##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938480_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2455490 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.615385523866927 31.0 31.0 34.0 27.0 34.0 2 30.81161601146818 31.0 31.0 34.0 27.0 34.0 3 30.855619041413323 31.0 31.0 34.0 27.0 34.0 4 34.513729642556065 37.0 35.0 37.0 30.0 37.0 5 34.47995512097382 37.0 35.0 37.0 30.0 37.0 6 34.43133305368786 37.0 35.0 37.0 30.0 37.0 7 34.3230426513649 37.0 35.0 37.0 30.0 37.0 8 34.26417212043217 37.0 35.0 37.0 30.0 37.0 9 35.67769528688775 38.0 35.0 39.0 30.0 39.0 10-11 35.473373135300896 38.0 35.0 39.0 30.0 39.0 12-13 35.2544557298136 38.0 35.0 39.0 29.0 39.0 14-15 36.331726661481014 39.0 36.0 41.0 29.0 41.0 16-17 36.23428114144224 39.0 36.0 41.0 27.5 41.0 18-19 36.194803684804256 39.0 35.5 41.0 27.0 41.0 20-21 36.089714272914975 39.0 35.0 41.0 27.0 41.0 22-23 35.897083474174195 39.0 35.0 40.0 27.0 41.0 24-25 35.725827838842754 39.0 34.5 40.0 27.0 41.0 26-27 35.46684287046577 38.0 34.0 40.0 26.5 41.0 28-29 35.183439761513995 38.0 34.0 40.0 25.5 41.0 30-31 34.87559733495148 38.0 34.0 40.0 25.0 41.0 32-33 34.54164097593556 38.0 33.5 40.0 24.0 41.0 34-35 34.18587491702267 38.0 33.0 40.0 24.0 41.0 36-37 33.90617758573645 38.0 33.0 40.0 22.0 41.0 38-39 33.59626245678052 38.0 32.5 40.0 20.0 41.0 40-41 33.261959120175604 37.5 31.5 40.0 18.0 41.0 42-43 32.78835344472997 37.0 31.0 40.0 15.5 41.0 44-45 32.26000533498406 37.0 30.0 40.0 11.5 41.0 46-47 31.732461138102785 36.5 30.0 39.0 8.5 41.0 48-49 31.19005636349568 36.0 29.5 39.0 7.5 40.0 50-51 30.469519525634396 35.5 28.5 38.5 3.5 40.0 52-53 30.45500063123857 35.0 28.0 38.5 2.0 40.0 54-55 30.347268976864086 35.0 28.0 39.0 2.0 40.0 56-57 29.82764845305825 35.0 27.0 39.0 2.0 40.0 58-59 29.293381158139518 34.5 26.0 38.5 2.0 40.0 60-61 28.67241446717356 34.0 25.5 38.0 2.0 40.0 62-63 27.87477957556333 34.0 23.5 38.0 2.0 40.0 64-65 27.137842141487035 33.0 21.5 37.0 2.0 39.5 66-67 26.364929199467316 32.5 19.0 36.5 2.0 39.0 68-69 25.438627524445224 31.5 13.5 36.0 2.0 39.0 70-71 24.530217797669714 31.0 7.5 35.5 2.0 38.5 72-73 23.57267856924687 30.0 2.0 35.0 2.0 38.0 74-75 22.58344037239003 29.0 2.0 34.0 2.0 37.0 76-77 21.581360746734866 28.5 2.0 34.0 2.0 36.0 78-79 20.552622898077367 27.0 2.0 34.0 2.0 36.0 80-81 19.56345780271962 26.0 2.0 33.0 2.0 35.0 82-83 18.547066776895853 24.5 2.0 32.5 2.0 35.0 84-85 17.56464595661151 21.0 2.0 32.0 2.0 34.5 86-87 16.578018643936648 17.0 2.0 31.5 2.0 34.0 88-89 15.505622503044199 4.5 2.0 31.0 2.0 34.0 90-91 14.395478295574407 2.0 2.0 30.0 2.0 34.0 92-93 13.302261259463489 2.0 2.0 29.5 2.0 34.0 94-95 12.146797991439591 2.0 2.0 29.0 2.0 34.0 96-97 10.877301475469256 2.0 2.0 26.0 2.0 33.0 98-99 9.407084125775304 2.0 2.0 13.0 2.0 32.0 100-101 6.729286618964036 2.0 2.0 2.0 2.0 27.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6242749445210727 1101 2 0.6345255024404004 1101 3 0.647775283941872 1101 4 0.5555811023970705 1101 5 0.5778039010781839 1101 6 0.6996557203296447 1101 7 0.534441455394699 1101 8 0.5593658504187076 1101 9 0.5621154229635863 1101 10-11 0.781313272216849 1101 12-13 0.7750994244818159 1101 14-15 0.9588773049909918 1101 16-17 0.9510162240269651 1101 18-19 0.9987235851999188 1101 20-21 1.1462493103343192 1101 22-23 1.1659883381905303 1101 24-25 1.1171581680397438 1101 26-27 1.2303868727854592 1101 28-29 1.3383298272589101 1101 30-31 1.4066418950534754 1101 32-33 1.4231482016595365 1101 34-35 1.3290968872518363 1101 36-37 1.3880298900660648 1101 38-39 1.4515537942030576 1101 40-41 1.4780954278587615 1101 42-43 1.564130741569194 1101 44-45 1.8403529255296576 1101 46-47 1.826781997768947 1101 48-49 1.6713992505612616 1101 50-51 1.8043093581163276 1101 52-53 1.885781237028258 1101 54-55 1.9781396290249802 1101 56-57 2.35444327962767 1101 58-59 2.335865662392031 1101 60-61 2.3980076938659742 1101 62-63 2.2989798305763074 1101 64-65 2.3654300635925907 1101 66-67 2.4482898768108896 1101 68-69 2.685198734673339 1101 70-71 3.019493222224426 1101 72-73 3.1315992693291825 1101 74-75 3.264741483039284 1101 76-77 3.5885329493202356 1101 78-79 3.8435862824717013 1101 80-81 4.295650380692727 1101 82-83 3.958954191399858 1101 84-85 3.9472669162117135 1101 86-87 4.009891350127294 1101 88-89 4.415832195589497 1101 90-91 4.340747081427013 1101 92-93 4.264500496978785 1101 94-95 4.291597555112036 1101 96-97 4.293845749051604 1101 98-99 4.030667929091647 1101 100-101 2.8450499669939573 1102 1 0.8955316581396531 1102 2 0.7682892020112995 1102 3 0.7429859992086669 1102 4 0.683486074500621 1102 5 0.7040815015101316 1102 6 0.6840100288861279 1102 7 0.8268341085409858 1102 8 0.8268402036852933 1102 9 0.9556949775676884 1102 10-11 1.0175393423700143 1102 12-13 1.2017672909956545 1102 14-15 1.3538810466196907 1102 16-17 1.3759335766588876 1102 18-19 1.1595643623219942 1102 20-21 1.185859896775412 1102 22-23 1.0686808899813016 1102 24-25 1.2937057340865934 1102 26-27 1.4027457091862985 1102 28-29 1.3111859687398422 1102 30-31 1.252517104632048 1102 32-33 1.3773587180042952 1102 34-35 1.5727072274085074 1102 36-37 1.6649454618721862 1102 38-39 1.705390147678031 1102 40-41 1.912628766244083 1102 42-43 2.0485783540311857 1102 44-45 2.115528876803225 1102 46-47 1.9871766211905992 1102 48-49 2.063726543611306 1102 50-51 2.238001540480468 1102 52-53 2.238707285394728 1102 54-55 2.2537063200043406 1102 56-57 2.750044260976363 1102 58-59 3.0110117753607994 1102 60-61 2.851693434945272 1102 62-63 2.7092631523847928 1102 64-65 2.9323616438860505 1102 66-67 3.2107949682676633 1102 68-69 3.258087556202387 1102 70-71 3.1335570098893655 1102 72-73 3.313903733707928 1102 74-75 3.17918322256509 1102 76-77 3.4295037835613478 1102 78-79 3.9040993118647975 1102 80-81 4.050773570265424 1102 82-83 4.018734051017738 1102 84-85 4.118077002505988 1102 86-87 4.121876876181343 1102 88-89 4.204402703977374 1102 90-91 4.184885985840241 1102 92-93 4.320097310437278 1102 94-95 4.43617124625243 1102 96-97 4.199783098083989 1102 98-99 3.7730530042557557 1102 100-101 2.432718695503273 1103 1 0.7014752773359945 1103 2 0.5663956677224924 1103 3 0.6090335236018269 1103 4 0.4731762009409124 1103 5 0.6129645802124983 1103 6 0.6094184144332431 1103 7 0.6498142210239948 1103 8 0.6214609240567768 1103 9 0.741455438423614 1103 10-11 0.8617831795858137 1103 12-13 0.8537966865071525 1103 14-15 0.9947099787825522 1103 16-17 0.9997123606803271 1103 18-19 1.110153674694665 1103 20-21 1.1551293253884722 1103 22-23 1.153465807690182 1103 24-25 