FastQCFastQC Report
Thu 26 May 2016
SRR938486_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938486_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2254344
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142020.6299837114477649No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111710.49553218142395306No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91960.40792354671691633No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43100.19118643827206494No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25790.1144013513465558No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24790.10996547110822483No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59250.050.8909071
GTATCAA107550.042.5406341
ATCAACG145700.031.060223
TATCAAC147100.030.9413012
TCAACGC148000.030.5775324
CAACGCA151550.029.8612635
AACGCAG156600.028.9339626
GTACATG134900.025.0314331
ACGCAGA180650.025.0031017
TACATGG132950.024.9693012
CGCAGAG181750.024.7211138
ACATGGG132050.024.011243
GCAGAGT192950.022.7446129
TGGTATC31250.022.21992
GTGGTAT31050.022.0566831
CATGGGA78050.021.9258674
AGTACTT116250.021.69466212-13
GAGTACT110600.020.63429512-13
AGAGTAC187200.020.39865510-11
CATGGGG53800.020.2339364
Produced by FastQC (version 0.11.5)