FastQCFastQC Report
Thu 26 May 2016
SRR938486_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938486_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2254344
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146270.6488362024606715No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104880.4652351193961525No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84480.37474316253420065No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41080.1822259601906364No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24620.10921137146770858No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23820.10566266727704378No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62300.047.2966651
GTATCAA111600.040.5643271
TATCAAC145450.030.88022
ATCAACG146850.030.5534363
TCAACGC147700.030.3776054
CAACGCA150250.029.8620455
AACGCAG155700.028.901946
GTACATG135600.026.855281
TACATGG135050.026.4306222
ACGCAGA177050.025.2791657
CGCAGAG178950.025.0113188
ACATGGG137450.024.862583
CATGGGG53900.023.1033274
GCAGAGT187500.022.9798019
AGTACTT111500.021.44900112-13
GAGTACT106750.021.4022612-13
TAGGCCG1805.144775E-621.1241635
CATGGGA82850.020.59514
GTACTTT112450.020.23471314-15
AGAGTAC181650.020.12262210-11
Produced by FastQC (version 0.11.5)