##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938486_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2254344 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.01998009176949 31.0 31.0 34.0 28.0 34.0 2 31.105275858520262 31.0 31.0 34.0 27.0 34.0 3 31.153519161228278 31.0 31.0 34.0 27.0 34.0 4 34.80142338525088 37.0 35.0 37.0 32.0 37.0 5 34.83186328262235 37.0 35.0 37.0 32.0 37.0 6 34.87097532585976 37.0 35.0 37.0 32.0 37.0 7 34.78759053631566 37.0 35.0 37.0 32.0 37.0 8 34.76075878393005 37.0 35.0 37.0 32.0 37.0 9 36.241229821180795 39.0 35.0 39.0 32.0 39.0 10-11 36.08868433566483 39.0 35.0 39.0 30.0 39.0 12-13 35.9293049330537 38.0 35.0 39.0 30.0 39.0 14-15 36.86872921790108 39.0 36.0 41.0 30.5 41.0 16-17 36.734846145929815 39.0 36.0 41.0 30.0 41.0 18-19 36.76983193336953 39.0 36.0 40.5 30.0 41.0 20-21 36.880570578403294 39.0 36.0 41.0 30.0 41.0 22-23 36.786808047041625 39.0 36.0 41.0 30.0 41.0 24-25 36.6704939441363 39.0 36.0 41.0 30.0 41.0 26-27 36.47976240538267 39.0 35.5 41.0 30.0 41.0 28-29 36.2900890458599 39.0 35.0 40.5 29.0 41.0 30-31 36.043453217432656 38.5 35.0 40.0 29.0 41.0 32-33 35.73782639206794 38.0 35.0 40.0 27.0 41.0 34-35 35.478371313339935 38.0 34.5 40.0 27.0 41.0 36-37 35.18040946723304 38.0 34.0 40.0 25.5 41.0 38-39 34.92407103796049 38.0 34.0 40.0 25.0 41.0 40-41 34.810384085126316 38.0 34.0 40.0 25.0 41.0 42-43 34.617535522528954 38.0 33.0 40.0 24.0 41.0 44-45 34.27594391095592 38.0 33.0 40.0 23.5 41.0 46-47 33.860879927819354 38.0 33.0 40.0 22.0 41.0 48-49 33.46882729521315 38.0 32.5 40.0 19.5 41.0 50-51 32.73437616441856 37.0 31.5 39.5 17.5 40.5 52-53 32.817254820027465 37.0 31.5 39.5 18.0 40.0 54-55 32.95662152714936 37.0 32.0 40.0 16.0 41.0 56-57 32.677244688477 37.0 31.5 40.0 13.5 41.0 58-59 32.35698278523597 36.5 31.0 40.0 10.0 41.0 60-61 31.921995711390984 36.0 31.0 39.0 8.0 41.0 62-63 31.403111725628385 35.5 30.0 39.0 6.5 40.0 64-65 30.930091636413962 35.0 29.0 39.0 2.0 40.0 66-67 30.40647988949335 35.0 28.5 38.0 2.0 40.0 68-69 29.8481387490108 34.5 28.0 38.0 2.0 40.0 70-71 29.237596391677577 34.0 27.5 37.0 2.0 39.5 72-73 28.6796121177602 34.0 26.0 36.5 2.0 39.0 74-75 28.071056369391716 33.5 26.0 36.0 2.0 39.0 76-77 27.337160610802965 33.0 25.0 35.5 2.0 38.0 78-79 26.551998275329765 32.0 22.5 35.0 2.0 37.0 80-81 25.87968251517958 32.0 20.5 35.0 2.0 37.0 82-83 25.210093490611904 31.0 19.0 35.0 2.0 36.0 84-85 24.53244735497333 31.0 15.0 34.0 2.0 36.0 86-87 23.83946327623468 31.0 7.5 34.0 2.0 35.0 88-89 23.043332117902146 30.0 2.0 34.0 2.0 35.0 90-91 22.19247373071723 29.0 2.0 34.0 2.0 35.0 92-93 21.32237049891232 29.0 2.0 34.0 2.0 35.0 94-95 20.347961979183303 28.0 2.0 34.0 2.0 35.0 96-97 19.10704377858925 25.5 2.0 33.0 2.0 35.0 98-99 17.650681085051794 22.5 2.0 33.0 2.0 34.5 100-101 14.317223103483764 2.0 2.0 29.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.7547428844164585 1101 2 0.7294120215983142 1101 3 0.7738257462721165 1101 4 0.7110550856133813 1101 5 0.6732293028602214 1101 6 0.7381125531002724 1101 7 0.7512692842466535 1101 8 0.8522339446907381 1101 9 0.7918395027668197 1101 10-11 0.8483771891669463 1101 12-13 0.896926063017105 1101 14-15 1.071973787330542 1101 16-17 1.129552660673852 1101 18-19 1.1463459091316963 1101 20-21 1.0790429079678958 1101 22-23 1.173205841188043 1101 24-25 1.2307587653504939 1101 26-27 1.241671318951603 1101 28-29 1.2491099513845043 1101 30-31 1.3065869823646423 1101 32-33 1.3435504718187374 1101 34-35 1.419078365721198 1101 36-37 1.2406681843122271 1101 38-39 1.2673621352577626 1101 40-41 1.2829601878813222 1101 42-43 1.3290390972641077 1101 44-45 1.4166719495017972 1101 46-47 1.4763193535183845 1101 48-49 1.5388846500810658 1101 50-51 1.4155872975312747 1101 52-53 1.421487341170696 1101 54-55 1.4194646513335556 1101 56-57 1.1872631313905941 1101 58-59 1.3045496350504777 1101 60-61 1.402458367984508 1101 62-63 1.3310715290885433 1101 64-65 1.3264261725252737 1101 66-67 1.2091345926125463 1101 68-69 1.3070114303292577 1101 70-71 1.5547554769398708 1101 72-73 1.471087777118754 1101 74-75 1.314724958655347 1101 76-77 1.5656554977603925 1101 78-79 1.3736793732948094 1101 80-81 1.5645103019601265 1101 82-83 1.637058907666269 1101 84-85 1.7925842445753233 1101 86-87 1.8297288594952938 1101 88-89 1.807479223928187 1101 90-91 1.7155223993556916 1101 92-93 1.6249479791323829 1101 94-95 1.711158171816539 1101 96-97 1.7161184462227048 1101 98-99 1.7340804108380397 1101 100-101 1.2819148621342276 1102 1 0.6455760804936332 1102 2 0.7215781843720244 1102 3 0.5989395293031627 1102 4 0.5437893772273554 1102 5 0.5795991984431765 1102 6 0.5575992988078795 1102 7 0.5949717389451408 1102 8 0.6607653223796817 1102 9 0.8271273638319485 1102 10-11 0.7693108939759341 1102 12-13 0.8708492535388999 1102 14-15 0.7979454678949764 1102 16-17 0.9102301105567463 1102 18-19 0.89313318441544 1102 20-21 0.7441236843818047 1102 22-23 0.9874647556267391 1102 24-25 0.8577701497021764 1102 26-27 0.8124476909781393 1102 28-29 0.7932864283771153 1102 30-31 0.7501068863690747 1102 32-33 0.8204867840295123 1102 34-35 0.8349264971297785 1102 36-37 0.9790884170800567 1102 38-39 1.1336372961731698 1102 40-41 1.1978442692374642 1102 42-43 1.3173694156146425 1102 44-45 1.196330563186656 1102 46-47 1.0908997498025172 1102 48-49 0.8416112553500241 1102 50-51 1.0237976121223937 1102 52-53 0.7998228985191176 1102 54-55 0.8457372718467937 1102 56-57 0.857384233199312 1102 58-59 0.9753009520117146 1102 60-61 0.9040710115567734 1102 62-63 0.9711877362029675 1102 64-65 0.9168166825877755 1102 66-67 1.0096283425718333 1102 68-69 0.9060179050978512 1102 70-71 0.9873738685209972 1102 72-73 0.9566093458775775 1102 74-75 0.9067499802208516 1102 76-77 0.8553711495224334 1102 78-79 0.7756700994310783 1102 80-81 1.0006461734623038 1102 82-83 1.2121189514207416 1102 84-85 1.0875549462009033 1102 86-87 1.0638026083937575 1102 88-89 1.3807813419535968 1102 90-91 1.410291218105904 1102 92-93 1.323033162632239 1102 94-95 1.4792078222553435 1102 96-97 1.5000450286001303 1102 98-99 1.3426812075751577 1102 100-101 1.2348232038657763 1103 1 0.6346145064940316 1103 2 0.6112702277361599 1103 3 0.6048069738050685 1103 4 0.5845643496516928 1103 5 0.60673413504243 1103 6 0.5700886684963251 1103 7 0.52117023317453 1103 8 0.49464972574610755 1103 9 0.5401647900623487 1103 10-11 0.6151234043071128 1103 12-13 0.6110759035249984 1103 14-15 0.8296923754104952 1103 16-17 0.7877184011494691 1103 18-19 0.8057741814555683 1103 20-21 0.8249621351105603 1103 22-23 0.8060065006774053 1103 24-25 0.9153134276448824 1103 26-27 0.8649788241084195 1103 28-29 0.81789080795469 1103 30-31 0.8424414734033618 1103 32-33 0.9929984048047018 1103 34-35 1.0454302642152768 1103 36-37 0.8556876169981678 1103 38-39 0.8064867160096512 1103 40-41 0.6783439902679547 1103 42-43 0.6606397359620502 1103 44-45 0.7910732841653001 1103 46-47 0.7393643760697941 1103 48-49 0.5861833152351821 1103 50-51 0.6472067214629362 1103 52-53 0.7270202764273037 1103 54-55 0.71030954001737 1103 56-57 0.6986441561159751 1103 58-59 0.6879288979762705 1103 60-61 0.7589191205246095 1103 62-63 0.7909714384452293 1103 64-65 0.6075191539607161 1103 66-67 0.6012690511145067 1103 68-69 0.6929875694348233 1103 70-71 0.7717910393934808 1103 72-73 0.713646180045604 1103 74-75 0.5606015542603302 1103 76-77 0.6755741923507905 1103 78-79 0.7494902844151206 1103 80-81 0.7876196046631918 1103 82-83 0.7594137005453874 1103 84-85 0.6945499460214251 1103 86-87 0.9052475508028373 1103 88-89 0.9084099593062511 1103 90-91 1.1149101466414066 1103 92-93 1.0598126889734765 1103 94-95 1.2196466353120918 1103 96-97 1.2182584582233673 1103 98-99 1.349450926775397 1103 100-101 0.9953237801959567 1104 1 0.5566576552256137 1104 2 0.5016931165267309 1104 3 0.6823048411417467 1104 4 0.5712400291451658 1104 5 0.6138082991253668 1104 6 0.6478217544930303 1104 7 0.5782201966721559 1104 8 0.44392097209958337 1104 9 0.5116206132831209 1104 10-11 0.6286029818552521 1104 12-13 0.6269435125368688 1104 14-15 0.8022178178400381 1104 16-17 0.8185082904376202 1104 18-19 0.7981208789134726 1104 20-21 0.8638012111869315 1104 22-23 0.8996443131481158 1104 24-25 0.8346875126994391 1104 26-27 0.9295078501759093 1104 28-29 1.0343423488808199 1104 30-31 0.9192021879420764 1104 32-33 0.9165240249738602 1104 34-35 0.7286945519990482 1104 36-37 0.8408036207328209 1104 38-39 0.9212956925815305 1104 40-41 1.0702794686653831 1104 42-43 1.011184047636739 1104 44-45 0.9800301180415332 1104 46-47 1.2169847734514647 1104 48-49 1.1964879287024175 1104 50-51 0.7484652791589497 1104 52-53 0.8603860751675114 1104 54-55 1.049291550137923 1104 56-57 1.0892365126336614 1104 58-59 0.9459232569704312 1104 60-61 1.0558253058209246 1104 62-63 1.285062338473697 1104 64-65 1.2266711917518052 1104 66-67 1.203345253476364 1104 68-69 1.3605877579491157 1104 70-71 1.2829700766386232 1104 72-73 1.3367206851849964 1104 74-75 1.2064986324161318 1104 76-77 1.265281477681416 1104 78-79 1.3484109722462314 1104 80-81 1.3964123708728131 1104 82-83 1.1948013192629432 1104 84-85 1.260120700176138 1104 86-87 1.0319795046108986 1104 88-89 0.9447782850032844 1104 90-91 1.080396845651876 1104 92-93 1.002089308662871 1104 94-95 0.7232539223819359 1104 96-97 1.0101051092314144 1104 98-99 0.7481348900380205 1104 100-101 0.4981020752615404 1105 1 0.5100402868411393 1105 2 0.5267824925955971 1105 3 0.47367444126644287 1105 4 0.5151250348156609 1105 5 0.43645942291970385 1105 6 0.4477218027414409 1105 7 0.425472989837516 1105 8 0.3368167245920475 1105 9 0.5761581897986332 1105 10-11 0.5992645515717925 1105 12-13 0.6454833974236251 1105 14-15 0.7022077141963408 1105 16-17 0.6148643299860552 1105 18-19 0.6890597785844861 1105 20-21 0.6649413159836115 1105 22-23 0.7141860074508273 1105 24-25 0.7033850385320264 1105 26-27 0.8748903243111812 1105 28-29 0.727871115742154 1105 30-31 0.7303297344921518 1105 32-33 0.753990344487633 1105 34-35 0.9325146733954384 1105 36-37 0.85642730881586 1105 38-39 0.8395824239463323 1105 40-41 0.8709168016891127 1105 42-43 0.9681090810109012 1105 44-45 0.7546272678900223 1105 46-47 0.7672388509646879 1105 48-49 0.7819304060274774 1105 50-51 0.7266574781818491 1105 52-53 0.7862120731483913 1105 54-55 0.7315893035134025 1105 56-57 0.9168378192376565 1105 58-59 0.9506630308978856 1105 60-61 1.12050770792505 1105 62-63 1.1229170356235052 1105 64-65 1.202155493742687 1105 66-67 1.0143825100096855 1105 68-69 1.0691156148799124 1105 70-71 1.169834074363198 1105 72-73 0.9438727391975057 1105 74-75 0.8998735346437954 1105 76-77 0.8267209717198689 1105 78-79 0.9885783392045475 1105 80-81 1.0456464021945102 1105 82-83 1.1194252246848002 1105 84-85 0.9563447094064124 1105 86-87 1.1224014914251406 1105 88-89 1.16987220068145 1105 90-91 1.2556471568963268 