FastQCFastQC Report
Thu 26 May 2016
SRR938481_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938481_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2451581
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54420.22197920443991043No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45030.18367739022288068No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29130.11882128308222327No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30700.052.6350861
GTATCAA57650.045.806221
ATCAACG76000.034.2058453
TATCAAC76600.034.0622
TCAACGC77200.033.797274
CAACGCA79650.032.817355
AACGCAG82850.031.6052386
ACGCAGA94550.027.3363087
CGCAGAG95050.026.9425778
GTACATG72800.026.321381
TACATGG74700.026.0213172
ACATGGG77000.024.3182373
GCAGAGT105400.023.2140739
TGGTATC14850.022.082552
AGAGTAC95450.020.65292510-11
GAGTACT63250.020.46454612-13
CATGGGG53050.020.2464854
AGTACTT66850.019.61118512-13
GTGGTAT18500.019.5819471
CAGAGTA101400.019.5815810-11
Produced by FastQC (version 0.11.5)