##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938488_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1264 Sequences flagged as poor quality 0 Sequence length 101 %GC 49 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.469145569620252 26.0 10.0 31.0 2.0 33.0 2 20.85126582278481 26.0 10.0 31.0 2.0 33.0 3 20.852848101265824 27.0 10.0 31.0 2.0 34.0 4 23.189082278481013 30.0 10.0 35.0 2.0 37.0 5 22.727848101265824 30.0 2.0 35.0 2.0 37.0 6 22.814873417721518 30.0 2.0 35.0 2.0 37.0 7 22.32278481012658 30.0 2.0 35.0 2.0 37.0 8 22.488132911392405 30.0 2.0 35.0 2.0 37.0 9 22.783227848101266 30.0 2.0 37.0 2.0 39.0 10-11 22.439082278481013 30.0 2.0 37.0 2.0 39.0 12-13 21.90743670886076 28.5 2.0 37.0 2.0 39.0 14-15 21.814873417721518 27.0 2.0 37.0 2.0 40.0 16-17 21.371835443037973 27.0 2.0 37.0 2.0 40.0 18-19 21.140427215189874 27.0 2.0 37.5 2.0 40.0 20-21 21.01107594936709 27.0 2.0 37.0 2.0 40.0 22-23 20.720727848101266 26.0 2.0 37.0 2.0 40.0 24-25 20.384493670886076 25.0 2.0 37.0 2.0 40.0 26-27 19.877768987341774 24.0 2.0 37.0 2.0 40.0 28-29 19.55142405063291 23.0 2.0 37.0 2.0 40.0 30-31 19.17286392405063 18.5 2.0 37.0 2.0 40.0 32-33 18.812104430379748 17.0 2.0 36.0 2.0 39.0 34-35 18.42642405063291 15.5 2.0 35.0 2.0 39.0 36-37 18.035996835443036 14.0 2.0 35.5 2.0 39.5 38-39 17.687895569620252 9.0 2.0 35.0 2.0 39.5 40-41 17.045094936708864 8.0 2.0 34.5 2.0 39.0 42-43 16.706882911392405 2.0 2.0 34.5 2.0 39.0 44-45 16.145569620253163 2.0 2.0 34.0 2.0 38.5 46-47 16.209651898734176 2.0 2.0 34.5 2.0 38.5 48-49 15.564477848101266 2.0 2.0 33.0 2.0 38.0 50-51 15.090981012658228 2.0 2.0 33.0 2.0 37.5 52-53 14.747626582278482 2.0 2.0 33.0 2.0 38.0 54-55 14.599287974683545 2.0 2.0 33.0 2.0 38.0 56-57 14.359968354430379 2.0 2.0 32.5 2.0 38.0 58-59 14.007120253164556 2.0 2.0 32.0 2.0 38.0 60-61 13.564873417721518 2.0 2.0 31.5 2.0 38.0 62-63 12.96123417721519 2.0 2.0 30.5 2.0 37.0 64-65 12.761471518987342 2.0 2.0 30.0 2.0 36.5 66-67 12.264636075949367 2.0 2.0 28.5 2.0 36.0 68-69 11.99367088607595 2.0 2.0 28.0 2.0 35.0 70-71 11.725870253164556 2.0 2.0 28.0 2.0 35.0 72-73 11.147151898734178 2.0 2.0 26.0 2.0 34.0 74-75 10.738528481012658 2.0 2.0 25.5 2.0 34.0 76-77 10.030854430379748 2.0 2.0 22.0 2.0 33.5 78-79 9.251977848101266 2.0 2.0 15.5 2.0 32.5 80-81 8.955696202531644 2.0 2.0 10.0 2.0 32.0 82-83 8.640031645569621 2.0 2.0 4.5 2.0 32.0 84-85 8.145569620253164 2.0 2.0 2.0 2.0 31.0 86-87 7.403876582278482 2.0 2.0 2.0 2.0 30.0 88-89 7.112737341772152 2.0 2.0 2.0 2.0 29.5 90-91 6.557753164556962 2.0 2.0 2.0 2.0 29.0 92-93 5.952136075949367 2.0 2.0 2.0 2.0 26.0 94-95 5.6669303797468356 2.0 2.0 2.0 2.0 27.0 96-97 4.996439873417721 2.0 2.0 2.0 2.0 16.0 98-99 4.567246835443038 2.0 2.0 2.0 2.0 2.0 100-101 3.869462025316456 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 10.91578947368421 1101 2 10.410526315789475 1101 3 9.100000000000001 1101 4 10.910526315789475 1101 5 11.5 1101 6 12.042105263157893 1101 7 12.152631578947368 1101 