##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938483_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2423938 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.776917148870968 31.0 31.0 34.0 30.0 34.0 2 31.998836603906536 31.0 31.0 34.0 30.0 34.0 3 32.06734083132489 33.0 31.0 34.0 30.0 34.0 4 35.74269845185809 37.0 35.0 37.0 33.0 37.0 5 35.63352940545509 37.0 35.0 37.0 33.0 37.0 6 35.631764921380004 37.0 35.0 37.0 33.0 37.0 7 35.569124292783066 37.0 35.0 37.0 33.0 37.0 8 35.54187813384666 37.0 35.0 37.0 33.0 37.0 9 37.12577508170588 39.0 37.0 39.0 33.0 39.0 10-11 37.01677683175065 39.0 37.0 39.0 33.0 39.0 12-13 36.90723153810039 39.0 37.0 39.0 33.0 39.0 14-15 37.95554403619234 40.0 37.0 41.0 33.0 41.0 16-17 37.85690351815929 40.0 37.0 41.0 33.0 41.0 18-19 38.08950517711261 40.0 37.0 41.0 33.0 41.0 20-21 38.05203536559103 40.0 37.0 41.0 33.0 41.0 22-23 37.943594060574156 40.0 37.0 41.0 32.5 41.0 24-25 37.82317782055482 40.0 37.0 41.0 32.0 41.0 26-27 37.62915780024076 40.0 37.0 41.0 32.0 41.0 28-29 37.513485905992646 39.5 36.5 41.0 32.0 41.0 30-31 37.265190570055836 39.0 36.0 41.0 31.0 41.0 32-33 37.079534006232834 39.0 36.0 41.0 31.0 41.0 34-35 36.996973932501575 39.0 36.0 41.0 30.5 41.0 36-37 36.96108749481216 39.0 36.0 41.0 30.5 41.0 38-39 36.799270855937735 39.0 35.0 41.0 30.0 41.0 40-41 36.62492151201887 39.0 35.0 40.5 30.0 41.0 42-43 36.41722519305362 39.0 35.0 40.0 30.0 41.0 44-45 36.13709055264615 38.5 35.0 40.0 29.5 41.0 46-47 35.78724373313179 38.0 35.0 40.0 28.5 41.0 48-49 35.44910719663622 38.0 34.0 40.0 27.5 41.0 50-51 35.07501450119599 38.0 34.0 40.0 26.5 41.0 52-53 34.57256745015755 38.0 33.0 40.0 25.5 41.0 54-55 34.23327102425887 37.5 33.0 40.0 24.0 41.0 56-57 33.89091263885462 37.0 33.0 40.0 23.5 41.0 58-59 33.40883739600601 36.5 32.0 39.0 22.5 41.0 60-61 32.99950101859041 36.0 31.0 39.0 22.0 40.0 62-63 32.50163803694649 35.5 31.0 39.0 20.5 40.0 64-65 31.960474855379964 35.0 30.5 38.5 18.5 40.0 66-67 31.44099292143611 35.0 30.0 38.0 17.5 40.0 68-69 30.925705195429916 34.0 29.0 37.5 13.0 39.5 70-71 30.33250747337597 34.0 29.0 37.0 9.0 39.0 72-73 29.804071102478694 34.0 28.0 36.0 7.5 39.0 74-75 29.191453535527724 33.5 27.5 36.0 7.0 38.5 76-77 27.994009128946367 31.5 26.0 34.5 2.0 36.5 78-79 28.668458516678232 33.0 27.5 35.0 2.0 37.0 80-81 28.280342979069594 33.0 26.5 35.0 2.0 37.0 82-83 27.810368912076136 33.0 26.0 35.0 2.0 36.0 84-85 27.233319911647904 32.0 26.0 35.0 2.0 36.0 86-87 26.58955942767513 32.0 25.0 34.5 2.0 35.0 88-89 25.819911029077478 31.5 23.5 34.0 2.0 35.0 90-91 25.188112278449367 31.0 20.5 34.0 2.0 35.0 92-93 24.329666641638525 31.0 17.5 34.0 2.0 35.0 94-95 23.49065590786563 30.5 4.5 34.0 2.0 35.0 96-97 22.40061152554232 30.0 2.0 34.0 2.0 35.0 98-99 21.010660957499738 29.0 2.0 34.0 2.0 35.0 100-101 17.105412968483517 22.0 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.16689488346541737 1101 2 0.24887002483919218 1101 3 0.28781255538454076 1101 4 0.20634394458205918 1101 5 0.11811105278037104 1101 6 0.14345096342064778 1101 7 0.14663452587767267 1101 8 0.2101196112679915 1101 9 0.2009814506665819 1101 10-11 0.1461218165102025 1101 12-13 0.3003464383461676 1101 14-15 0.40880004506752954 1101 16-17 0.39974104559856016 1101 18-19 0.3292604204230045 1101 20-21 0.3316072198488911 1101 22-23 0.35408752417507827 1101 24-25 0.4922842539204808 1101 26-27 0.2918825638380369 1101 28-29 