FastQCFastQC Report
Thu 26 May 2016
SRR938483_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938483_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2423938
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51210.2112677799514674No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40880.16865117837172403No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28340.11691718187511396No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31600.051.4757841
GTATCAA59050.044.3003041
ATCAACG74300.034.375533
TATCAAC76600.033.5917362
TCAACGC76400.033.555164
CAACGCA78350.032.7200285
AACGCAG81150.031.5662676
TGGTATC14700.028.7962952
ACGCAGA95050.026.8022657
CGCAGAG95550.026.5625298
GTGGTAT18150.025.6810861
GTACATG73850.025.5040191
TACATGG74350.024.8207382
GCAGAGT104950.023.277199
ACATGGG78000.023.0494793
TAAGGTG11950.021.094635
GAGTACT63600.020.17491312-13
AGAGTAC95750.020.05339810-11
CAGAGTA100750.019.76579910-11
CATGGGG52500.019.4779424
Produced by FastQC (version 0.11.5)