1105 92-93 1.1057155324993566 1105 94-95 1.051833655396667 1105 96-97 0.6479865016942945 1105 98-99 0.7455582600270212 1105 100-101 0.637233768523755 1106 1 0.3084412006047046 1106 2 0.3806106081313061 1106 3 0.23384611805719402 1106 4 0.39515462829471204 1106 5 0.3709254423371817 1106 6 0.13573125188738544 1106 7 0.2096309601748061 1106 8 0.30354136678784727 1106 9 0.4076743796814739 1106 10-11 0.16839604353310023 1106 12-13 0.29262226179549344 1106 14-15 0.5467554185384458 1106 16-17 0.3257741131402696 1106 18-19 0.03312291133979528 1106 20-21 0.44840055139886914 1106 22-23 0.4946339765766865 1106 24-25 0.45343912018233823 1106 26-27 0.24995738555756475 1106 28-29 0.2307417697407388 1106 30-31 0.225821488122115 1106 32-33 0.46041135066591465 1106 34-35 0.30625565257616216 1106 36-37 0.18955296861199855 1106 38-39 0.33382813635309816 1106 40-41 0.3528621835488295 1106 42-43 0.010647543880608623 1106 44-45 0.3461442365486249 1106 46-47 0.012140032973235293 1106 48-49 0.4707316105339956 1106 50-51 0.18774248588307785 1106 52-53 0.4808995244748999 1106 54-55 0.26967360166337784 1106 56-57 -0.11250472095581898 1106 58-59 -0.44807881358924817 1106 60-61 0.23321819370806907 1106 62-63 0.4459532227373586 1106 64-65 0.5710615733854922 1106 66-67 0.00813283449958746 1106 68-69 -0.30876152891670827 1106 70-71 -0.2034071903686474 1106 72-73 0.1318129638310488 1106 74-75 0.13572577492207927 1106 76-77 0.17081655346536095 1106 78-79 0.6048668669722268 1106 80-81 0.310916896976714 1106 82-83 0.31335769617295384 1106 84-85 0.5793956067291539 1106 86-87 0.7610516999639856 1106 88-89 0.5218918258307177 1106 90-91 0.5358852892189816 1106 92-93 0.6311122754860463 1106 94-95 0.4191605508503464 1106 96-97 0.5510267557482571 1106 98-99 0.5034087523863668 1106 100-101 0.13417058385006264 1107 1 0.3999541969184115 1107 2 0.37322792068490784 1107 3 0.35910822198280457 1107 4 0.3875923407279771 1107 5 0.4140710037447093 1107 6 0.441699139385868 1107 7 0.5562367424006851 1107 8 0.3043708948436361 1107 9 0.2959316748138576 1107 10-11 0.3795827737782105 1107 12-13 0.32417519668596384 1107 14-15 0.49533141408436165 1107 16-17 0.5842052162891065 1107 18-19 0.45957329456155804 1107 20-21 0.5086827705379591 1107 22-23 0.6471924925409027 1107 24-25 0.6954833961020057 1107 26-27 0.5489713808969441 1107 28-29 0.5403426309233126 1107 30-31 0.733888901027278 1107 32-33 0.5037954434832628 1107 34-35 0.6181823353170444 1107 36-37 0.5706105439205729 1107 38-39 0.5969456801418502 1107 40-41 0.4447983626460683 1107 42-43 0.5821665567983487 1107 44-45 0.714511379604609 1107 46-47 0.6723079815357593 1107 48-49 0.5156013330967042 1107 50-51 0.607787318563112 1107 52-53 0.4637763367523675 1107 54-55 0.6400601235723471 1107 56-57 0.5403203889370047 1107 58-59 0.5531188947132151 1107 60-61 0.4473913372040741 1107 62-63 0.42788208312969545 1107 64-65 0.34115770434989656 1107 66-67 0.520542641564159 1107 68-69 0.33750986033512476 1107 70-71 0.47160863689195764 1107 72-73 0.3901632469257379 1107 74-75 0.5526618417692433 1107 76-77 0.5066603597307129 1107 78-79 0.42575166696504496 1107 80-81 0.48645300333590313 1107 82-83 0.6158491988422625 1107 84-85 0.5538822871352309 1107 86-87 0.4829998449192452 1107 88-89 0.46982799594794855 1107 90-91 0.5150527977612143 1107 92-93 0.6446844320793907 1107 94-95 0.7726820602097568 1107 96-97 0.7824420720465177 1107 98-99 0.6583604399539986 1107 100-101 0.45481180117967845 1108 1 0.3731931906960604 1108 2 0.41283818447653076 1108 3 0.31651382742173695 1108 4 0.35246767723672434 1108 5 0.37855243073340006 1108 6 