##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938489_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1238 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.467689822294023 31.0 30.0 31.0 27.0 34.0 2 30.79806138933764 31.0 30.0 31.0 27.0 34.0 3 30.84410339256866 31.0 30.0 33.0 27.0 34.0 4 34.625201938610665 35.0 35.0 37.0 32.0 37.0 5 34.35056542810985 35.0 35.0 37.0 30.0 37.0 6 34.30210016155089 35.0 33.0 37.0 30.0 37.0 7 34.07108239095315 35.0 33.0 37.0 30.0 37.0 8 33.903069466882066 35.0 33.0 37.0 29.0 37.0 9 35.362681744749594 37.0 34.0 39.0 30.0 39.0 10-11 35.43214862681745 37.0 34.0 39.0 29.5 39.0 12-13 35.32915993537965 37.0 34.0 39.0 29.5 39.0 14-15 35.72899838449112 38.0 33.5 40.0 27.0 41.0 16-17 35.5375605815832 37.5 33.0 40.0 27.0 41.0 18-19 35.72092084006462 38.0 33.5 40.0 28.5 41.0 20-21 35.6829563812601 38.0 34.0 40.0 27.5 41.0 22-23 35.70840064620356 38.0 34.0 40.0 28.0 41.0 24-25 35.350969305331176 38.0 33.0 40.0 27.0 41.0 26-27 34.8218901453958 37.5 32.5 40.0 25.5 41.0 28-29 34.42084006462035 37.0 32.0 40.0 24.5 41.0 30-31 34.50444264943457 37.0 32.0 40.0 25.0 41.0 32-33 34.07875605815832 36.5 32.0 39.0 24.5 40.5 34-35 33.90064620355412 37.0 31.5 39.5 24.0 40.5 36-37 33.987479806138936 37.0 32.0 39.5 24.0 41.0 38-39 33.544426494345714 37.0 31.5 40.0 23.0 41.0 40-41 33.00403877221325 36.0 31.0 39.0 20.5 40.0 42-43 33.016558966074314 36.5 31.0 39.0 21.0 40.5 44-45 32.83319870759289 36.0 31.0 39.0 20.0 40.5 46-47 32.09208400646204 36.0 30.0 39.0 15.0 40.0 48-49 31.65751211631664 35.5 30.0 39.0 13.0 40.0 50-51 30.749192245557353 35.0 28.0 38.0 8.0 40.0 52-53 30.170436187399034 34.0 27.0 38.0 4.5 40.0 54-55 29.238691437802906 34.0 26.0 38.0 2.0 40.0 56-57 28.1062197092084 33.0 23.0 38.0 2.0 40.0 58-59 27.253634894991922 33.5 20.5 37.5 2.0 40.0 60-61 26.531906300484653 32.5 19.0 37.0 2.0 40.0 62-63 25.956785137318256 32.0 16.0 36.5 2.0 39.0 64-65 25.447495961227787 31.0 13.0 36.0 2.0 39.0 66-67 24.562197092084006 31.0 8.0 35.5 2.0 39.0 68-69 24.111873990306947 30.0 7.5 35.0 2.0 38.0 70-71 23.734248788368333 30.0 4.5 35.0 2.0 38.0 72-73 22.905492730210014 29.0 2.0 34.0 2.0 37.0 74-75 22.384087237479804 29.0 2.0 34.0 2.0 36.0 76-77 21.487479806138936 27.0 2.0 32.5 2.0 35.0 78-79 21.870355411954765 28.5 2.0 34.0 2.0 35.5 80-81 21.644184168012924 29.0 2.0 34.0 2.0 35.5 82-83 21.180936995153473 29.0 2.0 34.0 2.0 35.0 84-85 20.640549273021 27.0 2.0 34.0 2.0 35.0 86-87 20.03069466882068 26.0 2.0 33.5 2.0 35.0 88-89 19.334410339256866 26.0 2.0 33.0 2.0 35.0 90-91 18.283117932148627 24.5 2.0 32.0 2.0 34.5 92-93 17.74394184168013 23.5 2.0 32.0 2.0 34.0 94-95 16.714458804523424 18.5 2.0 32.0 2.0 34.0 96-97 15.967285945072698 6.0 2.0 32.0 2.0 34.0 98-99 14.733037156704363 2.0 2.0 31.0 2.0 34.0 100-101 12.845718901453958 2.0 2.0 28.