8 11.284210526315789 1101 9 10.631578947368421 1101 10-11 13.344736842105263 1101 12-13 14.19736842105263 1101 14-15 13.268421052631577 1101 16-17 13.24736842105263 1101 18-19 15.023684210526316 1101 20-21 14.518421052631577 1101 22-23 16.557894736842105 1101 24-25 9.873684210526317 1101 26-27 13.899999999999999 1101 28-29 14.965789473684211 1101 30-31 17.742105263157896 1101 32-33 15.852631578947367 1101 34-35 10.607894736842105 1101 36-37 21.04736842105263 1101 38-39 20.655263157894737 1101 40-41 13.297368421052632 1101 42-43 18.376315789473683 1101 44-45 14.671052631578949 1101 46-47 12.528947368421054 1101 48-49 12.171052631578949 1101 50-51 20.078947368421055 1101 52-53 15.93421052631579 1101 54-55 18.789473684210527 1101 56-57 19.25 1101 58-59 14.284210526315789 1101 60-61 19.781578947368423 1101 62-63 20.565789473684212 1101 64-65 11.286842105263158 1101 66-67 20.289473684210527 1101 68-69 5.192105263157895 1101 70-71 6.128947368421052 1101 72-73 12.955263157894738 1101 74-75 10.189473684210526 1101 76-77 11.68157894736842 1101 78-79 21.031578947368423 1101 80-81 9.842105263157896 1101 82-83 12.352631578947369 1101 84-85 15.01578947368421 1101 86-87 13.35 1101 88-89 7.784210526315789 1101 90-91 -5.28421052631579 1101 92-93 -4.839473684210526 1101 94-95 -4.66578947368421 1101 96-97 -4.0 1101 98-99 -3.3157894736842106 1101 100-101 -2.681578947368421 1102 1 8.91578947368421 1102 2 8.410526315789475 1102 3 9.100000000000001 1102 4 10.910526315789475 1102 5 11.5 1102 6 12.042105263157893 1102 7 14.152631578947368 1102 8 11.284210526315789 1102 9 14.631578947368421 1102 10-11 9.844736842105263 1102 12-13 15.69736842105263 1102 14-15 15.768421052631577 1102 16-17 13.24736842105263 1102 18-19 17.023684210526316 1102 20-21 16.018421052631577 1102 22-23 14.057894736842105 1102 24-25 16.873684210526317 1102 26-27 15.399999999999999 1102 28-29 15.465789473684211 1102 30-31 10.742105263157896 1102 32-33 18.352631578947367 1102 34-35 20.107894736842105 1102 36-37 21.54736842105263 1102 38-39 20.155263157894737 1102 40-41 17.79736842105263 1102 42-43 19.376315789473683 1102 44-45 16.67105263157895 1102 46-47 17.028947368421054 1102 48-49 18.67105263157895 1102 50-51 20.078947368421055 1102 52-53 13.43421052631579 1102 54-55 2.7894736842105257 1102 56-57 13.75 1102 58-59 18.78421052631579 1102 60-61 19.281578947368423 1102 62-63 10.56578947368421 1102 64-65 13.286842105263158 1102 66-67 4.289473684210526 1102 68-69 18.692105263157895 1102 70-71 13.628947368421052 1102 72-73 17.955263157894738 1102 74-75 21.689473684210526 1102 76-77 20.681578947368422 1102 78-79 4.031578947368422 1102 80-81 18.842105263157897 1102 82-83 5.352631578947369 1102 84-85 13.01578947368421 1102 86-87 11.35 1102 88-89 19.78421052631579 1102 90-91 10.715789473684211 1102 92-93 -4.839473684210526 1102 94-95 -4.66578947368421 1102 96-97 -4.0 1102 98-99 -3.3157894736842106 1102 100-101 -2.681578947368421 1103 1 8.91578947368421 1103 2 11.410526315789475 1103 