##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938487_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2267068 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.780982308426566 31.0 31.0 34.0 30.0 34.0 2 31.98991957894514 31.0 31.0 34.0 30.0 34.0 3 32.04432597522439 33.0 31.0 34.0 30.0 34.0 4 35.733264727833486 37.0 35.0 37.0 33.0 37.0 5 35.635670831223415 37.0 35.0 37.0 33.0 37.0 6 35.64055643677208 37.0 35.0 37.0 33.0 37.0 7 35.580355772301495 37.0 35.0 37.0 33.0 37.0 8 35.562237215645936 37.0 35.0 37.0 33.0 37.0 9 37.12475232326511 39.0 37.0 39.0 33.0 39.0 10-11 37.03419129906999 39.0 37.0 39.0 33.0 39.0 12-13 36.914950941039265 39.0 37.0 39.0 33.0 39.0 14-15 37.911950589924956 40.0 37.0 41.0 32.5 41.0 16-17 37.834264344960104 40.0 37.0 41.0 32.5 41.0 18-19 38.10692290659125 40.0 37.0 41.0 33.0 41.0 20-21 38.09875310312704 40.0 37.0 41.0 33.0 41.0 22-23 37.97972336956809 40.0 37.0 41.0 33.0 41.0 24-25 37.85625442201116 40.0 37.0 41.0 32.0 41.0 26-27 37.66388304188494 40.0 37.0 41.0 32.0 41.0 28-29 37.538398936423604 39.5 36.5 41.0 32.0 41.0 30-31 37.26674365303555 39.0 36.0 41.0 31.5 41.0 32-33 37.03987198443099 39.0 35.5 41.0 31.0 41.0 34-35 36.91398846439542 39.0 35.0 41.0 31.0 41.0 36-37 36.86121280879091 39.0 35.0 41.0 31.0 41.0 38-39 36.6958018021515 39.0 35.0 41.0 30.0 41.0 40-41 36.51573464051366 39.0 35.0 40.5 30.0 41.0 42-43 36.29356949151945 39.0 35.0 40.0 30.0 41.0 44-45 35.95226014393922 38.5 35.0 40.0 29.5 41.0 46-47 35.52723716271413 38.0 35.0 40.0 27.5 41.0 48-49 35.146477961843225 38.0 34.0 40.0 26.5 41.0 50-51 34.78319574004838 38.0 34.0 40.0 25.5 41.0 52-53 34.32108432565764 38.0 33.0 40.0 24.0 41.0 54-55 34.00538382615784 37.5 33.0 40.0 23.0 41.0 56-57 33.694688690414225 37.0 33.0 40.0 23.0 41.0 58-59 33.242210643880114 37.0 32.0 39.5 22.0 41.0 60-61 32.87161809879545 36.0 31.5 39.0 20.5 40.5 62-63 32.397726270230976 36.0 31.0 39.0 18.5 40.0 64-65 31.889932503127387 35.0 30.5 38.5 17.5 40.0 66-67 31.41646479064589 35.0 30.0 38.0 13.5 40.0 68-69 30.93583562557453 34.5 29.0 38.0 10.0 40.0 70-71 30.394265853516522 34.0 29.0 37.0 8.0 39.0 72-73 29.901837527590704 34.0 28.5 36.5 7.0 39.0 74-75 29.308066189457044 34.0 28.0 36.0 4.0 39.0 76-77 28.07301346937983 31.5 26.5 34.5 2.0 37.0 78-79 28.77174129757025 33.0 28.0 35.0 2.0 37.0 80-81 28.392395596426752 33.0 27.0 35.0 2.0 37.0 82-83 27.94322534657099 33.0 26.0 35.0 2.0 36.5 84-85 27.38288639776134 33.0 26.0 35.0 2.0 36.0 86-87 26.75637298925308 32.0 25.0 35.0 2.0 35.5 88-89 26.00494228668924 32.0 23.5 34.0 2.0 35.0 90-91 25.38468409416921 31.0 21.0 34.0 2.0 35.0 92-93 24.552851745073372 31.0 18.5 34.0 2.0 35.0 94-95 23.73306071983725 31.0 8.5 34.0 2.0 35.0 96-97 22.655192742343857 30.0 2.0 34.0 2.0 35.0 98-99 21.31329430789019 29.5 2.0 34.0 2.0 35.0 100-101 17.433172494164268 23.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.19485718982138067 1101 2 0.2526652733262935 1101 3 0.24379284194329642 1101 4 0.13804261951401742 1101 5 -0.006503354339471912 1101 6 0.12950655388465293 1101 7 0.1576439763460229 1101 8 0.2652283357501801 1101 9 0.2798364084918816 1101 10-11 0.21092539168515856 1101 12-13 0.2891369028175106 1101 14-15 0.41655975499413245 1101 16-17 0.3046148272008935 1101 18-19 0.3567730111688121 1101 20-21 0.13667799220985444 1101 22-23 0.3131292888645092 1101 24-25 0.303084886570268 1101 26-27 0.18672439247347938 1101 28-29 0.26556669695960267 1101 30-31 0.4068611350282936 1101 32-33 0.2803707628769061 1101 34-35 0.40978124342943545 1101 36-37 0.4097481365959226 1101 38-39 0.47009233725157173 1101 40-41 0.5701399414908082 1101 42-43 0.6229374881194758 1101 44-45 0.569828533640127 1101 46-47 0.5134994098428436 1101 48-49 0.48828645338443977 1101 50-51 0.552215126951765 1101 52-53 0.3991472473047395 1101 54-55 0.403836263543667 1101 56-57 0.3322966390019815 1101 58-59 0.643851916898825 1101 60-61 0.6057955430345032 1101 62-63 0.7395005902225478 1101 64-65 0.6520154642656628 1101 66-67 0.504377048639487 1101 68-69 0.5639126004045458 1101 70-71 0.5994887074784891 1101 72-73 0.6950457931096778 1101 74-75 0.49260447148082065 1101 76-77 0.47745398872211453 1101 78-79 0.7093918807640769 1101 80-81 0.6643493882253786 1101 82-83 0.6708008428129588 1101 84-85 0.918137724360232 1101 86-87 0.8378603485202483 1101 88-89 0.8986837484178771 1101 90-91 1.1080265795130764 1101 92-93 1.3689349271002271 1101 94-95 0.9729546227451316 1101 96-97 0.8562303575795589 1101 98-99 0.9567782534769371 1101 100-101 1.1573056669875967 1102 1 0.2129368723582452 1102 2 0.23539055456139124 1102 3 0.16388231766100603 1102 4 0.11890569204625479 1102 5 -0.009605244626136766 1102 6 0.025710843692166918 1102 7 0.17405930401486813 1102 8 0.1559859220548674 1102 9 0.09479845652901986 1102 10-11 0.2533323361445241 1102 12-13 0.2708749004245803 1102 14-15 0.27300885563516175 1102 16-17 0.2844581465855711 1102 18-19 0.25716318266861293 1102 20-21 0.28764163615891647 1102 22-23 0.27712608115628967 1102 24-25 0.307783155968238 1102 26-27 0.242992668257898 1102 28-29 0.36841179772958554 1102 30-31 0.2675720353029476 1102 32-33 0.3114443266612952 1102 34-35 0.3209355278409376 1102 36-37 0.47171130915296544 1102 38-39 0.4653239897538981 1102 40-41 0.2979485432505129 1102 42-43 0.3221321505976533 1102 44-45 0.3920989098987917 1102 46-47 0.4664420826869744 1102 48-49 0.3058216524459496 1102 50-51 0.4362716117688734 1102 52-53 0.30889307870898364 1102 54-55 0.2359722346789681 1102 56-57 0.20928528027639715 1102 58-59 0.43109480619084906 1102 60-61 0.29148650608979665 1102 62-63 0.1813349498885799 1102 64-65 0.32733405098661095 1102 66-67 0.15490574118303968 1102 68-69 0.14137938455325383 1102 70-71 0.16021138087389986 1102 72-73 0.367384191843783 1102 74-75 0.32786137078653255 1102 76-77 0.3897427207710287 1102 78-79 0.4426629535990507 1102 80-81 0.5693809490655504 1102 82-83 0.5086919778866275 1102 84-85 0.4237132547696909 1102 86-87 0.38758648330659895 1102 88-89 0.4566710399880911 1102 90-91 0.5440333612043773 1102 92-93 0.792381260590691 1102 94-95 0.8775221497684136 1102 96-97 0.8693830825197466 1102 98-99 1.1116167491524216 1102 100-101 1.3002322872750156 1103 1 0.17702016977840174 1103 2 0.27056387640250534 1103 3 0.2515904488450076 1103 4 0.21570250101723332 1103 5 0.2112312724213865 1103 6 0.18173861713686534 1103 7 0.14247157142747113 1103 8 0.1818352576831188 1103 9 0.27356918592344925 1103 10-11 0.2612497596318377 1103 12-13 0.34511151406312734 1103 14-15 0.5267455886905239 1103 16-17 0.5350344098701427 1103 18-19 0.4045559300575263 1103 20-21 0.34469996356547483 1103 22-23 0.3208262488686273 1103 24-25 0.39636410955783674 1103 26-27 0.4691639445901927 1103 28-29 0.47835767679162444 1103 30-31 0.4364782080510565 1103 32-33 0.46135617413207797 1103 34-35 0.34814581866276484 1103 36-37 0.3804840170770447 1103 38-39 0.37267900847722046 1103 40-41 0.3932224572404408 1103 42-43 0.5502989216989604 1103 44-45 0.5968453320209548 1103 46-47 0.6181933237846948 1103 48-49 0.7878681483333381 1103 50-51 0.8824040489913187 1103 52-53 0.8488959517631685 1103 54-55 0.8095331077349215 1103 56-57 0.8710757967299187 1103 58-59 0.8622598208513566 1103 60-61 0.9600841119367232 1103 62-63 1.1828919954370747 1103 64-65 1.0617533503315322 1103 66-67 1.0037278991287515 1103 68-69 0.9065828866829264 1103 70-71 0.8736140905341472 1103 72-73 0.8544907001928266 1103 74-75 0.949209573497253 1103 76-77 0.9982258539159368 1103 78-79 1.2002040598659214 1103 80-81 1.173218723735257 1103 82-83 1.1270504636636858 1103 84-85 1.182862962925551 1103 86-87 1.251116739828035 1103 88-89 1.2835315188363303 1103 90-91 1.4388419021070646 1103 92-93 1.678081294344672 1103 94-95 1.311103151829787 1103 96-97 1.5813694249366925 1103 98-99 1.7880069636708633 1103 100-101 1.9915592990078324 1104 1 0.2154464785266974 1104 2 0.22056185958222585 1104 3 0.21515743080791339 1104 4 0.17417533108667982 1104 5 0.14895270248142367 1104 6 0.13121894977775383 1104 7 0.18352814995909483 1104 8 0.08199172932326348 1104 9 0.22000411993980862 1104 10-11 0.2599295826894661 1104 12-13 0.335082034873345 1104 14-15 0.36776426780770066 1104 16-17 0.42389206605606944 1104 18-19 0.3230768108701696 1104 20-21 0.32102627500286474 1104 22-23 0.3430801799874885 1104 24-25 0.37667826293029805 1104 26-27 0.311262883070448 1104 28-29 0.2886782503753125 1104 30-31 0.2635232638161398 1104 32-33 0.40000282049609126 1104 34-35 0.43481656939049884 1104 36-37 0.4292217396835767 1104 38-39 0.3562839402750768 1104 40-41 0.37110844302858226 1104 42-43 0.37653556866801097 1104 44-45 0.5351999596150918 1104 46-47 0.581250638455721 1104 48-49 0.647317203453035 1104 50-51 0.6117438260654495 1104 52-53 0.7861480509369443 1104 54-55 0.6412378586912268 1104 56-57 0.7555098937658684 1104 58-59 0.9671143072680692 1104 60-61 0.9418953341970422 1104 62-63 0.8474964633662694 1104 64-65 0.8287118676997203 1104 66-67 0.7794520216438592 1104 68-69 0.7768446195349128 1104 70-71 0.9584394507046348 1104 72-73 0.9346210708171689 1104 74-75 0.9938497895659317 1104 76-77 0.9093887020695384 1104 78-79 1.1775639916973937 1104 80-81 1.1677884351282763 1104 82-83 1.22129647540158 1104 84-85 1.3145567054044882 1104 86-87 1.3345357880233166 1104 88-89 1.4655856839063475 1104 90-91 1.358138838557057 1104 92-93 1.5124730381547131 1104 94-95 1.4214261624046465 1104 96-97 1.6675162225622202 1104 98-99 1.809770135187982 1104 100-101 1.7785645897997 1105 1 0.19990559563354182 1105 2 0.27122054600948786 1105 3 0.24972580119236198 1105 4 0.16648168361059135 1105 5 0.15046483117861698 1105 6 0.14421533966945077 1105 7 0.16348254859104827 1105 8 0.24126875763410993 1105 9 0.28858192727403065 1105 10-11 0.2697497106266411 1105 12-13 0.34473907458454534 1105 14-15 0.3983274763706177 1105 16-17 0.3119921673924395 1105 18-19 0.35039171365059474 1105 20-21 0.46768217467985096 1105 22-23 0.3714424891900947 1105 24-25 