0.1410256410256423 1103 40-41 -0.5338827838827847 1103 42-43 2.6813186813186825 1103 44-45 2.1089743589743577 1103 46-47 0.2426739926739927 1103 48-49 2.606227106227106 1103 50-51 3.9771062271062263 1103 52-53 4.305860805860808 1103 54-55 2.976190476190478 1103 56-57 3.967032967032967 1103 58-59 5.92032967032967 1103 60-61 -0.026556776556775574 1103 62-63 3.0494505494505493 1103 64-65 7.386446886446885 1103 66-67 8.26923076923077 1103 68-69 9.076007326007327 1103 70-71 8.307692307692308 1103 72-73 5.913919413919414 1103 74-75 7.386446886446887 1103 76-77 6.57967032967033 1103 78-79 -8.333333333333332 1103 80-81 -8.44047619047619 1103 82-83 -7.7554945054945055 1103 84-85 -7.096153846153847 1103 86-87 -6.471611721611723 1103 88-89 -6.4010989010989015 1103 90-91 -6.287545787545788 1103 92-93 -5.1510989010989015 1103 94-95 -5.173992673992673 1103 96-97 -4.237179487179486 1103 98-99 -3.3717948717948723 1103 100-101 -2.9761904761904763 1104 1 -17.617216117216117 1104 2 -17.734432234432237 1104 3 -17.272893772893774 1104 4 -20.655677655677653 1104 5 -18.613553113553117 1104 6 -19.252747252747252 1104 7 -19.39194139194139 1104 8 -19.86263736263736 1104 9 -20.173992673992675 1104 10-11 -19.726190476190478 1104 12-13 -19.25915750915751 1104 14-15 -18.318681318681318 1104 16-17 -19.039377289377292 1104 18-19 -18.724358974358974 1104 20-21 -18.078754578754577 1104 22-23 -18.965201465201467 1104 24-25 -18.48901098901099 1104 26-27 -18.39652014652015 1104 28-29 -18.464285714285715 1104 30-31 -17.954212454212456 1104 32-33 -16.84249084249084 1104 34-35 -17.12087912087912 1104 36-37 -17.423076923076923 1104 38-39 -17.108974358974358 1104 40-41 -17.283882783882785 1104 42-43 -16.318681318681318 1104 44-45 -16.141025641025642 1104 46-47 -14.507326007326007 1104 48-49 -14.643772893772894 1104 50-51 -14.272893772893774 1104 52-53 -13.444139194139192 1104 54-55 -13.023809523809522 1104 56-57 -12.282967032967033 1104 58-59 -12.82967032967033 1104 60-61 -11.776556776556776 1104 62-63 -11.95054945054945 1104 64-65 -11.613553113553115 1104 66-67 -10.48076923076923 1104 68-69 -9.423992673992673 1104 70-71 -9.192307692307692 1104 72-73 -9.336080586080586 1104 74-75 -8.863553113553113 1104 76-77 -8.92032967032967 1104 78-79 -8.333333333333332 1104 80-81 -8.44047619047619 1104 82-83 -7.7554945054945055 1104 84-85 -7.096153846153847 1104 86-87 -6.471611721611723 1104 88-89 -6.4010989010989015 1104 90-91 -6.287545787545788 1104 92-93 -5.1510989010989015 1104 94-95 -5.173992673992673 1104 96-97 -4.237179487179486 1104 98-99 -3.3717948717948723 1104 100-101 -2.9761904761904763 1105 1 -9.617216117216117 1105 2 -9.734432234432237 1105 3 -9.272893772893774 1105 4 -12.655677655677653 1105 5 -1.6135531135531167 1105 6 9.747252747252748 1105 7 13.60805860805861 1105 8 4.137362637362639 1105 9 11.826007326007325 1105 10-11 11.273809523809522 1105 12-13 4.240842490842489 1105 14-15 10.181318681318682 1105 16-17 13.960622710622708 1105 18-19 9.275641025641026 1105 20-21 4.921245421245423 1105 22-23 -3.965201465201467 1105 24-25 0.01098901098901095 1105 26-27 0.6034798534798504 1105 28-29 -8.964285714285715 1105 30-31 -4.454212454212456 1105 32-33 -6.8424908424908395 1105 34-35 -2.6208791208791204 1105 36-37 1.5769230769230766 1105 38-39 7.391025641025642 1105 40-41 15.716117216117215 1105 42-43 9.681318681318682 1105 44-45 -7.641025641025642 1105 46-47 12.992673992673993 1105 48-49 11.856227106227106 1105 50-51 2.2271062271062263 1105 52-53 12.555860805860808 1105 54-55 6.976190476190478 1105 56-57 9.217032967032967 1105 58-59 12.17032967032967 1105 60-61 10.723443223443224 1105 62-63 14.04945054945055 1105 64-65 -11.613553113553115 1105 66-67 -10.48076923076923 1105 68-69 -9.423992673992673 1105 70-71 -9.192307692307692 1105 72-73 -9.336080586080586 1105 74-75 -8.863553113553113 1105 76-77 -8.92032967032967 1105 78-79 -8.333333333333332 1105 80-81 -8.44047619047619 1105 82-83 -7.7554945054945055 1105 84-85 -7.096153846153847 1105 86-87 -6.471611721611723 1105 88-89 -6.4010989010989015 1105 90-91 -6.287545787545788 1105 92-93 -5.1510989010989015 1105 94-95 -5.173992673992673 1105 96-97 -4.237179487179486 1105 98-99 -3.3717948717948723 1105 100-101 -2.9761904761904763 1106 1 -17.617216117216117 1106 2 -17.734432234432237 1106 3 -17.272893772893774 1106 4 -20.655677655677653 1106 5 -18.613553113553117 1106 6 -19.252747252747252 1106 7 -19.39194139194139 1106 8 -19.86263736263736 1106 9 -20.173992673992675 1106 10-11 -19.726190476190478 1106 12-13 -19.25915750915751 1106 14-15 -18.318681318681318 1106 16-17 -19.039377289377292 1106 18-19 -18.724358974358974 1106 20-21 -18.078754578754577 1106 22-23 -18.965201465201467 1106 24-25 -18.48901098901099 1106 26-27 -18.39652014652015 1106 28-29 -18.464285714285715 1106 30-31 -17.954212454212456 1106 32-33 -16.84249084249084 1106 34-35 -17.12087912087912 1106 36-37 -17.423076923076923 1106 38-39 -17.108974358974358 1106 40-41 -17.283882783882785 1106 42-43 -16.318681318681318 1106 44-45 -16.141025641025642 1106 46-47 -14.507326007326007 1106 48-49 -14.643772893772894 1106 50-51 -14.272893772893774 1106 52-53 -13.444139194139192 1106 54-55 -13.023809523809522 1106 56-57 -12.282967032967033 1106 58-59 -12.82967032967033 1106 60-61 -11.776556776556776 1106 62-63 -11.95054945054945 1106 64-65 -11.613553113553115 1106 66-67 -10.48076923076923 1106 68-69 -9.423992673992673 1106 70-71 -9.192307692307692 1106 72-73 -9.336080586080586 1106 74-75 -8.863553113553113 1106 76-77 -8.92032967032967 1106 78-79 -8.333333333333332 1106 80-81 -8.44047619047619 1106 82-83 -7.7554945054945055 1106 84-85 -7.096153846153847 1106 86-87 -6.471611721611723 1106 88-89 -6.4010989010989015 1106 90-91 -6.287545787545788 1106 92-93 -5.1510989010989015 1106 94-95 -5.173992673992673 1106 96-97 -4.237179487179486 1106 98-99 -3.3717948717948723 1106 100-101 -2.9761904761904763 1107 1 14.382783882783883 1107 2 11.265567765567763 1107 3 14.727106227106226 1107 4 14.344322344322347 1107 5 16.386446886446883 1107 6 15.747252747252748 1107 7 15.60805860805861 1107 8 15.137362637362639 1107 9 15.826007326007325 1107 10-11 17.273809523809522 1107 12-13 14.740842490842489 1107 14-15 15.181318681318682 1107 16-17 16.460622710622708 1107 18-19 17.775641025641026 1107 20-21 13.421245421245423 1107 22-23 17.034798534798533 1107 24-25 17.51098901098901 1107 26-27 18.10347985347985 1107 28-29 19.035714285714285 1107 30-31 17.045787545787544 1107 32-33 18.65750915750916 1107 34-35 19.87912087912088 1107 36-37 16.576923076923077 1107 38-39 16.891025641025642 1107 40-41 18.716117216117215 1107 42-43 