##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938484_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2246995 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.798106359827237 31.0 31.0 34.0 30.0 34.0 2 32.037366349279814 31.0 31.0 34.0 30.0 34.0 3 32.08741853008129 33.0 31.0 34.0 30.0 34.0 4 35.75648009897663 37.0 35.0 37.0 35.0 37.0 5 35.65658935600658 37.0 35.0 37.0 33.0 37.0 6 35.691806612831805 37.0 35.0 37.0 33.0 37.0 7 35.61151938477834 37.0 35.0 37.0 33.0 37.0 8 35.58922249493212 37.0 35.0 37.0 33.0 37.0 9 37.11779732487166 39.0 37.0 39.0 33.0 39.0 10-11 37.05055908001576 39.0 37.0 39.0 33.0 39.0 12-13 36.92351740880598 39.0 37.0 39.0 33.0 39.0 14-15 37.891810395661764 40.0 37.0 41.0 32.5 41.0 16-17 37.988542253098025 40.0 37.0 41.0 33.0 41.0 18-19 38.15817680947221 40.0 37.0 41.0 33.0 41.0 20-21 38.13254190596775 40.0 37.0 41.0 33.0 41.0 22-23 37.995095227181196 40.0 37.0 41.0 33.0 41.0 24-25 37.87739225053905 40.0 37.0 41.0 32.5 41.0 26-27 37.65990022229689 40.0 37.0 41.0 32.0 41.0 28-29 37.50896664211536 39.5 36.5 41.0 32.0 41.0 30-31 37.223898807073454 39.0 36.0 41.0 31.0 41.0 32-33 37.077866884439 39.0 36.0 41.0 31.0 41.0 34-35 36.92754567767173 39.0 35.0 41.0 31.0 41.0 36-37 36.79105672242261 39.0 35.0 41.0 30.0 41.0 38-39 36.572782760976324 39.0 35.0 40.0 30.0 41.0 40-41 36.33969545993649 39.0 35.0 40.0 30.0 41.0 42-43 36.061209526500946 38.0 35.0 40.0 30.0 41.0 44-45 35.72384384477937 38.0 35.0 40.0 28.5 41.0 46-47 35.25356954510357 38.0 34.0 40.0 26.5 41.0 48-49 34.811914134210355 38.0 34.0 40.0 25.5 41.0 50-51 34.42805413452189 38.0 33.0 40.0 24.0 41.0 52-53 33.84974977692429 37.5 33.0 40.0 23.0 41.0 54-55 33.44645915990022 37.0 32.5 40.0 22.0 41.0 56-57 33.08182505969083 37.0 32.0 39.5 20.5 40.5 58-59 32.56702840905298 36.0 31.0 39.0 18.5 40.0 60-61 32.100368047102904 36.0 31.0 39.0 17.5 40.0 62-63 31.612333360777395 35.0 30.0 39.0 12.5 40.0 64-65 30.983120790210926 35.0 29.0 38.0 8.0 40.0 66-67 30.356939601556746 34.0 28.5 38.0 7.0 40.0 68-69 29.654476311696286 34.0 28.0 37.0 2.0 39.0 70-71 28.909263705526712 33.5 26.0 36.5 2.0 39.0 72-73 28.341150736873026 33.0 26.0 36.0 2.0 39.0 74-75 27.62821946644296 32.5 25.5 35.5 2.0 38.0 76-77 26.467470110080352 31.0 24.0 34.0 2.0 36.0 78-79 26.98624028980928 32.0 25.0 35.0 2.0 37.0 80-81 26.605309980663066 32.0 24.5 35.0 2.0 36.5 82-83 25.925169392900294 31.5 22.0 35.0 2.0 36.0 84-85 25.150627170954987 31.0 19.5 34.0 2.0 35.5 86-87 24.238053711735006 30.5 15.0 34.0 2.0 35.0 88-89 23.268494144401746 30.0 7.0 34.0 2.0 35.0 90-91 22.414526289555607 29.0 2.0 34.0 2.0 35.0 92-93 21.335940667424715 29.0 2.0 34.0 2.0 35.0 94-95 20.154467855958735 27.0 2.0 33.0 2.0 35.0 96-97 18.775462339702578 25.5 2.0 32.5 2.0 34.0 98-99 17.022615982679092 19.0 2.0 32.0 2.0 34.0 100-101 12.80536783570947 2.0 2.0 27.5 2.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3692200877604854 1101 2 0.2898382756113378 1101 3 0.2790486128532663 1101 4 0.16849516042488233 1101 5 0.16533131277037683 1101 6 0.19152980821463927 1101 7 0.2988673914447446 1101 8 0.26650352297235713 1101 9 0.3790659092236126 1101 10-11 0.3494825060793829 1101 12-13 0.4307360410741907 1101 14-15 0.5126241569461669 1101 16-17 0.37918163756357615 1101 18-19 0.39744169452010425 1101 20-21 0.4034000005501994 1101 22-23 0.3845926078993358 1101 24-25 0.35801645416103156 1101 26-27 0.5515848393114027 1101 28-29 0.5558629652016194 1101 30-31 0.617156122371135 1101 32-33 0.39512533156720764 1101 34-35 0.5055158942693723 1101 36-37 0.5862446843793094 1101 38-39 0.6673162159370989 1101 40-41 0.6229788878318985 1101 42-43 0.6998530134304346 1101 44-45 0.7766910281921184 1101 46-47 0.8686620373686935 1101 48-49 0.6805441992393781 1101 50-51 0.8157634337289963 1101 52-53 0.8665620152124305 1101 54-55 0.9068380795259117 1101 56-57 0.9561622106912324 1101 58-59 1.0923893963047178 1101 60-61 1.1327324157022574 1101 62-63 1.3902692109381043 1101 64-65 1.3302294210643169 1101 66-67 1.3956204527328104 1101 68-69 1.3842573374805376 1101 70-71 1.7048510738267701 1101 72-73 1.7485618100876223 1101 74-75 1.6729004411845452 1101 76-77 1.6704856935203551 1101 78-79 1.6823756111963775 1101 80-81 1.914645743526954 1101 82-83 1.9856192391119691 1101 84-85 2.2412749583022737 1101 86-87 2.253378262490724 1101 88-89 2.0650850312048696 1101 90-91 2.5313967654159235 1101 92-93 2.832108587303992 1101 94-95 2.4795877500503423 1101 96-97 2.379987709173893 1101 98-99 2.4594021185465564 1101 100-101 2.2690726888349424 1102 1 0.22781477428842223 1102 2 0.16948053551111286 1102 3 0.18723195383740432 1102 4 0.16515129964679431 1102 5 0.06544628498230765 1102 6 0.20631181632433737 1102 7 0.17427874866321957 1102 8 0.19093497791254066 1102 9 0.2651437176720606 1102 10-11 0.23868387416464998 1102 12-13 0.20641974514075656 1102 14-15 0.32090285668610363 1102 16-17 0.23757026605528608 1102 18-19 0.2864939769893695 1102 20-21 0.18929215829655988 1102 22-23 0.3331558521891367 1102 24-25 0.2943691545370015 1102 26-27 0.37763426428689684 1102 28-29 0.22917400145989575 1102 30-31 0.21197936076755752 1102 32-33 0.2593044827435236 1102 34-35 0.4695874282798016 1102 36-37 0.46479874770368923 1102 38-39 0.5740816674878886 1102 40-41 0.6034157495481551 1102 42-43 0.6340574566254347 1102 44-45 0.5381896503468298 1102 46-47 0.3457572564953608 1102 48-49 0.34331568799876067 1102 50-51 0.4533714419965307 1102 52-53 0.4420078594246135 1102 54-55 0.4174812981501006 1102 56-57 0.6738904868038631 1102 58-59 0.6124039943199335 1102 60-61 0.4371991001455626 1102 62-63 0.6206653280341676 1102 64-65 0.9346268279786187 1102 66-67 0.938522786593996 1102 68-69 0.8820407834433652 1102 70-71 1.0871872652765155 1102 72-73 1.218080631300225 1102 74-75 1.1493300870168994 1102 76-77 1.209635436187142 1102 78-79 1.5041278213293445 1102 80-81 1.6956681603063615 1102 82-83 1.6711509762524308 1102 84-85 1.7027326490419696 1102 86-87 1.6623676231247444 1102 88-89 1.5776375263867308 1102 90-91 1.8578047196997574 1102 92-93 2.034541870031493 1102 94-95 1.9580695704779387 1102 96-97 1.9757549618937418 1102 98-99 1.6944606343589719 1102 100-101 1.8158848717799199 1103 1 0.340712776668493 1103 2 0.29112709577128726 1103 3 0.2573257938279596 1103 4 0.2340982568873713 1103 5 0.21571654052141298 1103 6 0.24510620409333939 1103 7 0.3157323146737028 1103 8 0.25655256859576525 1103 9 0.2934762637063457 1103 10-11 0.419436342801383 1103 12-13 0.5102647547246093 1103 14-15 0.5984764161559468 1103 16-17 0.44061699982405855 1103 18-19 0.4275697530088465 1103 20-21 0.44009134253362703 1103 22-23 0.5671120980444542 1103 24-25 0.5215593599312172 1103 26-27 0.5554801232452959 1103 28-29 0.5818091065188966 1103 30-31 0.47209248201463794 1103 32-33 0.5684965776554591 1103 34-35 0.6281052469769506 1103 36-37 0.5698734199904578 1103 38-39 0.5719830991609243 1103 40-41 0.5217621263070882 1103 42-43 0.47316661261056936 1103 44-45 0.5161153368867417 1103 46-47 0.4802108633848263 1103 48-49 0.7417684182610245 1103 50-51 0.824602626306266 1103 52-53 0.9289412312144734 1103 54-55 0.7158678271605581 1103 56-57 0.983044427992624 1103 58-59 1.071684319716688 1103 60-61 1.1834363027509127 1103 62-63 1.3014138223247365 1103 64-65 1.2785576607290494 1103 66-67 1.3453617279190233 1103 68-69 1.1348070419257539 1103 70-71 1.448342005876622 1103 72-73 1.5025415055655422 1103 74-75 1.2553398661795114 1103 76-77 1.2346590551586267 1103 78-79 1.220102081192536 1103 80-81 1.3963799089278304 1103 82-83 1.561193649374946 1103 84-85 1.6627832027251657 1103 86-87 1.7802119831868808 1103 88-89 1.6051526061916022 1103 90-91 2.055062193741602 1103 92-93 2.425342167085482 1103 94-95 2.216861051296611 1103 96-97 2.1492863842100363 1103 98-99 2.490897689799965 1103 100-101 2.303987042000033 1104 1 0.17973919348043665 1104 2 0.18795059436713757 1104 3 0.16374191873241273 1104 4 0.1527888383835787 1104 5 0.11883571675658544 1104 6 0.09927677116164091 1104 7 0.08269283545246253 1104 8 0.13741712661227012 1104 9 0.09206202817075138 1104 10-11 0.22606019093047536 1104 12-13 0.15370166698060217 1104 14-15 0.26235275696374316 1104 16-17 0.25440177065220837 1104 18-19 0.2538615016197312 1104 20-21 0.35386977968205 1104 22-23 0.2739839258370793 1104 24-25 0.25958269363669473 1104 26-27 0.502582518449735 1104 28-29 0.36250984766677874 1104 30-31 0.42417969785785203 1104 32-33 0.5565276405972028 1104 34-35 0.4403766977545942 1104 36-37 0.47168442298768554 1104 38-39 0.5394903560549764 1104 40-41 0.6968551991937844 1104 42-43 0.5531000123580796 1104 44-45 0.7349039293928143 1104 46-47 0.8635828071159892 1104 48-49 0.9671565976823686 1104 50-51 0.7869998918742382 1104 52-53 0.8487236746837041 1104 54-55 1.0094569624307752 1104 56-57 0.9980350670287024 1104 58-59 0.9993684043604176 1104 60-61 1.0835270139265347 1104 62-63 1.158268581387265 1104 64-65 1.3463881499897 1104 66-67 1.3778568327360539 1104 68-69 1.4154662384287064 1104 70-71 1.5316651051285994 1104 72-73 1.7220325997677577 1104 74-75 1.8094044783006709 1104 76-77 1.6287588567748976 1104 78-79 1.748836268463343 1104 80-81 1.9301905941675663 1104 82-83 2.0043469416001933 1104 84-85 2.2852115140588047 1104 86-87 2.155170760593464 1104 88-89 2.2754372353170815 1104 90-91 2.4064736381640977 1104 92-93 2.4350149842203415 1104 94-95 2.3429289741073056 1104 96-97 2.3295449486332203 1104 98-99 2.6582864317379418 1104 100-101 2.025131011907538 1105 1 0.19017680320798647 1105 2 0.09170246379948566 1105 3 0.12872533768032923 1105 4 0.0994745786787874 1105 5 0.07348556395081118 1105 6 0.10720966666850273 1105 7 0.1966508301205181 1105 8 0.17728447819560245 1105 9 0.19526249634163406 1105 10-11 0.24749201354573103 1105 12-13 0.2715051652574729 1105 14-15 0.3646623999834375 1105 16-17 0.327576879566962 1105 18-19 0.2445514034338956 1105 20-21 0.29022715012225575 1105 22-23 0.2760893943330771 1105 24-25 0.26637897427913515 1105 26-27 0.25277433846404307 1105 28-29 0.26875602752242145 1105 30-31 0.47353401218258284 1105 32-33 0.4837764864259313 1105 34-35 0.5164817761082006 1105 36-37 0.5950123376765433 1105 38-39 0.6100398066044264 1105 40-41 0.6606564866637115 1105 42-43 0.5756619474643969 1105 44-45 0.5946178249647005 1105 46-47 0.6605566025513241 1105 48-49 0.811350628503611 1105 50-51 0.7514241067126548 1105 52-53 0.8268496906795377 1105 54-55 0.8725104769635834 1105 56-57 0.9581169969331143 1105 58-59 1.0800810644813552 1105 60-61 1.0499006403001587 1105 62-63 1.0522317779540096 1105 64-65 1.2076951270363239 1105 66-67 1.3605592191070777 1105 68-69 1.6440051551333035 1105 70-71 1.566255130487857 1105 72-73 1.6579712173631762 1105 74-75 1.5635510417963516 1105 76-77 1.645852295880168 1105 78-79 1.77858072519005 1105 80-81 1.674209060907927 1105 82-83 1.9481079143552513 1105 84-85 2.244376609318575 1105 86-87 2.2786911455339656 1105 88-89 2.290586464785836 1105 90-91 2.3935859341521315 1105 92-93 2.5916012382030438 1105 94-95 2.3353485072368976 1105 96-97 2.341658870214598 1105 98-99 2.3566218241561785 1105 100-101 2.2668970565138213 1106 1 -0.02530214176963952 1106 2 -0.01729750720576817 1106 3 0.019767505714582967 1106 4 0.030811719989543462 1106 5 0.07029246856774307 1106 6 -0.0014964908503216634 1106 7 0.02726464901929404 1106 8 -0.009270268728542419 1106 9 -0.10582635324325906 1106 10-11 -0.05778213183395309 1106 12-13 0.026430890367890925 1106 14-15 -0.0015356148425453853 1106 16-17 -0.10983582206737452 1106 18-19 -0.032093420519636595 1106 20-21 0.0021592637430316586 1106 22-23 0.06117314084162473 1106 24-25 -0.09041314301430248 1106 26-27 -0.22021560035702947 1106 28-29 -0.05785709550230678 1106 30-31 0.07410269591748886 1106 32-33 -0.17032994364355858 1106 34-35 -0.2399155293128601 1106 36-37 -0.2053871133561529 1106 38-39 -0.06314140093034126 1106 40-41 -0.17091203276760325 1106 42-43 -0.12325935111389441 1106 44-45 0.06464527332988013 1106 46-47 -0.515815380786357 1106 48-49 -0.20068731596177258 1106 50-51 0.07692170498069828 1106 52-53 -0.22540175180937183 1106 54-55 -0.15109318410709704 1106 56-57 0.029380781521439303 1106 58-59 0.19756113058939206 1106 60-61 0.30722635767865114 1106 62-63 0.1138675652098513 1106 64-65 0.19084753311749125 1106 66-67 0.31096572552941737 1106 68-69 0.2122989106302242 1106 70-71 0.010400800121654896 1106 72-73 0.12740688284105772 1106 74-75 0.2689985757926969 1106 76-77 0.2870664994426555 1106 78-79 0.45196576583102654 1106 80-81 0.5894600104860537 1106 82-83 0.8510861586263694 1106 84-85 0.8624094120479455 1106 86-87 0.9458095504360138 1106 88-89 0.941445772584462 1106 90-91 1.285537957502573 1106 92-93 1.1760028348473845 1106 94-95 0.9050503445376776 1106 96-97 0.7596892567146405 1106 98-99 0.8193964286194344 1106 100-101 0.7977014675820833 1107 1 0.023193129209136742 1107 2 0.01960954828329875 1107 3 0.10288244532816293 1107 4 0.10685908287610602 1107 5 0.03315107550502461 1107 6 0.04700174642685795 1107 7 -0.052562890391683936 1107 8 0.0010083260172564223 1107 9 0.13226766655085243 1107 10-11 0.1698193489422053 1107 12-13 0.11298636533901174 1107 14-15 0.1714607618239654 1107 16-17 0.18428805203699028 1107 18-19 0.0956314953487194 1107 20-21 0.09247767362911219 1107 22-23 0.03527883895254291 1107 24-25 0.07940243479837505 1107 26-27 0.14639774709588238 1107 28-29 0.0681529291031353 1107 30-31 0.11930166779846729 1107 32-33 0.0678777224580287 1107 34-35 0.17335565265323538 1107 36-37 0.11377713948519386 1107 38-39 0.22237724151353433 1107 40-41 0.27078333283608913 1107 42-43 0.24352122141136334 1107 44-45 0.2892997366449279 1107 46-47 0.3143421593217042 1107 48-49 0.4704171185060275 1107 50-51 0.34792105832764975 1107 52-53 0.2821695394648174 1107 54-55 0.45301146838363593 1107 56-57 0.4432674704056865 1107 58-59 0.35810887983632256 1107 60-61 0.42852960829674913 1107 62-63 0.7082188431653584 1107 64-65 0.7210629902018262 1107 66-67 0.6104469199391325 1107 68-69 0.751256419888783 1107 70-71 0.6247443731170641 1107 72-73 0.8990419510140129 1107 74-75 1.0331099988595227 1107 76-77 1.1276201738706426 1107 78-79 1.0456863345890817 1107 80-81 1.2573777135032245 1107 82-83 1.426760880276344 1107 84-85 1.4781710610725511 1107 86-87 1.7084367119405819 1107 88-89 1.584117495306753 1107 90-91 1.9538753917434448 1107 92-93 2.0519151569992538 1107 94-95 1.7992220872824838 1107 96-97 1.8335194969403155 1107 98-99 1.9898862831373307 1107 100-101 1.4975988336574186 1108 1 -0.0033628681298480956 1108 2 0.0827407832781688 1108 3 0.03728393504626837 1108 4 0.07492866587599423 1108 5 0.03808738414896595 1108 6 0.08941553419546722 1108 7 0.0351219003347083 1108 8 0.04623186108793931 1108 9 0.02018051410368571 1108 10-11 0.03743738927092721 1108 12-13 -0.04385562074551075 1108 14-15 0.1498238440439721 1108 16-17 0.13896448278942586 1108 18-19 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0.043630917508130196 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 9.0 4 41.0 5 109.0 6 220.0 7 599.0 8 1151.0 9 2146.0 10 3424.0 11 5387.0 12 7697.0 13 10782.0 14 14406.0 15 19223.0 16 24642.0 17 31440.0 18 30484.0 19 25899.0 20 26663.0 21 29408.0 22 31905.0 23 36133.0 24 40872.0 25 46072.0 26 53850.0 27 62565.0 28 73710.0 29 85763.0 30 100898.0 31 117030.0 32 137570.0 33 164605.0 34 199857.0 35 242597.0 36 276229.0 37 253242.0 38 88374.0 39 1985.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.52601412347895 20.123331161038287 15.296073131559174 22.05458158392359 2 15.658201266382815 21.33422411575305 40.0665594285699 22.94101518929423 3 19.057583793990926 26.00009167905743 30.427522587093993 24.514801939857648 4 12.884452346355909 17.44360801870943 37.21939746194362 32.45254217299104 5 13.039277468326487 38.66411391605117 34.0579477950038 14.238660820618534 6 30.995841112241013 37.03964628314705 17.498036266213322 14.466476338398618 7 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30.483662480994024 21.00720942077859 30-31 21.546465390443682 27.030812262599607 30.404607041849225 21.018115305107486 32-33 21.24807909607034 27.051801321679815 30.29129203569755 21.4088275465523 34-35 21.55925715748128 27.235392952120264 30.25496545047902 20.95038443991944 36-37 21.61461398803411 27.08199773873311 30.172380990315627 21.131007282917157 38-39 21.456756917350976 27.241113559367676 30.140335470742173 21.161794052539175 40-41 21.548458885056192 27.1278068320908 30.220451763658616 21.10328251919439 42-43 21.407871374124706 27.15813446016569 29.97923572976585 21.45475843594375 44-45 21.47837476151334 27.345513824994516 29.81407052396082 21.36204088953133 46-47 21.488468925255013 27.416078417672324 29.3886030377925 21.706849619280163 48-49 21.500114597495767 27.587199793501988 29.33041684560936 21.582268763392886 50-51 21.646287597435688 27.6142136497856 29.05549411547422 21.68400463730449 52-53 21.70823700097241 27.747436020106854 28.824452212844264 21.719874766076472 54-55 21.47154310534736 27.669376211340037 28.973161934049696 21.885918749262903 56-57 21.583314604616387 27.962923816029857 28.574051121609084 21.87971045774468 58-59 21.6396787709808 27.677119886782126 28.856784283009084 21.82641705922799 60-61 21.865469215552327 27.613701855144313 28.963549095569864 21.557279833733496 62-63 21.611596821532757 27.609073451431804 28.910166689289476 21.869163037745967 64-65 21.41629630157083 27.616556248802564 28.95438773882179 22.01275971080481 66-67 21.55426355381673 27.861776681396023 28.527601628313825 22.05635813647342 68-69 21.573450498392837 27.613911607729957 28.642629137650772 22.170008756226434 70-71 21.527662500361593 27.593185565610963 28.568844167432506 22.310307766594942 72-73 21.50650736629236 27.7773574627769 28.35857893575055 22.357556235180187 74-75 21.401716514722995 27.837956915791978 28.196591447689023 22.563735121796 76-77 21.26377673292553 27.726763966987022 28.58455403772594 22.42490526236151 78-79 21.352762244686794 27.771913155124956 28.43702366938956 22.43830093079869 80-81 21.17789366593769 27.954881054422042 28.380405110116 22.486820169524265 82-83 21.40187514825527 27.86907607537087 28.296140267177694 22.432908509196167 84-85 21.366448265725325 27.672110630762063 28.192747973117164 22.76869313039544 86-87 21.183862737043366 27.600372780440956 28.38612238467328 22.829642097842402 88-89 21.281044239083755 27.655379740497864 28.159742233516315 22.903833786902062 90-91 21.25680500496781 27.550910282413966 28.36291579651241 22.829368916105818 92-93 21.34312512753209 27.493659833283157 28.16562738444675 22.99758765473801 94-95 21.013443525067395 27.55699006560992 28.1591282569924 23.270438152330282 96-97 21.069428280881798 27.388912747914436 28.07022267517284 23.47143629603092 98-99 20.902293952590014 27.36646053952056 27.994210044971172 23.73703546291825 100-101 21.267328988713142 27.34640754412507 28.05833661735114 23.327926849810645 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2912.0 1 1976.5 2 1428.5 3 1777.5 4 1427.0 5 1324.0 6 2294.0 7 4716.5 8 6935.5 9 7155.0 10 5627.0 11 4049.0 12 3683.5 13 3716.0 14 3774.0 15 3838.5 16 3969.5 17 4160.5 18 4326.0 19 4575.5 20 5005.0 21 5700.0 22 6558.0 23 7752.5 24 9322.0 25 11454.5 26 14400.5 27 17818.5 28 20701.5 29 23737.5 30 27618.5 31 31705.5 32 36489.0 33 41256.0 34 46451.5 35 51785.5 36 57910.5 37 63155.5 38 65612.5 39 68012.0 40 70070.0 41 72148.5 42 74168.5 43 76312.5 44 79387.5 45 81599.5 46 81929.0 47 81868.5 48 82477.0 49 81975.0 50 80066.5 51 76882.0 52 73146.0 53 70461.0 54 67732.0 55 62830.0 56 56631.0 57 50485.0 58 45448.5 59 40941.0 60 34985.0 61 28576.5 62 22779.5 63 17294.0 64 12808.0 65 9840.0 66 7315.0 67 5516.5 68 4153.0 69 2949.0 70 2299.0 71 1784.0 72 1365.0 73 1138.0 74 918.0 75 750.5 76 531.0 77 303.0 78 194.5 79 125.5 80 78.5 81 34.5 82 13.5 83 11.0 84 7.0 85 3.5 86 3.5 87 2.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023275530208122404 2 0.012505590800157543 3 0.0011571009281284561 4 0.0 5 3.1152717295766124E-4 6 0.0 7 0.0 8 8.900776370218893E-5 9 2.2251940925547233E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.00144637616016057 26-27 0.008500241433559043 28-29 2.002674683299251E-4 30-31 0.0 32-33 2.6702329110656677E-4 34-35 0.00979085400724078 36-37 0.0010903451053518142 38-39 0.012082803922572147 40-41 0.010903451053518143 42-43 0.00473966341714156 44-45 6.230543459153225E-4 46-47 6.675582277664169E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 2.2251940925547232E-5 66-67 3.560310548087557E-4 68-69 0.0010013373416496254 70-71 0.0 72-73 1.3351164555328339E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 4.4503881851094464E-5 82-83 0.0011571009281284561 84-85 0.0059412682271211105 86-87 0.004272372657705068 88-89 0.0 90-91 0.0012238567509050976 92-93 5.117946412875864E-4 94-95 1.1125970462773616E-4 96-97 0.0 98-99 0.0 100-101 6.675582277664169E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2246995.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.364013461051684 #Duplication Level Percentage of deduplicated Percentage of total 1 72.02939018707374 36.27689176973126 2 14.864773500269028 14.972993053260671 3 5.422635787573092 8.193171053991351 4 2.5416463897355857 5.120300519435058 5 1.4536947038441779 3.660694981633386 6 0.8425019085494185 2.5459066477886783 7 0.538604350941967 1.898839374671556 8 0.3927662576015374 1.5825028067912572 9 0.2771274867387663 1.256152722528478 >10 1.4692938096049868 13.694938925907307 >50 0.11502334321602614 3.9771106912322893 >100 0.050417600065360633 4.293211512491619 >500 0.0012393936253521926 0.3691300795954383 >1k 6.196968126760042E-4 0.6562659308569332 >5k 8.852811609657203E-5 0.3887926433230829 >10k+ 1.7705623219314406E-4 1.1130972867615556 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13806 0.6144205928362101 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11159 0.4966188175763631 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8720 0.3880738497415437 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4072 0.18121980689765665 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2637 0.1173567364413361 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2463 0.10961306099924566 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 4.4503881851094464E-5 6 0.0 0.0 0.0 0.0 8.900776370218893E-5 7 0.0 0.0 0.0 0.0 8.900776370218893E-5 8 0.0 0.0 0.0 0.0 8.900776370218893E-5 9 0.0 0.0 0.0 0.0 8.900776370218893E-5 10-11 0.0 0.0 0.0 2.2251940925547232E-5 8.900776370218893E-5 12-13 0.0 0.0 0.0 4.4503881851094464E-5 1.3351164555328339E-4 14-15 0.0 0.0 0.0 6.675582277664169E-5 1.3351164555328339E-4 16-17 0.0 0.0 0.0 1.3351164555328339E-4 1.3351164555328339E-4 18-19 0.0 0.0 0.0 1.3351164555328339E-4 1.3351164555328339E-4 20-21 0.0 0.0 0.0 2.6702329110656677E-4 1.7801552740437786E-4 22-23 0.0 0.0 0.0 6.898101686919642E-4 1.7801552740437786E-4 24-25 0.0 0.0 0.0 0.0018691630377459675 1.7801552740437786E-4 26-27 0.0 0.0 0.0 0.0032042794932788015 1.7801552740437786E-4 28-29 0.0 0.0 0.0 0.0071873769189517555 1.7801552740437786E-4 30-31 0.0 0.0 0.0 0.018246591558948727 1.7801552740437786E-4 32-33 0.0 0.0 0.0 0.03834009421471788 1.7801552740437786E-4 34-35 2.2251940925547232E-5 0.0 0.0 0.0682244508777278 1.7801552740437786E-4 36-37 4.4503881851094464E-5 0.0 0.0 0.1205610159346149 1.7801552740437786E-4 38-39 4.4503881851094464E-5 0.0 0.0 0.20280418959543745 1.7801552740437786E-4 40-41 4.4503881851094464E-5 0.0 0.0 0.29127790671541326 1.7801552740437786E-4 42-43 4.4503881851094464E-5 0.0 0.0 0.37886154619836715 1.7801552740437786E-4 44-45 4.4503881851094464E-5 0.0 0.0 0.4880073164381763 1.7801552740437786E-4 46-47 4.4503881851094464E-5 0.0 0.0 0.6158892209372963 2.002674683299251E-4 48-49 4.4503881851094464E-5 0.0 0.0 0.754251789612349 2.225194092554723E-4 50-51 4.4503881851094464E-5 0.0 0.0 0.9054092243195913 2.6702329110656677E-4 52-53 4.4503881851094464E-5 0.0 0.0 1.0563663915585035 2.6702329110656677E-4 54-55 4.4503881851094464E-5 0.0 0.0 1.219161591369807 2.6702329110656677E-4 56-57 4.4503881851094464E-5 0.0 0.0 1.4094824421060128 2.89275232032114E-4 58-59 4.4503881851094464E-5 0.0 0.0 1.6198522916161362 3.1152717295766124E-4 60-61 4.4503881851094464E-5 0.0 0.0 1.8450641857235999 3.1152717295766124E-4 62-63 4.4503881851094464E-5 0.0 0.0 2.070676614767723 3.560310548087557E-4 64-65 4.4503881851094464E-5 0.0 0.0 2.300450156764924 3.560310548087557E-4 66-67 4.4503881851094464E-5 0.0 0.0 2.561754699053625 3.560310548087557E-4 68-69 4.4503881851094464E-5 0.0 0.0 2.8588403623506062 3.560310548087557E-4 70-71 4.4503881851094464E-5 0.0 0.0 3.1929087514658465 3.782829957343029E-4 72-73 4.4503881851094464E-5 0.0 0.0 3.517453309864953 4.450388185109446E-4 74-75 4.4503881851094464E-5 0.0 0.0 3.841619585268325 5.117946412875863E-4 76-77 4.4503881851094464E-5 0.0 0.0 4.1964935391489515 5.340465822131335E-4 78-79 4.4503881851094464E-5 0.0 0.0 4.592110796864256 5.340465822131335E-4 80-81 4.4503881851094464E-5 4.4503881851094464E-5 0.0 5.023709443056171 5.340465822131335E-4 82-83 4.4503881851094464E-5 4.4503881851094464E-5 0.0 5.45817858962748 5.340465822131335E-4 84-85 4.4503881851094464E-5 4.4503881851094464E-5 0.0 5.891223611979555 5.78550464064228E-4 86-87 4.4503881851094464E-5 4.4503881851094464E-5 0.0 6.344072861755366 6.008024049897752E-4 88-89 4.4503881851094464E-5 4.4503881851094464E-5 0.0 6.825538107561432 6.230543459153225E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6330 0.0 52.84459 1 GTATCAA 11150 0.0 40.270638 1 ATCAACG 14740 0.0 30.226786 3 TATCAAC 14805 0.0 30.13018 2 TCAACGC 14860 0.0 30.013988 4 CAACGCA 15110 0.0 29.485962 5 AACGCAG 15625 0.0 28.483706 6 ACGCAGA 18055 0.0 24.623812 7 CGCAGAG 18120 0.0 24.483055 8 GTACATG 13485 0.0 24.312506 1 TACATGG 13445 0.0 23.991287 2 ACATGGG 13500 0.0 23.08111 3 GCAGAGT 19350 0.0 22.460377 9 GTGGTAT 3035 0.0 21.604885 1 GAGTACT 10935 0.0 21.479513 12-13 AGTACTT 11365 0.0 21.398209 12-13 TGGTATC 2980 0.0 21.361568 2 CATGGGA 7985 0.0 20.641035 4 GTACTTT 11265 0.0 20.51297 14-15 AGAGTAC 18585 0.0 19.947445 10-11 >>END_MODULE