0.23400795354697834 1101 30-31 0.46893776130983156 1101 32-33 0.3613438051525435 1101 34-35 0.31482546840144465 1101 36-37 0.3369482852767689 1101 38-39 0.3780297336049401 1101 40-41 0.4580562309601035 1101 42-43 0.4040016715669452 1101 44-45 0.43057059600402425 1101 46-47 0.3486075013229808 1101 48-49 0.3749158155921606 1101 50-51 0.41656240779006026 1101 52-53 0.5146773267847564 1101 54-55 0.429950297513237 1101 56-57 0.6374951786306582 1101 58-59 0.7389149736603216 1101 60-61 0.7386949622534402 1101 62-63 0.844004463416546 1101 64-65 0.6857300779277047 1101 66-67 0.44263571468625074 1101 68-69 0.688495134090946 1101 70-71 0.7763184586541776 1101 72-73 0.7121624116126846 1101 74-75 0.7288948191160571 1101 76-77 0.8102398902539854 1101 78-79 0.8222739963053982 1101 80-81 0.775446215258512 1101 82-83 0.9038097800929528 1101 84-85 1.1481514690127987 1101 86-87 1.1928400583526155 1101 88-89 1.0737500097569885 1101 90-91 1.182534764900744 1101 92-93 1.5728742876168482 1101 94-95 1.4467545142466136 1101 96-97 1.6510970218488943 1101 98-99 1.7774836971775194 1101 100-101 1.8448767414434109 1102 1 0.2803308903815598 1102 2 0.26937840609709696 1102 3 0.23576812109529754 1102 4 0.2228378023933999 1102 5 0.110459415425467 1102 6 0.1585903529645094 1102 7 0.19990800418023014 1102 8 0.15989496543993909 1102 9 0.18639529197193383 1102 10-11 0.2954991665571711 1102 12-13 0.37992197008248496 1102 14-15 0.53841085474361 1102 16-17 0.46977182948499063 1102 18-19 0.3768230217144648 1102 20-21 0.2545803392713424 1102 22-23 0.3996879558467086 1102 24-25 0.33386650551698693 1102 26-27 0.19670014354400678 1102 28-29 0.2819163241191873 1102 30-31 0.4386779284840685 1102 32-33 0.5561133674522054 1102 34-35 0.5403809064901779 1102 36-37 0.5252548986968648 1102 38-39 0.5919803020496417 1102 40-41 0.47052334498590653 1102 42-43 0.37863215314682463 1102 44-45 0.5826072433796057 1102 46-47 0.6081312188220878 1102 48-49 0.6463771938266234 1102 50-51 0.6786029467278496 1102 52-53 0.5915076398944663 1102 54-55 0.5292378991173123 1102 56-57 0.6486887249275455 1102 58-59 0.855137975710683 1102 60-61 0.7582742188248517 1102 62-63 0.7105075859499763 1102 64-65 0.7831071384757884 1102 66-67 0.7833793099971267 1102 68-69 0.8979210509858184 1102 70-71 0.8802729982353839 1102 72-73 1.0387080571364606 1102 74-75 0.9284639432265749 1102 76-77 0.8449386427942045 1102 78-79 0.9489306100854691 1102 80-81 1.071271160134362 1102 82-83 1.117855932429233 1102 84-85 0.8839097533605731 1102 86-87 0.7908035057083431 1102 88-89 0.9681221363195327 1102 90-91 1.0352836591021628 1102 92-93 1.5596432603088424 1102 94-95 1.5196634247872893 1102 96-97 1.4857001336587423 1102 98-99 1.5278369502317553 1102 100-101 1.7738826348676575 1103 1 0.23985762839856406 1103 2 0.2503054708814858 1103 3 0.18097657298889658 1103 4 0.19612071319324542 1103 5 0.05141915694878918 1103 6 0.06310635171618628 1103 7 0.21789159237988542 1103 8 0.13184547303085736 1103 9 0.17604961613253067 1103 10-11 0.3169701705407917 1103 12-13 0.40812842098475954 1103 14-15 0.42930303986284457 1103 16-17 0.35633875254779923 1103 18-19 0.4400207527004554 1103 20-21 0.3391886773470887 1103 22-23 0.43455697352519707 1103 24-25 0.4235037569993736 1103 26-27 0.551242210255964 1103 28-29 0.4717896558615209 1103 30-31 0.2703556403780354 1103 32-33 0.4255600658165335 1103 34-35 0.4805975275685128 1103 36-37 0.48279276149127526 1103 38-39 0.438895654609631 1103 40-41 0.5446894456229217 1103 42-43 0.6522177827247759 1103 44-45 0.6293015690088168 1103 46-47 0.6662467990177774 1103 48-49 0.7657260455513324 1103 50-51 0.7758558977310983 1103 52-53 0.7434190455449752 1103 54-55 0.8645465841539348 1103 56-57 0.8364843300897391 1103 58-59 0.8583395618175587 1103 60-61 0.9250269219989065 1103 62-63 0.9355660552834095 1103 64-65 0.72262745129132 1103 66-67 0.8107736154328471 1103 68-69 0.9767960899959718 1103 70-71 0.9577306377999371 1103 72-73 0.9349241641986765 1103 74-75 0.8435382001979193 1103 76-77 0.9162493838953019 1103 78-79 1.1323422084920196 1103 80-81 1.0801034256604005 1103 82-83 1.1554092157071914 1103 84-85 1.1555474454282013 1103 86-87 1.1732893243707956 1103 88-89 1.1849361542733838 1103 90-91 1.3340661752110634 1103 92-93 1.5089212512401282 1103 94-95 1.3067359194176582 1103 96-97 1.3982691127860214 1103 98-99 1.3205950138596059 1103 100-101 1.468045431115339 1104 1 0.20313168365419898 1104 2 0.21924869800826485 1104 3 0.15422981034819827 1104 4 0.12564401865176222 1104 5 0.12880527599892844 1104 6 0.0576054872442171 1104 7 0.05244965313991656 1104 8 0.16017827709563903 1104 9 0.2020285865287761 1104 10-11 0.18376544946531936 1104 12-13 0.2625635150181367 1104 14-15 0.2636529216595349 1104 16-17 0.3250609218431606 1104 18-19 0.2574119880132528 1104 20-21 0.28674998428756737 1104 22-23 0.35906510661859414 1104 24-25 0.4303614896128991 1104 26-27 0.3603265756629668 1104 28-29 0.2902861803313641 1104 30-31 0.3365791328447827 1104 32-33 0.5353289518826543 1104 34-35 0.5020808908937298 1104 36-37 0.4780445168986347 1104 38-39 0.39299607449600416 1104 40-41 0.4877586739493722 1104 42-43 0.5663921237854339 1104 44-45 0.6137636557382891 1104 46-47 0.5751804993106404 1104 48-49 0.6157382187279978 1104 50-51 0.7118375144700977 1104 52-53 0.6386965586884799 1104 54-55 0.7060451198442692 1104 56-57 0.5574917045648817 1104 58-59 0.546100226578865 1104 60-61 0.5510249460629808 1104 62-63 0.3890214732291426 1104 64-65 0.48422443093211953 1104 66-67 0.5228382920497587 1104 68-69 0.6529932954580246 1104 70-71 0.6760777321155977 1104 72-73 0.8040498125526518 1104 74-75 0.8596401373208167 1104 76-77 0.6525353300510481 1104 78-79 0.8483090057115916 1104 80-81 0.7615573263696191 1104 82-83 0.8799247585602963 1104 84-85 0.8682687678938912 1104 86-87 1.0411159712781064 1104 88-89 1.0514971436527247 1104 90-91 1.1206259296304566 1104 92-93 1.3310662002539253 1104 94-95 1.2909672450589582 1104 96-97 1.1995979296943844 1104 98-99 1.2137984999751303 1104 100-101 1.334252163411307 1105 1 0.1643861242983391 1105 2 0.19105165129125368 1105 3 0.16435220815911578 1105 4 0.1476320112969418 1105 5 0.05188787615428225 1105 6 0.14131133710619537 1105 7 0.19605853182341804 1105 8 0.21299113587864582 1105 9 0.2729144244889312 1105 10-11 0.23759141628847402 1105 12-13 0.2780234518756757 1105 14-15 0.37610649484609837 1105 16-17 0.2245080912852515 1105 18-19 0.27925229313284206 1105 20-21 0.2771888658630388 1105 22-23 0.12400659263727931 1105 24-25 0.2913693279884839 1105 26-27 0.20566342344179134 1105 28-29 0.19716356011254987 1105 30-31 0.39143206645835704 1105 32-33 0.3599099000081907 1105 34-35 0.3429634027538171 1105 36-37 0.44857480277627104 1105 38-39 0.38769652178299907 1105 40-41 0.5482318317207273 1105 42-43 0.5279012815808315 1105 44-45 0.4668290109330471 1105 46-47 0.479793726636224 1105 48-49 0.6561941649333392 1105 50-51 0.6733274703825742 1105 52-53 0.5595295330300871 1105 54-55 0.5566422817057983 1105 56-57 0.5868336844226008 1105 58-59 0.6683857482255604 1105 60-61 0.8994638162843032 1105 62-63 0.754165981590809 1105 64-65 0.7326326783244959 1105 66-67 0.6229967326297903 1105 68-69 0.8697771270050794 1105 70-71 0.8811250388761955 1105 72-73 0.7976034899970834 1105 74-75 0.8801610226244634 1105 76-77 0.8411911581731566 1105 78-79 1.111018398070513 1105 80-81 0.918642169972788 1105 82-83 1.0540632118169064 1105 84-85 1.0400403803644949 1105 86-87 0.8596262023752708 1105 88-89 0.8501843526607153 1105 90-91 1.0331179399774335 1105 92-93 1.341202122869337 1105 94-95 1.341871436679721 1105 96-97 1.410251100943789 1105 98-99 1.2594801133874576 1105 100-101 1.379401717693863 1106 1 0.1295386952493658 1106 2 0.17504846859414158 1106 3 0.20054068329919517 1106 4 0.10664334924693009 1106 5 0.06904895281431322 1106 6 0.06097437640221415 1106 7 0.09863303642632104 1106 8 0.08797943358499793 1106 9 -0.03444539918834266 1106 10-11 0.11471390192917141 1106 12-13 0.18343893844193104 1106 14-15 0.1870696446705793 1106 16-17 0.1760413980961104 1106 18-19 0.20001212122145517 1106 20-21 0.4032929456134582 1106 22-23 0.35332008258393444 1106 24-25 0.2960202647029675 1106 26-27 0.22634082490358054 1106 28-29 0.2556643267863805 1106 30-31 0.2528268328168579 1106 32-33 0.3229473171220505 1106 34-35 0.27249084248802546 1106 36-37 0.3666272960387644 1106 38-39 0.1783635239290291 1106 40-41 0.19943028546583008 1106 42-43 0.26139908694125324 1106 44-45 0.3351878061155489 1106 46-47 -0.2579498260739328 1106 48-49 -0.10937127437443905 1106 50-51 0.2593969619924579 1106 52-53 0.1875306505684975 1106 54-55 0.4916477019304608 1106 56-57 0.42235114636871174 1106 58-59 0.2630739280512131 1106 60-61 0.3437296107046137 1106 62-63 0.32556778157920263 1106 64-65 0.4149376666467113 1106 66-67 0.3273050555951187 1106 68-69 8.036856974946716E-4 1106 70-71 0.2568480690717898 1106 72-73 0.7997482978139736 1106 74-75 0.734376748383152 1106 76-77 0.5505521761787584 1106 78-79 0.7246727078230819 1106 80-81 0.32333893556502247 1106 82-83 0.8350311569268172 1106 84-85 1.0685857260375862 1106 86-87 1.158326655517488 1106 88-89 1.329872928689678 1106 90-91 1.1513006493382036 1106 92-93 1.2523293907248316 1106 94-95 1.4508017776024573 1106 96-97 1.486897570772296 1106 98-99 1.2615181505390787 1106 100-101 1.5395524873494253 1107 1 0.0473323775582557 1107 2 0.05878224678080102 1107 3 0.1334294283564148 1107 4 -0.032024778353665795 1107 5 -0.016506646032098615 1107 6 0.00419912301154568 1107 7 -0.011823746619420206 1107 8 0.09174155049735333 1107 9 0.09972860726658439 1107 10-11 0.0034071956540202564 1107 12-13 -0.013098632235916341 1107 14-15 0.0898430867364084 1107 16-17 0.1303181682129093 1107 18-19 0.12236910170000925 1107 20-21 0.09016926902161515 1107 22-23 0.11136389946997127 1107 24-25 0.08246236313630817 1107 26-27 0.08451385174824111 1107 28-29 0.08034883582431718 1107 30-31 0.07950666167131004 1107 32-33 -0.05298551914388128 1107 34-35 0.10158987933711217 1107 36-37 0.12465043933142539 1107 38-39 0.08773565894667712 1107 40-41 0.22674821273135848 1107 42-43 0.03887950069937318 1107 44-45 0.15711819494607937 1107 46-47 0.16844685406503146 1107 48-49 0.16066036417481655 1107 50-51 0.2959815889493953 1107 52-53 0.31667167071491775 1107 54-55 0.1078072161964343 1107 56-57 0.009294658955354862 1107 58-59 0.1145586498681368 1107 60-61 0.2756380298664922 1107 62-63 0.42918733021105737 1107 64-65 0.23860066163019056 1107 66-67 0.3535999580435849 1107 68-69 0.33735690469525537 1107 70-71 0.5609304676538969 1107 72-73 0.4603825159505952 1107 74-75 0.199324750320109 1107 76-77 0.3251387639465122 1107 78-79 0.5690798249461437 1107 80-81 0.5319251559789251 1107 82-83 0.6401547889909232 1107 84-85 0.8385993149495441 1107 86-87 0.8772844201613523 1107 88-89 0.985982969456412 1107 90-91 0.9143674337913446 1107 92-93 1.1986838808305293 1107 94-95 1.3770667033405246 1107 96-97 1.416360224323423 1107 98-99 1.4500020048602167 1107 100-101 1.4765646601202356 1108 1 0.13122543384054097 1108 2 0.07561144413910625 1108 3 0.06300511745893544 1108 4 0.11098134851596342 1108 5 0.07798897529443849 1108 6 0.1730646145667194 1108 7 0.06486212136212544 1108 8 0.04823499709134893 1108 9 0.1433996046569419 1108 10-11 0.17801538436930997 1108 12-13 0.160276226596757 1108 14-15 0.23080345213407583 1108 16-17 0.24819983839371673 1108 18-19 0.2429301125324841 1108 20-21 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-0.955997583167651 2316 76-77 -1.1347335596601766 2316 78-79 -1.3055075924894837 2316 80-81 -1.2827389421002025 2316 82-83 -1.254912730677706 2316 84-85 -1.341767483356744 2316 86-87 -1.5222419511059684 2316 88-89 -1.5488240963780235 2316 90-91 -1.4366384621596566 2316 92-93 -1.663540343785204 2316 94-95 -1.4867532184885732 2316 96-97 -1.6012086435002466 2316 98-99 -1.6616162494682385 2316 100-101 -2.0185055543858823 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 11.0 4 32.0 5 69.0 6 173.0 7 392.0 8 1079.0 9 1877.0 10 3103.0 11 4802.0 12 6704.0 13 9565.0 14 12626.0 15 16249.0 16 19923.0 17 24445.0 18 24269.0 19 22010.0 20 22963.0 21 24685.0 22 26852.0 23 30440.0 24 34713.0 25 40680.0 26 47975.0 27 57531.0 28 68117.0 29 80200.0 30 94553.0 31 111735.0 32 133475.0 33 162298.0 34 203541.0 35 261371.0 36 336137.0 37 363602.0 38 168942.0 39 6796.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.0880661835113 19.147936385873006 15.093235068547855 23.670762362067833 2 16.15986963300022 20.206307097992777 39.339554769288306 24.2942684997187 3 19.608793624259366 23.518959643357213 29.513914959871084 27.358331772512333 4 13.71990537711773 16.86412771283754 35.72562499535879 33.69034191468593 5 14.227340267459137 36.82066204391613 34.07850421000495 14.873493478619778 6 33.46623013148862 35.62004112319797 16.754196480435127 14.15953226487828 7 29.480415753208213 31.65629648943166 20.194080871705463 18.669206885654667 8 26.334501955083013 35.74377727483128 19.154079023473372 18.76764174661233 9 26.036722061372856 16.965285415716078 19.59010502743882 37.407887495472245 10-11 25.313188703671464 26.310635833094743 27.079364241164583 21.296811222069213 12-13 26.11341544214415 23.33172300611649 27.45196040492785 23.10290114681151 14-15 22.743568523617352 25.147260367220614 25.379052599530187 26.730118509631843 16-17 22.6902462026669 28.20730150688673 26.256735939615616 22.845716350830756 18-19 22.67902479353845 26.7672069170086 28.309985651448184 22.243782638004767 20-21 23.0067146931976 26.07088135092564 28.567665509596367 22.354738446280393 22-23 22.92507069075199 25.856148135802155 28.448479292787194 22.770301880658664 24-25 22.949487248038604 26.130216223887935 28.353784825710544 22.566511702362916 26-27 22.43189627271319 26.519780460742 28.233881466375703 22.814441800169107 28-29 22.60345265033922 26.759030254524152 28.19945666859879 22.438060426537838 30-31 22.640012937822128 26.34160801121828 28.164846666592407 22.853532384367185 32-33 22.479089708184162 26.285332142977964 28.154427981712317 23.081150167125564 34-35 23.09257797212946 26.445632770626204 27.886505199448525 22.575284057795812 36-37 23.185355166993833 26.015339077715876 27.970811516110118 22.828494239180177 38-39 22.66797835589011 26.352947680473992 27.97697791506187 23.002096048574032 40-41 23.080972478351093 26.16033598607209 27.9968934490142 22.76179808656262 42-43 22.809210618598637 26.203784152633485 27.84708762497432 23.13991760379356 44-45 22.853202081820953 26.44782750319908 27.938558679992337 22.760411734987635 46-47 23.125055178804075 26.69410273695119 27.32054202706505 22.860300057179682 48-49 22.980744556997745 26.552968764052547 27.449856390716263 23.016430288233444 50-51 23.15176048399257 26.814606001060675 27.263197261132426 22.770436253814324 52-53 23.27611102264167 26.625041564594476 27.191454566907243 22.90739284585662 54-55 22.87069184066115 26.742958207700923 27.37394454853504 23.012405403102886 56-57 22.964077464027547 26.896129356443936 27.263073560462352 22.87671961906616 58-59 22.9600018156609 26.499797801399712 27.300483626039878 23.23971675689951 60-61 22.766650797173853 26.764277799184633 27.40800300997798 23.061068393663533 62-63 22.706620742694277 26.789875546474917 27.58134616431958 22.922157546511222 64-65 22.540723776058584 26.67523122555372 27.431269827548682 23.352775170839017 66-67 22.51925254474382 26.77099585129863 27.274570756595974 23.43518084736158 68-69 22.81407597092807 26.462901267832635 27.5425148524493 23.180507908789995 70-71 22.77567261498349 26.51232396908333 27.203307844476026 23.508695571457157 72-73 22.74124043317159 26.60060467780599 27.18702704754314 23.47112784147928 74-75 22.73467131665837 26.917827565581614 26.947311258458505 23.400189859301506 76-77 22.816986170529148 26.66265184318991 27.195737297634427 23.324624688646516 78-79 22.848424609100388 26.720041862683054 27.209258321931213 23.222275206285346 80-81 22.897059445562654 26.861046087565654 27.004344340853827 23.237550126017865 82-83 22.814689138694252 26.882489036508655 26.97625546730102 23.326566357496073 84-85 22.825846111187445 26.833452374615696 27.002627160640536 23.338074353556323 86-87 22.935257830130272 26.82377779249358 26.848863420924673 23.392100956451472 88-89 22.83206723875767 26.921310131750225 26.655703825742442 23.590918803749663 90-91 22.737721366774707 26.865602660476274 26.855121196058885 23.541554776690134 92-93 22.709607321168523 26.63129934070033 26.981520049485646 23.677573288645494 94-95 22.55966501303926 26.523068009459166 27.072823638088963 23.844443339412614 96-97 22.734098465052252 26.34675502062232 27.01116372535242 23.907982788973005 98-99 22.513757877860755 26.588801673565555 26.74997069278944 24.147469755784254 100-101 22.745749235587567 26.254918574422646 27.02047599979122 23.978856190198563 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 309.0 1 216.5 2 199.0 3 283.5 4 244.0 5 220.5 6 360.0 7 800.5 8 1298.5 9 1532.5 10 1419.0 11 1203.0 12 1249.0 13 1376.0 14 1517.5 15 1691.0 16 1842.0 17 2048.0 18 2273.5 19 2499.5 20 2851.5 21 3351.5 22 4139.5 23 5076.0 24 6332.5 25 8086.5 26 10400.0 27 13416.0 28 16442.5 29 19557.5 30 23263.0 31 28537.0 32 35730.0 33 39815.5 34 41586.5 35 45138.0 36 50377.0 37 55935.0 38 59999.0 39 63563.0 40 67537.5 41 72884.5 42 77500.0 43 80881.5 44 84360.0 45 88399.5 46 90965.0 47 93516.0 48 94974.0 49 94495.5 50 94551.0 51 92533.5 52 90454.5 53 90585.0 54 91250.5 55 85967.0 56 76763.5 57 69439.0 58 63720.0 59 56112.5 60 45567.5 61 37856.0 62 32143.5 63 24659.0 64 17750.5 65 13043.5 66 9718.0 67 7633.5 68 5837.0 69 4342.0 70 3323.5 71 2392.5 72 1777.5 73 1456.5 74 1149.0 75 855.5 76 612.0 77 350.0 78 201.5 79 149.0 80 96.0 81 52.0 82 30.5 83 15.5 84 7.0 85 2.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.53806986812369E-4 2 0.05111516878732046 3 0.0 4 0.0 5 0.04694839554477054 6 8.251036123861254E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.006353297815373165 26-27 0.0017946003569398227 28-29 1.237655418579188E-4 30-31 0.001526441682914332 32-33 4.950621674316752E-4 34-35 0.011035760815664427 36-37 0.024134280662294165 38-39 0.0037748490266665234 40-41 0.0011551450573405754 42-43 0.0016502072247722506 44-45 0.008024132630455068 46-47 0.0 48-49 0.0 50-51 6.807104802185535E-4 52-53 0.0 54-55 0.0014233037313660663 56-57 0.0 58-59 0.023845494397959025 60-61 0.0 62-63 0.009158650097485992 64-65 0.01586261694812326 66-67 0.0069102427537338 68-69 0.017822238027540308 70-71 1.237655418579188E-4 72-73 1.0313795154826566E-4 74-75 0.02328854945959839 76-77 0.05154834818382318 78-79 0.03207590293151062 80-81 0.027661598605244853 82-83 0.036964641834898414 84-85 0.016873368873296265 86-87 0.009880615758323852 88-89 0.08748161050323894 90-91 0.02526879812932509 92-93 0.02512440499715752 94-95 0.028032895230818606 96-97 0.03946058026236645 98-99 0.05470436950120012 100-101 0.01421240972335101 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2423938.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.01736520502645 #Duplication Level Percentage of deduplicated Percentage of total 1 78.90734334951125 45.77996156467009 2 12.335147710309453 14.313055391339388 3 3.749254137011096 6.525655395402871 4 1.5925663253122095 3.6958600843546163 5 0.8645593965365905 2.5079729125150325 6 0.532622095369777 1.8540798374000869 7 0.3460103049451987 1.4052224358695713 8 0.2608585056172916 1.2107458549788683 9 0.19287919582239327 1.0071308470031644 >10 1.1010902225044967 11.76833280229539 >50 0.0755749863166611 2.999661445516865 >100 0.03753381105092827 3.956842305185616 >500 0.0027074760675215126 1.0778624657127958 >1k 0.0017812342549469356 1.678406507969685 >5k 7.124937019787742E-5 0.21921014978605619 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5303 0.21877622282418116 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4578 0.1888662168751841 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3076 0.12690093558498608 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 4.125518061930627E-5 7 4.125518061930627E-5 0.0 0.0 4.125518061930627E-5 4.125518061930627E-5 8 4.125518061930627E-5 0.0 0.0 8.251036123861254E-5 4.125518061930627E-5 9 4.125518061930627E-5 0.0 0.0 8.251036123861254E-5 4.125518061930627E-5 10-11 4.125518061930627E-5 0.0 0.0 8.251036123861254E-5 4.125518061930627E-5 12-13 4.125518061930627E-5 0.0 0.0 1.650207224772251E-4 4.125518061930627E-5 14-15 4.125518061930627E-5 0.0 0.0 1.650207224772251E-4 4.125518061930627E-5 16-17 4.125518061930627E-5 0.0 0.0 2.269034934061845E-4 1.237655418579188E-4 18-19 4.125518061930627E-5 0.0 0.0 2.6815867402549075E-4 1.237655418579188E-4 20-21 4.125518061930627E-5 0.0 0.0 3.300414449544502E-4 1.237655418579188E-4 22-23 4.125518061930627E-5 0.0 0.0 4.950621674316752E-4 1.237655418579188E-4 24-25 4.125518061930627E-5 0.0 0.0 8.457312026957785E-4 1.237655418579188E-4 26-27 4.125518061930627E-5 0.0 0.0 9.901243348633504E-4 1.237655418579188E-4 28-29 4.125518061930627E-5 0.0 0.0 0.002454683246848723 1.237655418579188E-4 30-31 4.125518061930627E-5 0.0 0.0 0.008849236242841195 1.237655418579188E-4 32-33 4.125518061930627E-5 0.0 0.0 0.023391687411146653 1.237655418579188E-4 34-35 4.125518061930627E-5 0.0 0.0 0.044163670852967365 1.650207224772251E-4 36-37 4.125518061930627E-5 0.0 0.0 0.08057136774950514 1.650207224772251E-4 38-39 4.125518061930627E-5 0.0 0.0 0.13498695098637012 1.650207224772251E-4 40-41 4.125518061930627E-5 0.0 0.0 0.19501323878746074 1.650207224772251E-4 42-43 8.251036123861253E-5 0.0 0.0 0.2575973477869483 1.650207224772251E-4 44-45 1.237655418579188E-4 0.0 0.0 0.33637411517951366 1.650207224772251E-4 46-47 2.0627590309653135E-4 0.0 0.0 0.43014714072719684 1.650207224772251E-4 48-49 2.0627590309653135E-4 0.0 0.0 0.533140699143295 1.650207224772251E-4 50-51 2.0627590309653135E-4 0.0 0.0 0.642446300194147 1.650207224772251E-4 52-53 2.0627590309653135E-4 0.0 0.0 0.7493797283593888 1.650207224772251E-4 54-55 2.475310837158376E-4 0.0 0.0 0.8722170286533731 1.650207224772251E-4 56-57 2.475310837158376E-4 0.0 0.0 1.0158675675697975 1.650207224772251E-4 58-59 2.475310837158376E-4 0.0 0.0 1.1683673427290633 1.650207224772251E-4 60-61 2.475310837158376E-4 0.0 0.0 1.3339656377349587 1.650207224772251E-4 62-63 2.475310837158376E-4 0.0 0.0 1.4996876982827119 1.650207224772251E-4 64-65 2.475310837158376E-4 0.0 0.0 1.6735782845930878 1.650207224772251E-4 66-67 2.475310837158376E-4 0.0 0.0 1.883608409125976 1.650207224772251E-4 68-69 2.475310837158376E-4 0.0 0.0 2.116720807215366 1.650207224772251E-4 70-71 2.475310837158376E-4 0.0 0.0 2.360250138411131 1.650207224772251E-4 72-73 2.475310837158376E-4 0.0 0.0 2.6155784512640174 1.650207224772251E-4 74-75 2.475310837158376E-4 0.0 0.0 2.879178427831075 1.650207224772251E-4 76-77 2.475310837158376E-4 0.0 0.0 3.161611394350846 1.650207224772251E-4 78-79 2.475310837158376E-4 0.0 0.0 3.48245293402719 1.650207224772251E-4 80-81 2.887862643351439E-4 0.0 0.0 3.8302753618285617 1.650207224772251E-4 82-83 2.887862643351439E-4 0.0 0.0 4.191051916344396 2.0627590309653135E-4 84-85 3.09413854644797E-4 0.0 0.0 4.557005996027952 2.0627590309653135E-4 86-87 3.300414449544502E-4 0.0 0.0 4.934614664236461 2.0627590309653135E-4 88-89 3.300414449544502E-4 0.0 0.0 5.346444504768686 2.0627590309653135E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3250 0.0 56.15712 1 GTATCAA 5995 0.0 41.86024 1 ATCAACG 7595 0.0 32.70995 3 TCAACGC 7630 0.0 32.559902 4 TATCAAC 7795 0.0 32.011066 2 CAACGCA 7925 0.0 31.347897 5 AACGCAG 8190 0.0 30.43765 6 TGGTATC 1440 0.0 27.72514 2 TATACCG 120 5.383714E-6 27.709122 5 ACGCAGA 9585 0.0 25.859142 7 CGCAGAG 9655 0.0 25.622478 8 AGGACCG 800 0.0 23.750675 5 GTGGTAT 1850 0.0 23.63595 1 GGACCGA 725 0.0 22.9227 6 GCAGAGT 10920 0.0 22.741272 9 GTACATG 7505 0.0 22.545313 1 TACATGG 7245 0.0 22.37036 2 GAGTACT 6600 0.0 20.755709 12-13 ACATGGG 7350 0.0 20.745487 3 AGAGTAC 9975 0.0 20.468748 10-11 >>END_MODULE