FastQCFastQC Report
Thu 26 May 2016
SRR938488_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938488_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1264
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG151.1867088607594938RNA PCR Primer, Index 33 (96% over 29bp)
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG131.0284810126582278RNA PCR Primer, Index 39 (96% over 30bp)
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG131.0284810126582278RNA PCR Primer, Index 23 (96% over 30bp)
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG80.6329113924050633RNA PCR Primer, Index 31 (96% over 29bp)
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG60.4746835443037975TruSeq Adapter, Index 10 (96% over 28bp)
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG50.39556962025316456RNA PCR Primer, Index 46 (96% over 28bp)
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG50.39556962025316456RNA PCR Primer, Index 17 (96% over 28bp)
CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT50.39556962025316456No Hit
GGATTTAAAAACTGGAACGGTGAAGGCGACAGCAGTTGGTTGGAGCAAAC50.39556962025316456No Hit
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGA40.31645569620253167RNA PCR Primer, Index 10 (96% over 28bp)
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGA30.23734177215189875RNA PCR Primer, Index 39 (96% over 30bp)
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG30.23734177215189875RNA PCR Primer, Index 26 (100% over 29bp)
TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC30.23734177215189875No Hit
GTGCTCGCCCACGTCGCTGTCGTAGCGCACGTACTCCTCCCGGTTGTAGA30.23734177215189875No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG30.23734177215189875TruSeq Adapter, Index 1 (96% over 28bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.23734177215189875No Hit
GAACTGCTACGTAACACAGTTCCACCCGCCTCACATTGAAATCCAAATGC20.15822784810126583No Hit
GATCCAGGACAAGGAGGGCATCCCCCCTGACCAGCAGAGGCTGATCTTTG20.15822784810126583No Hit
CCTCTGTCCTGGACAGGGAGATGACGACATTGGGCTGTTCAAGCCGCCGC20.15822784810126583No Hit
GAGCAGTGTTTGCTATCATAGCAGGATTTGTTCACTGATTCCCATTATTT20.15822784810126583No Hit
CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG20.15822784810126583No Hit
GTTCAAGGGCAAGAAAGGTGTTTTGTTTGGAGTCCCTGGGGCATTTACAC20.15822784810126583No Hit
GTTCTGACCCAGTGACCGCATGACGGTCCCCAGTTCCTTGGTTGTGATGG20.15822784810126583No Hit
CTCAGAGACCACGGCTGGAGTAATAAGCTTATAGTTCGGAACCTCCTTAC20.15822784810126583No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT20.15822784810126583No Hit
TCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAA20.15822784810126583TruSeq Adapter, Index 3 (96% over 28bp)
TCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAA20.15822784810126583RNA PCR Primer, Index 38 (96% over 28bp)
ACTCAGACTACCCAGATGCTTACACCACATGAAACACTGTCTCTTCTATA20.15822784810126583No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.15822784810126583No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.15822784810126583No Hit
GCCCTGGCGTCTCCAAATGCCTCAGCGGGAAGGTCATGGCCTTGGGTTTT20.15822784810126583No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.15822784810126583No Hit
CTCTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCT20.15822784810126583No Hit
CTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGA20.15822784810126583RNA PCR Primer, Index 31 (96% over 29bp)
ACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCACTAACAGCTGT20.15822784810126583No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.15822784810126583No Hit
CTCGTAGTTGTGTCTGCACACCGTGTCCAGCTCGGCCCGCGTTCGCTCCA20.15822784810126583No Hit
CTTTATAATTTGAGAGGCCTTTGCTTCAAAACGAGAAGTAATATCAATCT20.15822784810126583No Hit
GTAGTTGTGTCTGCACACCGTGTCCAGCTCGGCCCGCGTTCGCTCCAGGA20.15822784810126583No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers