FastQCFastQC Report
Thu 26 May 2016
SRR938481_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938481_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2451581
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54650.22291737454320296No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47200.1925288211974232No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31700.129304314236405No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33200.051.577221
GTATCAA59300.042.512311
ATCAACG76750.031.8420373
TATCAAC79350.031.1709422
TCAACGC79000.030.9351444
CAACGCA80350.030.415395
AACGCAG84250.029.0888276
TGGTATC14700.028.1511062
GTGGTAT19200.025.764731
ACGCAGA97200.025.1155837
CGCAGAG97150.025.0307358
GTACATG72050.022.842081
TACATGG71100.022.7458842
GCAGAGT108750.021.8367089
ACATGGG71500.021.6119253
GTATACG1856.502125E-620.5605344
AGAGTAC98700.020.09026710-11
GGACCGA7600.019.9978286
TAACGCA1707.899273E-519.5778624
CAGAGTA104100.018.95687310-11
Produced by FastQC (version 0.11.5)