0.40658370260408816 1108 7 0.28171197116984814 1108 8 0.2799311158020359 1108 9 0.18204781921328106 1108 10-11 0.2654616852819984 1108 12-13 0.37809768380937925 1108 14-15 0.4690849743521639 1108 16-17 0.3492807723935414 1108 18-19 0.4833084723850902 1108 20-21 0.6028447120647584 1108 22-23 0.48269587696891136 1108 24-25 0.49230504309803536 1108 26-27 0.5455264469637342 1108 28-29 0.34062039036596303 1108 30-31 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68-69 -0.6892331755964491 2316 70-71 -1.1699240349689823 2316 72-73 -0.7576365598689492 2316 74-75 -0.6720797737120598 2316 76-77 -0.608054542056589 2316 78-79 -0.7133985406353673 2316 80-81 -0.7887895816829626 2316 82-83 -0.601632922644356 2316 84-85 -0.45958741343666176 2316 86-87 -0.5384473491397443 2316 88-89 -0.7323281091292557 2316 90-91 -0.736312228867213 2316 92-93 -0.8860230243817036 2316 94-95 -0.8553199701513634 2316 96-97 -0.9744560397376993 2316 98-99 -0.8078439283318453 2316 100-101 -0.6275361950926435 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7447.0 3 2661.0 4 2794.0 5 3095.0 6 3661.0 7 4537.0 8 5739.0 9 7339.0 10 9267.0 11 11701.0 12 14725.0 13 17741.0 14 20476.0 15 24027.0 16 27592.0 17 32766.0 18 30200.0 19 26448.0 20 27932.0 21 30806.0 22 33439.0 23 37256.0 24 41735.0 25 46853.0 26 52634.0 27 59206.0 28 67655.0 29 77584.0 30 89881.0 31 104140.0 32 123239.0 33 146792.0 34 179108.0 35 221522.0 36 256577.0 37 263736.0 38 133764.0 39 8269.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.74404529133634 19.402173913043477 14.624142344199937 22.229638451420243 2 15.191792452034688 20.640161757963966 41.05345051554581 23.114595274455528 3 18.979284892310464 25.935934367764723 29.86358127230357 25.22119946762124 4 12.495861312929126 16.70702533808524 37.65792475356504 33.13918859542059 5 13.157414669416811 38.50361278186645 33.86640674344522 14.472565805271524 6 31.029194391054805 36.606668057509886 17.84772930047984 14.516408250955465 7 27.90105312111159 33.70728629167532 20.762132725565476 17.629527861647613 8 26.473161947750086 35.28111596172091 19.719518591522547 18.526203499006456 9 27.298204163613843 16.673937229378634 20.244264280442835 35.78359432656468 10-11 24.883739610455024 27.442222613738586 27.123696439119232 20.550341336687158 12-13 25.802192847573263 24.72884512720016 27.879194130513795 21.589767894712786 14-15 22.85211328542921 25.887152994259228 26.76080494193773 24.49992877837383 16-17 21.541671843660698 29.17197678340049 27.264661247093485 22.02169012584533 18-19 21.41158896952594 27.474293565364494 30.30657784288504 20.807539622224525 20-21 22.567225254393566 26.563204221640568 30.334165130855546 20.53540539311032 22-23 21.933669548692237 26.55388281251907 30.224276962043405 21.288170676745285 24-25 21.45597143740513 26.824309012616432 30.461685154921582 21.258034395056857 26-27 21.28861511312631 27.08038831814464 30.39322249646154 21.23777407226751 28-29 21.19984555916016 27.262735821842725 30.43125829342872 21.10616032556839 30-31 21.57017957913934 27.06560615976354 30.33722601592891 21.02698824516821 32-33 21.245842225480366 27.08733096229138 30.210946100773644 21.455880711454608 34-35 21.570776478629142 27.27385900403207 30.143641112469684 21.011723404869105 36-37 21.670039234813512 27.070889614612987 30.081103625309197 21.177967525264304 38-39 21.446437207981507 27.27948582559424 30.042063974513134 21.232012991911116 40-41 21.536509232410943 27.175767444852898 30.104958635515555 21.182764687220605 42-43 21.355931376170318 27.227331687685393 29.879247421104626 21.537489515039667 44-45 21.418940424819315 