##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938486_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2254344 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.775624749372767 31.0 31.0 34.0 30.0 34.0 2 31.984538739429297 31.0 31.0 34.0 30.0 34.0 3 32.04429492570788 33.0 31.0 34.0 30.0 34.0 4 35.72822958696632 37.0 35.0 37.0 33.0 37.0 5 35.60257706898326 37.0 35.0 37.0 33.0 37.0 6 35.63783921176182 37.0 35.0 37.0 33.0 37.0 7 35.57644041903099 37.0 35.0 37.0 33.0 37.0 8 35.574070771807676 37.0 35.0 37.0 33.0 37.0 9 37.132953976855354 39.0 37.0 39.0 33.0 39.0 10-11 37.04922252327063 39.0 37.0 39.0 33.0 39.0 12-13 36.93542689137062 39.0 37.0 39.0 33.0 39.0 14-15 37.940314787805235 40.0 37.0 41.0 33.0 41.0 16-17 37.87282774944729 40.0 37.0 41.0 32.5 41.0 18-19 38.017385767212104 40.0 37.0 41.0 33.0 41.0 20-21 38.08957439503465 40.0 37.0 41.0 33.0 41.0 22-23 37.98416368575515 40.0 37.0 41.0 33.0 41.0 24-25 37.88736723410447 40.0 37.0 41.0 32.5 41.0 26-27 37.69634581057727 40.0 37.0 41.0 32.0 41.0 28-29 37.56031089310238 39.5 36.5 41.0 32.0 41.0 30-31 37.3023637918614 39.0 36.0 41.0 31.5 41.0 32-33 37.058439173435815 39.0 35.5 41.0 31.0 41.0 34-35 36.89119406798608 39.0 35.0 40.5 31.0 41.0 36-37 36.86965143740263 39.0 35.0 41.0 31.0 41.0 38-39 36.746656455270355 39.0 35.0 41.0 30.0 41.0 40-41 36.56018890639583 39.0 35.0 40.5 30.0 41.0 42-43 36.337556069526215 39.0 35.0 40.0 30.0 41.0 44-45 36.02628946602648 38.5 35.0 40.0 29.5 41.0 46-47 35.60944137185807 38.0 35.0 40.0 27.5 41.0 48-49 35.2271126323223 38.0 34.0 40.0 27.0 41.0 50-51 34.87581664555188 38.0 34.0 40.0 26.0 41.0 52-53 34.41633197950269 38.0 33.0 40.0 24.0 41.0 54-55 34.12057432228622 38.0 33.0 40.0 24.0 41.0 56-57 33.817216006075384 37.0 33.0 40.0 23.0 41.0 58-59 33.40352625863666 37.0 32.0 40.0 22.0 41.0 60-61 33.05733996231277 36.0 32.0 39.0 21.5 40.5 62-63 32.61688012122374 36.0 31.0 39.0 19.5 40.0 64-65 32.14151677827341 35.0 31.0 39.0 18.0 40.0 66-67 31.67553753996728 35.0 30.5 38.0 16.5 40.0 68-69 31.15860534150955 35.0 29.5 38.0 13.0 40.0 70-71 30.61538079370318 34.0 29.0 37.0 9.0 39.0 72-73 30.183711980070477 34.0 29.0 37.0 8.0 39.0 74-75 29.679832359214032 34.0 28.5 36.0 7.0 39.0 76-77 28.39620217677515 32.5 26.5 35.0 4.0 37.0 78-79 29.112552476463218 33.5 28.5 35.0 2.0 37.0 80-81 28.839564414304117 34.0 28.0 35.0 2.0 37.0 82-83 28.3687145794963 33.0 27.0 35.0 2.0 36.5 84-85 27.845800596537174 33.0 26.0 35.0 2.0 36.0 86-87 27.273041514515974 32.5 26.0 35.0 2.0 36.0 88-89 26.642704707001236 32.0 25.0 34.5 2.0 35.0 90-91 26.096093808220928 32.0 24.0 34.0 2.0 35.0 92-93 25.35855197787028 31.0 21.0 34.0 2.0 35.0 94-95 24.599088692763836 31.0 18.5 34.0 2.0 35.0 96-97 23.6199914476229 31.0 4.5 34.0 2.0 35.0 98-99 22.40243946797827 30.5 2.0 34.0 2.0 35.0 100-101 18.796370695865406 24.5 2.0 32.