3 12.100000000000001 1103 4 12.910526315789475 1103 5 13.5 1103 6 14.042105263157893 1103 7 14.152631578947368 1103 8 13.284210526315789 1103 9 14.631578947368421 1103 10-11 14.844736842105263 1103 12-13 16.19736842105263 1103 14-15 16.768421052631577 1103 16-17 15.74736842105263 1103 18-19 17.023684210526316 1103 20-21 18.018421052631577 1103 22-23 17.057894736842105 1103 24-25 17.873684210526317 1103 26-27 19.9 1103 28-29 18.46578947368421 1103 30-31 19.742105263157896 1103 32-33 18.852631578947367 1103 34-35 13.607894736842105 1103 36-37 18.54736842105263 1103 38-39 20.155263157894737 1103 40-41 12.297368421052632 1103 42-43 19.376315789473683 1103 44-45 18.17105263157895 1103 46-47 19.528947368421054 1103 48-49 21.17105263157895 1103 50-51 20.578947368421055 1103 52-53 21.934210526315788 1103 54-55 15.289473684210526 1103 56-57 13.25 1103 58-59 17.28421052631579 1103 60-61 21.781578947368423 1103 62-63 15.06578947368421 1103 64-65 21.28684210526316 1103 66-67 21.789473684210527 1103 68-69 21.692105263157895 1103 70-71 22.128947368421052 1103 72-73 19.955263157894738 1103 74-75 7.189473684210526 1103 76-77 18.681578947368422 1103 78-79 16.531578947368423 1103 80-81 19.842105263157897 1103 82-83 15.852631578947369 1103 84-85 21.015789473684208 1103 86-87 22.85 1103 88-89 25.78421052631579 1103 90-91 -5.28421052631579 1103 92-93 -4.839473684210526 1103 94-95 -4.66578947368421 1103 96-97 -4.0 1103 98-99 -3.3157894736842106 1103 100-101 -2.681578947368421 1104 1 -20.08421052631579 1104 2 -20.589473684210525 1104 3 -19.9 1104 4 -22.089473684210525 1104 5 -21.5 1104 6 -20.957894736842107 1104 7 -20.847368421052632 1104 8 -21.71578947368421 1104 9 -22.36842105263158 1104 10-11 -21.655263157894737 1104 12-13 -20.80263157894737 1104 14-15 -21.731578947368423 1104 16-17 -21.25263157894737 1104 18-19 -20.976315789473684 1104 20-21 -20.481578947368423 1104 22-23 -20.442105263157895 1104 24-25 -20.126315789473683 1104 26-27 -19.1 1104 28-29 -18.53421052631579 1104 30-31 -18.257894736842104 1104 32-33 -17.647368421052633 1104 34-35 -17.892105263157895 1104 36-37 -16.95263157894737 1104 38-39 -16.844736842105263 1104 40-41 -16.70263157894737 1104 42-43 -16.623684210526317 1104 44-45 -15.328947368421051 1104 46-47 -14.471052631578946 1104 48-49 -14.328947368421051 1104 50-51 -13.421052631578947 1104 52-53 -13.56578947368421 1104 54-55 -13.210526315789474 1104 56-57 -12.75 1104 58-59 -12.715789473684211 1104 60-61 -12.218421052631578 1104 62-63 -11.43421052631579 1104 64-65 -10.713157894736842 1104 66-67 -10.210526315789474 1104 68-69 -10.307894736842105 1104 70-71 -10.371052631578948 1104 72-73 -10.044736842105262 1104 74-75 -9.310526315789474 1104 76-77 -8.81842105263158 1104 78-79 -8.468421052631578 1104 80-81 -8.157894736842104 1104 82-83 -8.147368421052631 1104 84-85 -7.48421052631579 1104 86-87 -6.15 1104 88-89 -6.215789473684211 1104 90-91 -5.28421052631579 1104 92-93 -4.839473684210526 1104 94-95 -4.66578947368421 1104 96-97 -4.0 1104 98-99 -3.3157894736842106 1104 100-101 -2.681578947368421 1105 1 9.41578947368421 1105 2 8.410526315789475 1105 3 10.600000000000001 1105 4 8.410526315789475 1105 5 11.5 1105 6 12.042105263157893 1105 7 13.652631578947368 1105 8 13.284210526315789 1105 9 0.13157894736842124 1105 10-11 4.844736842105263 1105 12-13 14.19736842105263 1105 14-15 11.768421052631577 1105 16-17 -3.002631578947369 1105 18-19 -0.9763157894736842 1105 20-21 11.268421052631577 1105 22-23 10.057894736842105 1105 24-25 3.623684210526317 1105 26-27 5.899999999999999 1105 28-29 13.215789473684211 1105 30-31 17.992105263157896 1105 32-33 17.352631578947367 1105 34-35 -0.1421052631578945 1105 36-37 -6.4526315789473685 1105 38-39 -8.594736842105263 1105 40-41 -4.7026315789473685 1105 42-43 -2.873684210526317 1105 44-45 1.1710526315789487 1105 46-47 -0.47105263157894584 1105 48-49 0.9210526315789487 1105 50-51 3.578947368421053 1105 52-53 -11.06578947368421 1105 54-55 -0.21052631578947434 1105 56-57 2.25 1105 58-59 -5.215789473684211 1105 60-61 -12.218421052631578 1105 62-63 -11.43421052631579 1105 64-65 -10.713157894736842 1105 66-67 -10.210526315789474 1105 68-69 -10.307894736842105 1105 70-71 -10.371052631578948 1105 72-73 -10.044736842105262 1105 74-75 -9.310526315789474 1105 76-77 -8.81842105263158 1105 78-79 -8.468421052631578 1105 80-81 -8.157894736842104 1105 82-83 -8.147368421052631 1105 84-85 -7.48421052631579 1105 86-87 -6.15 1105 88-89 -6.215789473684211 1105 90-91 -5.28421052631579 1105 92-93 -4.839473684210526 1105 94-95 -4.66578947368421 1105 96-97 -4.0 1105 98-99 -3.3157894736842106 1105 100-101 -2.681578947368421 1106 1 -6.08421052631579 1106 2 5.410526315789475 1106 3 4.100000000000001 1106 4 5.910526315789475 1106 5 -13.5 1106 6 -12.957894736842107 1106 7 -12.847368421052632 1106 8 -6.715789473684211 1106 9 -7.368421052631579 1106 10-11 -10.155263157894737 1106 12-13 -1.30263157894737 1106 14-15 -9.731578947368423 1106 16-17 2.747368421052631 1106 18-19 2.0236842105263158 1106 20-21 6.018421052631577 1106 22-23 0.05789473684210478 1106 24-25 -14.126315789473683 1106 26-27 -9.100000000000001 1106 28-29 -4.534210526315789 1106 30-31 5.742105263157896 1106 32-33 -7.647368421052633 1106 34-35 1.1078947368421055 1106 36-37 0.5473684210526315 1106 38-39 -4.844736842105263 1106 40-41 3.7973684210526315 1106 42-43 9.876315789473683 1106 44-45 0.17105263157894868 1106 46-47 -14.471052631578946 1106 48-49 -14.328947368421051 1106 50-51 -13.421052631578947 1106 52-53 -13.56578947368421 1106 54-55 -13.210526315789474 1106 56-57 -12.75 1106 58-59 -12.715789473684211 1106 60-61 -12.218421052631578 1106 62-63 -11.43421052631579 1106 64-65 -10.713157894736842 1106 66-67 -10.210526315789474 1106 68-69 -10.307894736842105 1106 70-71 -10.371052631578948 1106 72-73 -10.044736842105262 1106 74-75 -9.310526315789474 1106 76-77 -8.81842105263158 1106 78-79 -8.468421052631578 1106 80-81 -8.157894736842104 1106 82-83 -8.147368421052631 1106 84-85 -7.48421052631579 1106 86-87 -6.15 1106 88-89 -6.215789473684211 1106 90-91 -5.28421052631579 1106 92-93 -4.839473684210526 1106 94-95 -4.66578947368421 1106 96-97 -4.0 1106 98-99 -3.3157894736842106 1106 100-101 -2.681578947368421 1107 1 7.91578947368421 1107 2 8.410526315789475 1107 3 9.100000000000001 1107 4 10.910526315789475 1107 5 11.5 1107 6 7.042105263157893 1107 7 5.152631578947368 1107 8 8.284210526315789 1107 9 7.631578947368421 1107 10-11 6.844736842105263 1107 12-13 10.69736842105263 1107 14-15 13.768421052631577 1107 16-17 9.24736842105263 1107 18-19 14.023684210526316 1107 20-21 16.018421052631577 1107 22-23 18.057894736842105 1107 24-25 14.373684210526317 1107 26-27 10.899999999999999 1107 28-29 9.965789473684211 1107 30-31 16.742105263157896 1107 32-33 18.352631578947367 1107 34-35 19.107894736842105 1107 36-37 19.54736842105263 1107 38-39 21.155263157894737 1107 40-41 16.29736842105263 1107 42-43 18.376315789473683 1107 44-45 13.171052631578949 1107 46-47 14.028947368421054 1107 48-49 13.671052631578949 1107 50-51 14.578947368421053 1107 52-53 21.934210526315788 1107 54-55 24.789473684210527 1107 56-57 22.25 1107 58-59 23.28421052631579 1107 60-61 23.781578947368423 1107 62-63 22.565789473684212 1107 64-65 24.78684210526316 1107 66-67 27.289473684210527 1107 68-69 26.192105263157895 1107 70-71 13.128947368421052 1107 72-73 5.955263157894738 1107 74-75 6.689473684210526 1107 76-77 14.18157894736842 1107 78-79 24.531578947368423 1107 80-81 23.842105263157897 1107 82-83 25.852631578947367 1107 84-85 19.515789473684208 1107 86-87 5.85 1107 88-89 22.78421052631579 1107 90-91 15.715789473684211 1107 92-93 19.160526315789475 1107 94-95 25.83421052631579 1107 96-97 -4.0 1107 98-99 -3.3157894736842106 1107 100-101 -2.681578947368421 1108 1 -5.58421052631579 1108 2 3.9105263157894754 1108 3 6.100000000000001 1108 4 8.410526315789475 1108 5 -5.0 1108 6 -12.457894736842107 1108 7 -16.347368421052632 1108 8 -6.715789473684211 1108 9 -22.36842105263158 1108 10-11 -21.655263157894737 1108 12-13 -20.80263157894737 1108 14-15 -21.731578947368423 1108 16-17 -21.25263157894737 1108 18-19 -20.976315789473684 1108 20-21 -20.481578947368423 1108 22-23 -20.442105263157895 1108 24-25 -20.126315789473683 1108 26-27 -19.1 1108 28-29 -18.53421052631579 1108 30-31 -18.257894736842104 1108 32-33 -17.647368421052633 1108 34-35 -17.892105263157895 1108 36-37 -16.95263157894737 1108 38-39 -16.844736842105263 1108 40-41 -16.70263157894737 1108 42-43 -16.623684210526317 1108 44-45 -15.328947368421051 1108 46-47 -14.471052631578946 1108 48-49 -14.328947368421051 1108 50-51 -13.421052631578947 1108 52-53 -13.56578947368421 1108 54-55 -13.210526315789474 1108 56-57 -12.75 1108 58-59 -12.715789473684211 1108 60-61 -12.218421052631578 1108 62-63 -11.43421052631579 1108 64-65 -10.713157894736842 1108 66-67 -10.210526315789474 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5.910526315789475 2316 3 0.10000000000000142 2316 4 8.410526315789475 