0.3043985112044112 1105 26-27 0.17805226175180877 1105 28-29 0.29937477960452696 1105 30-31 0.31732575289081666 1105 32-33 0.4883776384073002 1105 34-35 0.5063774662174012 1105 36-37 0.5256876336604037 1105 38-39 0.515314752866125 1105 40-41 0.4792866050401088 1105 42-43 0.7243322942364472 1105 44-45 0.6909500071165198 1105 46-47 0.5488681253136534 1105 48-49 0.6003240436582402 1105 50-51 0.6030866913514075 1105 52-53 0.45841276054489555 1105 54-55 0.5001008329137875 1105 56-57 0.5700540384235353 1105 58-59 0.6029280066790506 1105 60-61 0.618543633646027 1105 62-63 0.6137664931338236 1105 64-65 0.7391693064228839 1105 66-67 0.6150321723817136 1105 68-69 0.5719337638759079 1105 70-71 0.5903738670704577 1105 72-73 0.6403465759156575 1105 74-75 0.7380498655682111 1105 76-77 0.6210978033425754 1105 78-79 0.9498009921407409 1105 80-81 0.7059946229805796 1105 82-83 1.1016253436009578 1105 84-85 0.9199243664343193 1105 86-87 0.9115854745139096 1105 88-89 1.2175680767114656 1105 90-91 1.220520084994451 1105 92-93 1.4379987205918532 1105 94-95 1.5724086502126156 1105 96-97 1.6497451477633085 1105 98-99 1.6200502352576507 1105 100-101 1.5774886825224819 1106 1 0.053359323410930415 1106 2 0.10751220974827191 1106 3 0.13935700537103202 1106 4 0.11972226169130096 1106 5 0.05283758549430928 1106 6 -0.02828132254683169 1106 7 0.00992985845449823 1106 8 0.16820877121947575 1106 9 -0.12488573721429219 1106 10-11 0.09704634005066737 1106 12-13 0.1209184248293127 1106 14-15 0.1881407947132132 1106 16-17 0.25609483059249527 1106 18-19 0.14042784369532058 1106 20-21 0.30238780557952794 1106 22-23 0.32123958707499867 1106 24-25 0.24265419714824077 1106 26-27 0.21218754154006803 1106 28-29 0.3034864021187502 1106 30-31 0.30763944600209925 1106 32-33 0.12572275353155504 1106 34-35 0.23386060162602007 1106 36-37 0.32320684055614635 1106 38-39 0.28802485756712315 1106 40-41 0.28331800363004334 1106 42-43 0.32020193328734337 1106 44-45 0.20601043856898116 1106 46-47 -0.4637141481269609 1106 48-49 -0.11358953702242758 1106 50-51 0.3884642708140831 1106 52-53 0.2424312179032455 1106 54-55 0.3962579898247611 1106 56-57 0.3199533193361006 1106 58-59 0.18153779048436292 1106 60-61 0.22837106437236798 1106 62-63 0.04333720730242874 1106 64-65 0.22964666805124878 1106 66-67 0.21371023435908754 1106 68-69 0.19513428855208304 1106 70-71 0.3487628301323191 1106 72-73 0.5669689144723264 1106 74-75 0.6078842060924217 1106 76-77 0.49543815187746887 1106 78-79 0.6605671013389731 1106 80-81 0.42785681350361315 1106 82-83 0.84234776882289 1106 84-85 0.9286351035575002 1106 86-87 1.3461997555435907 1106 88-89 1.256307448505435 1106 90-91 1.1407977066186028 1106 92-93 1.3828359889965114 1106 94-95 1.561195453669857 1106 96-97 1.766915702655215 1106 98-99 1.690118220641672 1106 100-101 1.5888842860172048 1107 1 0.06323826526474363 1107 2 0.14240923652518234 1107 3 0.08663040507280328 1107 4 0.061481794227610465 1107 5 -0.021411897212352926 1107 6 0.0230720021326718 1107 7 0.10971991059631847 1107 8 0.1187371764744043 1107 9 0.09404032421215192 1107 10-11 0.08004538992577181 1107 12-13 0.18911696445423587 1107 14-15 0.2812061737445717 1107 16-17 0.15575170811056438 1107 18-19 0.2931686986730426 1107 20-21 0.25067895572452414 1107 22-23 0.2434129655069981 1107 24-25 