27.447967785330167 29.781069334513326 21.352022455337192 46-47 21.51208199363154 27.451984237454123 29.342469369398344 21.69346439951599 48-49 21.535371791604252 27.550218922761154 29.265577853387963 21.648831432246627 50-51 21.57570154446141 27.755854031993856 29.01725993128678 21.65118449225795 52-53 21.609724545734032 27.85876242330193 28.890246396504903 21.641266634459132 54-55 21.3744538811262 27.684988341182777 29.05013025223505 21.890427525455973 56-57 21.525239397917925 28.037022471274582 28.656836329702912 21.780901801104587 58-59 21.567073845657653 27.725910252335034 28.91590451386848 21.79111138813883 60-61 21.81407050558296 27.590301367602283 29.031081708768635 21.56454641804612 62-63 21.562402199036978 27.611062524079415 29.036364269508457 21.79017100737515 64-65 21.428492211188473 27.62431821559359 29.06849229991194 21.878697273305992 66-67 21.53062195448977 27.820545323358353 28.680103121206457 21.96872960094542 68-69 21.514525799033734 27.599739058432142 28.830804768727113 22.054930373807004 70-71 21.46309268287272 27.478012013111265 28.821395096032788 22.23750020798323 72-73 21.500089623545616 27.655940532559082 28.610187268842243 22.233782575053066 74-75 21.296573804309993 27.73414267241178 28.566782564365816 22.402500958912405 76-77 21.31012410571923 27.58539532911041 28.653718690745443 22.450761874424924 78-79 21.32953101457543 27.670142267555097 28.555319235298306 22.445007482571167 80-81 21.263296255976606 27.771264028221022 28.41554853460598 22.549891181196394 82-83 21.43495468175419 27.694646207598222 28.36478797919196 22.50561113145563 84-85 21.485483506777705 27.43408056590524 28.325360910537484 22.75507501677957 86-87 21.26619178023852 27.410317755715784 28.504121060105504 22.819369403940193 88-89 21.4126208971834 27.270686111830322 28.476945642332257 22.839747348654022 90-91 21.39762068689314 27.248608864879447 28.485148763094 22.86862168513341 92-93 21.545947327391097 27.112168777389744 28.33119399203748 23.01068990318168 94-95 21.31244472666295 27.130383617482106 28.369992895726032 23.187178760128916 96-97 21.3624928832516 26.879010174010322 28.295060055815718 23.463436886922356 98-99 21.2701872765196 26.783861093523786 28.21080798799201 23.7351436419646 100-101 21.795018702737945 26.463419292200633 28.286014651292312 23.455547353769106 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3194.0 1 2097.5 2 1439.5 3 1860.0 4 1501.5 5 1363.5 6 2348.0 7 4890.0 8 7315.0 9 7421.5 10 5728.0 11 4308.0 12 3999.0 13 3983.5 14 4013.5 15 4013.5 16 4049.0 17 4179.5 18 4349.5 19 4546.0 20 5034.0 21 5795.5 22 6577.0 23 7826.0 24 9415.0 25 11408.0 26 14452.5 27 18076.0 28 20661.0 29 23506.5 30 27313.0 31 31149.5 32 35961.5 33 41010.0 34 46112.5 35 51331.5 36 57610.0 37 63168.5 38 65693.0 39 67846.5 40 69967.5 41 71975.0 42 73700.5 43 75727.5 44 78620.5 45 80841.5 46 81358.5 47 81441.0 48 82286.5 49 81780.0 50 79881.5 51 77097.0 52 73971.5 53 71168.5 54 67818.0 55 63068.0 56 57429.5 57 51456.5 58 46186.0 59 41332.0 60 35576.0 61 29665.5 62 23718.5 63 17862.5 64 13363.5 65 10314.5 66 7682.0 67 5849.0 68 4467.0 69 3265.5 70 2503.0 71 1877.0 72 1486.5 73 1262.0 74 1026.0 75 803.5 76 599.5 77 385.5 78 226.5 79 136.0 80 74.5 81 42.0 82 29.5 83 19.5 84 14.0 85 12.0 86 11.5 87 6.0 88 2.0 89 1.5 90 0.5 91 0.5 92 0.5 93 0.