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1102 1 -1.683150183150186 1102 2 0.09890109890109855 1102 3 -0.3919413919413941 1102 4 1.6355311355311315 1102 5 1.8608058608058542 1102 6 -3.258241758241759 1102 7 -3.2600732600732627 1102 8 -0.1611721611721677 1102 9 1.8901098901098905 1102 10-11 -0.3580586080586059 1102 12-13 1.9001831501831532 1102 14-15 1.124542124542124 1102 16-17 -2.0668498168498104 1102 18-19 -4.703296703296701 1102 20-21 -1.8864468864468904 1102 22-23 -4.050366300366299 1102 24-25 -8.083333333333329 1102 26-27 3.0705128205128247 1102 28-29 -3.664835164835168 1102 30-31 -7.728021978021978 1102 32-33 -7.31684981684981 1102 34-35 -7.07326007326008 1102 36-37 -8.583333333333329 1102 38-39 -11.46520146520146 1102 40-41 -4.812271062271066 1102 42-43 -4.051282051282051 1102 44-45 -2.969780219780219 1102 46-47 -0.633699633699635 1102 48-49 2.1382783882783833 1102 50-51 -1.1428571428571423 1102 52-53 2.705128205128208 1102 54-55 -8.315934065934066 1102 56-57 -5.057692307692307 1102 58-59 -7.872710622710628 1102 60-61 -3.245421245421241 1102 62-63 -2.1620879120879124 1102 64-65 -1.7930402930402956 1102 66-67 6.1593406593406606 1102 68-69 3.1190476190476204 1102 70-71 9.153846153846153 1102 72-73 9.942307692307693 1102 74-75 7.71336996336996 1102 76-77 7.574175824175825 1102 78-79 7.423992673992675 1102 80-81 8.871794871794872 1102 82-83 9.22252747252747 1102 84-85 10.488095238095237 1102 86-87 13.51007326007326 1102 88-89 6.070512820512818 1102 90-91 -1.0439560439560438 1102 92-93 -1.9844322344322372 1102 94-95 -6.537545787545785 1102 96-97 -3.3498168498168504 1102 98-99 0.1446886446886424 1102 100-101 2.6730769230769234 1103 1 0.31684981684981395 1103 2 -0.40109890109890145 1103 3 0.1080586080586059 1103 4 1.6355311355311315 1103 5 2.860805860805854 1103 6 -1.258241758241759 1103 7 1.2399267399267373 1103 8 0.8388278388278323 1103 9 2.3901098901098905 1103 10-11 1.141941391941394 1103 12-13 1.9001831501831532 1103 14-15 -0.1254578754578759 1103 16-17 -2.0668498168498104 1103 18-19 1.0467032967032992 1103 20-21 0.1135531135531096 1103 22-23 0.6996336996337007 1103 24-25 1.9166666666666714 1103 26-27 3.3205128205128247 1103 28-29 2.085164835164832 1103 30-31 2.021978021978022 1103 32-33 5.43315018315019 1103 34-35 -2.07326007326008 1103 36-37 -6.083333333333329 1103 38-39 0.28479853479854 1103 40-41 6.187728937728934 1103 42-43 1.698717948717949 1103 44-45 5.280219780219781 1103 46-47 1.866300366300365 1103 48-49 6.138278388278383 1103 50-51 4.107142857142858 1103 52-53 3.455128205128208 1103 54-55 3.6840659340659343 1103 56-57 -2.3076923076923066 1103 58-59 8.627289377289372 1103 60-61 11.254578754578759 1103 62-63 -1.6620879120879124 1103 64-65 2.2069597069597044 1103 66-67 1.9093406593406606 1103 68-69 1.6190476190476204 1103 70-71 1.6538461538461533 1103 72-73 8.692307692307693 1103 74-75 2.96336996336996 1103 76-77 8.574175824175825 1103 78-79 8.673992673992675 1103 80-81 12.121794871794872 1103 82-83 11.72252747252747 1103 84-85 8.738095238095237 1103 86-87 3.010073260073259 1103 88-89 6.820512820512818 1103 90-91 0.2060439560439562 1103 92-93 -3.484432234432237 1103 94-95 -3.5375457875457847 1103 96-97 -10.09981684981685 1103 98-99 -6.855311355311358 1103 100-101 1.4230769230769234 1104 1 0.31684981684981395 1104 2 3.0989010989010985 1104 3 0.1080586080586059 1104 4 0.6355311355311315 1104 5 -1.1391941391941458 1104 6 1.241758241758241 1104 7 3.2399267399267373 1104 8 2.8388278388278323 1104 9 3.3901098901098905 1104 10-11 0.6419413919413941 1104 12-13 0.9001831501831532 1104 14-15 2.124542124542124 1104 16-17 1.1831501831501896 1104 18-19 3.046703296703299 