0.2786391253501108 1107 26-27 0.30263993680325285 1107 28-29 0.2507339862592417 1107 30-31 0.07470086627326111 1107 32-33 0.11917065183619968 1107 34-35 0.18945278534751253 1107 36-37 0.2789129494719944 1107 38-39 0.2848234473640332 1107 40-41 0.2159508934408052 1107 42-43 0.28601483444573006 1107 44-45 0.21889352606520873 1107 46-47 0.18035913214978905 1107 48-49 0.2571090152296662 1107 50-51 0.43380116669487734 1107 52-53 0.35813179330965994 1107 54-55 0.21679045261247154 1107 56-57 0.21441257031442262 1107 58-59 0.22213416362247784 1107 60-61 0.15622891715744913 1107 62-63 0.40181349430636715 1107 64-65 0.24828746752551822 1107 66-67 0.29738239693427104 1107 68-69 0.44394102035253624 1107 70-71 0.6329127489536042 1107 72-73 0.5273322067645303 1107 74-75 0.4996920305645638 1107 76-77 0.5328555631266525 1107 78-79 0.9961337613272647 1107 80-81 0.8806171423938665 1107 82-83 0.887791855403794 1107 84-85 0.9101643500630026 1107 86-87 1.0100575769010263 1107 88-89 1.1195680324646595 1107 90-91 1.0609608385176017 1107 92-93 1.2237708827121594 1107 94-95 1.223559620323897 1107 96-97 1.3639751979834394 1107 98-99 1.5055784868450068 1107 100-101 1.5315375420609882 1108 1 0.14585701263759177 1108 2 0.13975405927983786 1108 3 0.1556299847281224 1108 4 0.1671564474432472 1108 5 0.07293446680614579 1108 6 0.13695038240449975 1108 7 0.04451043882946948 1108 8 0.052469837256246876 1108 9 0.1085211985290897 1108 10-11 0.1389917259212865 1108 12-13 0.20200403184949778 1108 14-15 0.30303957713535823 1108 16-17 0.2530965308652213 1108 18-19 0.2858336839609734 1108 20-21 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-1.3204994742141807 2316 90-91 -1.4032779385454113 2316 92-93 -1.4969449028511015 2316 94-95 -1.353564438435356 2316 96-97 -1.7824745340649635 2316 98-99 -1.8055264005205167 2316 100-101 -2.2308610540167937 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 15.0 4 38.0 5 128.0 6 248.0 7 555.0 8 1149.0 9 2041.0 10 3268.0 11 4936.0 12 7156.0 13 10173.0 14 13035.0 15 17648.0 16 21549.0 17 27304.0 18 25801.0 19 20326.0 20 19824.0 21 21506.0 22 23483.0 23 26448.0 24 30552.0 25 36127.0 26 42538.0 27 50547.0 28 60474.0 29 72077.0 30 85037.0 31 100498.0 32 120636.0 33 148170.0 34 187695.0 35 241877.0 36 308994.0 37 350838.0 38 176874.0 39 7500.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.42586647540557 20.13600887140955 15.312091706520082 22.126032946664797 2 15.628581604196794 21.335316928804605 40.06992306432842 22.966178402670177 3 18.988931959694195 26.05493086224145 30.467414298997646 24.488722879066707 4 12.872000310533252 17.44499944421605 37.31515772795523 32.36784251729547 5 13.02931998586053 38.65759751667809 34.06372559186971 14.249356905591668 6 30.93696351410721 37.119265941736195 17.521838780310077 14.42193176384652 7 27.75779112051337 33.8939105487793 20.698276364008493 17.650021966698837 8 26.3570391360118 35.8405658762772 19.55358198342529 18.24881300428571 9 26.812561422948058 16.78599847909282 20.25528127078676 36.146158827172364 10-11 24.884344007325762 27.646347617274824 27.011739392025298 20.45756898337412 12-13 25.65580300193907 24.859752773185452 27.91819654284741 21.566247682028063 14-15 22.681344361968854 26.038963101239133 26.70312491729406 24.57656761949796 16-17 21.37860443533233 29.341157830290047 27.335659980203502 21.944577754174112 18-19 21.2827317045629 27.50954095774807 30.38660507757156 20.821122260117473 20-21 22.41496505618711 26.55462915095621 30.43808125737737 20.592324535479307 22-23 21.828745322151782 26.60008433800839 30.312610825965518 21.25855951387431 24-25 21.41996313938131 26.83769329106743 30.579800416247966 21.162543153303297 26-27 21.303090768057086 27.153554293941557 30.445952195950156 21.0974027420512 28-29 21.233999700935176 27.264566949278517 30.517624754804334 20.983808594981976 30-31 21.583220299732027 27.0437467606224 30.37606554846109 20.99696739118448 32-33 21.226087389950937 27.07576386478152 30.313132841388637 21.385015903878905 34-35 21.514145818104335 27.241138653325 30.254064901669018 20.99065062690165 36-37 21.61823807551004 27.06600786311075 30.169712949248918 21.14604111213029 38-39 21.36924568922261 27.24031507344516 30.199604007739865 21.190835229592363 40-41 21.4905905980527 27.147832791731602 30.23445539802765 21.127121212188047 42-43 21.441676975660044 27.20248730334157 29.915989331432463 21.439846389565925 44-45 21.43258576903073 27.358568389142224 29.882640094967826 21.32620574685922 46-47 21.514198074340953 27.39487743640685 29.43277396178677 21.65815052746543 48-49 21.486210382749878 27.560620149020675 29.33178448992267 21.621384978306782 50-51 21.645566242202715 27.61006512632796 29.078555862439188 21.665812769030136 52-53 21.682565322257645 27.754901926188364 28.904271949496003 21.65826080205799 54-55 21.446359014578764 27.649914723246997 29.001616872821074 21.902109389353164 56-57 21.547699495559904 27.979024008102094 28.629379445168823 21.843897051169176 58-59 21.601841728853252 27.71640005188466 28.872135203651695 21.8096230156104 60-61 21.829649573810755 27.631217943175944 28.94205202490618 21.597080458107122 62-63 21.632144037984045 27.654497117471344 28.917352807082437 21.796006037462178 64-65 21.4344278838741 27.61924173131377 28.957822272132606 21.988508112679522 66-67 21.543686866347556 27.86337126590872 28.553405351850113 22.039536515893612 68-69 21.518106955580322 27.689330471019957 28.667405185869487 22.12515738753023 70-71 21.514194999175146 27.620016355941996 28.640013127099078 22.225775517783777 72-73 21.499139306615263 27.76842771554001 28.41104201793727 22.321390959907458 74-75 21.344585011992816 27.904421910595566 28.297660049974287 22.453333027437328 76-77 21.269666216308355 27.742557448266712 28.594487093516317 22.393289241908608 78-79 21.348840862290267 27.81615022703577 28.474732646688576 22.36027626398539 80-81 21.207055958762513 27.943312133807403 28.369920513359588 22.4797113940705 82-83 21.38085915006334 27.906293311941287 28.313461412024992 22.399386125970373 84-85 21.395170421395324 27.72901543397849 28.162152396888985 22.713661747737206 86-87 21.171007928019417 27.64342859764513 28.413974824353 22.77158864998245 88-89 21.27976348141791 27.631785738250887 28.241182204422405 22.847268575908796 90-91 21.252827018665606 27.62598377602335 28.385385184877975 22.73580402043307 92-93 21.325704614513068 27.57119605669124 28.160954025174245 22.94214530362145 94-95 21.023452022253235 27.60824493912746 28.212580251908598 23.15572278671071 96-97 21.088829513788532 27.40527624498333 28.14693792899552 23.358956312232618 98-99 20.90704473989077 27.409014177745906 28.040094886081533 23.64384619628179 100-101 21.093039830222654 27.44660984628321 28.239623228756273 23.22072709473787 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3228.0 1 2132.5 2 1426.0 3 1795.0 4 1446.0 5 1321.0 6 2383.5 7 4979.5 8 7196.5 9 7269.0 10 5650.5 11 4032.0 12 3708.0 13 3770.5 14 3786.0 15 3758.0 16 3807.5 17 4089.5 18 4373.0 19 4611.0 20 5116.5 21 5755.5 22 6499.5 23 7738.0 24 9384.5 25 11514.5 26 14646.5 27 18213.5 28 20887.0 29 23946.5 30 28068.5 31 32092.0 32 36677.5 33 41650.0 34 46735.5 35 51959.5 36 58674.0 37 64686.0 38 67107.0 39 68926.0 40 70632.0 41 72765.5 42 74631.0 43 76734.0 44 79915.5 45 82536.5 46 83256.5 47 82913.5 48 83815.0 49 83288.0 50 80902.0 51 77459.5 52 73843.0 53 71237.5 54 68300.0 55 63291.5 56 57012.5 57 51022.0 58 45855.0 59 40965.5 60 34953.0 61 28524.5 62 22608.5 63 17012.0 64 12431.0 65 9371.0 66 6912.5 67 5324.0 68 4060.0 69 2867.5 70 2230.5 71 1662.0 72 1258.5 73 1134.0 74 951.5 75 708.0 76 494.0 77 297.0 78 183.0 79 125.5 80 65.0 81 33.0 82 23.5 83 14.0 84 8.0 85 5.0 86 2.0 87 1.0 88 1.0 89 1.0 90 1.0 91 1.5 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.4109837022974173E-4 2 0.05068220273939732 3 0.0 4 0.0 5 0.047241635451605334 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.006616475553446125 26-27 0.0018746680734764022 28-29 4.4109837022974166E-5 30-31 0.0018967229919878892 32-33 4.8520820725271585E-4 34-35 0.01257130355154764 36-37 0.02359876280729118 38-39 0.004609477968900801 40-41 0.0011909655996203026 42-43 0.0015217893772926088 44-45 0.008601418219479963 46-47 0.0 48-49 0.0 50-51 5.513729627871771E-4 52-53 0.0 54-55 0.0016541188883615313 56-57 0.0 58-59 0.022143138185533033 60-61 0.0 62-63 0.009770328900588779 64-65 0.014931179832276754 66-67 0.006660585390469099 68-69 0.01632063969850044 70-71 8.821967404594833E-5 72-73 2.2054918511487083E-5 74-75 0.02216519310404452 76-77 0.04827821662164522 78-79 0.030215238360737307 80-81 0.02587041941397435 82-83 0.0355084188034942 84-85 0.015372278202506497 86-87 0.010343756781887441 88-89 0.08275005425509953 90-91 0.024083971014543893 92-93 0.024039861177520924 94-95 0.026951110421037216 96-97 0.03850788772105645 98-99 0.05262303556840818 100-101 0.01475474048418486 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2267068.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.13511064123247 #Duplication Level Percentage of deduplicated Percentage of total 1 72.00146822087908 36.09801575584956 2 14.776240703736432 14.816169250866174 3 5.502627304040387 8.276244861165948 4 2.5502255587174814 5.114233621855993 5 1.4100201442892506 3.5345757970154073 6 0.8659876735847347 2.6049832697468513 7 0.5633783547074773 1.9771525302294424 8 0.38085874260453784 1.5275516159327345 9 0.28004775050823166 1.2636202460902615 >10 1.4962417458454735 13.786661506565947 >50 0.120284831683714 4.136715382648683 >100 0.050241316942327745 4.233909012463856 >500 0.0014970404384492498 0.4394207815433807 >1k 6.164284158319014E-4 0.6711177836069101 >5k 8.80612022617002E-5 0.39500630287027333 >10k+ 1.761224045234004E-4 1.1246222815485047 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14159 0.6245511824082912 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11314 0.49905869607792974 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8947 0.3946507118445498 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4293 0.1893635303396281 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2652 0.11697928778492749 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2534 0.11177432701621655 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 4.4109837022974166E-5 2.2054918511487083E-5 14-15 0.0 0.0 0.0 4.4109837022974166E-5 6.616475553446125E-5 16-17 0.0 0.0 0.0 4.4109837022974166E-5 8.821967404594833E-5 18-19 0.0 0.0 0.0 1.323295110689225E-4 8.821967404594833E-5 20-21 0.0 0.0 0.0 2.4260410362635793E-4 8.821967404594833E-5 22-23 0.0 0.0 0.0 7.498672293905609E-4 8.821967404594833E-5 24-25 0.0 0.0 0.0 0.001323295110689225 8.821967404594833E-5 26-27 0.0 0.0 0.0 0.002690700058401424 8.821967404594833E-5 28-29 0.0 0.0 0.0 0.005756333731498128 8.821967404594833E-5 30-31 0.0 0.0 0.0 0.01594570608380516 8.821967404594833E-5 32-33 0.0 0.0 0.0 0.03621417619586179 8.821967404594833E-5 34-35 0.0 0.0 0.0 0.06755421540068493 8.821967404594833E-5 36-37 0.0 0.0 0.0 0.12172109526489722 8.821967404594833E-5 38-39 0.0 0.0 0.0 0.20211127323926764 8.821967404594833E-5 40-41 0.0 0.0 0.0 0.2895590251373139 8.821967404594833E-5 42-43 0.0 0.0 0.0 0.3767421180132223 8.821967404594833E-5 44-45 0.0 0.0 0.0 0.4833555940977509 8.821967404594833E-5 46-47 0.0 0.0 0.0 0.6081643779542564 8.821967404594833E-5 48-49 0.0 0.0 0.0 0.7484777695243372 8.821967404594833E-5 50-51 0.0 0.0 0.0 0.8987820391801217 1.7643934809189667E-4 52-53 0.0 0.0 0.0 1.046682322718154 1.7643934809189667E-4 54-55 0.0 0.0 0.0 1.2079037770371246 1.7643934809189667E-4 56-57 0.0 0.0 0.0 1.3923931703857142 1.7643934809189667E-4 58-59 0.0 0.0 0.0 1.6014737978746116 1.7643934809189667E-4 60-61 0.0 0.0 0.0 1.8297642593870145 1.7643934809189667E-4 62-63 0.0 0.0 0.0 2.0545259339375797 1.7643934809189667E-4 64-65 0.0 0.0 0.0 2.285727644693498 1.7643934809189667E-4 66-67 0.0 0.0 0.0 2.547541582343362 1.7643934809189667E-4 68-69 0.0 0.0 0.0 2.841886524797668 1.7643934809189667E-4 70-71 0.0 0.0 0.0 3.1691815155081366 1.7643934809189667E-4 72-73 0.0 0.0 0.0 3.4951532111079153 1.7643934809189667E-4 74-75 0.0 0.0 0.0 3.8253153412248775 1.9849426660338375E-4 76-77 0.0 0.0 0.0 4.175834161127941 2.2054918511487084E-4 78-79 0.0 0.0 0.0 4.565809230248057 2.2054918511487084E-4 80-81 0.0 0.0 0.0 4.992196969830636 2.2054918511487084E-4 82-83 0.0 0.0 0.0 5.421363629145663 2.2054918511487084E-4 84-85 0.0 0.0 0.0 5.856529226295814 2.2054918511487084E-4 86-87 0.0 0.0 0.0 6.305412982760112 2.4260410362635793E-4 88-89 0.0 0.0 0.0 6.795451217166843 2.64659022137845E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6420 0.0 51.38268 1 GTATCAA 11470 0.0 40.52917 1 TATCAAC 14965 0.0 30.873213 2 ATCAACG 14915 0.0 30.830854 3 TCAACGC 15150 0.0 30.41533 4 CAACGCA 15410 0.0 29.840506 5 AACGCAG 15945 0.0 28.913977 6 ACGCAGA 18260 0.0 25.066248 7 CGCAGAG 18470 0.0 24.781252 8 TACATGG 13270 0.0 23.927528 2 GTACATG 13560 0.0 23.8715 1 GCAGAGT 19885 0.0 22.611921 9 ACATGGG 13665 0.0 22.59629 3 GAGTACT 11200 0.0 21.76885 12-13 GTGGTAT 3220 0.0 21.699701 1 TGGTATC 3250 0.0 21.499395 2 GTACTTT 11545 0.0 20.994951 14-15 AGTACTT 11705 0.0 20.829657 12-13 CATGGGG 5530 0.0 20.788525 4 CAGAGTA 19440 0.0 19.71017 10-11 >>END_MODULE