FastQCFastQC Report
Thu 26 May 2016
SRR938482_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938482_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2440465
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53510.219261493199042No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47070.1928730795155841No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31190.1278035128551321No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35600.053.308371
GTATCAA60300.039.991081
ATCAACG76300.031.4616153
TATCAAC78450.030.4957032
TCAACGC78750.030.4815584
CAACGCA80200.029.930465
AACGCAG83850.028.8885176
TGGTATC17150.025.2371832
ACGCAGA98300.024.4486647
CGCAGAG98200.024.3284598
GTGGTAT19650.023.7211671
GTACATG74350.023.4138371
TACATGG75400.022.771912
ACATGGG73300.022.243563
TAAGGTG12800.021.90985
GTAAGGT12400.021.8499034
CCGTCCG2005.39545E-721.3732539
GCAGAGT110200.021.0758639
GAGTACT65900.020.3245912-13
ATACCGT1951.0341546E-519.4855886
Produced by FastQC (version 0.11.5)