FastQCFastQC Report
Thu 26 May 2016
SRR938480_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938480_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2455490
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51310.2089603297101597No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42120.17153399117895002No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27370.11146451421101289No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34050.055.6285741
GTATCAA60450.044.1993871
TATCAAC77650.034.0336072
ATCAACG78050.033.859183
TCAACGC79500.033.2416274
CAACGCA80700.032.5117345
AACGCAG83550.031.3950376
ACGCAGA95550.027.29637
CGCAGAG95900.027.0480658
GTACATG71850.024.1712511
GCAGAGT105000.023.8427839
TACATGG71850.023.219612
GAGTACT64950.022.3781812-13
TGGTATC15050.021.7915022
ACATGGG74450.021.451083
CAGAGTA101400.021.12661710-11
GTACTTT68850.020.83673914-15
AGAGTAC96550.020.76115810-11
GTGGTAT18100.020.5608251
TAAGGTG13700.019.7755645
Produced by FastQC (version 0.11.5)