FastQCFastQC Report
Thu 26 May 2016
SRR938482_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938482_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2440465
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55180.22610445140577717No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42860.17562226870698822No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29230.11977225651668842No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32750.053.212571
GTATCAA58400.042.315451
ATCAACG75950.031.7310583
TCAACGC77500.031.0964364
TATCAAC78450.030.658652
CAACGCA78900.030.5427825
TGGTATC16200.029.92822
AACGCAG82800.029.1567926
GTGGTAT17550.029.030271
GTACATG69450.025.3672811
TACATGG69750.025.1465052
ACGCAGA95700.025.02287
CGCAGAG96750.024.6039078
TAAGGTG10450.023.6518275
ACATGGG72600.022.5229993
GCAGAGT105300.021.7001539
GTAAGGT10500.020.824424
GAGTACT65650.020.76665512-13
AGAGTAC96500.019.91241610-11
CATGGGG48750.019.7936444
Produced by FastQC (version 0.11.5)