FastQCFastQC Report
Thu 26 May 2016
SRR938487_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938487_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2267068
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143270.6319616350281508No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105640.46597631831069913No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82010.3617447734254111No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40210.17736565466937915No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24930.10996582369827461No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23770.10484908260360959No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62900.050.360561
GTATCAA115450.042.886811
TATCAAC149050.033.0260122
ATCAACG148900.032.9315153
TCAACGC150100.032.7632984
CAACGCA152100.032.207415
AACGCAG157550.031.249556
ACGCAGA178350.027.3888917
CGCAGAG180650.026.9349698
GCAGAGT189050.024.8070879
GTACATG139650.024.249681
TACATGG138500.023.9691932
ACATGGG140350.022.8738423
GAGTACT105550.022.82263612-13
GTGTAGC17600.022.4301621
GTACTTT109900.021.74395214-15
AGTACTT110550.021.31763612-13
CATGGGA86800.021.2598274
GTAGCCC15450.021.2406793
AGGACCG4300.021.015175
Produced by FastQC (version 0.11.5)