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17849982079043836 2 0.048839041424024024 3 0.04706468932869163 4 0.05385158609333802 5 0.06081591806751765 6 0.06715922680833095 7 0.037793699630579894 8 0.03504345388281469 9 0.042806244299893895 10-11 0.044225725976159806 12-13 0.04112060980932812 14-15 0.036152423942397434 16-17 0.03477730106851483 18-19 0.04296150010823548 20-21 0.05285351303971355 22-23 0.049925832082415106 24-25 0.0713067748311704 26-27 0.055137991362454 28-29 0.04675417771200846 30-31 0.05635785842799502 32-33 0.052587360225413694 34-35 0.051411851962255985 36-37 0.04948224405858201 38-39 0.02812348071101837 40-41 0.02657092262760253 42-43 0.023088756640512714 44-45 0.0040588304180728405 46-47 0.011821620835152044 48-49 0.010579574368419373 50-51 0.008539069458787123 52-53 0.009448424907644973 54-55 0.011355853410127292 56-57 0.008849581075470292 58-59 0.011422391613702256 60-61 0.011222777002977363 62-63 0.003748318801389673 64-65 0.00660946155511315 66-67 0.004591136046672557 68-69 0.008849581075470292 70-71 0.024685673526311867 72-73 0.020937354724922193 74-75 0.021669274964246806 76-77 0.0132189231102263 78-79 0.004679853651439176 80-81 0.006121514728896743 82-83 0.005300876884805513 84-85 0.005101262274080619 86-87 0.008472531255212159 88-89 0.0321379523267079 90-91 0.038969207893737604 92-93 0.023731959275070706 94-95 0.034200636637531805 96-97 0.03801549364249644 98-99 0.022667348017871274 100-101 0.12429336427803388 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2254344.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.712805999861224 #Duplication Level Percentage of deduplicated Percentage of total 1 74.26038236788659 39.14473129233921 2 13.761355196476705 14.507992935341171 3 4.995264322473917 7.899431974457874 4 2.345985482340779 4.946539104364814 5 1.2526002455588205 3.3014036869760317 6 0.7749028523461481 2.450838223467697 7 0.514271440256835 1.8976083463069437 8 0.34865476128429623 1.4702856634005628 9 0.25788281198439067 1.2234353974948686 >10 1.3378779679430355 12.984533138179884 >50 0.10631218166852253 3.866534120257006 >100 0.04273494111786723 3.8472671649854364 >500 9.299864419793002E-4 0.2944896944451396 >1k 5.918095539867571E-4 0.6691529603652105 >5k 8.454422199810815E-5 0.37649045682086574 >10k+ 1.690884439962163E-4 1.1192658407973535 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14627 0.6488362024606715 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10488 0.4652351193961525 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8448 0.37474316253420065 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4108 0.1822259601906364 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2462 0.10921137146770858 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2382 0.10566266727704378 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.4358802383309736E-5 2 0.0 0.0 0.0 4.4358802383309736E-5 4.4358802383309736E-5 3 0.0 0.0 0.0 4.4358802383309736E-5 4.4358802383309736E-5 4 0.0 0.0 0.0 4.4358802383309736E-5 4.4358802383309736E-5 5 0.0 0.0 0.0 4.4358802383309736E-5 4.4358802383309736E-5 6 0.0 0.0 0.0 4.4358802383309736E-5 4.4358802383309736E-5 7 0.0 0.0 0.0 4.4358802383309736E-5 4.4358802383309736E-5 8 0.0 0.0 0.0 4.4358802383309736E-5 4.4358802383309736E-5 9 0.0 0.0 0.0 4.4358802383309736E-5 4.4358802383309736E-5 10-11 0.0 0.0 0.0 1.1089700595827434E-4 4.4358802383309736E-5 12-13 0.0 0.0 0.0 1.330764071499292E-4 4.4358802383309736E-5 14-15 0.0 0.0 0.0 1.5525580834158407E-4 4.4358802383309736E-5 16-17 0.0 0.0 0.0 1.7743520953323894E-4 4.4358802383309736E-5 18-19 0.0 0.0 0.0 1.996146107248938E-4 4.4358802383309736E-5 20-21 0.0 0.0 0.0 3.992292214497876E-4 8.871760476661947E-5 22-23 4.4358802383309736E-5 0.0 0.0 8.871760476661947E-4 8.871760476661947E-5 24-25 4.4358802383309736E-5 0.0 0.0 0.0016634550893741152 8.871760476661947E-5 26-27 