##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938481_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2451581 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.658475897798198 31.0 31.0 34.0 27.0 34.0 2 30.82379085169937 31.0 31.0 34.0 27.0 34.0 3 30.86567158091044 31.0 31.0 34.0 27.0 34.0 4 34.51797309572884 37.0 35.0 37.0 30.0 37.0 5 34.503536289439346 37.0 35.0 37.0 30.0 37.0 6 34.48759229248391 37.0 35.0 37.0 30.0 37.0 7 34.37912881524208 37.0 35.0 37.0 30.0 37.0 8 34.32587501697884 37.0 35.0 37.0 30.0 37.0 9 35.743723743983985 38.0 35.0 39.0 30.0 39.0 10-11 35.580605535774666 38.0 35.0 39.0 30.0 39.0 12-13 35.37570347461495 38.0 35.0 39.0 29.0 39.0 14-15 36.27989611601656 39.0 36.0 40.5 28.5 41.0 16-17 36.06373846101761 38.5 35.0 40.0 27.0 41.0 18-19 35.88898979882778 38.5 34.5 40.0 27.0 41.0 20-21 35.84701953555685 38.5 34.5 40.0 27.0 41.0 22-23 35.938057930780175 39.0 35.0 40.0 27.0 41.0 24-25 35.82418447524271 39.0 35.0 40.5 27.0 41.0 26-27 35.59338218888138 38.5 34.0 40.0 27.0 41.0 28-29 35.36437343901751 38.0 34.0 40.0 26.0 41.0 30-31 35.0687493091193 38.0 34.0 40.0 25.0 41.0 32-33 34.74492725306649 38.0 33.5 40.0 24.5 41.0 34-35 34.445111542306776 38.0 33.0 40.0 24.0 41.0 36-37 34.114224045626074 38.0 33.0 40.0 23.5 41.0 38-39 33.743922187355835 38.0 32.5 40.0 21.5 41.0 40-41 33.59006840891653 38.0 32.0 40.0 20.0 41.0 42-43 33.32473289685309 38.0 31.5 40.0 18.5 41.0 44-45 32.98073895987936 37.0 31.0 40.0 16.0 41.0 46-47 32.55646539926684 37.0 31.0 40.0 14.0 41.0 48-49 32.13799482864323 37.0 30.0 40.0 9.0 41.0 50-51 31.35414024664084 36.0 29.5 38.5 8.5 40.5 52-53 31.363210312039456 36.0 30.0 39.0 7.5 40.0 54-55 31.385234671014338 36.0 30.0 39.0 4.5 40.5 56-57 30.992346979357404 36.0 29.0 39.0 2.0 41.0 58-59 30.496025014062354 35.0 28.0 39.0 2.0 40.0 60-61 29.939743577715767 35.0 27.5 39.0 2.0 40.0 62-63 29.29651824679666 34.0 26.0 38.0 2.0 40.0 64-65 28.7391108023761 34.0 26.0 38.0 2.0 40.0 66-67 28.12544721141174 34.0 25.0 37.5 2.0 40.0 68-69 27.451392387198304 33.5 22.5 37.0 2.0 39.0 70-71 26.7668910388847 33.0 21.5 36.0 2.0 39.0 72-73 26.080247807435285 32.0 19.5 36.0 2.0 39.0 74-75 25.32418263969251 31.5 16.0 35.0 2.0 38.0 76-77 24.538214727557442 31.0 9.0 35.0 2.0 37.0 78-79 23.704701782237667 30.5 4.5 35.0 2.0 36.5 80-81 22.9045422117401 30.0 2.0 34.0 2.0 36.0 82-83 22.142132770648818 29.0 2.0 34.0 2.0 35.5 84-85 21.39438937567227 29.0 2.0 34.0 2.0 35.0 86-87 20.651732902155793 27.5 2.0 34.0 2.0 35.0 88-89 19.857643292226527 26.5 2.0 34.0 2.0 35.0 90-91 19.03500761345434 25.5 2.0 33.0 2.0 35.0 92-93 18.169742504938647 24.5 2.0 33.0 2.0 35.0 94-95 17.272834754389105 19.5 2.0 32.0 2.0 34.5 96-97 16.228522736960354 9.0 2.0 32.0 2.0 34.0 98-99 15.02849426553722 2.0 2.0 31.5 2.0 34.0 100-101 12.545287714336178 2.0 2.0 28.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7420197962986386 1101 2 0.7451338812252963 1101 3 0.8369590699519485 1101 4 0.7617895628237861 1101 5 0.7818506094092754 1101 6 0.7548796161398386 1101 7 0.8133063764583497 1101 8 0.7244829430662136 1101 9 0.9780023596812626 1101 10-11 0.9738587737147526 1101 12-13 1.0722295451434363 1101 14-15 1.1696366977043482 1101 16-17 1.3317257858044371 1101 18-19 1.3154553702057754 1101 20-21 1.3608944561306089 1101 22-23 1.3556221865059115 1101 24-25 1.4367528746699278 1101 26-27 1.4849646199516684 1101 28-29 1.4933724246940798 1101 30-31 1.5758083671121668 1101 32-33 1.4357251315255795 1101 34-35 1.494241894912406 1101 36-37 1.7365938806144001 1101 38-39 1.9927319318801935 1101 40-41 1.9497329431656496 1101 42-43 2.1907536289389 1101 44-45 2.3163426905280886 1101 46-47 2.3562339968951562 1101 48-49 2.5229795415549603 1101 50-51 2.4757779592498856 1101 52-53 2.4856171740112494 1101 54-55 2.850668738672365 1101 56-57 2.9910042507825914 1101 58-59 3.221482011005797 1101 60-61 3.367171860260431 1101 62-63 3.231878119749819 1101 64-65 3.509406193460304 1101 66-67 3.5956873798294673 1101 68-69 3.8922023403536947 1101 70-71 4.03794691227451 1101 72-73 4.333309820855092 1101 74-75 4.666410189889167 1101 76-77 4.745022951899276 1101 78-79 5.180968320686802 1101 80-81 5.583861366057363 1101 82-83 5.725219260880408 1101 84-85 5.705619726566166 1101 86-87 5.8187513450088275 1101 88-89 6.340560375138857 1101 90-91 6.35011689261648 1101 92-93 6.430520208653718 1101 94-95 6.564586197681656 1101 96-97 6.521296353469626 1101 98-99 6.813814330571377 1101 100-101 6.575812851235627 1102 1 0.8243439195001301 1102 2 0.8648365447963542 1102 3 0.7904436046927152 1102 4 0.7922430740848725 1102 5 0.7676885501325614 1102 6 0.8393249979431445 1102 7 0.8403290380772219 1102 8 0.9448010098448947 1102 9 1.1490748066759409 1102 10-11 1.1346078783820985 1102 12-13 1.169063460646342 1102 14-15 1.470306076514312 1102 16-17 1.568877094117262 1102 18-19 1.5983618953854872 1102 20-21 1.493976561794966 1102 22-23 1.348232932356936 1102 24-25 1.2940181999063398 1102 26-27 1.4596033494508163 1102 28-29 1.4363619293735113 1102 30-31 1.512548844979257 1102 32-33 1.498283087029698 1102 34-35 1.4538546068930174 1102 36-37 1.6756610032557901 1102 38-39 1.6537265122416756 1102 40-41 1.9553562248357892 1102 42-43 2.010490223351468 1102 44-45 2.1006511869502447 1102 46-47 2.1410622045797894 1102 48-49 2.078219125832433 1102 50-51 2.33636170916564 1102 52-53 2.335949917040903 1102 54-55 2.5174751376572253 1102 56-57 2.468298475335043 1102 58-59 2.6650914507518237 1102 60-61 2.634264231166366 1102 62-63 3.0463292883884243 1102 64-65 3.0807902348094522 1102 66-67 3.0971509378732875 1102 68-69 3.388808965699102 1102 70-71 3.5429572309687742 1102 72-73 3.499322854016679 1102 74-75 3.779842619871946 1102 76-77 4.148980659196965 1102 78-79 4.47832043683254 1102 80-81 4.675303240899037 1102 82-83 5.053206743653533 1102 84-85 4.929456956136196 1102 86-87 5.24521873770254 1102 88-89 5.574320342323546 1102 90-91 5.840258065833421 1102 92-93 5.8911377732094685 1102 94-95 6.122460651244044 1102 96-97 5.792609163291903 1102 98-99 6.309659255005533 1102 100-101 5.938132942244652 1103 1 0.8566776874271191 1103 2 0.8199864796073086 1103 3 0.6795135742711587 1103 4 0.8319504476905522 1103 5 0.7669207680088448 1103 6 0.8313502043751342 1103 7 0.9512445820436142 1103 8 0.8560497422800708 1103 9 1.0904046632600313 1103 10-11 1.0931295356325705 1103 12-13 1.2160683860411012 1103 14-15 1.390844248323667 1103 16-17 1.417587799607631 1103 18-19 1.5088645753796825 1103 20-21 1.554913835444097 1103 22-23 1.5400444635752422 1103 24-25 1.600656617985429 1103 26-27 1.8625984240560598 1103 28-29 1.832906909816792 1103 30-31 1.865160028522645 1103 32-33 1.9110347891881716 1103 34-35 2.0102721065308486 1103 36-37 2.033375040630851 1103 38-39 2.2970808509349965 1103 40-41 2.35650427640757 1103 42-43 2.663467189887754 1103 44-45 2.465919910693543 1103 46-47 2.578770447372783 1103 48-49 2.6389354810403134 1103 50-51 2.6242148165102783 1103 52-53 3.004492579651359 1103 54-55 3.1294988954436924 1103 56-57 3.299520407828151 1103 58-59 3.413718859124998 1103 60-61 3.6311967242852923 1103 62-63 3.7622462810003334 1103 64-65 3.857981311153072 1103 66-67 4.034739667411166 1103 68-69 3.962414644389522 1103 70-71 4.3245847842065 1103 72-73 4.543810757826616 1103 74-75 4.808228828766627 1103 76-77 4.993903156661833 1103 78-79 5.024887146958569 1103 80-81 5.417904283923807 1103 82-83 5.706592952548217 1103 84-85 5.909512728988577 1103 86-87 5.957992169543768 1103 88-89 6.227688443149276 1103 90-91 6.471092485650896 1103 92-93 6.50493974336737 1103 94-95 6.670443412362399 1103 96-97 6.6877562008118225 1103 98-99 6.683406176633811 1103 100-101 6.501315616444275 1104 1 0.6958111030311223 1104 2 0.7254574791959527 1104 3 0.6710465510982537 1104 4 0.6452737257197114 1104 5 0.6109675708791826 1104 6 0.6422030769927076 1104 7 0.6247008643234295 1104 8 0.6171197180559318 1104 9 0.8038284943309222 1104 10-11 0.9238450619363334 1104 12-13 0.7834080724984318 1104 14-15 1.0591711828270718 1104 16-17 1.184581263659915 1104 18-19 1.216387771138173 1104 20-21 1.3150285573235294 1104 22-23 1.35294838971447 1104 24-25 1.4418090929614351 1104 26-27 1.3852971305782944 1104 28-29 1.2518223081439572 1104 30-31 1.4466091349717516 1104 32-33 1.52020682630139 1104 34-35 1.5461581159462696 1104 36-37 1.6595508256301699 1104 38-39 1.7570436006036303 1104 40-41 1.946630653298655 1104 42-43 2.081539313842235 1104 44-45 2.1117286770905395 1104 46-47 2.1803975784116787 1104 48-49 2.3356252292006516 1104 50-51 2.337700350584047 1104 52-53 2.470859872489239 1104 54-55 2.6913207337361236 1104 56-57 2.850047168649038 1104 58-59 3.024186659298678 1104 60-61 3.179269076769412 1104 62-63 3.285045540564301 1104 64-65 3.383789163431505 1104 66-67 3.5713832525841624 1104 68-69 3.8222208512545564 1104 70-71 3.833315759113944 1104 72-73 4.063770536609923 1104 74-75 4.101484919081539 1104 76-77 4.212217522035022 1104 78-79 4.514610169034533 1104 80-81 4.944378300103708 1104 82-83 5.108771982668426 