1104 20-21 3.6135531135531096 1104 22-23 2.4496336996337007 1104 24-25 -1.5833333333333286 1104 26-27 3.8205128205128247 1104 28-29 4.835164835164832 1104 30-31 3.271978021978022 1104 32-33 -3.3168498168498104 1104 34-35 3.67673992673992 1104 36-37 4.666666666666671 1104 38-39 5.03479853479854 1104 40-41 6.937728937728934 1104 42-43 4.948717948717949 1104 44-45 -3.219780219780219 1104 46-47 1.866300366300365 1104 48-49 -4.111721611721617 1104 50-51 -0.6428571428571423 1104 52-53 1.705128205128208 1104 54-55 7.684065934065934 1104 56-57 -4.057692307692307 1104 58-59 1.3772893772893724 1104 60-61 9.504578754578759 1104 62-63 8.337912087912088 1104 64-65 8.456959706959704 1104 66-67 8.15934065934066 1104 68-69 6.86904761904762 1104 70-71 8.403846153846153 1104 72-73 10.942307692307693 1104 74-75 12.71336996336996 1104 76-77 12.324175824175825 1104 78-79 12.673992673992675 1104 80-81 12.121794871794872 1104 82-83 9.97252747252747 1104 84-85 13.488095238095237 1104 86-87 -4.239926739926741 1104 88-89 7.320512820512818 1104 90-91 11.456043956043956 1104 92-93 11.515567765567763 1104 94-95 16.962454212454215 1104 96-97 12.15018315018315 1104 98-99 11.894688644688642 1104 100-101 13.423076923076923 1105 1 -0.683150183150186 1105 2 -0.9010989010989015 1105 3 -0.8919413919413941 1105 4 0.6355311355311315 1105 5 0.8608058608058542 1105 6 0.2417582417582409 1105 7 1.2399267399267373 1105 8 -7.161172161172168 1105 9 -18.60989010989011 1105 10-11 -13.358058608058606 1105 12-13 -12.599816849816847 1105 14-15 -5.375457875457876 1105 16-17 -11.31684981684981 1105 18-19 -4.953296703296701 1105 20-21 -0.8864468864468904 1105 22-23 -1.0503663003662993 1105 24-25 -1.5833333333333286 1105 26-27 -1.6794871794871753 1105 28-29 0.3351648351648322 1105 30-31 -2.728021978021978 1105 32-33 -4.81684981684981 1105 34-35 -9.32326007326008 1105 36-37 -2.3333333333333286 1105 38-39 0.034798534798540004 1105 40-41 -1.0622710622710656 1105 42-43 0.9487179487179489 1105 44-45 1.780219780219781 1105 46-47 -11.133699633699635 1105 48-49 -10.611721611721617 1105 50-51 -15.642857142857142 1105 52-53 0.20512820512820795 1105 54-55 5.184065934065934 1105 56-57 2.4423076923076934 1105 58-59 -18.122710622710628 1105 60-61 1.504578754578759 1105 62-63 1.8379120879120876 1105 64-65 5.4569597069597044 1105 66-67 8.65934065934066 1105 68-69 4.86904761904762 1105 70-71 -21.596153846153847 1105 72-73 -21.057692307692307 1105 74-75 -20.28663003663004 1105 76-77 -19.675824175824175 1105 78-79 -19.826007326007325 1105 80-81 -19.878205128205128 1105 82-83 -19.52747252747253 1105 84-85 -18.511904761904763 1105 86-87 -18.23992673992674 1105 88-89 -18.679487179487182 1105 90-91 -18.043956043956044 1105 92-93 -16.984432234432237 1105 94-95 -15.537545787545785 1105 96-97 -15.84981684981685 1105 98-99 -14.605311355311358 1105 100-101 -12.576923076923077 1106 1 3.316849816849814 1106 2 0.09890109890109855 1106 3 3.108058608058606 1106 4 2.6355311355311315 1106 5 2.860805860805854 1106 6 2.241758241758241 1106 7 3.2399267399267373 1106 8 2.8388278388278323 1106 9 3.3901098901098905 1106 10-11 3.141941391941394 1106 12-13 3.400183150183153 1106 14-15 3.624542124542124 1106 16-17 0.1831501831501896 1106 18-19 