4.4358802383309736E-5 0.0 0.0 0.002972039759681752 8.871760476661947E-5 28-29 4.4358802383309736E-5 0.0 0.0 0.006054976525321779 8.871760476661947E-5 30-31 4.4358802383309736E-5 0.0 0.0 0.01818710897715699 8.871760476661947E-5 32-33 4.4358802383309736E-5 0.0 0.0 0.04025561316285359 8.871760476661947E-5 34-35 4.4358802383309736E-5 0.0 4.4358802383309736E-5 0.07274843590862796 8.871760476661947E-5 36-37 4.4358802383309736E-5 0.0 4.4358802383309736E-5 0.12360580284109257 8.871760476661947E-5 38-39 4.4358802383309736E-5 0.0 4.4358802383309736E-5 0.2008566571916265 8.871760476661947E-5 40-41 4.4358802383309736E-5 0.0 4.4358802383309736E-5 0.2915704080654949 8.871760476661947E-5 42-43 4.4358802383309736E-5 0.0 4.4358802383309736E-5 0.3828608233703463 1.330764071499292E-4 44-45 4.4358802383309736E-5 0.0 4.4358802383309736E-5 0.48761413519853225 1.330764071499292E-4 46-47 4.4358802383309736E-5 0.0 4.4358802383309736E-5 0.6156114594755725 1.330764071499292E-4 48-49 4.4358802383309736E-5 0.0 4.4358802383309736E-5 0.756960783269989 1.330764071499292E-4 50-51 8.871760476661947E-5 0.0 4.4358802383309736E-5 0.9162976014308375 1.330764071499292E-4 52-53 8.871760476661947E-5 0.0 4.4358802383309736E-5 1.0693576490544476 1.330764071499292E-4 54-55 8.871760476661947E-5 0.0 4.4358802383309736E-5 1.2344389321239349 1.330764071499292E-4 56-57 8.871760476661947E-5 0.0 4.4358802383309736E-5 1.423762300695901 1.330764071499292E-4 58-59 8.871760476661947E-5 0.0 4.4358802383309736E-5 1.6356642996809714 1.330764071499292E-4 60-61 8.871760476661947E-5 0.0 4.4358802383309736E-5 1.8612953480036765 1.330764071499292E-4 62-63 1.330764071499292E-4 0.0 4.4358802383309736E-5 2.091584070576629 1.330764071499292E-4 64-65 1.330764071499292E-4 0.0 4.4358802383309736E-5 2.3259538029688462 1.7743520953323894E-4 66-67 1.330764071499292E-4 0.0 4.4358802383309736E-5 2.588668810083998 1.7743520953323894E-4 68-69 1.330764071499292E-4 0.0 4.4358802383309736E-5 2.8897541812607126 1.7743520953323894E-4 70-71 1.330764071499292E-4 0.0 4.4358802383309736E-5 3.216146249197106 1.7743520953323894E-4 72-73 1.330764071499292E-4 0.0 4.4358802383309736E-5 3.5417620380917905 1.7743520953323894E-4 74-75 1.330764071499292E-4 0.0 4.4358802383309736E-5 3.8699284581235163 1.7743520953323894E-4 76-77 1.330764071499292E-4 0.0 4.4358802383309736E-5 4.219897229526638 1.996146107248938E-4 78-79 1.330764071499292E-4 0.0 4.4358802383309736E-5 4.612605707026079 2.2179401191654868E-4 80-81 1.330764071499292E-4 0.0 4.4358802383309736E-5 5.035433811343788 2.2179401191654868E-4 82-83 1.330764071499292E-4 0.0 4.4358802383309736E-5 5.471458659370531 2.2179401191654868E-4 84-85 1.330764071499292E-4 0.0 4.4358802383309736E-5 5.905243387876917 2.2179401191654868E-4 86-87 1.330764071499292E-4 0.0 4.4358802383309736E-5 6.346879624405148 2.661528142998584E-4 88-89 1.330764071499292E-4 0.0 4.4358802383309736E-5 6.816284471225332 3.1051161668316815E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6230 0.0 47.296665 1 GTATCAA 11160 0.0 40.564327 1 TATCAAC 14545 0.0 30.8802 2 ATCAACG 14685 0.0 30.553436 3 TCAACGC 14770 0.0 30.377605 4 CAACGCA 15025 0.0 29.862045 5 AACGCAG 15570 0.0 28.90194 6 GTACATG 13560 0.0 26.85528 1 TACATGG 13505 0.0 26.430622 2 ACGCAGA 17705 0.0 25.279165 7 CGCAGAG 17895 0.0 25.011318 8 ACATGGG 13745 0.0 24.86258 3 CATGGGG 5390 0.0 23.103327 4 GCAGAGT 18750 0.0 22.979801 9 AGTACTT 11150 0.0 21.449001 12-13 GAGTACT 10675 0.0 21.40226 12-13 TAGGCCG 180 5.144775E-6 21.124163 5 CATGGGA 8285 0.0 20.5951 4 GTACTTT 11245 0.0 20.234713 14-15 AGAGTAC 18165 0.0 20.122622 10-11 >>END_MODULE