1104 84-85 5.2611181497116455 1104 86-87 5.2715314080830105 1104 88-89 5.8304993577248965 1104 90-91 5.889555395290273 1104 92-93 6.004803768231394 1104 94-95 5.970369140229305 1104 96-97 5.953028836672697 1104 98-99 5.90415781062074 1104 100-101 5.638389979989222 1105 1 0.7004434800620523 1105 2 0.6075967691562063 1105 3 0.525594929641688 1105 4 0.5873858756623278 1105 5 0.5381060268354645 1105 6 0.559513909275509 1105 7 0.596407030437085 1105 8 0.5393430330854798 1105 9 0.6984055745727389 1105 10-11 0.7514642497427317 1105 12-13 0.8880582925625049 1105 14-15 1.0307414494890423 1105 16-17 0.9818367260536434 1105 18-19 1.050739048958519 1105 20-21 1.060852556568605 1105 22-23 0.9963194038566456 1105 24-25 1.0168386319865093 1105 26-27 1.192060404366181 1105 28-29 1.2677501226083123 1105 30-31 1.2000646858828787 1105 32-33 1.3428506487907939 1105 34-35 1.453662673063583 1105 36-37 1.4263175450838546 1105 38-39 1.478330937233551 1105 40-41 1.4148144817290813 1105 42-43 1.7237484541270192 1105 44-45 1.6984209807955821 1105 46-47 1.8016709762103105 1105 48-49 1.8768692175604826 1105 50-51 1.8305815784505377 1105 52-53 1.8858724589714662 1105 54-55 1.9996292407759775 1105 56-57 2.025953308978341 1105 58-59 2.1956403481547255 1105 60-61 2.4699830904949245 1105 62-63 2.6588195381147877 1105 64-65 2.8098843668121845 1105 66-67 3.105198292433606 1105 68-69 3.0829706141781266 1105 70-71 3.4811431289312402 1105 72-73 3.7114598472043028 1105 74-75 3.750847886248369 1105 76-77 3.9374034949521572 1105 78-79 4.1891243368015765 1105 80-81 4.552380900468279 1105 82-83 4.618848727647286 1105 84-85 4.761007419901688 1105 86-87 4.980769464049658 1105 88-89 5.148315177750163 1105 90-91 5.211987643266568 1105 92-93 5.283865982269379 1105 94-95 5.500183619202769 1105 96-97 5.820551376377598 1105 98-99 5.899116655349138 1105 100-101 5.4672411511501 1106 1 0.28775594684413974 1106 2 0.3371062490886416 1106 3 0.21037467842216273 1106 4 0.3757002736879329 1106 5 0.35282307597955764 1106 6 0.12054672989087578 1106 7 0.2749326023114378 1106 8 0.3236943036542712 1106 9 0.3277094323237577 1106 10-11 0.35955468666684 1106 12-13 0.44105237780033235 1106 14-15 0.7876836647884886 1106 16-17 0.6596999129738137 1106 18-19 0.8690419884479823 1106 20-21 0.8912501059416087 1106 22-23 0.7556552823619569 1106 24-25 0.7928933172253849 1106 26-27 0.8830907122600706 1106 28-29 0.7740891531977354 1106 30-31 1.1330812540578776 1106 32-33 1.2108660066496881 1106 34-35 1.1922360392684013 1106 36-37 1.2009040642673625 1106 38-39 1.2895959543621558 1106 40-41 1.3532817479407413 1106 42-43 1.2373730614320664 1106 44-45 1.5473172909101436 1106 46-47 1.7571648904252086 1106 48-49 1.9135342310055137 1106 50-51 1.2699917729956987 1106 52-53 1.7955457130904868 1106 54-55 1.640011437552328 1106 56-57 1.863131588540309 1106 58-59 1.5500008408059998 1106 60-61 2.5053430674462938 1106 62-63 2.9593272130975556 1106 64-65 2.850064888090067 1106 66-67 2.8174532034227475 1106 68-69 2.2657641947667635 1106 70-71 2.847458124221532 1106 72-73 3.246673796886725 1106 74-75 3.587578546186375 1106 76-77 3.5089874435295094 1106 78-79 3.9323393310394223 1106 80-81 4.462500458650279 1106 82-83 4.581480546857634 1106 84-85 4.844231123153225 1106 86-87 5.001529016337944 1106 88-89 5.109750165357578 1106 90-91 5.592107477716372 1106 92-93 5.830798620284877 1106 94-95 5.516225240921102 1106 96-97 5.639575572175168 1106 98-99 5.362857163576654 1106 100-101 4.679747992067201 1107 1 0.44379763662136895 1107 2 0.40099981611599844 1107 3 0.3471030797089618 1107 4 0.4781208321736301 1107 5 0.3751403176370687 1107 6 0.4156123302781225 1107 7 0.41874544203673736 1107 8 0.36527945305776655 1107 9 0.3124615021899402 1107 10-11 0.4126722177526574 1107 12-13 0.5059651207133697 1107 14-15 0.6864417450821563 1107 16-17 0.8678689512045707 1107 18-19 0.8126349836779596 1107 20-21 0.9567641437185586 1107 22-23 0.9866382292866547 1107 24-25 0.9312938728873874 1107 26-27 0.9166655299807474 1107 28-29 1.0342475067843253 1107 30-31 1.0345697424757034 1107 32-33 1.259272134058648 1107 34-35 1.326203148313283 1107 36-37 1.452913422785329 1107 38-39 1.4819605180515012 1107 40-41 1.57811623428114 1107 42-43 1.6252983798536178 1107 44-45 1.7678859872726207 1107 46-47 1.7239455552026897 1107 48-49 1.8525015417425976 1107 50-51 2.0809942084289865 1107 52-53 2.2154581534652316 1107 54-55 2.2126691608508047 1107 56-57 2.256489990533037 1107 58-59 2.446696618379846 1107 60-61 2.5334280756652596 1107 62-63 2.872498722057731 1107 64-65 3.10191604429059 1107 66-67 3.273963953616878 1107 68-69 3.483287338946429 1107 70-71 3.488915577252463 1107 72-73 3.8113741327042305 1107 74-75 3.7277609263560016 1107 76-77 4.298674795411802 1107 78-79 4.293801372567842 1107 80-81 4.5668276386908175 1107 82-83 4.696593217161485 1107 84-85 4.858512821603224 1107 86-87 5.104871056051142 1107 88-89 5.203874241355198 1107 90-91 5.52591903165391 1107 92-93 5.488610789383618 1107 94-95 5.527369995411274 1107 96-97 5.689000875469812 1107 98-99 5.630923111882945 1107 100-101 5.25402492553903 1108 1 0.3757960658162496 1108 2 0.4279055582013278 1108 3 0.3379001191976556 1108 4 0.43932116506902474 1108 5 0.43421934306537224 1108 6 0.3521544453888339 1108 7 0.3977104039555215 1108 8 0.5123568908680554 1108 9 0.45181581481858757 1108 10-11 0.4281525442591487 1108 12-13 0.5828671699655601 1108 14-15 0.5057781399643062 1108 16-17 0.7887633613960645 1108 18-19 0.8908727576981832 1108 20-21 0.6996898162945584 1108 22-23 0.7112195280590754 1108 24-25 0.7956979937289006 1108 26-27 0.7795626446471218 1108 28-29 0.8020402073441986 1108 30-31 0.7598038921153076 1108 32-33 0.9317128805993917 1108 34-35 1.1573187802260918 1108 36-37 1.234691903235536 1108 38-39 1.235010107696688 1108 40-41 1.4309449744906289 1108 42-43 1.5823767003411007 1108 44-45 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1.6059532550423405 2311 74-75 1.8627054970059724 2311 76-77 2.0362674973739026 2311 78-79 2.1241818502525227 2311 80-81 2.216783271377711 2311 82-83 2.3822013211062654 2311 84-85 2.615329300492345 2311 86-87 2.8348479263375665 2311 88-89 2.717406998470537 2311 90-91 2.801062059199136 2311 92-93 2.944965371554847 2311 94-95 2.893913103058903 2311 96-97 2.5070256450155526 2311 98-99 2.9387987677286187 2311 100-101 2.973222340754303 2312 1 -0.3402843570450713 2312 2 -0.3569678091876298 2312 3 -0.35152568648038596 2312 4 -0.32691766481090667 2312 5 -0.3136477924933203 2312 6 -0.1677214788905772 2312 7 -0.24964274940439424 2312 8 -0.3407967552119473 2312 9 -0.2989019602774974 2312 10-11 -0.3601269818161228 2312 12-13 -0.34822105520333935 2312 14-15 -0.21680132037870692 2312 16-17 -0.21192050306555643 2312 18-19 -0.08162400075993759 2312 20-21 -0.16876289153398716 2312 22-23 -0.31944591591697247 2312 24-25 -0.3255667928101005 2312 26-27 -0.10990478383077118 2312 28-29 -0.08052790939080268 2312 30-31 -0.08543591330346345 2312 32-33 -0.03198785991156683 2312 34-35 -0.04532301558953833 2312 36-37 0.08663006054023725 2312 38-39 0.16332324107078477 2312 40-41 0.18153769517945761 2312 42-43 0.1630037408813365 2312 44-45 0.21752842554440122 2312 46-47 0.2704948875295372 2312 48-49 0.4493686797527445 2312 50-51 0.6137337753578969 2312 52-53 0.7036147063463396 2312 54-55 0.8071174611478433 2312 56-57 0.827994699019154 2312 58-59 0.8816842076418041 2312 60-61 1.0540689573843842 2312 62-63 1.1734634021307748 2312 64-65 1.3102884674993263 2312 66-67 1.3129160270980407 2312 68-69 1.6058193236481308 2312 70-71 1.7374164103450198 2312 72-73 1.606026737674469 2312 74-75 1.5720399244294363 2312 76-77 1.630959530346761 2312 78-79 1.9322271388245689 2312 80-81 2.215012693158741 2312 82-83 2.3349575292805547 2312 84-85 2.6352993312137656 2312 86-87 2.911992569938487 2312 88-89 2.8747663498730702 2312 90-91 2.860503621668446 2312 92-93 3.0888707525197 2312 94-95 3.196216773187402 2312 96-97 3.3661894278986857 2312 98-99 3.186833886470593 2312 100-101 3.021364199772089 2313 1 -0.6064232741113429 2313 2 -0.7443221284513015 2313 3 -0.5681111967911114 2313 4 -0.680196606644003 2313 5 -0.4927785604404775 2313 6 -0.6914176527677256 2313 7 -0.7173070052579718 2313 8 -0.6325644213340951 2313 9 -0.6910456572527863 2313 10-11 -0.6218925950145575 2313 12-13 -0.6847195413093274 2313 14-15 -0.6781986011879297 2313 16-17 -0.5938028063142298 2313 18-19 -0.6167726346325253 2313 20-21 -0.6970855540552918 2313 22-23 -0.827284214690934 2313 24-25 -0.6454743343955229 2313 26-27 -0.47185575024186477 2313 28-29 -0.4966575361538972 2313 30-31 -0.39970470743583775 2313 32-33 -0.3896882724723838 2313 34-35 -0.2969413397450893 2313 36-37 -0.22895611779406266 2313 38-39 -0.24984947568282934 2313 40-41 -0.2737946781101357 2313 42-43 -0.08670451340894658 2313 44-45 -0.1697810203198884 2313 46-47 -0.14094299890316364 2313 48-49 0.06927163793896796 2313 50-51 0.2814263549718099 2313 52-53 0.315682865060392 2313 54-55 0.22416273854503999 2313 56-57 0.5706093882921373 2313 58-59 0.35572988629227353 2313 60-61 0.39224964705696763 2313 62-63 0.6495574073739618 2313 64-65 0.5621002824595429 2313 66-67 0.6854217110619594 2313 68-69 1.1823102869414193 2313 70-71 1.3052509929977063 2313 72-73 1.2576109333455427 2313 74-75 1.2486115363368349 2313 76-77 1.4723782406056678 2313 78-79 1.805805187251611 2313 80-81 2.175934274766302 2313 82-83 1.9933012095689726 2313 84-85 2.0599982677553683 2313 86-87 1.9995768001908978 2313 88-89 2.1612936400357228 2313 90-91 2.4047377466399062 2313 92-93 2.2632835696799454 2313 94-95 2.166619198381934 2313 96-97 2.277973882435756 2313 98-99 2.0125337331051174 2313 100-101 1.9210290627202191 2314 1 -0.4303875065434468 2314 2 -0.5894117947526034 2314 3 -0.7210396235966172 2314 4 -0.5910602727626255 2314 5 -0.6286214845666507 2314 6 -0.5339098126751693 2314 7 -0.7360509158656896 2314 8 -0.4232567852361768 2314 9 -0.6548432686886656 2314 10-11 -0.51961173701919 2314 12-13 -0.6662905664585139 2314 14-15 -0.6437026696631492 2314 16-17 -0.546609540420917 2314 18-19 -0.3251154826412943 2314 20-21 -0.43232638951990765 2314 22-23 -0.611279198554044 2314 24-25 -0.5614216915992714 2314 26-27 -0.3696857382153098 2314 28-29 -0.4375918582523113 2314 30-31 -0.30400086883518185 2314 32-33 -0.2490699327451935 2314 34-35 -0.2082179427776083 2314 36-37 -0.17435567707429556 2314 38-39 -0.03565985961900964 2314 40-41 0.08931150186185732 2314 42-43 0.3357033084154324 2314 44-45 0.15101292225713792 2314 46-47 0.3185254352096152 2314 48-49 0.12178408313443612 2314 50-51 -0.07485256964151077 2314 52-53 -0.028561422328476027 2314 54-55 0.13718433305240652 2314 56-57 0.09812773258637719 2314 58-59 0.6436720332597581 2314 60-61 0.544009195985236 2314 62-63 0.7019100060221142 2314 64-65 0.769349894260781 2314 66-67 1.0565630344391366 2314 68-69 1.40183049991791 2314 70-71 1.2696109217518199 2314 72-73 1.3427677542260668 2314 74-75 1.2878548151701104 2314 76-77 1.3765621288348733 2314 78-79 1.8207337130097407 2314 80-81 1.9114291520650433 2314 82-83 2.2289254864536403 2314 84-85 2.266903924039621 2314 86-87 2.1689370714733265 2314 88-89 2.3363822216640244 2314 90-91 2.3178444974924197 2314 92-93 2.3680900982696436 2314 94-95 2.387095636239689 2314 96-97 2.5050944181500405 2314 98-99 2.6958724898034507 2314 100-101 2.8395596543046793 2315 1 -0.7895829963335679 2315 2 -0.674120582920807 2315 3 -0.7078289808345559 2315 4 -0.7471543656247874 2315 5 -0.5281534560127454 2315 6 -0.5489129535196042 2315 7 -0.7727320261435153 2315 8 -0.765396501534596 2315 9 -0.9603713151475262 2315 10-11 -0.7328601179886576 2315 12-13 -0.6919837956952932 2315 14-15 -0.8836046945463281 2315 16-17 -0.826209996163854 2315 18-19 -0.7068546104052871 2315 20-21 -0.7041083193017386 2315 22-23 -0.8169393420927662 2315 24-25 -0.7194091714882518 2315 26-27 -0.7849921067581533 2315 28-29 -0.9060044711998501 2315 30-31 -0.7306198860072683 2315 32-33 -0.7556487465743089 2315 34-35 -0.7673426033206994 2315 36-37 -0.6573806948617289 2315 38-39 -0.6863508854572657 2315 40-41 -0.6193166862555017 2315 42-43 -0.47947028199708086 2315 44-45 -0.4445108135529665 2315 46-47 -0.5481132683267234 2315 48-49 -0.5255944334896014 2315 50-51 -0.27029148128549707 2315 52-53 -0.12419612930050761 2315 54-55 0.05824664623091991 2315 56-57 0.25843572496715694 2315 58-59 0.5470578154902697 2315 60-61 0.3616229501062591 2315 62-63 0.47566564672233014 2315 64-65 0.5464034883910394 2315 66-67 0.4870057492825097 2315 68-69 0.7564964032739177 2315 70-71 0.7562874382775746 2315 72-73 0.9284514460857238 2315 74-75 1.2392848341647387 2315 76-77 1.1199574751503647 2315 78-79 1.4627470210026559 2315 80-81 1.5886392141982135 2315 82-83 1.6501320888505084 2315 84-85 2.067781397454617 2315 86-87 2.2410724664398565 2315 88-89 2.31728583406246 2315 90-91 2.1939079377426616 2315 92-93 2.2056623029715077 2315 94-95 2.314811945875668 2315 96-97 2.43867837804979 2315 98-99 2.210407976004033 2315 100-101 2.067965414015122 2316 1 -1.3393356599560917 2316 2 -1.2363652356485169 2316 3 -0.9894069268411236 2316 4 -0.8863436630693116 2316 5 -1.016757958135635 2316 6 -0.9491672994300444 2316 7 -0.9903079293719088 2316 8 -0.9557053215297913 2316 9 -1.1323151587748583 2316 10-11 -0.9420946769889014 2316 12-13 -1.1121952833184636 2316 14-15 -1.5974855725403998 2316 16-17 -1.4117413187625871 2316 18-19 -1.231801037546198 2316 20-21 -0.9958325642748989 2316 22-23 -1.3254308905987386 2316 24-25 -1.3265123317862546 2316 26-27 -1.2223594263127495 2316 28-29 -1.2893358018094787 2316 30-31 -1.0673303121868756 2316 32-33 -0.9756529522940909 2316 34-35 -1.0365637440721258 2316 36-37 -0.8631505418536349 2316 38-39 -0.845660045915487 2316 40-41 -0.7885167984080255 2316 42-43 -0.46046443404387105 2316 44-45 -0.668610589247919 2316 46-47 -0.4071064991206015 2316 48-49 -0.3366674928503315 2316 50-51 -0.2698959433539123 2316 52-53 -0.2279772716541082 2316 54-55 -0.33964698911314883 2316 56-57 -0.030795128994231646 2316 58-59 0.10821599060842857 2316 60-61 0.1579910106686242 2316 62-63 0.22036593911999347 2316 64-65 0.505853340189482 2316 66-67 0.7654093782178641 2316 68-69 0.8585977611205884 2316 70-71 0.5617028031738336 2316 72-73 0.9978032645588506 2316 74-75 1.3121188884786044 2316 76-77 1.2602507740368516 2316 78-79 1.038685297058091 2316 80-81 1.2376443611981323 2316 82-83 1.6322427593624091 2316 84-85 1.698747010688617 2316 86-87 1.9117095328501748 2316 88-89 2.011610115084647 2316 90-91 2.3494820184924805 2316 92-93 2.4096171816064533 2316 94-95 2.4008965208976996 2316 96-97 2.356556692557522 2316 98-99 2.3630455631225136 2316 100-101 2.0332732326484635 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13710.0 3 5505.0 4 5855.0 5 6616.0 6 7311.0 7 9071.0 8 11231.0 9 13899.0 10 16678.0 11 19839.0 12 23165.0 13 26299.0 14 29063.0 15 32178.0 16 35748.0 17 39454.0 18 39879.0 19 40236.0 20 43112.0 21 46283.0 22 50859.0 23 55229.0 24 60574.0 25 66588.0 26 72920.0 27 79593.0 28 86964.0 29 95648.0 30 105373.0 31 117056.0 32 131436.0 33 148354.0 34 170113.0 35 196201.0 36 219934.0 37 213034.0 38 109047.0 39 7526.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.2395754362465 18.220949732898617 14.49801837245106 24.04145645840382 2 15.61291812592753 19.42627636615584 40.33174645727574 24.62905905064089 3 19.563126466763162 23.22471413548556 28.94832953758525 28.26382986016603 4 13.156252741804172 16.088611030061394 36.075756431890014 34.67937979624442 5 14.327157558776655 36.60843249332188 33.91881771227652 15.145592235624946 6 33.501497849137614 35.004203772784045 17.091519806708078 14.402778571370266 7 29.540996837373424 31.604558654257715 20.1966206288449 18.65782387952396 8 26.458947255030452 35.143468961234284 19.401870592718872 18.99571319101639 9 26.267096441948713 16.91948405746714 19.681357456572577 37.13206204401157 10-11 25.317152189391606 26.050526461821573 27.110713054335427 21.52160829445139 12-13 26.207980525173173 23.243402488255143 27.339142839130904 23.209474147440776 14-15 22.91173219643139 24.98838168145121 25.37761065351869 26.722275468598717 16-17 22.860470306290264 27.99147382683521 26.199092548839452 22.94896331803507 18-19 22.841679947318042 26.61801582580355 28.19802573223106 22.342278494647346 20-21 23.15415866196804 26.014672061711124 28.40184049580437 22.42932878051647 22-23 22.960501646189773 25.871456428096657 28.274048876065923 22.893993049647648 24-25 22.875283511491972 26.056978795431984 28.288646582710875 22.779091110365172 26-27 22.499149148373014 26.38470491159713 28.139499674758394 22.976646265271462 28-29 22.619707545343203 26.712505822386156 28.104635037635617 22.56315159463502 30-31 22.688710955870274 26.283683356303218 28.029673487583512 22.997932200242996 32-33 22.457958042505894 26.308407248998883 28.040171929919055 23.19346277857617 34-35 23.139213607320226 26.400558838272502 27.765542536442716 22.694685017964552 36-37 23.275344649532016 25.93975574735507 27.799090346385686 22.98580925672723 38-39 22.74500788322907 26.264789596626315 27.85471521949729 23.135487300647327 40-41 23.06862853292693 26.20872697438274 27.83191556938788 22.890728923302444 42-43 22.86293389863597 26.170643796715442 27.724022485630297 23.242399819018296 44-45 22.824117993445668 26.486049662707156 27.79104140911369 22.898790934733483 46-47 23.164408467192413 26.687264921750902 27.22671971869858 22.921606892358103 48-49 23.02442817413483 26.565634272142074 27.29881810211784 23.11111945160526 50-51 23.17446124237026 26.831739227804867 27.122916570634164 22.87088295919071 52-53 23.266969601232244 26.582701891053283 27.178835345157964 22.971493162556506 54-55 22.865724501985895 26.734658787396565 27.31995423860906 23.079662472008476 56-57 22.970483825405548 26.887671696677785 27.205626052309807 22.93621842560686 58-59 22.96920870012346 26.489965592997116 27.244924870191017 23.295900836688404 60-61 22.786034683627747 26.707604910794412 27.374688751317606 23.131671654260234 62-63 22.751019186070703 26.751339810546014 27.540765403232186 22.956875600151093 64-65 22.644919517995223 26.631133034305282 27.40581707535715 23.318130372342345 66-67 22.57696607225277 26.731289198918954 27.25347575390518 23.438268974923094 68-69 22.886111279383094 26.440586630801278 27.482853085098508 23.190449004717124 70-71 22.75816082087969 26.49302960480152 27.252629706628202 23.49617986769059 72-73 22.824796341583564 26.53371814780735 27.220293367661753 23.42119214294733 74-75 22.765783981947468 26.86945916854816 27.02829387214612 23.336462977358252 76-77 22.94541678488478 26.529490821413106 27.10723829365318 23.41785410004893 78-79 22.980447874255493 26.581463927223393 27.099790450293238 23.338297748227884 80-81 23.027047643911782 26.74855134687517 26.900443213409396 23.323957795803647 82-83 23.00524968378901 26.686188356704356 26.932385206894082 23.37617675261255 84-85 23.028235142468805 26.608597078341116 26.96007805884109 23.40308972034898 86-87 23.115528182385912 26.645704409742578 26.793651687157794 23.44511572071372 88-89 23.08060433669348 26.639193108028934 26.73005086728974 23.550151687987846 90-91 23.01267735309124 26.59626707533846 26.75767937584524 23.633376195725063 92-93 23.056162677678838 26.242219459434104 26.944500255484243 23.757117607402822 94-95 22.908501245302197 26.177906550010064 27.032268106453195 23.881324098234547 96-97 23.1401580219292 25.926868807283864 26.965416358388662 23.967556812398275 98-99 22.988264489478876 26.086538808694637 26.670387239414573 24.254809462411913 100-101 23.43780816413697 25.50809949779071 26.96613568348279 24.087956654589522 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 305.0 1 223.0 2 208.0 3 295.5 4 267.0 5 231.5 6 403.0 7 863.0 8 1334.0 9 1592.5 10 1514.0 11 1368.5 12 1425.5 13 1524.5 14 1667.0 15 1845.5 16 2006.0 17 2176.0 18 2367.0 19 2645.0 20 3045.5 21 3603.0 22 4437.5 23 5500.5 24 6860.5 25 8700.5 26 11154.0 27 14099.0 28 16932.5 29 20082.5 30 23743.0 31 28609.0 32 35166.5 33 39134.0 34 41339.0 35 44957.0 36 50105.5 37 55672.5 38 59947.5 39 63463.5 40 67265.0 41 72206.5 42 76618.5 43 80378.0 44 83527.0 45 87461.5 46 90341.0 47 92330.5 48 93689.0 49 93500.0 50 93282.0 51 91457.5 52 90070.0 53 90154.5 54 89945.5 55 85326.5 56 77555.5 57 70970.5 58 65444.0 59 57821.5 60 48281.0 61 40984.0 62 34598.0 63 27275.0 64 20967.0 65 16380.0 66 12520.5 67 9674.0 68 7470.5 69 5648.5 70 4488.0 71 3416.0 72 2608.0 73 2031.5 74 1537.0 75 1195.0 76 865.5 77 580.0 78 361.5 79 234.0 80 159.0 81 98.0 82 65.0 83 37.5 84 24.5 85 23.0 86 16.5 87 9.5 88 7.5 89 5.0 90 4.5 91 2.5 92 1.5 93 2.5 94 1.5 95 0.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.30094865313444674 2 0.03483466383529649 3 0.04209528463469084 4 0.044705844922113526 5 0.057758646359226964 6 0.05714679629186228 7 0.03140830345805421 8 0.03291753362422045 9 0.04070842448199754 10-11 0.03206094352990988 12-13 0.03375372871628553 14-15 0.02896090318859544 16-17 0.022026602425128924 18-19 0.026391132905663733 20-21 0.038220234208047786 22-23 0.03140830345805421 24-25 0.052353970764172184 26-27 0.04466505491762255 28-29 0.03713929908903683 30-31 0.037057719080054875 32-33 0.03324385366014829 34-35 0.03191817851419146 36-37 0.03091882340416246 38-39 0.02004828720731642 40-41 0.019558807153424666 42-43 0.020578557265699154 44-45 0.009157356008224897 46-47 0.016417976807619247 48-49 0.014439661589806741 50-51 0.006281660691610842 52-53 0.004752035523199112 54-55 0.006913905761221024 56-57 0.005241515577090865 58-59 0.007770495855531593 60-61 0.006730350741011616 62-63 0.004690850516462642 64-65 0.007933655873495512 66-67 0.0025493752806862186 68-69 0.007852075864513553 70-71 0.014562031603279681 72-73 0.01454163660103419 74-75 0.011849496304629543 76-77 0.010217896124990362 78-79 0.005465860601791252 80-81 0.006771140745502596 82-83 0.011849496304629543 84-85 0.003956630435625011 86-87 0.0013052801437113438 88-89 0.020272632232016807 90-91 0.029144458208804846 92-93 0.014419266587561251 94-95 0.02322990755761282 96-97 0.018987747090550955 98-99 0.014092946551633415 100-101 0.10613559168552865 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2451581.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.402879135395786 #Duplication Level Percentage of deduplicated Percentage of total 1 81.17178685769456 49.84181417627126 2 11.156972271943834 13.701404398622586 3 3.310578274297625 6.098371129348926 4 1.3842178601005446 3.399798478432397 5 0.7328083669748688 2.249827179338231 6 0.4643055263052707 1.7105817668171324 7 0.31396986154293094 1.3495057422334276 8 0.24275006868960405 1.1924442502285426 9 0.1641123971064686 0.9069276315733711 >10 0.9606083123566317 10.780362689405194 >50 0.06193127930162406 2.6188851456538877 >100 0.0319403894581851 3.430814884373348 >500 0.002612047248086554 1.1343489050794864 >1k 0.0013395114092722299 1.3611117612788908 >5k 6.697557046361149E-5 0.22380186134331584 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5442 0.22197920443991043 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4503 0.18367739022288068 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2913 0.11882128308222327 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.0790004490979495E-5 0.0 5 0.0 0.0 0.0 4.0790004490979495E-5 0.0 6 0.0 0.0 0.0 8.158000898195899E-5 0.0 7 0.0 0.0 0.0 8.158000898195899E-5 0.0 8 0.0 0.0 0.0 8.158000898195899E-5 0.0 9 0.0 0.0 0.0 1.2237001347293849E-4 0.0 10-11 0.0 0.0 0.0 1.2237001347293849E-4 0.0 12-13 0.0 0.0 0.0 1.8355502020940773E-4 4.0790004490979495E-5 14-15 0.0 0.0 0.0 2.0395002245489748E-4 4.0790004490979495E-5 16-17 0.0 0.0 0.0 2.2434502470038722E-4 4.0790004490979495E-5 18-19 0.0 0.0 0.0 3.467150381733257E-4 4.0790004490979495E-5 20-21 0.0 0.0 0.0 4.0790004490979495E-4 4.0790004490979495E-5 22-23 0.0 0.0 0.0 5.098750561372436E-4 4.0790004490979495E-5 24-25 0.0 0.0 0.0 7.342200808376309E-4 4.0790004490979495E-5 26-27 0.0 0.0 0.0 0.001203305132483895 4.0790004490979495E-5 28-29 0.0 0.0 0.0 0.003038855334577972 4.0790004490979495E-5 30-31 0.0 0.0 0.0 0.009402096035170773 4.0790004490979495E-5 32-33 2.0395002245489748E-5 0.0 0.0 0.02416807766090535 4.0790004490979495E-5 34-35 4.0790004490979495E-5 0.0 0.0 0.04588875505235193 4.0790004490979495E-5 36-37 4.0790004490979495E-5 0.0 0.0 0.0839254342401903 4.0790004490979495E-5 38-39 4.0790004490979495E-5 0.0 0.0 0.1404399854624424 4.0790004490979495E-5 40-41 4.0790004490979495E-5 0.0 0.0 0.19946312196088972 4.0790004490979495E-5 42-43 6.118500673646924E-5 0.0 0.0 0.2614231387826876 4.0790004490979495E-5 44-45 1.6316001796391798E-4 0.0 0.0 0.34184063263665365 4.0790004490979495E-5 46-47 2.0395002245489748E-4 0.0 0.0 0.43947150838581306 6.118500673646924E-5 48-49 2.0395002245489748E-4 0.0 0.0 0.540243214480778 8.158000898195899E-5 50-51 2.0395002245489748E-4 0.0 0.0 0.6489689714514838 8.158000898195899E-5 52-53 2.0395002245489748E-4 0.0 0.0 0.7532894079371638 8.158000898195899E-5 54-55 2.0395002245489748E-4 0.0 0.0 0.8730692561249251 8.158000898195899E-5 56-57 2.0395002245489748E-4 0.0 0.0 1.0138763516277862 8.158000898195899E-5 58-59 2.0395002245489748E-4 0.0 0.0 1.1631473730625257 8.158000898195899E-5 60-61 2.0395002245489748E-4 0.0 0.0 1.328408076257729 8.158000898195899E-5 62-63 2.0395002245489748E-4 0.0 0.0 1.4944845795427522 8.158000898195899E-5 64-65 2.0395002245489748E-4 0.0 0.0 1.6636815181713351 8.158000898195899E-5 66-67 2.4474002694587697E-4 0.0 0.0 1.8647354503073732 8.158000898195899E-5 68-69 2.4474002694587697E-4 0.0 0.0 2.0953825307016167 8.158000898195899E-5 70-71 2.4474002694587697E-4 0.0 0.0 2.340510062690158 8.158000898195899E-5 72-73 2.651350291913667E-4 0.0 0.0 2.590512000215371 8.158000898195899E-5 74-75 2.8553003143685647E-4 0.0 0.0 2.8469383634479137 1.2237001347293849E-4 76-77 2.8553003143685647E-4 0.0 0.0 3.1257788341482495 1.2237001347293849E-4 78-79 3.059250336823462E-4 0.0 0.0 3.4385565885850804 1.2237001347293849E-4 80-81 3.6711004041881546E-4 0.0 0.0 3.7739931905166504 1.2237001347293849E-4 82-83 3.6711004041881546E-4 0.0 0.0 4.115140393076958 1.2237001347293849E-4 84-85 3.875050426643052E-4 0.0 0.0 4.459183685956123 1.2237001347293849E-4 86-87 4.0790004490979495E-4 0.0 0.0 4.818135725476743 1.2237001347293849E-4 88-89 4.0790004490979495E-4 0.0 0.0 5.202030852743597 1.2237001347293849E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3070 0.0 52.635086 1 GTATCAA 5765 0.0 45.80622 1 ATCAACG 7600 0.0 34.205845 3 TATCAAC 7660 0.0 34.062 2 TCAACGC 7720 0.0 33.79727 4 CAACGCA 7965 0.0 32.81735 5 AACGCAG 8285 0.0 31.605238 6 ACGCAGA 9455 0.0 27.336308 7 CGCAGAG 9505 0.0 26.942577 8 GTACATG 7280 0.0 26.32138 1 TACATGG 7470 0.0 26.021317 2 ACATGGG 7700 0.0 24.318237 3 GCAGAGT 10540 0.0 23.214073 9 TGGTATC 1485 0.0 22.08255 2 AGAGTAC 9545 0.0 20.652925 10-11 GAGTACT 6325 0.0 20.464546 12-13 CATGGGG 5305 0.0 20.246485 4 AGTACTT 6685 0.0 19.611185 12-13 GTGGTAT 1850 0.0 19.581947 1 CAGAGTA 10140 0.0 19.58158 10-11 >>END_MODULE