2316 5 0.0 2316 6 1.042105263157893 2316 7 4.152631578947368 2316 8 3.284210526315789 2316 9 0.13157894736842124 2316 10-11 0.844736842105263 2316 12-13 3.19736842105263 2316 14-15 9.518421052631577 2316 16-17 5.997368421052631 2316 18-19 10.523684210526316 2316 20-21 15.768421052631577 2316 22-23 2.3078947368421048 2316 24-25 6.873684210526317 2316 26-27 15.149999999999999 2316 28-29 6.965789473684211 2316 30-31 4.742105263157896 2316 32-33 11.102631578947367 2316 34-35 5.3578947368421055 2316 36-37 -0.9526315789473685 2316 38-39 -1.844736842105263 2316 40-41 -2.7026315789473685 2316 42-43 -5.123684210526317 2316 44-45 0.6710526315789487 2316 46-47 -1.2210526315789458 2316 48-49 -1.0789473684210513 2316 50-51 -13.421052631578947 2316 52-53 -13.56578947368421 2316 54-55 -13.210526315789474 2316 56-57 -12.75 2316 58-59 -12.715789473684211 2316 60-61 -12.218421052631578 2316 62-63 -11.43421052631579 2316 64-65 -10.713157894736842 2316 66-67 -10.210526315789474 2316 68-69 -10.307894736842105 2316 70-71 -10.371052631578948 2316 72-73 -10.044736842105262 2316 74-75 -9.310526315789474 2316 76-77 -8.81842105263158 2316 78-79 -8.468421052631578 2316 80-81 -8.157894736842104 2316 82-83 -8.147368421052631 2316 84-85 -7.48421052631579 2316 86-87 -6.15 2316 88-89 -6.215789473684211 2316 90-91 -5.28421052631579 2316 92-93 -4.839473684210526 2316 94-95 -4.66578947368421 2316 96-97 -4.0 2316 98-99 -3.3157894736842106 2316 100-101 -2.681578947368421 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 319.0 3 59.0 4 50.0 5 32.0 6 28.0 7 41.0 8 30.0 9 30.0 10 23.0 11 33.0 12 24.0 13 32.0 14 30.0 15 20.0 16 18.0 17 21.0 18 12.0 19 16.0 20 12.0 21 20.0 22 19.0 23 18.0 24 29.0 25 31.0 26 27.0 27 29.0 28 29.0 29 37.0 30 24.0 31 19.0 32 36.0 33 30.0 34 23.0 35 25.0 36 25.0 37 12.0 38 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.32299124900557 20.20684168655529 13.365155131264917 24.105011933174225 2 18.9873417721519 19.303797468354432 36.86708860759494 24.841772151898734 3 25.158227848101266 21.75632911392405 25.712025316455694 27.37341772151899 4 17.563291139240505 17.800632911392405 35.04746835443038 29.58860759493671 5 19.77848101265823 35.44303797468354 28.718354430379744 16.060126582278482 6 36.86708860759494 33.860759493670884 15.18987341772152 14.082278481012658 7 34.335443037974684 30.45886075949367 17.72151898734177 17.484177215189874 8 31.329113924050635 32.199367088607595 18.037974683544302 18.43354430379747 9 30.537974683544306 18.037974683544302 18.75 32.674050632911396 10-11 28.955696202531644 25.67246835443038 24.287974683544302 21.08386075949367 12-13 28.24367088607595 22.86392405063291 25.63291139240506 23.259493670886076 14-15 25.79113924050633 24.525316455696203 24.169303797468356 25.514240506329116 16-17 27.610759493670884 27.21518987341772 23.10126582278481 22.072784810126585 18-19 27.056962025316455 26.503164556962027 25.79113924050633 20.64873417721519 20-21 26.02848101265823 26.582278481012654 25.67246835443038 