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.34665355179057755 1101 2 0.3968161803228192 1101 3 0.3257937028892286 1101 4 0.2257686674880972 1101 5 0.11204282941543653 1101 6 0.2735465503458059 1101 7 0.2982414028185758 1101 8 0.3047908396347836 1101 9 0.5097518199150812 1101 10-11 0.5090718982396609 1101 12-13 0.4282748540129333 1101 14-15 0.6396013873110533 1101 16-17 0.44586073300621365 1101 18-19 0.5251160322095885 1101 20-21 0.4750906295359343 1101 22-23 0.5491327635164893 1101 24-25 0.5172820983308668 1101 26-27 0.6857365585428212 1101 28-29 0.6040846618559002 1101 30-31 0.4851902087424236 1101 32-33 0.5038305874523132 1101 34-35 0.339495605056527 1101 36-37 0.5811911217559782 1101 38-39 0.6228551833968794 1101 40-41 0.7003486950445108 1101 42-43 0.8388626396882586 1101 44-45 0.9197831676839527 1101 46-47 0.7853146929548487 1101 48-49 0.7508171254383598 1101 50-51 0.6559103885758901 1101 52-53 0.6632831632870619 1101 54-55 0.8015943930695215 1101 56-57 0.6658088604306371 1101 58-59 0.7582184888707388 1101 60-61 0.6646099844596733 1101 62-63 0.4495068339011752 1101 64-65 0.6673863941797791 1101 66-67 0.5332289194469944 1101 68-69 0.5312562942581778 1101 70-71 0.26002272116760494 1101 72-73 0.3777028710589825 1101 74-75 0.5632278168031171 1101 76-77 0.47626498445946197 1101 78-79 0.4883481224091675 1101 80-81 0.7313550605470063 1101 82-83 0.6020867382685786 1101 84-85 0.5925805192915767 1101 86-87 0.7343669742116781 1101 88-89 0.803812780104824 1101 90-91 0.9254988146784804 1101 92-93 0.8464009786542022 1101 94-95 0.7850702692197302 1101 96-97 0.9442398634161613 1101 98-99 0.7699928235319966 1101 100-101 0.5486905289920578 1102 1 0.37115377651845805 1102 2 0.3042811009050048 1102 3 0.33997901080364556 1102 4 0.26697436282780984 1102 5 0.21602949276934424 1102 6 0.2033188540066817 1102 7 0.3681620175772835 1102 8 0.3614932208197956 1102 9 0.29003099429368007 1102 10-11 0.43042388538317766 1102 12-13 0.36395522117084056 1102 14-15 0.5094467205550544 1102 16-17 0.4966681947163991 1102 18-19 0.5284529853833746 1102 20-21 0.5457805767085873 1102 22-23 0.4739647759337515 1102 24-25 0.558161752128953 1102 26-27 0.4953809906419693 1102 28-29 0.5396359151674943 1102 30-31 0.43032729275157067 1102 32-33 0.39162472275245364 1102 34-35 0.454862199977768 1102 36-37 0.5522795072792235 1102 38-39 0.5524612675204779 1102 40-41 0.5248848390118042 1102 42-43 0.49272353917672973 1102 44-45 0.6437062418869672 1102 46-47 0.46999972030837256 1102 48-49 0.6324491570249506 1102 50-51 0.7483390363095808 1102 52-53 0.6709419407487616 1102 54-55 0.8290926180449247 1102 56-57 0.746723228405564 1102 58-59 0.878695549411411 1102 60-61 0.9386576128127899 1102 62-63 0.8753345350791619 1102 64-65 0.9229994732495683 1102 66-67 1.0367244151185453 1102 68-69 0.9434266584662403 1102 70-71 0.8705034620344883 1102 72-73 0.9013284948765978 1102 74-75 0.8145989825871602 1102 76-77 0.8424859408231704 1102 78-79 0.9840936466951362 1102 80-81 1.1748472806816928 1102 82-83 0.8509207588132668 1102 84-85 0.5930221817009738 1102 86-87 0.4768977963621204 1102 88-89 0.5776096251672804 1102 90-91 0.45851403941101765 1102 92-93 0.47438920812416896 1102 94-95 0.3976423054404705 1102 96-97 0.40396367145109124 1102 98-99 0.29113562298073603 1102 100-101 0.3552118018359032 1103 1 0.23485268621077537 1103 2 0.27096651325916454 1103 3 0.2385034637331387 1103 4 0.21206456592381784 1103 5 0.0677014707141339 1103 6 0.10354748048844442 1103 7 0.12590968112454703 1103 8 0.08182972142094513 1103 9 0.2204596767022906 1103 10-11 0.2512728345832542 1103 12-13 0.20314613868582398 1103 14-15 0.31207080659199704 1103 16-17 0.3167320851600337 1103 18-19 0.4108142684038114 1103 20-21 0.35550209910658737 1103 22-23 0.3594342756366089 1103 24-25 0.3159570554257556 1103 26-27 0.4702437106101982 1103 28-29 0.432735973265828 1103 30-31 0.46839566266115185 1103 32-33 0.4945754402070861 1103 34-35 0.4319919072835745 1103 36-37 0.5556393283133048 1103 38-39 0.5463871994536973 1103 40-41 0.5776564974822094 1103 42-43 0.5868769675325396 1103 44-45 0.6682299662606539 1103 46-47 0.5474108788228804 1103 48-49 0.6155071326059272 1103 50-51 0.6296929048123445 1103 52-53 0.726865024046532 1103 54-55 0.6393811642526117 1103 56-57 0.6996172656372508 1103 58-59 0.7356700530606091 1103 60-61 0.6506886216365615 1103 62-63 0.6293850216915047 1103 64-65 0.7862203235947263 1103 66-67 0.6766001484937156 1103 68-69 0.5350632677746425 1103 70-71 0.38878637921799 1103 72-73 0.23582944523420935 1103 74-75 0.4417019451591564 1103 76-77 0.42111778482714257 1103 78-79 0.48887246752491365 1103 80-81 0.36767681147669506 1103 82-83 0.3053256408461671 1103 84-85 0.31414163604636514 1103 86-87 0.28468381544726995 1103 88-89 0.2378608794221755 1103 90-91 0.2605681103051687 1103 92-93 0.18660224510724177 1103 94-95 0.2093746684296569 1103 96-97 0.21224747052390924 1103 98-99 0.13145172490586887 1103 100-101 0.04226274180139811 1104 1 0.2759266577071138 1104 2 0.30550526622272756 1104 3 0.2695864749222494 1104 4 0.25393730520524826 1104 5 0.21503107080847883 1104 6 0.2129061289207499 1104 7 0.15361882105568014 1104 8 0.2910394333852011 1104 9 0.28001517494377026 1104 10-11 0.38294300160335126 1104 12-13 0.32702800165155566 1104 14-15 0.6009937681327955 1104 16-17 0.5338602243959158 1104 18-19 0.48531749889561837 1104 20-21 0.4557663330170172 1104 22-23 0.4399432175513951 1104 24-25 0.48118045376484986 1104 26-27 0.473323505518195 1104 28-29 0.37840353824578443 1104 30-31 0.3900324282073271 1104 32-33 0.431326118184046 1104 34-35 0.6009865458252506 1104 36-37 0.485605676561633 1104 38-39 0.5078572588119386 1104 40-41 0.7025352294309357 1104 42-43 0.710668794140112 1104 44-45 0.5534883313700689 1104 46-47 0.6483950854487901 1104 48-49 0.7513574682114097 1104 50-51 0.6532276028522475 1104 52-53 0.7303837594567142 1104 54-55 0.6938727801602624 1104 56-57 0.7315026503077746 1104 58-59 0.7852629063159853 1104 60-61 0.6689054478517562 1104 62-63 0.7762150207455392 1104 64-65 0.6859464391441392 1104 66-67 0.7262248167166199 1104 68-69 0.6060194475351501 1104 70-71 0.4569914126852126 1104 72-73 0.3229961993566839 1104 74-75 0.23212095252368314 1104 76-77 0.4075263246431504 1104 78-79 0.4124208884208187 1104 80-81 0.430521977362055 1104 82-83 0.3590171103201314 1104 84-85 0.3962697477092618 1104 86-87 0.46660179985454064 1104 88-89 0.6449374190226891 1104 90-91 0.3416745373103929 1104 92-93 0.3892356972305926 1104 94-95 0.3458740293730074 1104 96-97 0.513978819847587 1104 98-99 0.48427876050172713 1104 100-101 0.27083843660847506 1105 1 0.19831721596255036 1105 2 0.2975037026688021 1105 3 0.21396099509960465 1105 4 0.14633008505709455 1105 5 0.10488347726339953 1105 6 0.15880001213869832 1105 7 0.12061606233913125 1105 8 0.1592750640152687 1105 9 0.21308044367859935 1105 10-11 0.2225924513665447 1105 12-13 0.1714821055989617 1105 14-15 0.3008966687020944 1105 16-17 0.4396545937537937 1105 18-19 0.3872782083762871 1105 20-21 0.41973087424931776 1105 22-23 0.31598185404559587 1105 24-25 0.36705337041561137 1105 26-27 0.37512020654796174 1105 28-29 0.3262193233109443 1105 30-31 0.34049313829060424 1105 32-33 0.42663113774654704 1105 34-35 0.4223545269565463 1105 36-37 0.39392505293983504 1105 38-39 0.2797730261605764 1105 40-41 0.37650762057170084 1105 42-43 0.3320180744915149 1105 44-45 0.3518524449952878 1105 46-47 0.15398613655315074 1105 48-49 0.17834207090119492 1105 50-51 0.19248116252862957 1105 52-53 0.16612639333803259 1105 54-55 0.3115872596257603 1105 56-57 0.2648160508728594 1105 58-59 0.17436015077314693 1105 60-61 0.010493270610943739 1105 62-63 0.18753154359765034 1105 64-65 0.12455766419185466 1105 66-67 0.07311424027060909 1105 68-69 -0.3092950449465732 1105 70-71 -0.15491959811704703 1105 72-73 -0.15792699648005026 1105 74-75 -0.13583758115840894 1105 76-77 -0.09801199869835742 1105 78-79 0.06137480977951526 1105 80-81 0.22215822931599405 1105 82-83 0.066446723276254 1105 84-85 0.011104889588125388 1105 86-87 0.18141691522690095 1105 88-89 0.1838368490004214 1105 90-91 0.08363370412737581 1105 92-93 0.04009966924410335 1105 94-95 0.1653495357704422 1105 96-97 0.17175505005157632 1105 98-99 0.0033155919990655036 1105 100-101 -0.3550496495685742 1106 1 0.19008382551899317 1106 2 0.2334407429747749 1106 3 0.18089763736463738 1106 4 0.06712426327560195 1106 5 -0.03655085463352492 1106 6 -0.05488307804353809 1106 7 0.22643870913836395 1106 8 0.11430941667954642 1106 9 0.026658831785830728 1106 10-11 0.08740995824572906 1106 12-13 0.10818710533936837 1106 14-15 0.27357029243226094 1106 16-17 0.08114517056704074 1106 18-19 0.22412901224593895 1106 20-21 0.18096687791821608 1106 22-23 0.30156368607231343 1106 24-25 0.18307624911695797 1106 26-27 0.17332642637040152 1106 28-29 0.272661183373593 1106 30-31 0.318120641189374 1106 32-33 0.19391346992036773 1106 34-35 0.06584828809736365 1106 36-37 -0.2961935805682856 1106 38-39 -0.02830633974289043 1106 40-41 0.18473192529800997 1106 42-43 0.1696346745295898 1106 44-45 0.08028684611672787 1106 46-47 -0.17899674768616336 1106 48-49 -0.2328082674336116 1106 50-51 -0.146798989073055 1106 52-53 -0.008808276028389628 1106 54-55 -0.3452019344278554 1106 56-57 -0.011480610775024047 1106 58-59 0.142551487325413 1106 60-61 -0.4337658540450917 1106 62-63 -0.20160323652799406 1106 64-65 -0.556817048093766 1106 66-67 -0.2676434220176098 1106 68-69 -1.0330329955548834 1106 70-71 -0.7318712967135212 1106 72-73 -0.44766619312958156 1106 74-75 -0.1326186413318169 1106 76-77 -0.1396483796408461 1106 78-79 -0.20271454175986037 1106 80-81 -0.8943033597326853 1106 82-83 -0.25427909573625485 1106 84-85 0.12325941600592927 1106 86-87 0.07552504391527393 1106 88-89 0.05750211387071147 1106 90-91 -0.10467427572828925 1106 92-93 -0.16448039755755062 1106 94-95 -0.23263949214434376 1106 96-97 -0.3727498216234828 1106 98-99 -0.3963629466714771 1106 100-101 -0.7636841095308462 1107 1 0.19181359917794438 1107 2 0.15312244666151642 1107 3 0.08649981411809193 1107 4 0.12132421208956856 1107 5 -0.028352109140364234 1107 6 0.0063033154513476575 1107 7 0.002837681825674565 1107 8 0.10307206165051497 1107 9 0.1310311967153126 1107 10-11 0.150833495739505 1107 12-13 0.1324381728375883 1107 14-15 0.31897095785070206 1107 16-17 0.2553831048064552 1107 18-19 0.19483847762128192 1107 20-21 0.1600221363852441 1107 22-23 0.2391386129975146 1107 24-25 0.1939555264060786 1107 26-27 0.1302291324952094 1107 28-29 0.14339736426683913 1107 30-31 0.27694170109948857 1107 32-33 0.329507632018462 1107 34-35 0.289311247817551 1107 36-37 0.3003184728447863 1107 38-39 0.2633867056914525 1107 40-41 0.29027337476077975 1107 42-43 0.34957491258955287 1107 44-45 0.19699044305813374 1107 46-47 0.17197668496603313 1107 48-49 0.10216616199925 1107 50-51 0.14887725028002308 1107 52-53 0.22620184892839745 1107 54-55 0.19273859099229185 1107 56-57 0.16400554430069292 1107 58-59 0.03301449643563359 1107 60-61 0.08228983546221258 1107 62-63 0.22766855233410865 1107 64-65 0.14586449395286394 1107 66-67 -0.12227957462382832 1107 68-69 -0.10650740478273946 1107 70-71 -0.3116855901890965 1107 72-73 -0.3243213204885187 1107 74-75 -0.2750426592559094 1107 76-77 -0.3802401395873254 1107 78-79 -0.14375238350521613 1107 80-81 -0.09202737720778131 1107 82-83 -0.09466285629337534 1107 84-85 -0.0059982293466944725 1107 86-87 0.019484193867903343 1107 88-89 -0.005109855579309652 1107 90-91 0.06751509463858696 1107 92-93 -0.05233583742069925 1107 94-95 -0.1490131861147148 1107 96-97 0.10832088694388986 1107 98-99 0.036174196878409504 1107 100-101 -0.5748766767798692 1108 1 0.12146277839115882 1108 2 0.19928899728456528 1108 3 0.1939313608055997 1108 4 0.16088834233379856 1108 5 0.13573462760670907 1108 6 -0.013864142228221965 1108 7 0.11753064887283671 1108 8 0.07467295962227638 1108 9 0.2275085100110701 1108 10-11 0.12032839727277178 1108 12-13 0.22147822598343225 1108 14-15 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0.09905238050365739 2316 76-77 0.021179006597670025 2316 78-79 0.0475093394728745 2316 80-81 -0.068386058957703 2316 82-83 0.20220648535400798 2316 84-85 0.21596142023544473 2316 86-87 0.400022921702206 2316 88-89 0.41538731204686385 2316 90-91 0.3656285842013851 2316 92-93 0.3049620769450101 2316 94-95 0.29461545240977927 2316 96-97 0.429256377836861 2316 98-99 0.7633128221915122 2316 100-101 0.8995221146336299 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 10.0 4 39.0 5 121.0 6 215.0 7 511.0 8 1073.0 9 1999.0 10 3146.0 11 4916.0 12 6820.0 13 9715.0 14 12622.0 15 16407.0 16 20994.0 17 26309.0 18 24508.0 19 18884.0 20 18507.0 21 19464.0 22 21160.0 23 24007.0 24 28053.0 25 33002.0 26 39457.0 27 47464.0 28 56849.0 29 68157.0 30 81335.0 31 95991.0 32 116893.0 33 144233.0 34 183875.0 35 240753.0 36 309208.0 37 366818.0 38 200668.0 39 10158.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.423513859993875 20.17796862038832 15.304103658293153 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30.416254129804503 20.79587232472063 20-21 22.385780519743214 26.60920427405933 30.429872282136177 20.57514292406128 22-23 21.840677376655915 26.5545320501219 30.348496059164 21.25629451405819 24-25 21.417030205938186 26.815746416414726 30.631517008403563 21.135706369243522 26-27 21.295642360156087 27.14781053307757 30.452417667632588 21.10412943913376 28-29 21.17383150042762 27.28809356513469 30.51135940211432 21.026715532323372 30-31 21.521005667280594 27.085418197045346 30.392145120709174 21.001431014964886 32-33 21.2461807071148 27.04933231130653 30.322612697973334 21.38187428360534 34-35 21.539375080963 27.2748385619906 30.27046966527011 20.91531669177629 36-37 21.574092289962408 27.05578416392732 30.250620968748954 21.119502577361317 38-39 21.412077264362303 27.272868419599245 30.204628080916113 21.110426235122336 40-41 21.49422574705077 27.16305471799474 30.25377612143565 21.088943413518844 42-43 21.410271793699128 27.196714246773503 29.971741155997645 21.421272803529728 44-45 21.42572295976124 27.341656819012538 29.88015582360101 21.352464397625205 46-47 21.471146373401755 27.458897133711623 29.420665169113498 21.649291323773124 48-49 21.47544917723293 27.511240520523934 29.38801709055938 21.625293211683754 50-51 21.60808199635903 27.623734443368004 29.09411784536876 21.674065714904202 52-53 21.639066619823772 27.742749110162425 28.918079938110598 21.700104331903205 54-55 21.42405950467187 27.669934136050223 29.027513103590223 21.878493255687687 56-57 21.528280510871454 27.951745607591388 28.682490338652837 21.837483542884314 58-59 21.60954583683768 27.688453936045253 28.882371102192035 21.819629124925033 60-61 21.810535570436453 27.640435532465318 28.982333663362823 21.566695233735402 62-63 21.599853438516924 27.640657326477236 28.938241013793814 21.821248221212024 64-65 21.380055572707626 27.643496289829766 29.007817795332038 21.96863034213057 66-67 21.511829842956324 27.901568573698476 28.604257297365393 21.982344285979806 68-69 21.495028194855013 27.686030150218784 28.66819233539989 22.150749319526312 70-71 21.46198790201041 27.6252510988242 28.617802582261824 22.29495841690356 72-73 21.46908368909093 27.794538011944937 28.461472605778 22.274905693186135 74-75 21.29379988147328 27.897095563055153 28.35026508820304 22.458839467268525 76-77 21.18915746665105 27.78930367326371 28.601424627297344 22.420114232787896 78-79 21.33290216577417 27.783625746558645 28.535573985159317 22.34789810250787 80-81 21.23226448949898 27.958417339268642 28.384180490217602 22.42513768101477 82-83 21.314863067719575 27.944937866129386 28.31939568633818 22.420803379812856 84-85 21.359928773620688 27.72154992789083 28.24583303944023 22.67268825904825 86-87 21.14894523097124 27.69175148661327 28.38536387368944 22.773939408726047 88-89 21.254073250642538 27.68342159264211 28.2938657932115 22.76863936350385 90-91 21.176641766530786 27.616216591479777 28.500914818822842 22.7062268231666 92-93 21.28397118685758 27.563163767675242 28.233477212736354 22.919387832730823 94-95 21.000939520268 27.649087849137356 28.2249765119933 23.12499611860135 96-97 21.049515956748394 27.40557785324689 28.175225253998505 23.36968093600622 98-99 20.9133880691625 27.44431526169709 28.08620959632141 23.556087072818997 100-101 21.067769603929126 27.457921240059193 28.255980453737315 23.218328702274366 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3269.0 1 2145.5 2 1434.0 3 1817.5 4 1458.0 5 1342.5 6 2383.0 7 5099.0 8 7376.0 9 7389.5 10 5747.5 11 4133.5 12 3845.0 13 3915.0 14 3940.0 15 3900.0 16 3933.5 17 4047.5 18 4235.5 19 4556.5 20 5008.0 21 5685.5 22 6460.5 23 7614.5 24 9257.5 25 11555.0 26 14589.0 27 17967.0 28 20664.0 29 23650.0 30 27655.0 31 31708.5 32 36720.5 33 41600.0 34 46500.0 35 51740.5 36 58131.5 37 64060.0 38 66616.0 39 68183.5 40 70294.5 41 72644.5 42 74156.0 43 76584.5 44 79756.5 45 81992.0 46 82423.5 47 82275.0 48 83329.5 49 82875.0 50 80897.0 51 77284.0 52 73272.0 53 71066.0 54 68135.0 55 62958.0 56 56894.0 57 50761.0 58 45343.5 59 40341.5 60 34506.0 61 28390.0 62 22553.5 63 16808.0 64 12163.0 65 9242.0 66 6842.0 67 5201.0 68 3977.5 69 2793.5 70 2128.5 71 1593.5 72 1277.0 73 1114.5 74 901.0 75 702.5 76 469.0 77 273.5 78 172.0 79 108.5 80 63.0 81 36.5 82 19.0 83 10.5 84 5.5 85 3.0 86 2.5 87 0.5 88 1.0 89 1.5 90 0.5 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 6.210232333663363E-4 2 0.050702111124123025 3 7.984584428995752E-4 4 0.0 5 0.08951606320951905 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.1089700595827434E-4 26-27 0.00833945484806223 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010113806943394619 36-37 1.5525580834158407E-4 38-39 0.003703959999006363 40-41 0.005012544669314 42-43 2.4397341310820355E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.330764071499292E-4 68-69 0.0014194816762659115 70-71 1.1089700595827434E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0017299932929490797 82-83 7.984584428995752E-4 84-85 0.005456132693147097 86-87 0.002617169340615274 88-89 8.871760476661947E-5 90-91 0.0032160131727899553 92-93 0.0011311494607743982 94-95 8.871760476661947E-5 96-97 0.0 98-99 4.4358802383309736E-5 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2254344.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.28749065286742 #Duplication Level Percentage of deduplicated Percentage of total 1 72.02895536984366 36.22155419896818 2 14.772446344870946 14.857385149753663 3 5.502738424529719 8.301567212661379 4 2.6001902645347386 5.230281744938701 5 1.359289115151953 3.4177619336374128 6 0.8751812746257661 2.6406402100384687 7 0.5498471281089123 1.9355302620698043 8 0.37956160705528247 1.5269760613583883 9 0.27133431634828403 1.2280249707449853 >10 1.4927677222359044 13.761197985804385 >50 0.116034319434042 4.017039162202056 >100 0.04936030452527198 4.236633351949133 >500 0.0014115746064938658 0.41423918442288965 >1k 6.175638903409466E-4 0.677503115060605 >5k 8.822341290584951E-5 0.40798367140968655 >10k+ 1.7644682581169902E-4 1.1256817849802063 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14202 0.6299837114477649 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11171 0.49553218142395306 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9196 0.40792354671691633 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4310 0.19118643827206494 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2579 0.1144013513465558 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2479 0.10996547110822483 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 4.4358802383309736E-5 7 0.0 0.0 0.0 0.0 4.4358802383309736E-5 8 0.0 0.0 0.0 0.0 4.4358802383309736E-5 9 0.0 0.0 0.0 0.0 4.4358802383309736E-5 10-11 0.0 0.0 0.0 4.4358802383309736E-5 4.4358802383309736E-5 12-13 0.0 0.0 0.0 4.4358802383309736E-5 8.871760476661947E-5 14-15 0.0 0.0 0.0 6.65382035749646E-5 1.1089700595827434E-4 16-17 0.0 0.0 0.0 1.330764071499292E-4 1.7743520953323894E-4 18-19 0.0 0.0 0.0 1.330764071499292E-4 1.7743520953323894E-4 20-21 0.0 0.0 0.0 3.1051161668316815E-4 2.661528142998584E-4 22-23 0.0 0.0 0.0 7.762790417079204E-4 2.661528142998584E-4 24-25 0.0 0.0 0.0 0.0015303786822241858 2.661528142998584E-4 26-27 0.0 0.0 0.0 0.0027946045501485136 2.661528142998584E-4 28-29 0.0 0.0 0.0 0.005833182513405231 2.661528142998584E-4 30-31 0.0 0.0 0.0 0.018076211971198717 2.661528142998584E-4 32-33 0.0 0.0 0.0 0.040455227773578475 2.661528142998584E-4 34-35 0.0 0.0 0.0 0.07341381794437761 2.661528142998584E-4 36-37 0.0 0.0 0.0 0.12493656691259186 2.661528142998584E-4 38-39 0.0 0.0 0.0 0.2026975474905338 2.661528142998584E-4 40-41 0.0 0.0 0.0 0.29409885980134354 2.661528142998584E-4 42-43 0.0 0.0 0.0 0.385899401333603 2.661528142998584E-4 44-45 0.0 0.0 0.0 0.49111848058681373 2.661528142998584E-4 46-47 0.0 0.0 0.0 0.6197146486960287 2.661528142998584E-4 48-49 0.0 0.0 0.0 0.7618624309333447 3.1051161668316815E-4 50-51 4.4358802383309736E-5 0.0 0.0 0.9225965513692675 3.1051161668316815E-4 52-53 4.4358802383309736E-5 0.0 0.0 1.0757896754000278 3.548704190664779E-4 54-55 4.4358802383309736E-5 0.0 0.0 1.2405160880504484 3.548704190664779E-4 56-57 4.4358802383309736E-5 0.0 0.0 1.430504838658164 3.548704190664779E-4 58-59 4.4358802383309736E-5 0.0 0.0 1.6431165784813677 3.548704190664779E-4 60-61 4.4358802383309736E-5 0.0 0.0 1.8697900586600804 3.548704190664779E-4 62-63 4.4358802383309736E-5 0.0 0.0 2.1006776250652073 3.548704190664779E-4 64-65 4.4358802383309736E-5 0.0 0.0 2.3357570982955576 3.548704190664779E-4 66-67 4.4358802383309736E-5 0.0 0.0 2.5993814608595676 4.4358802383309736E-4 68-69 4.4358802383309736E-5 0.0 0.0 2.9016866991018233 4.4358802383309736E-4 70-71 4.4358802383309736E-5 0.0 0.0 3.2302523483549983 4.4358802383309736E-4 72-73 4.4358802383309736E-5 0.0 0.0 3.5589732534165153 4.4358802383309736E-4 74-75 4.4358802383309736E-5 0.0 0.0 3.8921522181175545 4.4358802383309736E-4 76-77 4.4358802383309736E-5 0.0 0.0 4.248885706884131 4.4358802383309736E-4 78-79 4.4358802383309736E-5 0.0 0.0 4.647671340310086 4.4358802383309736E-4 80-81 4.4358802383309736E-5 0.0 0.0 5.081544786421238 4.4358802383309736E-4 82-83 4.4358802383309736E-5 0.0 0.0 5.529546511091475 4.4358802383309736E-4 84-85 4.4358802383309736E-5 0.0 0.0 5.972025564864989 4.4358802383309736E-4 86-87 4.4358802383309736E-5 0.0 0.0 6.425838292647439 4.4358802383309736E-4 88-89 4.4358802383309736E-5 0.0 0.0 6.911123590720848 4.4358802383309736E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5925 0.0 50.890907 1 GTATCAA 10755 0.0 42.540634 1 ATCAACG 14570 0.0 31.06022 3 TATCAAC 14710 0.0 30.941301 2 TCAACGC 14800 0.0 30.577532 4 CAACGCA 15155 0.0 29.861263 5 AACGCAG 15660 0.0 28.933962 6 GTACATG 13490 0.0 25.031433 1 ACGCAGA 18065 0.0 25.003101 7 TACATGG 13295 0.0 24.969301 2 CGCAGAG 18175 0.0 24.721113 8 ACATGGG 13205 0.0 24.01124 3 GCAGAGT 19295 0.0 22.744612 9 TGGTATC 3125 0.0 22.2199 2 GTGGTAT 3105 0.0 22.056683 1 CATGGGA 7805 0.0 21.925867 4 AGTACTT 11625 0.0 21.694662 12-13 GAGTACT 11060 0.0 20.634295 12-13 AGAGTAC 18720 0.0 20.398655 10-11 CATGGGG 5380 0.0 20.233936 4 >>END_MODULE