FastQCFastQC Report
Thu 26 May 2016
SRR938487_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938487_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2267068
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141590.6245511824082912No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113140.49905869607792974No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89470.3946507118445498No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42930.1893635303396281No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26520.11697928778492749No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25340.11177432701621655No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64200.051.382681
GTATCAA114700.040.529171
TATCAAC149650.030.8732132
ATCAACG149150.030.8308543
TCAACGC151500.030.415334
CAACGCA154100.029.8405065
AACGCAG159450.028.9139776
ACGCAGA182600.025.0662487
CGCAGAG184700.024.7812528
TACATGG132700.023.9275282
GTACATG135600.023.87151
GCAGAGT198850.022.6119219
ACATGGG136650.022.596293
GAGTACT112000.021.7688512-13
GTGGTAT32200.021.6997011
TGGTATC32500.021.4993952
GTACTTT115450.020.99495114-15
AGTACTT117050.020.82965712-13
CATGGGG55300.020.7885254
CAGAGTA194400.019.7101710-11
Produced by FastQC (version 0.11.5)