17.681318681318682 1107 44-45 20.858974358974358 1107 46-47 23.492673992673993 1107 48-49 18.856227106227106 1107 50-51 21.727106227106226 1107 52-53 21.55586080586081 1107 54-55 23.976190476190478 1107 56-57 24.217032967032967 1107 58-59 24.17032967032967 1107 60-61 23.223443223443226 1107 62-63 24.549450549450547 1107 64-65 24.886446886446883 1107 66-67 27.51923076923077 1107 68-69 27.57600732600733 1107 70-71 27.807692307692307 1107 72-73 27.163919413919416 1107 74-75 24.636446886446887 1107 76-77 24.07967032967033 1107 78-79 26.166666666666668 1107 80-81 26.05952380952381 1107 82-83 26.244505494505496 1107 84-85 24.903846153846153 1107 86-87 25.528388278388277 1107 88-89 24.5989010989011 1107 90-91 26.21245421245421 1107 92-93 27.3489010989011 1107 94-95 24.32600732600733 1107 96-97 26.762820512820515 1107 98-99 27.628205128205128 1107 100-101 11.023809523809524 1108 1 -17.617216117216117 1108 2 -17.734432234432237 1108 3 -17.272893772893774 1108 4 -20.655677655677653 1108 5 -18.613553113553117 1108 6 -19.252747252747252 1108 7 -19.39194139194139 1108 8 -19.86263736263736 1108 9 -20.173992673992675 1108 10-11 -19.726190476190478 1108 12-13 -19.25915750915751 1108 14-15 -18.318681318681318 1108 16-17 -19.039377289377292 1108 18-19 -18.724358974358974 1108 20-21 -18.078754578754577 1108 22-23 -18.965201465201467 1108 24-25 -18.48901098901099 1108 26-27 -18.39652014652015 1108 28-29 -18.464285714285715 1108 30-31 -17.954212454212456 1108 32-33 -16.84249084249084 1108 34-35 -17.12087912087912 1108 36-37 -17.423076923076923 1108 38-39 -17.108974358974358 1108 40-41 -17.283882783882785 1108 42-43 -16.318681318681318 1108 44-45 -16.141025641025642 1108 46-47 -14.507326007326007 1108 48-49 -14.643772893772894 1108 50-51 -14.272893772893774 1108 52-53 -13.444139194139192 1108 54-55 -13.023809523809522 1108 56-57 -12.282967032967033 1108 58-59 -12.82967032967033 1108 60-61 -11.776556776556776 1108 62-63 -11.95054945054945 1108 64-65 -11.613553113553115 1108 66-67 -10.48076923076923 1108 68-69 -9.423992673992673 1108 70-71 -9.192307692307692 1108 72-73 -9.336080586080586 1108 74-75 -8.863553113553113 1108 76-77 -8.92032967032967 1108 78-79 -8.333333333333332 1108 80-81 -8.44047619047619 1108 82-83 -7.7554945054945055 1108 84-85 -7.096153846153847 1108 86-87 -6.471611721611723 1108 88-89 -6.4010989010989015 1108 90-91 -6.287545787545788 1108 92-93 -5.1510989010989015 1108 94-95 -5.173992673992673 1108 96-97 -4.237179487179486 1108 98-99 -3.3717948717948723 1108 100-101 -2.9761904761904763 1109 1 14.382783882783883 1109 2 14.265567765567763 1109 3 14.727106227106226 1109 4 14.344322344322347 1109 5 16.386446886446883 1109 6 15.747252747252748 1109 7 15.60805860805861 1109 8 15.137362637362639 1109 9 16.826007326007325 1109 10-11 17.273809523809522 1109 12-13 17.74084249084249 1109 14-15 18.181318681318682 1109 16-17 19.460622710622708 1109 18-19 19.775641025641026 1109 20-21 20.921245421245423 1109 22-23 20.034798534798533 1109 24-25 20.51098901098901 1109 26-27 20.60347985347985 1109 28-29 20.535714285714285 1109 30-31 20.545787545787544 1109 32-33 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-18.318681318681318 2316 16-17 -19.039377289377292 2316 18-19 -18.724358974358974 2316 20-21 -18.078754578754577 2316 22-23 -18.965201465201467 