FastQCFastQC Report
Thu 26 May 2016
SRR938484_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938484_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2246995
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139040.6187819732576174No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102510.4562092928555693No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78160.3478423405481543No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39700.176680410948845No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23720.10556320775079607No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23430.10427259517711432No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59750.051.501121
GTATCAA107100.042.362921
TATCAAC141000.031.9175662
ATCAACG143000.031.4711693
TCAACGC144100.031.230934
CAACGCA146950.030.5928865
AACGCAG152550.029.5885226
GTACATG129450.026.6827961
TACATGG129600.026.3646972
ACGCAGA172550.026.0172317
CGCAGAG173750.025.81028
ACATGGG133200.024.5104583
GTGGTAT30450.023.971711
GCAGAGT182200.023.698519
TGGTATC30900.022.9153612
GTGTAGC14300.022.3528881
GTACCGT1503.0285019E-522.1727056
GAGTACT108900.022.13624812-13
AGTACTT112550.021.777112-13
GTACTTT113200.021.2126414-15
Produced by FastQC (version 0.11.5)