FastQCFastQC Report
Thu 26 May 2016
SRR938484_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938484_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2246995
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138060.6144205928362101No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111590.4966188175763631No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87200.3880738497415437No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40720.18121980689765665No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26370.1173567364413361No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24630.10961306099924566No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63300.052.844591
GTATCAA111500.040.2706381
ATCAACG147400.030.2267863
TATCAAC148050.030.130182
TCAACGC148600.030.0139884
CAACGCA151100.029.4859625
AACGCAG156250.028.4837066
ACGCAGA180550.024.6238127
CGCAGAG181200.024.4830558
GTACATG134850.024.3125061
TACATGG134450.023.9912872
ACATGGG135000.023.081113
GCAGAGT193500.022.4603779
GTGGTAT30350.021.6048851
GAGTACT109350.021.47951312-13
AGTACTT113650.021.39820912-13
TGGTATC29800.021.3615682
CATGGGA79850.020.6410354
GTACTTT112650.020.5129714-15
AGAGTAC185850.019.94744510-11
Produced by FastQC (version 0.11.5)