FastQCFastQC Report
Thu 26 May 2016
SRR938485_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938485_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2250947
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142050.6310677239401905No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113250.5031215750526334No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92360.410316191363013No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41670.1851220841716842No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26070.11581792019092409No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24960.11088666236921615No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62000.049.93681
GTATCAA110700.041.458261
ATCAACG146250.031.1094933
TATCAAC147800.030.890732
TCAACGC147650.030.8145164
CAACGCA150200.030.2913675
AACGCAG155750.029.3358846
GTACATG134300.025.673991
TACATGG132100.025.6695422
ACGCAGA178100.025.601157
CGCAGAG179000.025.4193638
ACATGGG132850.024.6924633
GTGGTAT31350.023.5139471
GCAGAGT191250.023.4435169
GAGTACT111000.021.92921312-13
TGGTATC31950.021.7326852
AGTACTT115900.021.4938512-13
CATGGGG54300.021.4507794
GTACTTT115150.020.89140514-15
CATGGGA78000.020.4796034
Produced by FastQC (version 0.11.5)