2.546703296703299 1106 20-21 3.6135531135531096 1106 22-23 1.9496336996337007 1106 24-25 3.9166666666666714 1106 26-27 6.320512820512825 1106 28-29 3.835164835164832 1106 30-31 5.771978021978022 1106 32-33 7.18315018315019 1106 34-35 6.17673992673992 1106 36-37 6.666666666666671 1106 38-39 6.03479853479854 1106 40-41 6.937728937728934 1106 42-43 5.448717948717949 1106 44-45 3.780219780219781 1106 46-47 7.866300366300365 1106 48-49 6.388278388278383 1106 50-51 9.357142857142858 1106 52-53 10.705128205128208 1106 54-55 11.184065934065934 1106 56-57 11.442307692307693 1106 58-59 11.877289377289372 1106 60-61 14.004578754578759 1106 62-63 14.337912087912088 1106 64-65 13.456959706959704 1106 66-67 12.65934065934066 1106 68-69 14.36904761904762 1106 70-71 16.903846153846153 1106 72-73 8.942307692307693 1106 74-75 15.21336996336996 1106 76-77 14.824175824175825 1106 78-79 16.173992673992675 1106 80-81 14.121794871794872 1106 82-83 10.97252747252747 1106 84-85 14.488095238095237 1106 86-87 14.76007326007326 1106 88-89 10.320512820512818 1106 90-91 11.956043956043956 1106 92-93 13.515567765567763 1106 94-95 10.462454212454215 1106 96-97 15.65018315018315 1106 98-99 15.394688644688642 1106 100-101 13.423076923076923 1107 1 -0.683150183150186 1107 2 0.09890109890109855 1107 3 2.108058608058606 1107 4 2.6355311355311315 1107 5 2.860805860805854 1107 6 2.241758241758241 1107 7 -25.760073260073263 1107 8 -1.1611721611721677 1107 9 1.3901098901098905 1107 10-11 1.641941391941394 1107 12-13 2.900183150183153 1107 14-15 5.124542124542124 1107 16-17 4.68315018315019 1107 18-19 5.046703296703299 1107 20-21 4.61355311355311 1107 22-23 3.9496336996337007 1107 24-25 4.916666666666671 1107 26-27 6.320512820512825 1107 28-29 5.335164835164832 1107 30-31 3.771978021978022 1107 32-33 5.18315018315019 1107 34-35 6.17673992673992 1107 36-37 7.166666666666671 1107 38-39 7.03479853479854 1107 40-41 6.937728937728934 1107 42-43 -0.5512820512820511 1107 44-45 4.780219780219781 1107 46-47 5.866300366300365 1107 48-49 8.888278388278383 1107 50-51 8.857142857142858 1107 52-53 10.705128205128208 1107 54-55 10.184065934065934 1107 56-57 9.942307692307693 1107 58-59 10.877289377289372 1107 60-61 12.504578754578759 1107 62-63 12.337912087912088 1107 64-65 13.956959706959704 1107 66-67 10.15934065934066 1107 68-69 11.36904761904762 1107 70-71 14.403846153846153 1107 72-73 14.942307692307693 1107 74-75 10.71336996336996 1107 76-77 5.824175824175825 1107 78-79 11.173992673992675 1107 80-81 11.621794871794872 1107 82-83 8.47252747252747 1107 84-85 13.988095238095237 1107 86-87 14.26007326007326 1107 88-89 14.320512820512818 1107 90-91 12.956043956043956 1107 92-93 15.515567765567763 1107 94-95 16.462454212454215 1107 96-97 15.15018315018315 1107 98-99 13.394688644688642 1107 100-101 14.923076923076923 1108 1 0.31684981684981395 1108 2 0.09890109890109855 1108 3 0.1080586080586059 1108 4 0.6355311355311315 1108 5 0.8608058608058542 1108 6 2.241758241758241 1108 7 3.2399267399267373 1108 8 2.8388278388278323 1108 9 2.3901098901098905 1108 10-11 -4.858058608058606 1108 12-13 0.9001831501831532 1108 14-15 1.624542124542124 1108 16-17 3.1831501831501896 1108 18-19 3.046703296703299 1108 20-21 1.1135531135531096 