2316 24-25 -18.48901098901099 2316 26-27 -18.39652014652015 2316 28-29 -18.464285714285715 2316 30-31 -17.954212454212456 2316 32-33 -16.84249084249084 2316 34-35 -17.12087912087912 2316 36-37 -17.423076923076923 2316 38-39 -17.108974358974358 2316 40-41 -17.283882783882785 2316 42-43 -16.318681318681318 2316 44-45 -16.141025641025642 2316 46-47 -14.507326007326007 2316 48-49 -14.643772893772894 2316 50-51 -14.272893772893774 2316 52-53 -13.444139194139192 2316 54-55 -13.023809523809522 2316 56-57 -12.282967032967033 2316 58-59 -12.82967032967033 2316 60-61 -11.776556776556776 2316 62-63 -11.95054945054945 2316 64-65 -11.613553113553115 2316 66-67 -10.48076923076923 2316 68-69 -9.423992673992673 2316 70-71 -9.192307692307692 2316 72-73 -9.336080586080586 2316 74-75 -8.863553113553113 2316 76-77 -8.92032967032967 2316 78-79 -8.333333333333332 2316 80-81 -8.44047619047619 2316 82-83 -7.7554945054945055 2316 84-85 -7.096153846153847 2316 86-87 -6.471611721611723 2316 88-89 -6.4010989010989015 2316 90-91 -6.287545787545788 2316 92-93 -5.1510989010989015 2316 94-95 -5.173992673992673 2316 96-97 -4.237179487179486 2316 98-99 -3.3717948717948723 2316 100-101 -2.9761904761904763 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 296.0 3 72.0 4 41.0 5 31.0 6 33.0 7 32.0 8 27.0 9 34.0 10 31.0 11 16.0 12 25.0 13 30.0 14 21.0 15 17.0 16 22.0 17 21.0 18 19.0 19 17.0 20 26.0 21 20.0 22 16.0 23 21.0 24 22.0 25 22.0 26 18.0 27 21.0 28 19.0 29 24.0 30 18.0 31 33.0 32 26.0 33 36.0 34 28.0 35 33.0 36 35.0 37 28.0 38 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.23886639676113 20.728744939271255 12.955465587044534 23.076923076923077 2 18.997574777687955 21.827000808407437 37.10590137429264 22.069523039611965 3 23.039611964430073 23.60549717057397 28.37510105092967 24.97978981406629 4 16.006467259498788 15.278900565885206 33.225545675020214 35.48908649959579 5 18.755052546483427 35.40824575586095 31.608730800323364 14.227970897332257 6 33.38722716248989 34.923201293451896 15.925626515763943 15.76394502829426 7 33.225545675020214 31.204527081649154 17.623282134195634 17.946645109135005 8 30.533117932148627 33.60258481421648 17.12439418416801 18.73990306946688 9 28.53678253839935 18.270008084074373 19.078415521422798 34.114793856103475 10-11 28.253839935327406 25.828617623282135 25.747776879547292 20.16976556184317 12-13 29.951495553759095 25.464834276475344 23.96928051738076 20.6143896523848 14-15 26.63702506063056 25.666936135812453 22.190784155214228 25.505254648342763 16-17 27.4858528698464 27.607113985448667 24.535165723524656 20.371867421180276 18-19 27.72837510105093 25.424413904607924 26.27324171382377 20.57396928051738 20-21 25.747776879547292 26.39450282942603 27.64753435731609 20.21018593371059 22-23 26.879547291835088 25.464834276475344 26.313662085691185 21.341956345998383 24-25 25.22231204527082 25.26273241713824 27.930476960388035 21.58447857720291 26-27 26.27324171382377 25.666936135812453 26.7178658043654 21.341956345998383 28-29 25.747776879547292 25.464834276475344 26.27324171382377 22.514147130153596 30-31 25.464834276475344 26.39450282942603 27.243330638641876 20.89733225545675 32-33 26.475343573160874 26.232821341956345 24.818108326596604 22.473726758286176 34-35 26.79870654810024 25.545675020210183 24.93936944219887 22.716248989490705 36-37 26.839126919967665 25.666936135812453 27.40501212611156 20.088924818108328 38-39 27.04122877930477 24.413904607922394 25.545675020210183 22.999191592562653 40-41 26.63702506063056 26.758286176232822 24.77768795472918 21.827000808407437 42-43 25.949878738884397 25.505254648342763 26.434923201293454 22.109943411479385 44-45 25.807754442649433 25.92891760904685 26.575121163166397 21.688206785137318 46-47 25.68659127625202 27.261712439418417 25.0 22.051696284329562 48-49 25.605815831987076 26.777059773828753 26.696284329563813 20.920840064620354 50-51 26.21163166397415 26.615508885298865 25.363489499192244 21.809369951534734 52-53 26.736672051696285 25.92891760904685 26.17124394184168 21.163166397415186 54-55 23.87878787878788 26.86868686868687 26.505050505050505 22.747474747474747 56-57 25.92891760904685 26.65589660743134 25.44426494345719 21.97092084006462 58-59 25.848142164781905 24.878836833602584 25.20193861066236 24.07108239095315 60-61 24.03069466882068 27.423263327948305 25.20193861066236 23.34410339256866 62-63 26.534733441033925 26.009693053311793 24.636510500807756 22.819063004846527 64-65 24.31340872374798 28.150242326332798 25.08077544426494 22.45557350565428 66-67 25.767366720516964 24.232633279483036 27.18093699515347 22.819063004846527 68-69 25.333333333333336 25.49494949494949 25.292929292929294 23.87878787878788 70-71 24.00970088924818 26.354082457558608 25.22231204527082 24.413904607922394 72-73 26.151980598221503 25.3839935327405 24.6160064672595 23.848019401778497 74-75 25.333333333333336 26.707070707070706 23.393939393939394 24.565656565656564 76-77 25.373737373737377 26.3030303030303 23.67676767676768 24.646464646464647 78-79 25.605815831987076 26.009693053311793 24.232633279483036 24.151857835218095 80-81 26.373182552504037 25.96930533117932 24.757673667205168 22.89983844911147 82-83 27.504038772213246 25.484652665589664 23.505654281098547 23.505654281098547 84-85 27.705977382875606 25.88852988691438 24.757673667205168 21.647819063004846 86-87 27.665589660743134 24.79806138933764 25.161550888529888 22.37479806138934 88-89 26.63702506063056 26.071139854486663 24.130962004850446 23.16087308003234 90-91 26.7178658043654 26.03071948261924 23.443815683104287 23.807599029911074 92-93 26.192400970088926 24.37348423605497 24.252223120452708 25.181891673403396 94-95 26.071139854486663 23.60549717057397 24.6160064672595 25.70735650767987 96-97 25.949878738884397 25.464834276475344 24.00970088924818 24.575586095392076 98-99 25.02021018593371 25.666936135812453 25.02021018593371 24.292643492320128 100-101 26.607359482410025 25.19207440355843 22.765871411241406 25.434694702790132 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1.0 1 0.5 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 1.5 9 1.0 10 1.0 11 2.0 12 1.5 13 1.0 14 0.5 15 1.0 16 1.5 17 1.5 18 3.0 19 2.5 20 0.5 21 3.0 22 4.5 23 3.0 24 2.5 25 1.0 26 2.5 27 4.5 28 8.0 29 9.5 30 7.0 31 10.0 32 15.5 33 16.5 34 15.5 35 17.5 36 20.0 37 24.0 38 24.0 39 24.0 40 24.0 41 29.5 42 36.0 43 32.0 44 32.5 45 35.5 46 41.5 47 51.0 48 47.0 49 41.0 50 42.5 51 41.5 52 44.5 53 47.0 54 43.5 55 39.0 56 37.5 57 37.0 58 39.5 59 37.0 60 27.5 61 25.5 62 23.0 63 20.0 64 20.5 65 15.0 66 10.0 67 13.0 68 12.5 69 9.0 70 9.5 71 8.0 72 7.0 73 6.5 74 4.5 75 2.0 76 1.0 77 2.0 78 2.5 79 3.0 80 3.0 81 1.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.24232633279483037 2 0.08077544426494346 3 0.08077544426494346 4 0.08077544426494346 5 0.08077544426494346 6 0.08077544426494346 7 0.08077544426494346 8 0.0 9 0.08077544426494346 10-11 0.08077544426494346 12-13 0.08077544426494346 14-15 0.08077544426494346 16-17 0.08077544426494346 18-19 0.08077544426494346 20-21 0.08077544426494346 22-23 0.08077544426494346 24-25 0.08077544426494346 26-27 0.08077544426494346 28-29 0.08077544426494346 30-31 0.08077544426494346 32-33 0.08077544426494346 34-35 0.08077544426494346 36-37 0.08077544426494346 38-39 0.08077544426494346 40-41 0.08077544426494346 42-43 0.08077544426494346 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.04038772213247173 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.04038772213247173 70-71 0.08077544426494346 72-73 0.08077544426494346 74-75 0.04038772213247173 76-77 0.04038772213247173 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.08077544426494346 90-91 0.08077544426494346 92-93 0.08077544426494346 94-95 0.08077544426494346 96-97 0.08077544426494346 98-99 0.08077544426494346 100-101 0.12116316639741519 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1238.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 98.86914378029078 #Duplication Level Percentage of deduplicated Percentage of total 1 99.4281045751634 98.30371567043619 2 0.32679738562091504 0.6462035541195477 3 0.0 0.0 4 0.16339869281045752 0.6462035541195477 5 0.08169934640522876 0.40387722132471726 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.40387722132471726 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4 0.32310177705977383 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4 0.32310177705977383 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2 0.16155088852988692 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2 0.16155088852988692 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 2 0.16155088852988692 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2 0.16155088852988692 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.04038772213247173 0.0 38-39 0.0 0.0 0.0 0.08077544426494346 0.0 40-41 0.0 0.0 0.0 0.08077544426494346 0.0 42-43 0.0 0.0 0.0 0.08077544426494346 0.0 44-45 0.0 0.0 0.0 0.08077544426494346 0.0 46-47 0.0 0.0 0.0 0.08077544426494346 0.0 48-49 0.0 0.0 0.0 0.08077544426494346 0.0 50-51 0.0 0.0 0.0 0.2827140549273021 0.0 52-53 0.0 0.0 0.0 0.32310177705977383 0.0 54-55 0.0 0.0 0.0 0.32310177705977383 0.0 56-57 0.0 0.0 0.0 0.5250403877221325 0.0 58-59 0.0 0.0 0.0 0.8481421647819063 0.0 60-61 0.0 0.0 0.0 1.050080775444265 0.0 62-63 0.0 0.0 0.0 1.2116316639741518 0.0 64-65 0.0 0.0 0.0 1.2520193861066236 0.0 66-67 0.0 0.0 0.0 1.3731825525040389 0.0 68-69 0.0 0.0 0.0 1.4135702746365104 0.0 70-71 0.0 0.0 0.0 1.5751211631663975 0.0 72-73 0.0 0.0 0.0 1.7366720516962844 0.0 74-75 0.0 0.0 0.0 1.8578352180936994 0.0 76-77 0.0 0.0 0.0 1.8982229402261712 0.0 78-79 0.0 0.0 0.0 2.1405492730210014 0.0 80-81 0.0 0.0 0.0 2.3021001615508885 0.0 82-83 0.0 0.0 0.0 2.4636510500807756 0.0 84-85 0.0 0.0 0.0 2.6252019386106626 0.0 86-87 0.0 0.0 0.0 2.7867528271405493 0.0 88-89 0.0 0.0 0.0 3.02907915993538 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE