FastQCFastQC Report
Thu 26 May 2016
SRR938483_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938483_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2423938
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53030.21877622282418116No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45780.1888662168751841No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30760.12690093558498608No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32500.056.157121
GTATCAA59950.041.860241
ATCAACG75950.032.709953
TCAACGC76300.032.5599024
TATCAAC77950.032.0110662
CAACGCA79250.031.3478975
AACGCAG81900.030.437656
TGGTATC14400.027.725142
TATACCG1205.383714E-627.7091225
ACGCAGA95850.025.8591427
CGCAGAG96550.025.6224788
AGGACCG8000.023.7506755
GTGGTAT18500.023.635951
GGACCGA7250.022.92276
GCAGAGT109200.022.7412729
GTACATG75050.022.5453131
TACATGG72450.022.370362
GAGTACT66000.020.75570912-13
ACATGGG73500.020.7454873
AGAGTAC99750.020.46874810-11
Produced by FastQC (version 0.11.5)