1108 22-23 2.4496336996337007 1108 24-25 -6.583333333333329 1108 26-27 -3.1794871794871753 1108 28-29 -0.6648351648351678 1108 30-31 3.271978021978022 1108 32-33 -1.3168498168498104 1108 34-35 1.17673992673992 1108 36-37 -0.3333333333333286 1108 38-39 0.034798534798540004 1108 40-41 2.9377289377289344 1108 42-43 3.948717948717949 1108 44-45 5.280219780219781 1108 46-47 4.366300366300365 1108 48-49 0.8882783882783833 1108 50-51 -2.6428571428571423 1108 52-53 0.20512820512820795 1108 54-55 -13.315934065934066 1108 56-57 -27.057692307692307 1108 58-59 -26.122710622710628 1108 60-61 -24.49542124542124 1108 62-63 -24.662087912087912 1108 64-65 -25.043040293040296 1108 66-67 -24.34065934065934 1108 68-69 -22.63095238095238 1108 70-71 -21.596153846153847 1108 72-73 -21.057692307692307 1108 74-75 -20.28663003663004 1108 76-77 -19.675824175824175 1108 78-79 -19.826007326007325 1108 80-81 -19.878205128205128 1108 82-83 -19.52747252747253 1108 84-85 -18.511904761904763 1108 86-87 -18.23992673992674 1108 88-89 -18.679487179487182 1108 90-91 -18.043956043956044 1108 92-93 -16.984432234432237 1108 94-95 -15.537545787545785 1108 96-97 -15.84981684981685 1108 98-99 -14.605311355311358 1108 100-101 -12.576923076923077 1109 1 0.31684981684981395 1109 2 3.0989010989010985 1109 3 3.108058608058606 1109 4 2.6355311355311315 1109 5 2.860805860805854 1109 6 2.241758241758241 1109 7 1.2399267399267373 1109 8 2.8388278388278323 1109 9 3.3901098901098905 1109 10-11 3.641941391941394 1109 12-13 3.900183150183153 1109 14-15 5.124542124542124 1109 16-17 5.18315018315019 1109 18-19 5.046703296703299 1109 20-21 4.11355311355311 1109 22-23 4.949633699633701 1109 24-25 5.416666666666671 1109 26-27 6.320512820512825 1109 28-29 5.835164835164832 1109 30-31 6.271978021978022 1109 32-33 7.18315018315019 1109 34-35 7.17673992673992 1109 36-37 6.166666666666671 1109 38-39 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-15.84981684981685 2316 98-99 -14.605311355311358 2316 100-101 -12.576923076923077 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 0.0 7 4.0 8 2.0 9 3.0 10 3.0 11 12.0 12 16.0 13 15.0 14 26.0 15 25.0 16 31.0 17 32.0 18 36.0 19 20.0 20 45.0 21 29.0 22 40.0 23 30.0 24 27.0 25 38.0 26 32.0 27 39.0 28 51.0 29 51.0 30 70.0 31 71.0 32 69.0 33 70.0 34 91.0 35 68.0 36 94.0 37 74.0 38 23.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.25686591276252 18.174474959612276 18.578352180936992 23.990306946688207 2 16.814874696847212 17.13823767178658 37.51010509296685 28.53678253839935 3 16.882067851373183 22.536348949919223 34.087237479806134 26.494345718901453 4 12.681744749596122 15.266558966074314 33.60258481421648 38.44911147011308 5 13.419563459983832 34.438156831042846 31.447049312853682 20.695230396119644 6 36.187399030694664 33.68336025848142 17.205169628432955 12.924071082390952 7 29.079159935379646 34.5718901453958 21.08239095315024 15.266558966074314 8 29.40226171243942 32.7140549273021 16.397415185783522 21.486268174474958 9 22.617124394184167 17.12439418416801 19.951534733441033 40.306946688206786 10-11 23.101777059773827 25.24232633279483 24.636510500807756 27.01938610662359 12-13 23.1421647819063 24.757673667205168 26.13085621970921 25.96930533117932 14-15 25.726978998384492 25.484652665589664 24.919224555735056 23.86914378029079 16-17 23.1421647819063 28.554119547657514 25.767366720516964 22.536348949919223 18-19 20.880452342487885 24.394184168012924 28.392568659127626 26.33279483037157 20-21 22.819063004846527 25.848142164781905 28.513731825525042 22.819063004846527 22-23 22.819063004846527 25.121163166397416 28.91760904684976 23.1421647819063 24-25 23.424878836833603 25.767366720516964 28.473344103392566 22.334410339256866 26-27 21.809369951534734 27.907915993537962 27.62520193861066 22.65751211631664 28-29 20.234248788368337 24.959612277867528 30.210016155088855 24.596122778675284 30-31 21.768982229402262 24.838449111470112 28.75605815831987 24.636510500807756 32-33 23.06138933764136 25.96930533117932 29.604200323101775 21.365105008077546 34-35 20.516962843295637 27.665589660743134 30.048465266558967 21.768982229402262 36-37 24.55573505654281 22.738287560581583 27.30210016155089 25.403877221324716 38-39 24.31340872374798 24.35379644588045 27.261712439418417 24.07108239095315 40-41 21.122778675282714 24.474959612277868 31.583198707592892 22.819063004846527 42-43 20.032310177705977 23.06138933764136 34.0064620355412 22.89983844911147 44-45 21.001615508885298 23.82875605815832 31.906300484652668 23.263327948303715 46-47 24.232633279483036 24.636510500807756 26.090468497576737 25.040387722132472 48-49 21.607431340872374 24.232633279483036 31.94668820678514 22.21324717285945 50-51 23.626817447495963 29.483037156704363 24.919224555735056 21.97092084006462 52-53 20.476575121163165 32.4313408723748 25.44426494345719 21.647819063004846 54-55 20.638126009693053 31.623586429725364 26.615508885298865 21.122778675282714 56-57 19.991922455573505 31.94668820678514 25.161550888529888 22.89983844911147 58-59 20.96122778675283 32.67366720516963 24.596122778675284 21.768982229402262 60-61 22.051696284329562 28.67528271405493 27.382875605815833 21.890145395799678 62-63 20.920840064620354 28.190630048465266 26.777059773828753 24.111470113085623 64-65 20.79967689822294 29.159935379644587 25.767366720516964 24.273021001615508 66-67 21.890145395799678 28.513731825525042 25.96930533117932 23.626817447495963 68-69 22.576736672051698 29.483037156704363 24.919224555735056 23.021001615508887 70-71 21.52665589660743 29.321486268174475 25.68659127625202 23.465266558966075 72-73 24.03069466882068 27.746365105008074 26.453957996768985 21.768982229402262 74-75 21.405492730210014 28.31179321486268 26.413570274636513 23.86914378029079 76-77 23.34410339256866 27.62520193861066 25.726978998384492 23.303715670436187 78-79 23.06138933764136 28.877221324717283 26.81744749596123 21.24394184168013 80-81 23.1421647819063 26.21163166397415 26.090468497576737 24.55573505654281 82-83 22.092084006462034 27.54442649434572 26.373182552504037 23.990306946688207 84-85 21.849757673667206 27.746365105008074 27.01938610662359 23.38449111470113 86-87 22.37479806138934 27.62520193861066 25.88852988691438 24.111470113085623 88-89 22.940226171243943 26.777059773828753 26.453957996768985 23.82875605815832 90-91 21.647819063004846 28.029079159935378 26.65589660743134 23.66720516962843 92-93 22.576736672051698 27.705977382875606 25.92891760904685 23.788368336025847 94-95 23.06138933764136 25.88852988691438 26.17124394184168 24.878836833602584 96-97 20.59773828756058 27.705977382875606 27.30210016155089 24.394184168012924 98-99 20.75928917609047 26.292407108239097 27.463651050080774 25.484652665589664 100-101 21.647819063004846 25.848142164781905 25.96930533117932 26.534733441033925 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.5 3 0.5 4 0.0 5 0.5 6 1.0 7 3.0 8 2.5 9 0.5 10 1.0 11 0.5 12 0.5 13 1.0 14 1.5 15 1.5 16 0.5 17 0.5 18 1.0 19 2.0 20 2.5 21 3.0 22 3.5 23 2.5 24 3.0 25 3.5 26 4.0 27 7.0 28 10.0 29 8.0 30 7.5 31 12.0 32 17.5 33 21.5 34 22.0 35 25.0 36 28.0 37 27.5 38 25.5 39 26.5 40 27.0 41 38.0 42 46.0 43 44.5 44 45.5 45 44.5 46 46.5 47 49.0 48 51.5 49 49.5 50 48.0 51 57.5 52 55.0 53 41.0 54 42.0 55 45.0 56 40.0 57 34.0 58 31.0 59 26.0 60 21.5 61 18.5 62 13.5 63 10.0 64 8.0 65 5.5 66 3.0 67 3.5 68 3.5 69 2.0 70 2.0 71 2.0 72 1.5 73 1.5 74 1.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.5 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.08077544426494346 3 0.0 4 0.0 5 0.08077544426494346 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1238.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 92.64943457189014 #Duplication Level Percentage of deduplicated Percentage of total 1 96.68700959023539 89.5799676898223 2 2.0052310374891023 3.715670436187399 3 0.4359197907585004 1.2116316639741518 4 0.17436791630340018 0.6462035541195477 5 0.26155187445510025 1.2116316639741518 6 0.0 0.0 7 0.17436791630340018 1.1308562197092082 8 0.08718395815170009 0.6462035541195477 9 0.0 0.0 >10 0.17436791630340018 1.8578352180936994 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG 12 0.9693053311793215 RNA PCR Primer, Index 39 (96% over 30bp) TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG 11 0.8885298869143781 RNA PCR Primer, Index 33 (96% over 29bp) TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG 8 0.6462035541195477 RNA PCR Primer, Index 23 (96% over 30bp) TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG 7 0.5654281098546041 RNA PCR Primer, Index 17 (96% over 28bp) TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG 7 0.5654281098546041 RNA PCR Primer, Index 31 (96% over 29bp) GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.40387722132471726 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.40387722132471726 No Hit TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG 5 0.40387722132471726 TruSeq Adapter, Index 10 (96% over 28bp) GGATTTAAAAACTGGAACGGTGAAGGCGACAGCAGTTGGTTGGAGCAAAC 4 0.32310177705977383 No Hit CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGA 4 0.32310177705977383 RNA PCR Primer, Index 33 (96% over 29bp) ATACTGAACTGCTACGTAACACAGTTCCACCCGCCTCACATTGAAATCCA 3 0.24232633279483037 No Hit ACTCAGACTACCCAGATGCTTACACCACATGAAACACTGTCTCTTCTATA 3 0.24232633279483037 No Hit TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG 3 0.24232633279483037 RNA PCR Primer, Index 26 (100% over 29bp) TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3 0.24232633279483037 No Hit TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG 3 0.24232633279483037 RNA PCR Primer, Index 27 (96% over 30bp) GTTGAGGGCCTCTGTCCTGGACAGGGAGATGACGACATTGGGCTGTTCAA 2 0.16155088852988692 No Hit CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGA 2 0.16155088852988692 RNA PCR Primer, Index 46 (96% over 28bp) ATGCTACACATTGGGAGCACATCCATGTCGGGTTGGAAAAGACAGTCCTC 2 0.16155088852988692 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2 0.16155088852988692 No Hit GTCTCTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGAT 2 0.16155088852988692 No Hit CTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTGA 2 0.16155088852988692 RNA PCR Primer, Index 23 (96% over 30bp) CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGA 2 0.16155088852988692 RNA PCR Primer, Index 10 (96% over 28bp) TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2 0.16155088852988692 No Hit CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGA 2 0.16155088852988692 RNA PCR Primer, Index 39 (96% over 30bp) TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG 2 0.16155088852988692 TruSeq Adapter, Index 3 (96% over 28bp) CCCAAAAGCCACCCCCACTCCTAAGAGGAGGATGGTCGCGTCCATGCCCT 2 0.16155088852988692 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 2 0.16155088852988692 No Hit GCCCCCGATGCCGCTCAACATCTTGCTCCAGGCAGACTCAGACTGTGCCC 2 0.16155088852988692 No Hit TCTCCGAGCCCACGAGACGCTACGCTATCTCGGATGCCGTCTTCTGCTTG 2 0.16155088852988692 RNA PCR Primer, Index 38 (96% over 27bp) GGACCTGGAAGGTCCAGTCCCCATTCCTAATAAGCTGTGTGGATGAGACC 2 0.16155088852988692 No Hit GCCAGGGCTGGCCTGTACACTGACTTGAGACCAATAAAAGTGCACACCTT 2 0.16155088852988692 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2 0.16155088852988692 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2 0.16155088852988692 No Hit TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG 2 0.16155088852988692 TruSeq Adapter, Index 1 (96% over 28bp) GTCACTGAGCAGACCAGAGTGTTGTGGTGGTTGAGGGCCTCTGTCCTGGA 2 0.16155088852988692 No Hit GTCCCTCACCCTCCCAAAAGCCACCCCCACTCCTAAGAGGAGGATGGTCG 2 0.16155088852988692 No Hit CTTCTTCGCTATCTGTGTCTTTCATTTTTCTAGCCATCATAGTCAAGAAC 2 0.16155088852988692 No Hit CATCCATGTCGGGTTGGAAAAGACAGTCCTCGCTGTCGGGAAAACAGGGA 2 0.16155088852988692 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.04038772213247173 0.0 38-39 0.0 0.0 0.0 0.08077544426494346 0.0 40-41 0.0 0.0 0.0 0.08077544426494346 0.0 42-43 0.0 0.0 0.0 0.08077544426494346 0.0 44-45 0.0 0.0 0.0 0.08077544426494346 0.0 46-47 0.0 0.0 0.0 0.08077544426494346 0.0 48-49 0.0 0.0 0.0 0.08077544426494346 0.0 50-51 0.0 0.0 0.0 0.24232633279483037 0.0 52-53 0.0 0.0 0.0 0.32310177705977383 0.0 54-55 0.0 0.0 0.0 0.32310177705977383 0.0 56-57 0.0 0.0 0.0 0.5654281098546041 0.0 58-59 0.0 0.0 0.0 0.8077544426494345 0.0 60-61 0.0 0.0 0.0 1.050080775444265 0.0 62-63 0.0 0.0 0.0 1.2520193861066236 0.0 64-65 0.0 0.0 0.0 1.4135702746365104 0.0 66-67 0.0 0.0 0.0 1.615508885298869 0.0 68-69 0.0 0.0 0.0 1.7366720516962844 0.0 70-71 0.0 0.0 0.0 1.8174474959612277 0.0 72-73 0.0 0.0 0.0 1.9386106623586428 0.0 74-75 0.0 0.0 0.0 2.1809369951534734 0.0 76-77 0.0 0.0 0.0 2.2213247172859454 0.0 78-79 0.0 0.0 0.0 2.4636510500807756 0.0 80-81 0.0 0.0 0.0 2.6252019386106626 0.0 82-83 0.0 0.0 0.0 2.7867528271405493 0.0 84-85 0.0 0.0 0.0 2.988691437802908 0.0 86-87 0.0 0.0 0.0 3.150242326332795 0.0 88-89 0.0 0.0 0.0 3.432956381260097 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE