##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938483_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2423938 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.676520604074856 31.0 31.0 34.0 27.0 34.0 2 30.731578530473964 31.0 31.0 34.0 27.0 34.0 3 30.766422656025032 31.0 31.0 34.0 27.0 34.0 4 34.400026733357045 37.0 35.0 37.0 30.0 37.0 5 34.35771459501027 37.0 35.0 37.0 30.0 37.0 6 34.35326563633228 37.0 35.0 37.0 30.0 37.0 7 34.24219720141357 37.0 35.0 37.0 30.0 37.0 8 34.19775299533239 37.0 35.0 37.0 30.0 37.0 9 35.62414261420878 38.0 35.0 39.0 30.0 39.0 10-11 35.43302159543685 38.0 35.0 39.0 29.0 39.0 12-13 35.269341253777945 38.0 35.0 39.0 29.0 39.0 14-15 36.152514008196576 39.0 35.5 40.5 27.0 41.0 16-17 35.94302865832377 38.0 35.0 40.0 27.0 41.0 18-19 36.07650835128621 39.0 35.0 40.5 27.0 41.0 20-21 36.03971925024485 39.0 35.0 41.0 27.0 41.0 22-23 35.92643438074737 39.0 35.0 40.5 27.0 41.0 24-25 35.760607738316736 39.0 34.5 40.5 27.0 41.0 26-27 35.51766175537493 38.5 34.0 40.0 26.5 41.0 28-29 35.28128813525758 38.0 34.0 40.0 25.5 41.0 30-31 35.0040229989381 38.0 34.0 40.0 25.0 41.0 32-33 34.70617812831846 38.0 33.5 40.0 24.0 41.0 34-35 34.426694288385264 38.0 33.0 40.0 24.0 41.0 36-37 34.116986284302655 38.0 33.0 40.0 23.5 41.0 38-39 33.94275719923529 38.0 33.0 40.0 22.5 41.0 40-41 33.80686552213794 38.0 33.0 40.0 21.5 41.0 42-43 33.48923322296197 38.0 32.5 40.0 18.5 41.0 44-45 33.12277026062548 37.5 31.5 40.0 17.0 41.0 46-47 32.71183008806331 37.0 31.0 40.0 14.5 41.0 48-49 32.32650855756212 37.0 30.5 40.0 10.5 41.0 50-51 31.64575269664488 36.0 30.0 39.0 9.0 40.5 52-53 31.680632095375376 36.0 30.0 39.0 8.0 40.0 54-55 31.738267439183677 36.5 30.0 39.0 7.5 41.0 56-57 31.37803565932792 36.0 29.5 39.0 4.0 41.0 58-59 30.957292018195183 36.0 29.0 39.0 2.0 40.5 60-61 30.437223435582922 35.0 28.0 39.0 2.0 40.0 62-63 29.813689747840087 35.0 27.5 38.5 2.0 40.0 64-65 29.244399196679126 34.0 26.5 38.0 2.0 40.0 66-67 28.65690995396747 34.0 26.0 37.5 2.0 40.0 68-69 28.011133948145538 34.0 25.5 37.0 2.0 39.0 70-71 27.32588622316247 33.0 23.5 36.0 2.0 39.0 72-73 26.63770298580244 32.5 21.5 36.0 2.0 39.0 74-75 25.908666187006432 32.0 19.5 35.0 2.0 38.0 76-77 25.10640247399067 31.0 16.0 35.0 2.0 37.0 78-79 24.281134872261585 31.0 9.0 35.0 2.0 36.5 80-81 23.542349268009332 30.0 4.5 34.0 2.0 36.0 82-83 22.769491216359494 29.5 2.0 34.0 2.0 35.5 84-85 22.024801789484712 29.0 2.0 34.0 2.0 35.0 86-87 21.228757294947314 29.0 2.0 34.0 2.0 35.0 88-89 20.373180337120836 27.0 2.0 34.0 2.0 35.0 90-91 19.4361893744807 26.0 2.0 33.0 2.0 35.0 92-93 18.47175237155406 24.5 2.0 32.5 2.0 34.5 94-95 17.457396393802153 21.5 2.0 32.0 2.0 34.0 96-97 16.220294826022776 10.0 2.0 32.0 2.0 34.0 98-99 14.754131706339024 2.0 2.0 31.0 2.0 34.0 100-101 11.619469433624127 2.0 2.0 27.0 2.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.513014176076215 1101 2 0.5812451857872176 1101 3 0.5515644699862854 1101 4 0.3985929071549137 1101 5 0.5497250740235202 1101 6 0.5913422940019757 1101 7 0.49363552287703527 1101 8 0.6297731115118523 1101 9 0.881563947959549 1101 10-11 0.9238764462462967 1101 12-13 0.9268318795153903 1101 14-15 1.1155202889626281 1101 16-17 1.0792490515644815 1101 18-19 0.9355205681543666 1101 20-21 1.000799574333648 1101 22-23 1.1237731597813365 1101 24-25 1.0528351702047871 1101 26-27 1.0643567205949935 1101 28-29 1.147908972123716 1101 30-31 1.2344219511509635 1101 32-33 1.2685189705472695 1101 34-35 1.085821906165485 1101 36-37 1.3441445368184688 1101 38-39 1.3704778185487285 1101 40-41 1.367396412313056 1101 42-43 1.4043921837794713 1101 44-45 1.5428275734856456 1101 46-47 1.5893237623181236 1101 48-49 1.4760502787310728 1101 50-51 1.5959182583762441 1101 52-53 1.4980475669185545 1101 54-55 1.5458607733561287 1101 56-57 1.5966323567093355 1101 58-59 1.6447426411501773 1101 60-61 1.7542964857938443 1101 62-63 1.6951383175502563 1101 64-65 1.7244519354071208 1101 66-67 1.604800477035493 1101 68-69 1.6330422525771056 1101 70-71 1.680317277885262 1101 72-73 1.8418883043370897 1101 74-75 1.7140564870697865 1101 76-77 1.7391445411399857 1101 78-79 2.073491147533286 1101 80-81 1.9820996125412975 1101 82-83 1.8563484025492727 1101 84-85 1.9503305525430683 1101 86-87 2.0318742949727238 1101 88-89 1.9609949585835977 1101 90-91 1.5464193429922553 1101 92-93 1.5499956168239102 1101 94-95 1.4798506343206732 1101 96-97 1.4752697030763642 1101 98-99 1.5097648852214078 1101 100-101 1.438002170448426 1102 1 0.6057610543953622 1102 2 0.5733864657890955 1102 3 0.5526176926886066 1102 4 0.5370332432919156 1102 5 0.40748828591510033 1102 6 0.5599578110663046 1102 7 0.5391245505283493 1102 8 0.4696574938428739 1102 9 0.7281060467430862 1102 10-11 0.8667758230279574 1102 12-13 0.889082210411857 1102 14-15 1.1121325078616806 1102 16-17 1.1076444774821326 1102 18-19 1.0157642398186724 1102 20-21 1.0631637533182285 1102 22-23 0.9837748725234121 1102 24-25 1.151250012251701 1102 26-27 1.1037323578998794 1102 28-29 0.9622091748866097 1102 30-31 1.21991830342715 1102 32-33 1.131329656087452 1102 34-35 1.0328076127265362 1102 36-37 1.1903380243819583 1102 38-39 1.2047165937410966 1102 40-41 1.1967714925978399 1102 42-43 1.2381460293969937 1102 44-45 1.3669149415202 1102 46-47 1.3228503664697016 1102 48-49 1.3834160359392698 1102 50-51 1.37037712471529 1102 52-53 1.284213896910142 1102 54-55 1.0796982288276773 1102 56-57 1.347984267231439 1102 58-59 1.2639789514338666 1102 60-61 1.320053277176168 1102 62-63 1.2964937141845638 1102 64-65 1.4598615138035314 1102 66-67 1.3019933609710712 1102 68-69 1.3625387026160034 1102 70-71 1.5928681533351892 1102 72-73 1.8398612437700592 1102 74-75 1.7912969867509183 1102 76-77 1.9209707751194998 1102 78-79 1.9990060974602066 1102 80-81 2.1137938479137404 1102 82-83 2.0727431335180384 1102 84-85 2.3036989895105577 1102 86-87 2.244281121992973 1102 88-89 2.2017045643005204 1102 90-91 2.169535374068669 1102 92-93 2.1973504534067168 1102 94-95 2.424812880104465 1102 96-97 2.385914963498415 1102 98-99 2.5078191988494893 1102 100-101 2.2955086604904604 1103 1 0.6758794616797061 1103 2 0.5780401474295402 1103 3 0.6395895870215789 1103 4 0.5326670435232259 1103 5 0.5394556826070414 1103 6 0.6841114284547345 1103 7 0.6658034556740162 1103 8 0.7325047759826475 1103 9 0.7885154238656966 1103 10-11 0.9652181706129923 1103 12-13 0.8084273905928683 1103 14-15 1.0215995832935434 1103 16-17 1.0375044080899514 1103 18-19 1.1236560394258177 1103 20-21 0.8380999602464314 1103 22-23 1.0272327323805897 1103 24-25 1.1186127424115924 1103 26-27 1.0957880552178167 1103 28-29 1.0630308663667947 1103 30-31 1.1363293694598653 1103 32-33 1.1286813716162456 1103 34-35 0.9893583809970536 1103 36-37 1.2495185358279954 1103 38-39 1.156833605838834 1103 40-41 1.2472394308424768 1103 42-43 1.3830618592576585 1103 44-45 1.1622164670466049 1103 46-47 1.0503509630955605 1103 48-49 1.0263615318730288 1103 50-51 1.286362291678742 1103 52-53 1.2455966002677705 1103 54-55 1.2451370700884858 1103 56-57 1.186334091915235 1103 58-59 1.229241375019857 1103 60-61 1.246050193079462 1103 62-63 1.376630295610223 1103 64-65 1.5555582810093043 1103 66-67 1.3219026738416666 1103 68-69 1.4444798477704843 1103 70-71 1.4067561345368844 1103 72-73 1.5148358183299315 1103 74-75 1.8074884944703378 1103 76-77 1.681921337783571 1103 78-79 1.6067354312586026 1103 80-81 1.7512590141428817 1103 82-83 1.7029443341128285 1103 84-85 1.8823273007840307 1103 86-87 2.0424161613702765 1103 88-89 1.958539693630648 1103 90-91 1.9280327948092228 1103 92-93 1.6906131707162118 1103 94-95 1.8892262592097104 1103 96-97 2.0847863309648673 1103 98-99 1.7870483582495638 1103 100-101 1.5433275799551485 1104 1 0.5024903787256818 1104 2 0.4711631847253308 1104 3 0.4920104613732228 1104 4 0.41352975126113023 1104 5 0.36558320104322917 1104 6 0.5964878442481094 1104 7 0.5041609589378382 1104 8 0.6290553564138222 1104 9 0.631770425453503 1104 10-11 0.7677516027431253 1104 12-13 0.80106332439901 1104 14-15 0.9447568466222904 1104 16-17 1.0602260834013393 1104 18-19 1.082854550416961 1104 20-21 1.0252466734887307 1104 22-23 1.155310958272409 1104 24-25 1.0957906824983965 1104 26-27 1.0945089895532263 1104 28-29 0.9590156587170995 1104 30-31 1.0792311725013946 1104 32-33 1.1050158340558127 1104 34-35 1.0778307355364163 1104 36-37 1.057810233368663 1104 38-39 1.2425437078112438 1104 40-41 1.2132822663105216 1104 42-43 1.2533293162986965 1104 44-45 1.1651646692977593 1104 46-47 1.205505862358116 1104 48-49 0.92329036289523 1104 50-51 1.01226046040955 1104 52-53 1.1466392593129733 1104 54-55 1.301485646356081 1104 56-57 1.3717259270657252 1104 58-59 1.4283714339450917 1104 60-61 1.3999884803664315 1104 62-63 1.6039948910726274 1104 64-65 1.455332283338084 1104 66-67 1.6966084005273885 1104 68-69 1.937979195005937 1104 70-71 2.01813576122613 1104 72-73 1.815204365664183 1104 74-75 1.6882795745310268 1104 76-77 1.8161819496180165 1104 78-79 1.7944482854274781 1104 80-81 1.8045076645080762 1104 82-83 1.8949400293952792 1104 84-85 1.8534631110941824 1104 86-87 1.9625003150698674 1104 88-89 2.162692334680713 1104 90-91 2.0674038473479435 1104 92-93 2.1195588677628905 1104 94-95 2.2278538658573233 1104 96-97 2.393930680140972 1104 98-99 2.48984554254087 1104 100-101 1.9304485354613075 1105 1 0.582986341655026 1105 2 0.5540986216499846 1105 3 0.3743095979897788 1105 4 0.42385118076283845 1105 5 0.47785878112376423 1105 6 0.6571130308403212 1105 7 0.7515785561444446 1105 8 0.600938122740601 1105 9 0.8714525023825885 1105 10-11 0.8750762695153256 1105 12-13 0.8719732899424883 1105 14-15 1.0166313310934783 1105 16-17 0.888179466572744 1105 18-19 0.8108778284421945 1105 20-21 1.0280816227857414 1105 22-23 1.0620922451525416 1105 24-25 1.0078549082884862 1105 26-27 0.974655845553805 1105 28-29 0.9311556467885538 1105 30-31 1.1370219942017243 1105 32-33 1.1082241347459458 1105 34-35 0.9740840474437888 1105 36-37 1.1528108812234876 1105 38-39 1.10728998581844 1105 40-41 1.2805152382477232 1105 42-43 1.3346411087928018 1105 44-45 1.3454837912340594 1105 46-47 1.1341340497871926 1105 48-49 1.0966990611144283 1105 50-51 1.1936786814269027 1105 52-53 1.1160471533907064 1105 54-55 1.1368419397982557 1105 56-57 1.135988250834174 1105 58-59 1.3265230606952194 1105 60-61 1.2087565109344922 1105 62-63 1.2874441703842194 1105 64-65 1.4821747624447674 1105 66-67 1.6056813903907958 1105 68-69 1.5811713928300826 1105 70-71 1.523031573479141 1105 72-73 1.6395225819394952 1105 74-75 1.5030652247468588 1105 76-77 1.526910926561733 1105 78-79 1.6014155968098862 1105 80-81 1.8666270915372465 1105 82-83 2.0784068408608434 1105 84-85 2.085667974814246 1105 86-87 2.008721334419352 1105 88-89 2.046157109076958 1105 90-91 1.9471088095820157 1105 92-93 1.8839898341552583 1105 94-95 1.9114443207516025 1105 96-97 2.15212988414779 1105 98-99 2.1308508963597745 1105 100-101 1.751909511558134 1106 1 0.4567296623615924 1106 2 0.29499668793275546 1106 3 0.3381075620766012 1106 4 0.3041693524438571 1106 5 0.4195463131365784 1106 6 0.26571170466206695 1106 7 0.5102227729240241 1106 8 0.4171295224329512 1106 9 0.36246734652764445 1106 10-11 0.31518364469697957 1106 12-13 0.4281706040925002 1106 14-15 0.6359839430535885 1106 16-17 0.40281578869502255 1106 18-19 0.36340270551705345 1106 20-21 0.5471738025493167 1106 22-23 0.510860543005947 1106 24-25 0.38527581262867017 1106 26-27 0.4947458041280939 1106 28-29 0.6132975790680177 1106 30-31 0.4330420706812177 1106 32-33 0.6106165492089204 1106 34-35 0.3556900024203884 1106 36-37 0.4197105862358299 1106 38-39 0.511376353238596 1106 40-41 0.7787840340102221 1106 42-43 0.5711223659254827 1106 44-45 0.8518276189174756 1106 46-47 0.813393991018394 1106 48-49 0.9510760457049798 1106 50-51 0.620682346920205 1106 52-53 0.42406753376222284 1106 54-55 0.6001260649527573 1106 56-57 -0.25923623305995136 1106 58-59 0.07940623626883792 1106 60-61 0.7116878435908767 1106 62-63 0.7387247239905257 1106 64-65 0.7353679568972638 1106 66-67 0.5140074599967761 1106 68-69 0.6282026046648745 1106 70-71 0.4190801602586909 1106 72-73 0.8719792303535812 1106 74-75 1.1775717882427266 1106 76-77 1.0576768079833911 1106 78-79 1.2855438555821124 1106 80-81 1.1497515263348461 1106 82-83 0.8354396946928659 1106 84-85 0.9862313187375094 1106 86-87 1.0394288064870203 1106 88-89 1.031606889694693 1106 90-91 1.2141529449969575 1106 92-93 1.137799308560929 1106 94-95 1.3392918584501423 1106 96-97 1.1612206669883456 1106 98-99 1.3997970527383607 1106 100-101 0.932388043465572 1107 1 0.1991966422597038 1107 2 0.043289486445530656 1107 3 0.08366508515747739 1107 4 -0.15459152007719723 1107 5 0.11029782211011252 1107 6 0.15662717113387004 1107 7 0.045495024270394424 1107 8 -0.020125565060084227 1107 9 0.19041438714251058 1107 10-11 0.32836695665434945 1107 12-13 0.33255968748102305 1107 14-15 0.42584155765487 1107 16-17 0.5065787802594031 1107 18-19 0.3807149316416414 1107 20-21 0.3960150873972381 1107 22-23 0.26110914296634746 1107 24-25 0.49793958128162075 1107 26-27 0.5032398680238046 1107 28-29 0.48092630995587626 1107 30-31 0.381373115341745 1107 32-33 0.49345396809489017 1107 34-35 0.46720678420981443 1107 36-37 0.4595374687454168 1107 38-39 0.3910794168265923 1107 40-41 0.4470988101430393 1107 42-43 0.6674955815359525 1107 44-45 0.42677321158566883 1107 46-47 0.47083095842488376 1107 48-49 0.2964318422227521 1107 50-51 0.3908920964278195 1107 52-53 0.5157297145241806 1107 54-55 0.6603472087908706 1107 56-57 0.6343664029822342 1107 58-59 0.5133718369997275 1107 60-61 0.5126384313172814 1107 62-63 0.740128381467251 1107 64-65 0.76262593074199 1107 66-67 0.8334435797859392 1107 68-69 0.7807800054057878 1107 70-71 0.939280884947653 1107 72-73 0.988048575946884 1107 74-75 0.8672189297606288 1107 76-77 1.3047109822283858 1107 78-79 1.3498023136660038 1107 80-81 1.3508093782053692 1107 82-83 1.109225975855324 1107 84-85 1.1450471353086655 1107 86-87 1.1742514866911797 1107 88-89 0.9913835512751241 1107 90-91 0.9667320950419231 1107 92-93 1.1697264929844913 1107 94-95 1.2473952709447822 1107 96-97 1.3963522583859032 1107 98-99 1.3871609548950161 1107 100-101 0.9755514054053283 1108 1 0.35473700284307697 1108 2 0.2516998409037541 1108 3 0.18490933098839335 1108 4 0.10277678417487124 1108 5 0.23297052862160683 1108 6 0.19238371447746516 1108 7 0.25295113377944034 1108 8 0.398953038068143 1108 9 0.21168795725116496 1108 10-11 0.511905550013033 1108 12-13 0.3279033386041874 1108 14-15 0.3577978475041519 1108 16-17 0.5197200471953707 1108 18-19 0.4943581755858304 1108 20-21 0.4035367552413831 1108 22-23 0.22959619409886756 1108 24-25 0.5598402906303264 1108 26-27 0.5579462313638928 1108 28-29 0.33310448015790683 1108 30-31 0.46762993643906015 1108 32-33 0.48774768353130327 1108 34-35 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2315 46-47 -2.0405257522512876 2315 48-49 -1.9867201123419989 2315 50-51 -1.8399577385365404 2315 52-53 -1.8239331743765703 2315 54-55 -2.0893964814663413 2315 56-57 -1.969113944091312 2315 58-59 -1.8072171419132879 2315 60-61 -2.205062264430296 2315 62-63 -2.3933651406229366 2315 64-65 -2.2580617303214296 2315 66-67 -2.3024845224317794 2315 68-69 -2.396535669036769 2315 70-71 -2.5847184342658345 2315 72-73 -2.681282934030637 2315 74-75 -2.7019616503112296 2315 76-77 -2.8243110366341533 2315 78-79 -2.9901387031994098 2315 80-81 -3.180715995299394 2315 82-83 -3.219248949281358 2315 84-85 -3.379134993059605 2315 86-87 -3.422725000659181 2315 88-89 -3.3175129627963997 2315 90-91 -3.4076187222498255 2315 92-93 -3.5034098684377604 2315 94-95 -3.5163347693689104 2315 96-97 -3.388352124278743 2315 98-99 -3.3332753544534484 2315 100-101 -2.8126119265285467 2316 1 -0.8589773888725922 2316 2 -0.9413341831809419 2316 3 -0.8580218713284076 2316 4 -0.9694660510944857 2316 5 -0.830837649873736 2316 6 -0.909733864637964 2316 7 -0.8594570267660018 2316 8 -0.9480061180819632 2316 9 -1.1052553771637648 2316 10-11 -1.0037069696590777 2316 12-13 -1.2071489637719566 2316 14-15 -1.3882762099829904 2316 16-17 -1.2519210758686725 2316 18-19 -1.1494342622190814 2316 20-21 -1.2572434865301076 2316 22-23 -1.317181481856025 2316 24-25 -1.5100475194296052 2316 26-27 -1.459121866933181 2316 28-29 -1.528114648923264 2316 30-31 -1.5632956125565372 2316 32-33 -1.3931452824752242 2316 34-35 -1.4078427315882678 2316 36-37 -1.53569590859869 2316 38-39 -1.9547571034343107 2316 40-41 -1.9047700964686172 2316 42-43 -1.8730725461645932 2316 44-45 -1.7093229290241752 2316 46-47 -1.951291698796794 2316 48-49 -2.1527276188215296 2316 50-51 -2.065638923213278 2316 52-53 -2.136924140816358 2316 54-55 -2.4914799352026904 2316 56-57 -2.410679351824079 2316 58-59 -2.263184092953349 2316 60-61 -2.462583053399751 2316 62-63 -2.3363198138899115 2316 64-65 -2.63633886879008 2316 66-67 -2.8514780829081765 2316 68-69 -2.9318684425887085 2316 70-71 -3.157377855264418 2316 72-73 -3.1083280162529263 2316 74-75 -2.994751862324261 2316 76-77 -3.1166837743541578 2316 78-79 -3.234178615131089 2316 80-81 -3.5834399004470114 2316 82-83 -3.885527779319723 2316 84-85 -3.726222981358653 2316 86-87 -3.809903163405526 2316 88-89 -4.089036436938276 2316 90-91 -4.010905613362526 2316 92-93 -3.8083597659655357 2316 94-95 -3.9430053056031795 2316 96-97 -3.9745328499421433 2316 98-99 -3.7437203806605535 2316 100-101 -3.1924607410960526 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15679.0 3 5839.0 4 6118.0 5 6596.0 6 7596.0 7 9108.0 8 11161.0 9 13603.0 10 16235.0 11 19139.0 12 22170.0 13 25036.0 14 27186.0 15 30129.0 16 33835.0 17 36616.0 18 36868.0 19 36953.0 20 39220.0 21 41886.0 22 46021.0 23 50308.0 24 55567.0 25 60430.0 26 66709.0 27 74307.0 28 82603.0 29 92293.0 30 104017.0 31 118576.0 32 135657.0 33 158016.0 34 184500.0 35 216188.0 36 237633.0 37 210932.0 38 85476.0 39 3732.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.29184292423424 18.223282460857515 14.47503540966914 24.009839205239107 2 15.679344223567956 19.393362196461176 40.3414593652287 24.585834214742167 3 19.547288048645697 23.261801050597704 28.937655559584947 28.253255341171652 4 13.192506859652523 16.055201047813732 36.0430905548481 34.709201537685644 5 14.35769510956002 36.61559554214742 33.91040577433309 15.116303573959469 6 33.42838135219813 35.03315806230883 17.09196009387248 14.446500491620565 7 29.576768485428722 31.517625017538357 20.196429603096654 18.70917689393627 8 26.421299100886568 35.1511755161195 19.38268690813593 19.044838474858004 9 26.33950825138073 16.872043351523015 19.639491085075548 37.148957312020706 10-11 25.34108791912996 26.059990031587276 27.107436993130463 21.491485056152296 12-13 26.156559391891754 23.25436154206543 27.372259911376023 23.216819154666794 14-15 22.89903581986262 24.9753791446654 25.38940980307236 26.736175232399624 16-17 22.83612597465947 28.018627788411305 26.151268140613432 22.993978096315793 18-19 22.86407102852569 26.64436398033616 28.195874044299558 22.295690946838594 20-21 23.20932920536636 25.985304437564498 28.376449948400413 22.42891640866873 22-23 23.001155317462626 25.832064836440765 28.2590156133964 22.90776423270021 24-25 22.886044662824652 26.063096909793508 28.2623088588451 22.78854956853674 26-27 22.498758538018045 26.424688561253916 28.100569359438644 22.975983541289395 28-29 22.64358381605487 26.740692109894372 28.0813601550873 22.53436391896346 30-31 22.710037863032962 26.286023085241673 28.049648193533084 22.95429085819228 32-33 22.503675125319557 26.292309860893177 28.016889375580572 23.18712563820669 34-35 23.177160680992053 26.404366017437404 27.710096838451697 22.70837646311885 36-37 23.240082083719532 25.9860644249465 27.796479761715332 22.977373729618638 38-39 22.66780925866812 26.30423967524856 27.85713858288741 23.170812483195913 40-41 23.089164614135065 26.17041762689928 27.874177061391052 22.8662406975746 42-43 22.886487447941782 26.171930213358475 27.69644696927959 23.245135369420154 44-45 22.83260646276333 26.476386866365768 27.786796185162103 22.9042104857088 46-47 23.189919295692505 26.64748171161047 27.208444626084212 22.954154366612816 48-49 23.024923835583884 26.547858154296065 27.308264540554454 23.11895346956559 50-51 23.151923110643974 26.786838948444398 27.207820137659013 22.853417803252615 52-53 23.257248433796978 26.591329935767522 27.152869006674102 22.998552623761395 54-55 22.86233396928927 26.73104757786609 27.29581605909769 23.110802393746948 56-57 22.982094813377696 26.848949989715592 27.210234977594016 22.958720219312696 58-59 22.927993503471086 26.50382842957637 27.280758654611116 23.28741941234142 60-61 22.759728878269016 26.7093818261948 27.387990828358966 23.142898467177215 62-63 22.73387715509194 26.738622890809914 27.536428018985422 22.991071935112732 64-65 22.567909404890937 26.674800213062056 27.40174451779856 23.35554586424844 66-67 22.543105520151325 26.751467657888877 27.23740337244131 23.468023449518487 68-69 22.85298798222027 26.367859504988118 27.552535237011476 23.226617275780132 70-71 22.74782902956564 26.4908224462403 27.216773087291735 23.544575436902317 72-73 22.78663189214396 26.48324756626486 27.212976069130875 23.51714447246031 74-75 22.757941056369983 26.880668995942784 26.990708825285104 23.370681122402132 76-77 22.894614893563464 26.543543306859167 27.081038834779186 23.48080296479818 78-79 22.8957568781317 26.586276536272514 27.152514331804745 23.365452253791045 80-81 23.012455372494625 26.699001387689275 26.897944086869717 23.39059915294638 82-83 22.90407658277919 26.739858052444887 26.912501431130885 23.443563933645038 84-85 22.925911340658317 26.66121518413916 26.936134108120473 23.476739367082054 86-87 23.060945557172758 26.571058106801974 26.796961954284903 23.571034381740365 88-89 22.933659650907607 26.580960153693926 26.74928471861816 23.73609547678031 90-91 22.860082461624557 26.599166799590517 26.768559591740466 23.77219114704446 92-93 22.897799655085752 26.23201198740019 26.94008074410359 23.93010761341047 94-95 22.735917978181998 26.152626951794844 27.064847261605518 24.04660780841764 96-97 22.985340747397977 25.914694231743518 26.974077257470213 24.12588776338829 98-99 22.85021014775337 26.049841228575144 26.655916840500225 24.444031783171262 100-101 23.5151097284811 25.360521692818054 26.89925953144256 24.225109047258282 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 302.0 1 227.5 2 210.5 3 267.5 4 234.5 5 239.5 6 374.0 7 774.0 8 1224.5 9 1437.5 10 1403.5 11 1246.0 12 1273.5 13 1404.0 14 1559.0 15 1702.0 16 1830.5 17 2066.0 18 2305.5 19 2539.5 20 2933.5 21 3473.0 22 4246.5 23 5201.5 24 6502.5 25 8328.5 26 10595.0 27 13544.0 28 16579.0 29 19619.0 30 23228.5 31 28183.5 32 34549.0 33 38394.5 34 40616.0 35 44367.5 36 49546.0 37 55213.5 38 59734.0 39 63102.5 40 66635.5 41 71636.5 42 76304.5 43 79835.0 44 83043.0 45 87041.5 46 90063.5 47 92128.0 48 93178.0 49 92987.5 50 92819.5 51 91142.0 52 89930.0 53 89856.5 54 89302.0 55 84838.0 56 77273.5 57 70211.5 58 64419.0 59 57313.0 60 47816.5 61 40146.0 62 33943.5 63 26825.0 64 20166.0 65 15339.0 66 11692.5 67 9097.5 68 7088.0 69 5354.0 70 4094.0 71 3124.5 72 2362.5 73 1833.0 74 1395.0 75 1037.5 76 782.0 77 544.5 78 327.0 79 191.0 80 128.5 81 93.0 82 70.0 83 36.0 84 17.5 85 11.0 86 7.0 87 5.0 88 2.5 89 1.5 90 3.0 91 3.0 92 4.5 93 3.0 94 1.0 95 2.0 96 2.0 97 1.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.006807104802185534 2 0.10829484912567897 3 0.1171647129588298 4 0.11951625825413026 5 0.15879119020370983 6 0.09715595035846626 7 0.027971012459889647 8 0.02731092956998075 9 0.024175535842913473 10-11 0.05303353468611821 12-13 0.055405707571728316 14-15 0.02836293667577306 16-17 0.032344061605536115 18-19 0.037604097134497666 20-21 0.05932494973056241 22-23 0.032777241002038826 24-25 0.051651486135371454 26-27 0.05738595624145502 28-29 0.05373487275664642 30-31 0.06825669633464222 32-33 0.050805754932675674 34-35 0.061944653699888365 36-37 0.05319855540859543 38-39 0.03269473064080022 40-41 0.037707235086045926 42-43 0.03339606871132843 44-45 0.00790036708859715 46-47 0.013923623459015865 48-49 0.009653712264917666 50-51 0.008065387811074376 52-53 0.009983753709872117 54-55 0.011964002379598819 56-57 0.015120023696975748 58-59 0.020215038503460073 60-61 0.0246912256006548 62-63 0.011902119608669859 64-65 0.009529946723059748 66-67 0.010870740093187201 68-69 0.01151019539278645 70-71 0.03178711666717548 72-73 0.02920866787846884 74-75 0.029889378358687393 76-77 0.02347419777238527 78-79 0.005486939022367734 80-81 0.005136269987103631 82-83 0.006415180586302125 84-85 0.005466311432058081 86-87 0.014047389000873786 88-89 0.03880049737245755 90-91 0.035953889909725416 92-93 0.029992516310235657 94-95 0.04818605096334972 96-97 0.04395739494987083 98-99 0.026527081138213934 100-101 0.07520819426899533 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2423938.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.950625678296525 #Duplication Level Percentage of deduplicated Percentage of total 1 81.66585972811852 50.59251106712941 2 10.85289460867942 13.446872228566026 3 3.200647232733536 5.948462959300528 4 1.362545558676272 3.3764219950071652 5 0.7429739805212 2.3013851477991416 6 0.44226883631865965 1.6439298676771847 7 0.3036246548875252 1.3166816139147328 8 0.2223950131002149 1.1022008167433013 9 0.17251006838605176 0.9618396005089465 >10 0.9385435633298272 10.660561117448285 >50 0.06026250413225192 2.5612149497294556 >100 0.03179173239330127 3.511162016999251 >500 0.002343421005627182 1.0559716366349334 >1k 0.001272142831624452 1.308371173475656 >5k 6.695488587497117E-5 0.21241380906599988 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5121 0.2112677799514674 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4088 0.16865117837172403 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2834 0.11691718187511396 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.125518061930627E-5 0.0 5 0.0 0.0 0.0 4.125518061930627E-5 0.0 6 0.0 0.0 0.0 4.125518061930627E-5 0.0 7 0.0 0.0 0.0 4.125518061930627E-5 0.0 8 0.0 0.0 0.0 4.125518061930627E-5 0.0 9 0.0 0.0 0.0 4.125518061930627E-5 0.0 10-11 0.0 0.0 0.0 4.125518061930627E-5 0.0 12-13 0.0 0.0 0.0 1.0313795154826566E-4 4.125518061930627E-5 14-15 0.0 0.0 0.0 1.237655418579188E-4 4.125518061930627E-5 16-17 0.0 0.0 0.0 2.269034934061845E-4 4.125518061930627E-5 18-19 0.0 0.0 0.0 2.6815867402549075E-4 4.125518061930627E-5 20-21 0.0 0.0 0.0 3.300414449544502E-4 4.125518061930627E-5 22-23 0.0 0.0 0.0 4.744345771220221E-4 4.125518061930627E-5 24-25 0.0 0.0 0.0 8.044760220764723E-4 4.125518061930627E-5 26-27 0.0 0.0 0.0 9.07613973624738E-4 4.125518061930627E-5 28-29 0.0 0.0 0.0 0.002330917704990804 4.125518061930627E-5 30-31 0.0 0.0 0.0 0.00862233274943501 4.125518061930627E-5 32-33 0.0 0.0 0.0 0.022958508014643938 4.125518061930627E-5 34-35 2.0627590309653136E-5 0.0 0.0 0.04323542928903297 6.188277092895941E-5 36-37 4.125518061930627E-5 0.0 0.0 0.07898304329566186 8.251036123861254E-5 38-39 4.125518061930627E-5 0.0 0.0 0.13313046785850133 8.251036123861254E-5 40-41 4.125518061930627E-5 0.0 0.0 0.1927854590340182 8.251036123861254E-5 42-43 4.125518061930627E-5 0.0 0.0 0.2547919955048355 8.251036123861254E-5 44-45 4.125518061930627E-5 0.0 0.0 0.33297056277842085 8.251036123861254E-5 46-47 4.125518061930627E-5 0.0 0.0 0.42581534676216964 8.251036123861254E-5 48-49 4.125518061930627E-5 0.0 0.0 0.5283757257817651 8.251036123861254E-5 50-51 4.125518061930627E-5 0.0 0.0 0.6370006163523985 8.251036123861254E-5 52-53 4.125518061930627E-5 0.0 0.0 0.7429645477730866 8.251036123861254E-5 54-55 4.125518061930627E-5 0.0 0.0 0.8649561168643752 8.251036123861254E-5 56-57 8.251036123861254E-5 0.0 0.0 1.008235359155226 8.251036123861254E-5 58-59 8.251036123861254E-5 0.0 0.0 1.159765637569938 8.251036123861254E-5 60-61 8.251036123861254E-5 0.0 0.0 1.3248069876374726 8.251036123861254E-5 62-63 8.251036123861254E-5 0.0 0.0 1.4897039445728397 8.251036123861254E-5 64-65 8.251036123861254E-5 0.0 0.0 1.6622537375130881 8.251036123861254E-5 66-67 8.251036123861254E-5 0.0 0.0 1.8704480065084175 8.251036123861254E-5 68-69 8.251036123861254E-5 0.0 0.0 2.1019514525536547 8.251036123861254E-5 70-71 1.237655418579188E-4 0.0 0.0 2.3433767695378345 8.251036123861254E-5 72-73 1.237655418579188E-4 0.0 0.0 2.5939401090291914 1.650207224772251E-4 74-75 1.650207224772251E-4 0.0 0.0 2.8512899257324236 1.650207224772251E-4 76-77 1.650207224772251E-4 0.0 0.0 3.1266476287759835 1.650207224772251E-4 78-79 1.650207224772251E-4 0.0 0.0 3.437711690645553 1.650207224772251E-4 80-81 1.8564831278687822E-4 0.0 0.0 3.7746840059440467 1.650207224772251E-4 82-83 2.0627590309653135E-4 0.0 0.0 4.12296024073223 1.650207224772251E-4 84-85 2.269034934061845E-4 0.0 0.0 4.475671407436989 1.650207224772251E-4 86-87 2.6815867402549075E-4 0.0 0.0 4.83776812773264 1.650207224772251E-4 88-89 2.887862643351439E-4 0.0 0.0 5.230496819638127 1.650207224772251E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3160 0.0 51.475784 1 GTATCAA 5905 0.0 44.300304 1 ATCAACG 7430 0.0 34.37553 3 TATCAAC 7660 0.0 33.591736 2 TCAACGC 7640 0.0 33.55516 4 CAACGCA 7835 0.0 32.720028 5 AACGCAG 8115 0.0 31.566267 6 TGGTATC 1470 0.0 28.796295 2 ACGCAGA 9505 0.0 26.802265 7 CGCAGAG 9555 0.0 26.562529 8 GTGGTAT 1815 0.0 25.681086 1 GTACATG 7385 0.0 25.504019 1 TACATGG 7435 0.0 24.820738 2 GCAGAGT 10495 0.0 23.27719 9 ACATGGG 7800 0.0 23.049479 3 TAAGGTG 1195 0.0 21.09463 5 GAGTACT 6360 0.0 20.174913 12-13 AGAGTAC 9575 0.0 20.053398 10-11 CAGAGTA 10075 0.0 19.765799 10-11 CATGGGG 5250 0.0 19.477942 4 >>END_MODULE