21.716772151898734 22-23 26.265822784810126 26.10759493670886 25.55379746835443 22.072784810126585 24-25 25.55379746835443 25.039556962025316 27.21518987341772 22.19145569620253 26-27 26.542721518987346 25.197784810126585 25.712025316455694 22.547468354430382 28-29 26.582278481012654 24.68354430379747 25.55379746835443 23.180379746835442 30-31 25.158227848101266 25.75158227848101 25.35601265822785 23.734177215189874 32-33 26.02848101265823 25.90981012658228 25.435126582278482 22.626582278481013 34-35 27.056962025316455 25.67246835443038 25.63291139240506 21.6376582278481 36-37 26.068037974683545 24.12974683544304 26.424050632911396 23.378164556962027 38-39 25.2373417721519 25.830696202531644 25.63291139240506 23.299050632911392 40-41 26.621835443037973 26.34493670886076 26.503164556962027 20.530063291139243 42-43 25.2373417721519 24.564873417721518 26.859177215189874 23.338607594936708 44-45 26.384493670886076 26.46360759493671 25.35601265822785 21.795886075949365 46-47 25.395569620253166 23.97151898734177 26.740506329113924 23.89240506329114 48-49 26.97784810126582 25.31645569620253 24.7626582278481 22.943037974683545 50-51 28.955696202531644 25.830696202531644 23.536392405063292 21.677215189873415 52-53 25.35601265822785 25.63291139240506 26.582278481012654 22.428797468354432 54-55 26.66139240506329 24.802215189873415 24.723101265822788 23.813291139240505 56-57 24.802215189873415 25.4746835443038 26.66139240506329 23.061708860759495 58-59 25.2373417721519 26.10759493670886 25.79113924050633 22.86392405063291 60-61 28.322784810126585 23.85284810126582 25.514240506329116 22.310126582278482 62-63 24.367088607594937 25.2373417721519 25.79113924050633 24.604430379746837 64-65 25.712025316455694 25.593354430379744 25.949367088607595 22.74525316455696 66-67 26.186708860759495 24.68354430379747 25.11867088607595 24.01107594936709 68-69 26.265822784810126 24.88132911392405 24.406645569620252 24.446202531645568 70-71 27.09651898734177 25.4746835443038 23.694620253164558 23.734177215189874 72-73 26.22626582278481 25.197784810126585 24.050632911392405 24.525316455696203 74-75 26.265822784810126 25.4746835443038 24.960443037974684 23.299050632911392 76-77 26.305379746835445 24.327531645569618 23.575949367088608 25.79113924050633 78-79 26.46360759493671 25.87025316455696 24.09018987341772 23.575949367088608 80-81 25.593354430379744 26.14715189873418 23.931962025316455 24.327531645569618 82-83 25.79113924050633 23.931962025316455 25.395569620253166 24.88132911392405 84-85 25.435126582278482 26.265822784810126 24.525316455696203 23.77373417721519 86-87 24.09018987341772 26.89873417721519 25.158227848101266 23.85284810126582 88-89 27.57120253164557 25.4746835443038 22.74525316455696 24.20886075949367 90-91 26.186708860759495 24.643987341772153 23.259493670886076 25.90981012658228 92-93 26.265822784810126 24.920886075949365 24.01107594936709 24.802215189873415 94-95 25.435126582278482 24.88132911392405 23.338607594936708 26.34493670886076 96-97 25.63291139240506 25.87025316455696 23.259493670886076 25.2373417721519 98-99 26.02848101265823 24.367088607594937 23.299050632911392 26.305379746835445 100-101 26.869806094182824 23.308270676691727 22.55639097744361 27.265532251681833 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 1.0 2 0.5 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.5 9 0.5 10 1.0 11 1.5 12 1.0 13 0.5 14 0.5 15 1.5 16 1.5 17 1.0 18 0.5 19 0.0 20 0.5 21 1.0 22 1.5 23 2.0 24 1.5 25 1.0 26 0.5 27 2.0 28 6.5 29 9.5 30 9.0 31 10.5 32 13.0 33 15.5 34 18.0 35 19.0 36 21.0 37 17.5 38 18.5 39 28.0 40 32.0 41 29.0 42 30.5 43 32.5 44 33.5 45 37.5 46 37.5 47 38.0 48 44.0 49 49.0 50 47.5 51 45.0 52 38.0 53 40.0 54 46.5 55 41.5 56 47.0 57 51.5 58 46.5 59 40.0 60 31.5 61 25.0 62 25.5 63 31.0 64 27.0 65 19.5 66 15.0 67 12.0 68 11.0 69 8.0 70 5.5 71 6.0 72 6.5 73 6.0 74 3.5 75 1.0 76 1.0 77 4.0 78 4.5 79 2.0 80 1.0 81 1.0 82 0.5 83 0.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.5537974683544303 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.03955696202531646 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1264.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.5253164556962 #Duplication Level Percentage of deduplicated Percentage of total 1 99.68203497615262 99.20886075949366 2 0.1589825119236884 0.31645569620253167 3 0.1589825119236884 0.4746835443037975 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3 0.23734177215189875 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3 0.23734177215189875 No Hit CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG 2 0.15822784810126583 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2 0.15822784810126583 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.07911392405063292 0.0 34-35 0.0 0.0 0.0 0.15822784810126583 0.0 36-37 0.0 0.0 0.0 0.15822784810126583 0.0 38-39 0.0 0.0 0.0 0.23734177215189872 0.0 40-41 0.0 0.0 0.0 0.23734177215189872 0.0 42-43 0.0 0.0 0.0 0.31645569620253167 0.0 44-45 0.0 0.0 0.0 0.31645569620253167 0.0 46-47 0.0 0.0 0.0 0.39556962025316456 0.0 48-49 0.0 0.0 0.0 0.39556962025316456 0.0 50-51 0.0 0.0 0.0 0.47468354430379744 0.0 52-53 0.0 0.0 0.0 0.47468354430379744 0.0 54-55 0.0 0.0 0.0 0.5142405063291139 0.0 56-57 0.0 0.0 0.0 0.5537974683544303 0.0 58-59 0.0 0.0 0.0 0.5537974683544303 0.0 60-61 0.0 0.0 0.0 0.5933544303797469 0.0 62-63 0.0 0.0 0.0 0.7120253164556962 0.0 64-65 0.0 0.0 0.0 0.8306962025316456 0.0 66-67 0.0 0.0 0.0 1.0680379746835442 0.0 68-69 0.0 0.0 0.0 1.1867088607594938 0.0 70-71 0.0 0.0 0.0 1.2658227848101267 0.0 72-73 0.0 0.0 0.0 1.2658227848101267 0.0 74-75 0.0 0.0 0.0 1.4240506329113924 0.0 76-77 0.0 0.0 0.0 1.4636075949367089 0.0 78-79 0.0 0.0 0.0 1.5822784810126582 0.0 80-81 0.0 0.0 0.0 1.6613924050632911 0.0 82-83 0.0 0.0 0.0 1.8987341772151898 0.0 84-85 0.0 0.0 0.0 2.2547468354430378 0.0 86-87 0.0 0.0 0.0 2.5316455696202533 0.0 88-89 0.0 0.0 0.0 2.768987341772152 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE