##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938484_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2246995 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.021613755259803 31.0 31.0 34.0 28.0 34.0 2 31.192651964067565 31.0 31.0 34.0 28.0 34.0 3 31.227873671280978 31.0 31.0 34.0 27.0 34.0 4 34.911141324302015 37.0 35.0 37.0 32.0 37.0 5 34.90779730261972 37.0 35.0 37.0 32.0 37.0 6 34.879732709685605 37.0 35.0 37.0 32.0 37.0 7 34.798395635059265 37.0 35.0 37.0 32.0 37.0 8 34.76626605755687 37.0 35.0 37.0 32.0 37.0 9 36.216389889608124 39.0 35.0 39.0 32.0 39.0 10-11 36.04689507542295 38.0 35.0 39.0 30.0 39.0 12-13 35.85009089917868 38.0 35.0 39.0 30.0 39.0 14-15 36.9594965720885 39.0 36.0 41.0 31.0 41.0 16-17 36.89852247112255 39.0 36.0 41.0 31.0 41.0 18-19 36.929162058660566 39.0 36.0 41.0 31.0 41.0 20-21 36.88015660916024 39.0 36.0 41.0 30.5 41.0 22-23 36.712322679845755 39.0 36.0 41.0 30.0 41.0 24-25 36.565858624518526 39.0 36.0 41.0 30.0 41.0 26-27 36.34380962129421 39.0 35.5 41.0 29.5 41.0 28-29 36.092733628690766 39.0 35.0 40.0 28.5 41.0 30-31 35.79061301872056 38.0 35.0 40.0 27.0 41.0 32-33 35.470123876555135 38.0 34.0 40.0 27.0 41.0 34-35 35.144056840357905 38.0 34.0 40.0 26.0 41.0 36-37 34.89174274976135 38.0 34.0 40.0 25.0 41.0 38-39 34.62682070943638 38.0 33.5 40.0 24.5 41.0 40-41 34.32526307357159 38.0 33.0 40.0 24.0 41.0 42-43 33.894107908562326 38.0 33.0 40.0 22.5 41.0 44-45 33.37157492562289 37.5 32.5 40.0 20.0 41.0 46-47 32.82281647266683 37.0 31.0 40.0 16.5 41.0 48-49 32.290311949959836 37.0 30.5 39.5 11.5 41.0 50-51 31.57698481750071 35.5 30.0 38.5 9.0 40.5 52-53 31.59185289686893 36.0 30.0 39.0 8.5 40.0 54-55 31.56857269375321 36.0 30.0 39.0 7.0 40.0 56-57 31.147895077648148 36.0 29.5 39.0 2.0 40.0 58-59 30.691408970647466 35.0 28.5 39.0 2.0 40.0 60-61 30.15253371725349 35.0 28.0 39.0 2.0 40.0 62-63 29.419003825108646 34.5 26.5 38.0 2.0 40.0 64-65 28.758863726888578 34.0 26.0 38.0 2.0 40.0 66-67 28.060652115380762 34.0 25.5 37.5 2.0 39.5 68-69 27.19757854378848 33.0 22.5 36.5 2.0 39.0 70-71 26.362260485670863 32.0 20.5 36.0 2.0 39.0 72-73 25.442045042378822 31.5 15.5 35.5 2.0 38.5 74-75 24.49649376166836 31.0 7.5 35.0 2.0 38.0 76-77 23.5302014468212 30.0 4.0 34.5 2.0 37.0 78-79 22.52597157537066 29.0 2.0 34.0 2.0 36.0 80-81 21.530550134735503 28.5 2.0 34.0 2.0 36.0 82-83 20.50655453171903 26.5 2.0 33.5 2.0 35.0 84-85 19.502406324891687 26.0 2.0 33.0 2.0 35.0 86-87 18.476802351585118 24.5 2.0 32.0 2.0 35.0 88-89 17.3735346540602 21.0 2.0 32.0 2.0 34.0 90-91 16.21073589393835 13.0 2.0 31.0 2.0 34.0 92-93 15.032277552909552 2.0 2.0 31.0 2.0 34.0 94-95 13.769005494004215 2.0 2.0 30.0 2.0 34.0 96-97 12.371141012774839 2.0 2.0 29.0 2.0 34.0 98-99 10.731230376569595 2.0 2.0 26.5 2.0 33.5 100-101 7.585904062981895 2.0 2.0 2.0 2.0 28.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5262725199469251 1101 2 0.5908511305404183 1101 3 0.519444257862645 1101 4 0.5026667472192656 1101 5 0.5483371219979389 1101 6 0.6337581889530881 1101 7 0.4540091435198619 1101 8 0.5662190112464103 1101 9 0.5092325383158993 1101 10-11 0.7195312442277171 1101 12-13 0.6593535378808753 1101 14-15 0.7351992596539247 1101 16-17 0.7043475550231477 1101 18-19 0.5891629812878207 1101 20-21 0.985817514803287 1101 22-23 0.929544732678373 1101 24-25 0.7774605551519471 1101 26-27 1.0534619634297968 1101 28-29 1.150955316270391 1101 30-31 1.2009039616566142 1101 32-33 1.035681158236045 1101 34-35 1.1275384997403606 1101 36-37 1.1195562993308386 1101 38-39 1.2408245567113525 1101 40-41 1.2370370631654737 1101 42-43 1.5415337684218002 1101 44-45 1.4329659768369893 1101 46-47 1.5185087801193191 1101 48-49 1.5178523633660959 1101 50-51 1.5432841602347374 1101 52-53 1.5633918568647296 1101 54-55 1.6939926559961869 1101 56-57 2.0144635804191786 1101 58-59 2.018829573328233 1101 60-61 2.203095836412544 1101 62-63 2.062145282015166 1101 64-65 2.1195310711461737 1101 66-67 2.3450407907533446 1101 68-69 2.3430603189008927 1101 70-71 2.6185884572782747 1101 72-73 2.9163188484876983 1101 74-75 2.862944445903434 1101 76-77 3.064153917230641 1101 78-79 3.2503124181466667 1101 80-81 3.592526876620621 1101 82-83 3.651563204758798 1101 84-85 3.6042884086145115 1101 86-87 3.925009748173963 1101 88-89 4.301781138334576 1101 90-91 4.08056612837845 1101 92-93 4.044976480794029 1101 94-95 3.9973825004763004 1101 96-97 3.90629681056903 1101 98-99 3.293894701949595 1101 100-101 2.5783560340701763 1102 1 0.6532168789067967 1102 2 0.5345219849912937 1102 3 0.4239327972999334 1102 4 0.34320701387821373 1102 5 0.48721318690353144 1102 6 0.56489394348975 1102 7 0.5859752477317599 1102 8 0.6446006863009472 1102 9 0.6555279066916313 1102 10-11 0.7666756884594221 1102 12-13 0.710197803770221 1102 14-15 0.8833881437743685 1102 16-17 0.7887365515006692 1102 18-19 0.9137018199003677 1102 20-21 0.8494164345688446 1102 22-23 0.7309993506432662 1102 24-25 0.8474061091316898 1102 26-27 0.823539604696883 1102 28-29 0.751165834032939 1102 30-31 0.5591724577294883 1102 32-33 0.9176052336133651 1102 34-35 0.8781030870849165 1102 36-37 1.0166885085018151 1102 38-39 1.08556852405588 1102 40-41 1.0427208326102289 1102 42-43 1.5142839065332225 1102 44-45 1.254499987270414 1102 46-47 1.4059428357103911 1102 48-49 1.5047236061634024 1102 50-51 1.4771422157847134 1102 52-53 1.4000003260484881 1102 54-55 1.4385328768904024 1102 56-57 1.5975954477556336 1102 58-59 1.7386016182691932 1102 60-61 1.914380741943404 1102 62-63 1.9461870269216206 1102 64-65 2.1089653723724346 1102 66-67 2.4080164462513913 1102 68-69 2.516373223107376 1102 70-71 2.401008099314023 1102 72-73 2.756518681586524 1102 74-75 2.5209050340642847 1102 76-77 2.93001620648522 1102 78-79 3.2548849218557088 1102 80-81 3.4680717921356568 1102 82-83 3.50469811218095 1102 84-85 3.451010299613195 1102 86-87 3.611879052975013 1102 88-89 3.894118487912209 1102 90-91 3.6397050182661737 1102 92-93 3.627285516946765 1102 94-95 3.6272089867676574 1102 96-97 3.4641619932753773 1102 98-99 3.080073939985505 1102 100-101 1.9668898441661566 1103 1 0.7058787824142136 1103 2 0.668972298851088 1103 3 0.606712068927191 1103 4 0.47438133097198687 1103 5 0.4512190098884261 1103 6 0.48845000675225947 1103 7 0.6030163356579266 1103 8 0.5378645102124295 1103 9 0.6502268102263002 1103 10-11 0.6531256662030813 1103 12-13 0.7461304351961715 1103 14-15 0.8868886343222329 1103 16-17 0.8483728647609041 1103 18-19 0.946628255888406 1103 20-21 1.0037674351956483 1103 22-23 0.9721400788343217 1103 24-25 1.0474153069042345 1103 26-27 1.1499768418222658 1103 28-29 1.026928908496295 1103 30-31 0.9987710260564526 1103 32-33 1.0821706445380457 1103 34-35 1.2193942726664417 1103 36-37 1.329884246185884 1103 38-39 1.224485775897712 1103 40-41 1.3798515590752913 1103 42-43 1.4497827664636418 1103 44-45 1.5440167976191574 1103 46-47 1.7812487468122171 1103 48-49 1.696183531127751 1103 50-51 1.9507100885329614 1103 52-53 2.0536552483329906 1103 54-55 1.9843788902805422 1103 56-57 2.0590253266629794 1103 58-59 2.12249495590239 1103 60-61 2.3278803467798426 1103 62-63 2.3741884531441286 1103 64-65 2.416203792263243 1103 66-67 2.5561076245655414 1103 68-69 2.619186492427236 1103 70-71 2.5913546395889107 1103 72-73 2.8939607696852896 1103 74-75 2.891981559531896 1103 76-77 2.9392943743797737 1103 78-79 3.1260618279521317 1103 80-81 3.190306555627995 1103 82-83 3.4124987317247424 1103 84-85 3.63674750676066 1103 86-87 3.654424769311163 1103 88-89 3.9645487846936724 1103 90-91 3.9296298236367626 1103 92-93 3.960587812529111 1103 94-95 3.7980012897082194 1103 96-97 3.698491373459058 1103 98-99 3.819000616705374 1103 100-101 3.031582888379055 1104 1 0.5435555886937884 1104 2 0.5207734256019769 1104 3 0.39682444343445056 1104 4 0.3320792208904919 1104 5 0.3532575959534725 1104 6 0.5476046430058119 1104 7 0.5076470069850316 1104 8 0.4807336456544107 1104 9 0.5341298168992026 1104 10-11 0.574911806823657 1104 12-13 0.7023357237113927 1104 14-15 0.7484422322817181 1104 16-17 0.7032021345198345 1104 18-19 0.8095109604340607 1104 20-21 0.6281728207557151 1104 22-23 0.7693677520765689 1104 24-25 0.8493732727410119 1104 26-27 0.850030624424619 1104 28-29 0.9048174958495494 1104 30-31 0.862311789189711 1104 32-33 0.8537543221086565 1104 34-35 0.9267209445431916 1104 36-37 0.9509592970700211 1104 38-39 1.1856579575175843 1104 40-41 1.1551739326378652 1104 42-43 1.2284333276920734 1104 44-45 1.3774062394824185 1104 46-47 1.4374003600755856 1104 48-49 1.3044177441626772 1104 50-51 1.2181174021080743 1104 52-53 1.352295868904779 1104 54-55 1.4385035113332627 1104 56-57 1.8078108704147517 1104 58-59 1.925155852837321 1104 60-61 1.9689350440084823 1104 62-63 1.87798157584146 1104 64-65 2.217389693277493 1104 66-67 2.439580585120673 1104 68-69 2.5625587796800104 1104 70-71 2.6478421525783205 1104 72-73 2.822033677853373 1104 74-75 2.8259655084845825 1104 76-77 3.0136595035009606 1104 78-79 3.37251260559918 1104 80-81 3.4667012533719195 1104 82-83 3.536019656484921 1104 84-85 3.338822914048471 1104 86-87 3.36613305934646 1104 88-89 3.5648954194700835 1104 90-91 3.4490154996443536 1104 92-93 3.4865344292706357 1104 94-95 3.3837486853850027 1104 96-97 3.204282181679533 1104 98-99 2.9987415369997716 1104 100-101 2.185712662255953 1105 1 0.5502816437038653 1105 2 0.4245487035784379 1105 3 0.5055727365614437 1105 4 0.42969545081441396 1105 5 0.4644897957063847 1105 6 0.4253886341625872 1105 7 0.3940219338333364 1105 8 0.5271605914896398 1105 9 0.5776931175039266 1105 10-11 0.6329597943390937 1105 12-13 0.6192965143999913 1105 14-15 0.7716361980792286 1105 16-17 0.9421578533572088 1105 18-19 0.7695382704022009 1105 20-21 0.6531003192926832 1105 22-23 0.7010707236611964 1105 24-25 0.7738703141611225 1105 26-27 0.8020808227470013 1105 28-29 0.9343174633376847 1105 30-31 0.8366209120195478 1105 32-33 0.959874355920995 1105 34-35 1.0974732691416662 1105 36-37 1.1058328259080454 1105 38-39 1.2121967441747117 1105 40-41 1.3055881338212956 1105 42-43 1.3854058226527286 1105 44-45 1.491704958514866 1105 46-47 1.5825736483257984 1105 48-49 1.538858214934514 1105 50-51 1.3911299516886722 1105 52-53 1.5503932744578073 1105 54-55 1.7682882827748436 1105 56-57 1.9416466204635192 1105 58-59 2.0379174108059743 1105 60-61 2.101869565108686 1105 62-63 2.2635944245312345 1105 64-65 2.448402112810623 1105 66-67 2.4255094491560456 1105 68-69 2.6896918832628174 1105 70-71 2.6167491036154473 1105 72-73 2.8017033572863674 1105 74-75 2.9449641429861764 1105 76-77 2.992823298286055 1105 78-79 3.334953596560883 1105 80-81 3.5027231013464295 1105 82-83 3.6812111513805554 1105 84-85 3.6937861756389516 1105 86-87 3.6478717939846064 1105 88-89 3.591821747579843 1105 90-91 3.4348988470661617 1105 92-93 3.609580140955403 1105 94-95 3.6653053535688915 1105 96-97 3.747633504912516 1105 98-99 3.5667732685818 1105 100-101 2.31997496799755 1106 1 0.2792085479341999 1106 2 0.062030412722414496 1106 3 -0.05698460056053989 1106 4 0.034506453356286215 1106 5 0.002915380675503343 1106 6 -0.28247106989764603 1106 7 0.13905290989333707 1106 8 -0.10463022103995456 1106 9 0.30628213622487266 1106 10-11 0.1389780417029911 1106 12-13 0.19009087395424729 1106 14-15 0.30450803161069473 1106 16-17 -0.15047506341819172 1106 18-19 0.3861650209603482 1106 20-21 0.28351117492781697 1106 22-23 0.22632582915377242 1106 24-25 0.3380060289300886 1106 26-27 0.1658239485745696 1106 28-29 0.313623039028478 1106 30-31 -0.05558336567286659 1106 32-33 0.21754385440844004 1106 34-35 0.3814406210509702 1106 36-37 0.3819967235759094 1106 38-39 0.4423356290365561 1106 40-41 0.5558702527577708 1106 42-43 0.41851212707830143 1106 44-45 0.7181211105246845 1106 46-47 0.7313939485181322 1106 48-49 0.6111268609762419 1106 50-51 0.4043106759775732 1106 52-53 0.5847576611704817 1106 54-55 0.6072564562021192 1106 56-57 1.1314367951085735 1106 58-59 1.1246462228909913 1106 60-61 1.3436210414248038 1106 62-63 1.368764961997286 1106 64-65 1.3328312544693155 1106 66-67 1.4303293434218531 1106 68-69 1.6346560747441607 1106 70-71 1.6943476616097861 1106 72-73 1.558627593999521 1106 74-75 1.7835494890385384 1106 76-77 2.0728741357511424 1106 78-79 2.2309011933791894 1106 80-81 1.8923638563697693 1106 82-83 2.22395473145923 1106 84-85 2.246210716797002 1106 86-87 2.120012379871895 1106 88-89 1.9741895556732452 1106 90-91 2.037448935500155 1106 92-93 2.327126701694576 1106 94-95 2.13519304722967 1106 96-97 2.3097247997214847 1106 98-99 2.015694373861743 1106 100-101 1.4982698832845491 1107 1 0.2803665166723839 1107 2 0.19122642720388328 1107 3 0.19181738567287 1107 4 0.07724970094876227 1107 5 0.23057804135618198 1107 6 0.18731013593231438 1107 7 0.24881002938028018 1107 8 0.18742781162195143 1107 9 0.3366692678291514 1107 10-11 0.23709429390361692 1107 12-13 0.30282170758791693 1107 14-15 0.3244172702953776 1107 16-17 0.32317957194216973 1107 18-19 0.33096517402134396 1107 20-21 0.5381063419378265 1107 22-23 0.3651851168565372 1107 24-25 0.3335550981798079 1107 26-27 0.45902382072709713 1107 28-29 0.39154120923765134 1107 30-31 0.40160201017938846 1107 32-33 0.5573774598996479 1107 34-35 0.5768364439095635 1107 36-37 0.40213121554539555 1107 38-39 0.4649054519930331 1107 40-41 0.4465117296184147 1107 42-43 0.629072594329628 1107 44-45 0.5610886102762649 1107 46-47 0.3599833549766487 1107 48-49 0.3641645359322254 1107 50-51 0.6045035595310857 1107 52-53 0.7487374176670407 1107 54-55 0.7289030558600977 1107 56-57 0.8109395619234085 1107 58-59 0.7371299267891516 1107 60-61 1.0305975824539004 1107 62-63 1.0301176904727143 1107 64-65 0.970277657165088 1107 66-67 1.107354880284781 1107 68-69 1.223498194994928 1107 70-71 1.434398882167418 1107 72-73 1.4286703531909701 1107 74-75 1.7390288725230825 1107 76-77 1.7767804541150483 1107 78-79 2.1339295615739466 1107 80-81 2.229882265959219 1107 82-83 2.5575630035755808 1107 84-85 2.3248146581176883 1107 86-87 2.219825354757724 1107 88-89 2.3036993303680475 1107 90-91 2.2783670567654006 1107 92-93 2.1374109694860604 1107 94-95 2.0669125659180914 1107 96-97 2.3475603066099726 1107 98-99 2.412633487620772 1107 100-101 1.7772111298121258 1108 1 0.22571410115467216 1108 2 0.192309749871594 1108 3 0.09248362383930342 1108 4 0.23459660470638966 1108 5 0.17728672354918018 1108 6 0.21470262292611153 1108 7 0.13686844512021423 1108 8 0.12772516453127736 1108 9 0.3121361057083831 1108 10-11 0.300467790929666 1108 12-13 0.0962256403199433 1108 14-15 0.2264662304321945 1108 16-17 0.3988351210436534 1108 18-19 0.25943177139661344 1108 20-21 0.4011498844064718 1108 22-23 0.4242135441434627 1108 24-25 0.4421823690786866 1108 26-27 0.30742703616661515 1108 28-29 0.4267994767371235 1108 30-31 0.48333520689897114 1108 32-33 0.5546724935631673 1108 34-35 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-1.4460506724378632 2315 88-89 -1.4342008409427471 2315 90-91 -1.3507086010412834 2315 92-93 -1.1008227894475286 2315 94-95 -1.40950844374491 2315 96-97 -1.2281296208506127 2315 98-99 -1.27783601102748 2315 100-101 -0.5554004898779086 2316 1 -0.9089197979413051 2316 2 -0.675899690471244 2316 3 -0.6855860564140706 2316 4 -0.7517622715275536 2316 5 -0.5816891379002058 2316 6 -0.4406314711340471 2316 7 -0.58736617966089 2316 8 -0.5088275361383552 2316 9 -0.6332531388121012 2316 10-11 -0.6407581838242251 2316 12-13 -0.6926398129382676 2316 14-15 -0.8384389759428927 2316 16-17 -0.9648498495508093 2316 18-19 -1.0592664538124907 2316 20-21 -1.0087014721634446 2316 22-23 -1.1136854686994937 2316 24-25 -0.9994026267243541 2316 26-27 -1.1640959907931574 2316 28-29 -1.047076126994817 2316 30-31 -0.7808033839417376 2316 32-33 -1.0348256546793309 2316 34-35 -0.8504746832275032 2316 36-37 -1.1573495704737056 2316 38-39 -1.3127351486912602 2316 40-41 -1.3198731572991633 2316 42-43 -0.9182259394758177 2316 44-45 -0.9264542938083693 2316 46-47 -0.5379642301943903 2316 48-49 -0.4596088003653023 2316 50-51 -0.4833644124680738 2316 52-53 -0.6776224317009856 2316 54-55 -0.6307480586971899 2316 56-57 -0.5097796542264241 2316 58-59 -0.3485941965577197 2316 60-61 -0.48222551987064577 2316 62-63 -0.30357272503945154 2316 64-65 -0.3837393309546151 2316 66-67 -0.22886097945071526 2316 68-69 -0.27563999546938334 2316 70-71 -0.12593286875301857 2316 72-73 0.01117395903188978 2316 74-75 0.3887441330925334 2316 76-77 0.360152986282948 2316 78-79 0.2529721294749123 2316 80-81 0.0808278502952291 2316 82-83 0.16900619395855898 2316 84-85 0.2914728298207834 2316 86-87 0.3719640522086962 2316 88-89 0.3506898087790944 2316 90-91 0.260917362312977 2316 92-93 0.2579775474074708 2316 94-95 0.23232984033614024 2316 96-97 0.36251946300586546 2316 98-99 0.2239981899626695 2316 100-101 0.30639709089073275 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7560.0 3 2874.0 4 3067.0 5 3602.0 6 4423.0 7 5494.0 8 7191.0 9 9340.0 10 12011.0 11 15183.0 12 19261.0 13 22930.0 14 26684.0 15 30964.0 16 36155.0 17 41860.0 18 40996.0 19 38814.0 20 43089.0 21 46847.0 22 51973.0 23 57679.0 24 63765.0 25 70382.0 26 76440.0 27 85467.0 28 94047.0 29 102771.0 30 113839.0 31 126180.0 32 139741.0 33 155860.0 34 173358.0 35 183916.0 36 176692.0 37 125109.0 38 30963.0 39 468.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.748832853965794 19.342279144697518 14.60364390810082 22.305244093235867 2 15.177441038470786 20.577996598972568 41.07101202824099 23.173550334315653 3 19.00972732331664 25.878864774624372 29.931619365609347 25.17978853644964 4 12.47035399786384 16.699798360437505 37.65798030085211 33.17186734084655 5 13.179435798098545 38.433504775900076 33.90853427738072 14.478525148620665 6 31.033323522562473 36.598390528940065 17.86051577344867 14.50777017504879 7 27.79334011229812 33.803675955985405 20.764156134636654 17.63882779707982 8 26.475719912507717 35.24938995370282 19.757976013081993 18.51691412070747 9 27.271156959927843 16.66842517417015 20.275279880937912 35.785137984964095 10-11 24.894860408591384 27.421171183704963 27.120994951207173 20.562973456496483 12-13 25.80394071959689 24.734557219941227 27.857547006442925 21.603955054018957 14-15 22.864763356406705 25.865563085927633 26.731643670084082 24.538029887581576 16-17 21.539279891833544 29.25226042298426 27.23390996144066 21.974549723741536 18-19 21.407201022421788 27.462962361185365 30.26360480246222 20.86623181393063 20-21 22.53417857890121 26.588686233745417 30.271202738738097 20.60593244861527 22-23 21.95845578439798 26.546144324536446 30.18264879821556 21.312751092850014 24-25 21.514898417754065 26.79304362072934 30.414837022747314 21.27722093876928 26-27 21.34881732072052 27.118072489760088 30.314644352582064 21.218465836937327 28-29 21.223870285054673 27.273819958768502 30.350801592456357 21.151508163720468 30-31 21.62212997072932 27.033733625669164 30.263480651300483 21.08065575230103 32-33 21.242213905183533 27.048300775576838 30.18354984714381 21.525935472095817 34-35 21.583800124913026 27.25263014036467 30.128167175118183 21.035402559604126 36-37 21.656530438400125 27.062449598019512 30.029864460268378 21.25115550331198 38-39 21.46194276598647 27.25724994809027 30.05376579797333 21.227041487949933 40-41 21.58350135530304 27.154976343396847 30.059197144294153 21.202325157005962 42-43 21.407214912929796 27.182563570963293 29.807343722842372 21.60287779326454 44-45 21.47741533323141 27.381183669009403 29.734710532107822 21.40669046565137 46-47 21.53259848183711 27.403561312975427 29.301353954163105 21.762486251024356 48-49 21.534007272345278 27.580184750864976 29.202930837939938 21.682877138849804 50-51 21.634126317724455 27.669960277711077 28.967427456026517 21.728485948537948 52-53 21.63968354641653 27.81776101300753 28.852177009268843 21.690378431307096 54-55 21.40438806456408 27.71136298291542 28.97379053614394 21.910458416376553 56-57 21.59830246707525 27.988107477779412 28.585893652722326 21.827696402423015 58-59 21.654804797086484 27.605900648692167 28.90608884085245 21.833205713368898 60-61 21.80630410802032 27.556967318271713 29.034088595231562 21.602639978476404 62-63 21.61735715999318 27.561270238885278 28.96593578124979 21.855436819871752 64-65 21.474146993659478 27.556326324606623 29.016057375363737 21.953469306370163 66-67 21.567783588488073 27.772831466579913 28.636308307718462 22.023076637213553 68-69 21.55917285382521 27.50692300091951 28.766481904953494 22.16742224030179 70-71 21.505777646030133 27.41370251159524 28.75501369886183 22.325506143512797 72-73 21.55587013081232 27.573624073864305 28.506786524735418 22.36371927058796 74-75 21.334294127059334 27.660622315710203 28.451875116696158 22.55320844053431 76-77 21.35568764521561 27.48046230441442 28.552618314908106 22.61123173546186 78-79 21.462151148830184 27.465817275391885 28.365992214210035 22.706039361567893 80-81 21.404913230542437 27.551670105368714 28.232036833984687 22.81137983010416 82-83 21.58362209210459 27.489056098206916 28.141862343853276 22.78545946583522 84-85 21.61683224237774 27.197529649854214 28.05183088712114 23.1338072206469 86-87 21.391436740646654 27.137504775947164 28.283776599828254 23.187281883577928 88-89 21.592964667550945 27.01701637096846 28.216018445158593 23.174000516322007 90-91 21.516268017768375 27.017627265617104 28.157218703357234 23.308886013257286 92-93 21.699708581328508 26.789443055411834 28.043110824773816 23.467737538485846 94-95 21.43761381274418 26.807349715988376 27.977626267324794 23.777410203942644 96-97 21.513264752449597 26.54114474673428 27.86203375511274 24.083556745703383 98-99 21.405800497650944 26.478048011899823 27.63295783631107 24.483193654138155 100-101 22.28344488423169 25.734504851695455 27.73326435758312 24.248785906489736 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2621.0 1 1893.5 2 1438.5 3 1690.5 4 1377.0 5 1204.5 6 2077.5 7 4330.5 8 6545.0 9 6874.5 10 5380.0 11 4028.0 12 3728.0 13 3729.0 14 3787.0 15 3853.5 16 3940.5 17 4113.0 18 4311.0 19 4590.5 20 5113.5 21 5833.5 22 6673.0 23 7851.0 24 9425.0 25 11503.0 26 14387.0 27 17765.0 28 20638.5 29 23650.0 30 27288.5 31 31166.5 32 35854.5 33 40745.5 34 45640.5 35 50802.5 36 56659.5 37 62038.0 38 65250.5 39 66977.5 40 68891.5 41 71231.5 42 73055.0 43 75294.5 44 77628.0 45 79494.5 46 80271.5 47 80618.0 48 81165.0 49 80815.0 50 79335.0 51 76622.0 52 73714.5 53 70936.0 54 67817.5 55 63235.0 56 57741.0 57 52129.0 58 46708.0 59 41834.5 60 36672.5 61 30844.5 62 25016.5 63 19392.5 64 14599.0 65 11250.5 66 8481.0 67 6403.0 68 4914.5 69 3668.0 70 2799.0 71 2138.0 72 1694.5 73 1404.0 74 1114.0 75 871.5 76 605.0 77 399.5 78 277.5 79 173.0 80 111.0 81 57.5 82 30.0 83 23.0 84 14.0 85 9.0 86 6.0 87 5.5 88 5.0 89 4.0 90 2.5 91 2.0 92 1.5 93 1.0 94 0.5 95 0.0 96 1.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3840239964930941 2 0.027369887338423094 3 0.03315539197906538 4 0.04040952472079377 5 0.059857721089722045 6 0.05180251847467396 7 0.036982725818259496 8 0.03800631510083467 9 0.03435699678904493 10-11 0.03624841176771644 12-13 0.029105538730615776 14-15 0.027169619870093167 16-17 0.020360525946875718 18-19 0.025100189364017277 20-21 0.0313752367050216 22-23 0.03068542653632963 24-25 0.039341431556367504 26-27 0.03384520214775734 28-29 0.029105538730615776 30-31 0.03204279493278801 32-33 0.026813588815284416 34-35 0.0281264533298917 36-37 0.030240387717818687 38-39 0.013640439787360452 40-41 0.011793528690540032 42-43 0.012171811686274335 44-45 0.011593261222210107 46-47 0.020649801178907832 48-49 0.017890560504139976 50-51 0.012082803922572147 52-53 0.00979085400724078 54-55 0.007699171560239342 56-57 0.009123295779474365 58-59 0.003983097425672954 60-61 0.007053865273398473 62-63 0.011927040336093316 64-65 0.00787718708764372 66-67 0.004027601307524048 68-69 0.006097031813599941 70-71 0.012260819449976524 72-73 0.010080129239272895 74-75 0.010747687467039312 76-77 0.005362717763056882 78-79 0.00932356324780429 80-81 0.01181578063146558 82-83 0.013796203373839282 84-85 0.008366729788005758 86-87 0.0026034770882890257 88-89 0.014908800420116646 90-91 0.023676065144782252 92-93 0.01012463312112399 94-95 0.019225676959672807 96-97 0.017890560504139976 98-99 0.010658679703337124 100-101 0.12140658968978568 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2246995.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.312684645877916 #Duplication Level Percentage of deduplicated Percentage of total 1 74.61072015926302 39.77697795052635 2 13.652830711476144 14.557381164889694 3 4.918510988777083 7.866570758158733 4 2.308090746451543 4.922020563985604 5 1.2214880619494488 3.2560403922707772 6 0.7462559634708161 2.387094530737525 7 0.4937181927385563 1.8425009619382395 8 0.3480597229130824 1.4844798596477449 9 0.23490940430215182 1.127128589272049 >10 1.3202540215828482 12.916561740155021 >50 0.10236053788652169 3.763714534884799 >100 0.041121205079515916 3.752297033514071 >500 8.401420545824779E-4 0.27550469497263513 >1k 5.880994382076478E-4 0.6397388324327198 >5k 8.40142054582354E-5 0.35008042528139316 >10k+ 1.680284109164708E-4 1.0819079673326575 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13904 0.6187819732576174 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10251 0.4562092928555693 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7816 0.3478423405481543 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3970 0.176680410948845 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2372 0.10556320775079607 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2343 0.10427259517711432 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 4.4503881851094464E-5 6 0.0 0.0 0.0 0.0 4.4503881851094464E-5 7 0.0 0.0 0.0 0.0 4.4503881851094464E-5 8 0.0 0.0 0.0 0.0 4.4503881851094464E-5 9 0.0 0.0 0.0 0.0 4.4503881851094464E-5 10-11 0.0 0.0 0.0 2.2251940925547232E-5 4.4503881851094464E-5 12-13 0.0 0.0 0.0 4.4503881851094464E-5 4.4503881851094464E-5 14-15 0.0 0.0 0.0 6.675582277664169E-5 4.4503881851094464E-5 16-17 0.0 0.0 0.0 1.3351164555328339E-4 4.4503881851094464E-5 18-19 0.0 0.0 0.0 1.3351164555328339E-4 4.4503881851094464E-5 20-21 0.0 0.0 0.0 2.6702329110656677E-4 6.675582277664169E-5 22-23 0.0 0.0 0.0 6.230543459153225E-4 8.900776370218893E-5 24-25 0.0 0.0 0.0 0.0017801552740437784 8.900776370218893E-5 26-27 0.0 0.0 0.0 0.003115271729576612 1.3351164555328339E-4 28-29 0.0 0.0 0.0 0.007120621096175114 1.3351164555328339E-4 30-31 0.0 0.0 0.0 0.01806857603154435 1.3351164555328339E-4 32-33 0.0 0.0 0.0 0.038117574805462406 1.7801552740437786E-4 34-35 0.0 0.0 0.0 0.06791292370477015 1.7801552740437786E-4 36-37 0.0 0.0 0.0 0.12040525234813607 1.7801552740437786E-4 38-39 0.0 0.0 0.0 0.20200311972211776 1.7801552740437786E-4 40-41 0.0 0.0 0.0 0.2897425227915505 1.7801552740437786E-4 42-43 0.0 0.0 0.0 0.3764360846374825 1.7801552740437786E-4 44-45 0.0 0.0 0.0 0.4842467384217588 2.002674683299251E-4 46-47 0.0 0.0 0.0 0.6111940614020058 2.225194092554723E-4 48-49 0.0 0.0 0.0 0.7490225834948454 2.225194092554723E-4 50-51 0.0 0.0 0.0 0.9001132623793111 2.225194092554723E-4 52-53 0.0 0.0 0.0 1.051048177677298 2.225194092554723E-4 54-55 0.0 0.0 0.0 1.213954637193229 2.225194092554723E-4 56-57 2.2251940925547232E-5 0.0 0.0 1.4038527010518493 2.225194092554723E-4 58-59 4.4503881851094464E-5 0.0 0.0 1.6135327403932807 2.225194092554723E-4 60-61 4.4503881851094464E-5 0.0 0.0 1.837342762222435 2.225194092554723E-4 62-63 4.4503881851094464E-5 0.0 0.0 2.062465648566196 2.6702329110656677E-4 64-65 4.4503881851094464E-5 0.0 0.0 2.2920166711541414 2.6702329110656677E-4 66-67 4.4503881851094464E-5 0.0 0.0 2.553209953738215 2.6702329110656677E-4 68-69 4.4503881851094464E-5 0.0 0.0 2.8474251166558 2.89275232032114E-4 70-71 6.675582277664169E-5 0.0 0.0 3.176175291889835 3.1152717295766124E-4 72-73 8.900776370218893E-5 0.0 0.0 3.4932877020198085 3.1152717295766124E-4 74-75 8.900776370218893E-5 0.0 0.0 3.8082861777618553 3.1152717295766124E-4 76-77 8.900776370218893E-5 0.0 0.0 4.150543281137697 3.1152717295766124E-4 78-79 8.900776370218893E-5 0.0 0.0 4.530517424382342 3.1152717295766124E-4 80-81 8.900776370218893E-5 0.0 0.0 4.943224172728466 3.1152717295766124E-4 82-83 8.900776370218893E-5 0.0 0.0 5.355508134197005 3.1152717295766124E-4 84-85 8.900776370218893E-5 0.0 0.0 5.76436529676301 3.1152717295766124E-4 86-87 8.900776370218893E-5 0.0 0.0 6.188242519453759 3.337791138832085E-4 88-89 8.900776370218893E-5 0.0 0.0 6.629543011889212 3.560310548087557E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5975 0.0 51.50112 1 GTATCAA 10710 0.0 42.36292 1 TATCAAC 14100 0.0 31.917566 2 ATCAACG 14300 0.0 31.471169 3 TCAACGC 14410 0.0 31.23093 4 CAACGCA 14695 0.0 30.592886 5 AACGCAG 15255 0.0 29.588522 6 GTACATG 12945 0.0 26.682796 1 TACATGG 12960 0.0 26.364697 2 ACGCAGA 17255 0.0 26.017231 7 CGCAGAG 17375 0.0 25.8102 8 ACATGGG 13320 0.0 24.510458 3 GTGGTAT 3045 0.0 23.97171 1 GCAGAGT 18220 0.0 23.69851 9 TGGTATC 3090 0.0 22.915361 2 GTGTAGC 1430 0.0 22.352888 1 GTACCGT 150 3.0285019E-5 22.172705 6 GAGTACT 10890 0.0 22.136248 12-13 AGTACTT 11255 0.0 21.7771 12-13 GTACTTT 11320 0.0 21.21264 14-15 >>END_MODULE