FastQCFastQC Report
Thu 26 May 2016
SRR938489_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938489_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1238
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG120.9693053311793215RNA PCR Primer, Index 39 (96% over 30bp)
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG110.8885298869143781RNA PCR Primer, Index 33 (96% over 29bp)
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG80.6462035541195477RNA PCR Primer, Index 23 (96% over 30bp)
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG70.5654281098546041RNA PCR Primer, Index 17 (96% over 28bp)
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG70.5654281098546041RNA PCR Primer, Index 31 (96% over 29bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.40387722132471726No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.40387722132471726No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG50.40387722132471726TruSeq Adapter, Index 10 (96% over 28bp)
GGATTTAAAAACTGGAACGGTGAAGGCGACAGCAGTTGGTTGGAGCAAAC40.32310177705977383No Hit
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGA40.32310177705977383RNA PCR Primer, Index 33 (96% over 29bp)
ATACTGAACTGCTACGTAACACAGTTCCACCCGCCTCACATTGAAATCCA30.24232633279483037No Hit
ACTCAGACTACCCAGATGCTTACACCACATGAAACACTGTCTCTTCTATA30.24232633279483037No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG30.24232633279483037RNA PCR Primer, Index 26 (100% over 29bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.24232633279483037No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG30.24232633279483037RNA PCR Primer, Index 27 (96% over 30bp)
GTTGAGGGCCTCTGTCCTGGACAGGGAGATGACGACATTGGGCTGTTCAA20.16155088852988692No Hit
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGA20.16155088852988692RNA PCR Primer, Index 46 (96% over 28bp)
ATGCTACACATTGGGAGCACATCCATGTCGGGTTGGAAAAGACAGTCCTC20.16155088852988692No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.16155088852988692No Hit
GTCTCTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGAT20.16155088852988692No Hit
CTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTGA20.16155088852988692RNA PCR Primer, Index 23 (96% over 30bp)
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGA20.16155088852988692RNA PCR Primer, Index 10 (96% over 28bp)
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.16155088852988692No Hit
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGA20.16155088852988692RNA PCR Primer, Index 39 (96% over 30bp)
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG20.16155088852988692TruSeq Adapter, Index 3 (96% over 28bp)
CCCAAAAGCCACCCCCACTCCTAAGAGGAGGATGGTCGCGTCCATGCCCT20.16155088852988692No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA20.16155088852988692No Hit
GCCCCCGATGCCGCTCAACATCTTGCTCCAGGCAGACTCAGACTGTGCCC20.16155088852988692No Hit
TCTCCGAGCCCACGAGACGCTACGCTATCTCGGATGCCGTCTTCTGCTTG20.16155088852988692RNA PCR Primer, Index 38 (96% over 27bp)
GGACCTGGAAGGTCCAGTCCCCATTCCTAATAAGCTGTGTGGATGAGACC20.16155088852988692No Hit
GCCAGGGCTGGCCTGTACACTGACTTGAGACCAATAAAAGTGCACACCTT20.16155088852988692No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA20.16155088852988692No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG20.16155088852988692No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG20.16155088852988692TruSeq Adapter, Index 1 (96% over 28bp)
GTCACTGAGCAGACCAGAGTGTTGTGGTGGTTGAGGGCCTCTGTCCTGGA20.16155088852988692No Hit
GTCCCTCACCCTCCCAAAAGCCACCCCCACTCCTAAGAGGAGGATGGTCG20.16155088852988692No Hit
CTTCTTCGCTATCTGTGTCTTTCATTTTTCTAGCCATCATAGTCAAGAAC20.16155088852988692No Hit
CATCCATGTCGGGTTGGAAAAGACAGTCCTCGCTGTCGGGAAAACAGGGA20.16155088852988692No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers