FastQCFastQC Report
Thu 26 May 2016
SRR938485_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938485_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2250947
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146980.6529696167879563No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105580.469047027762093No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84700.3762860698186141No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41310.18352275731058973No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24710.10977601871567833No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23750.1055111470860931No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57000.051.576961
GTATCAA103150.043.2366521
TATCAAC137200.032.1799662
ATCAACG138350.031.8437733
TCAACGC139650.031.5813714
CAACGCA141400.031.1569025
AACGCAG147050.029.9901036
ACGCAGA167700.026.091327
GTACATG132250.025.8326471
CGCAGAG169400.025.801448
TACATGG129950.025.7100542
ACATGGG132750.024.0579553
GCAGAGT179900.023.5809259
GTGGTAT28700.022.412861
CATGGGA77500.022.0148984
GAGTACT104650.021.65115512-13
ACCGGTC1553.903298E-521.4553628
TGGTATC29400.021.1761932
AGTACTT109450.020.78843112-13
GTACTTT108450.020.74198214-15
Produced by FastQC (version 0.11.5)