1.1811504158872381 1103 26-27 1.2120122235417696 1103 28-29 1.3020355664714884 1103 30-31 1.1935637813244924 1103 32-33 1.5405275424129385 1103 34-35 1.44339575283999 1103 36-37 1.5208241517759333 1103 38-39 1.659345519213801 1103 40-41 1.8623272138978777 1103 42-43 1.8976945916696408 1103 44-45 1.9428766182994153 1103 46-47 2.258922252324396 1103 48-49 2.1136527249166157 1103 50-51 2.215472771351074 1103 52-53 2.315117378256904 1103 54-55 2.382109609266408 1103 56-57 2.5937543674933927 1103 58-59 2.637859969116775 1103 60-61 2.549913446730603 1103 62-63 2.837062787094517 1103 64-65 3.1805364296926975 1103 66-67 3.1441979505245996 1103 68-69 3.0023842716141367 1103 70-71 3.246365813513311 1103 72-73 3.4664621861088456 1103 74-75 3.3269212363060916 1103 76-77 3.5328846889423673 1103 78-79 3.5575886771156675 1103 80-81 3.6351107187101945 1103 82-83 3.9905843388235844 1103 84-85 4.099984228306884 1103 86-87 4.090323714349321 1103 88-89 4.546741551489303 1103 90-91 4.38920486060689 1103 92-93 4.579064655172877 1103 94-95 4.254945296896361 1103 96-97 3.97634935116435 1103 98-99 3.646460495364062 1103 100-101 2.5899840404899708 1104 1 0.7776294825130492 1104 2 0.6759460372729542 1104 3 0.7054475230368098 1104 4 0.6962233056958453 1104 5 0.6343497756551102 1104 6 0.7898721461390465 1104 7 0.7348733496125277 1104 8 0.7031582575197888 1104 9 0.7591042195992088 1104 10-11 0.8856085453154776 1104 12-13 0.871681148406374 1104 14-15 1.042073056258218 1104 16-17 0.9847736505331355 1104 18-19 1.1221008348706007 1104 20-21 1.0333196988086328 1104 22-23 1.2717860216347603 1104 24-25 1.3111895707917398 1104 26-27 1.4861500720649303 1104 28-29 1.3850067593915938 1104 30-31 1.4102534242887828 1104 32-33 1.3619425324273848 1104 34-35 1.410292645610923 1104 36-37 1.5429964076263971 1104 38-39 1.6011830672697727 1104 40-41 1.7641337735407916 1104 42-43 1.856991810276888 1104 44-45 1.943953905624582 1104 46-47 1.9557375264936354 1104 48-49 1.945425316689306 1104 50-51 2.1268635088927965 1104 52-53 2.1764851395010147 1104 54-55 2.4392264209693337 1104 56-57 2.7901132032306144 1104 58-59 2.8114547742607883 1104 60-61 3.0650937553488475 1104 62-63 3.1756513374367934 1104 64-65 3.2364943920733182 1104 66-67 3.593312012276037 1104 68-69 3.856954342117664 1104 70-71 3.8029996855674923 1104 72-73 4.207756793703016 1104 74-75 4.11294449459724 1104 76-77 4.274965070477556 1104 78-79 4.6762423432631195 1104 80-81 4.734740885225314 1104 82-83 4.855560382238242 1104 84-85 4.9494887070125095 1104 86-87 4.926210427050915 1104 88-89 5.02446174701168 1104 90-91 4.856027910181661 1104 92-93 4.433374859376331 1104 94-95 4.487007703916971 1104 96-97 4.381045829898797 1104 98-99 3.9809503623794136 1104 100-101 2.739831954381086 1105 1 0.6762894587741535 1105 2 0.4865967782878222 1105 3 0.5163673178177497 1105 4 0.4363086258504012 1105 5 0.4663533811698386 1105 6 0.5912079932310306 1105 7 0.4748557105641851 1105 8 0.43805239409031316 1105 9 0.621838718896953 1105 10-11 0.7750862119102635 1105 12-13 0.8019292023803146 1105 14-15 0.8314299400522458 1105 16-17 0.8798232555000496 1105 18-19 0.8701718067860398 1105 20-21 0.8327571141829608 1105 22-23 0.77093199447296 1105 24-25 0.9384204344640423 1105 26-27 0.9129251955674107 1105 28-29 0.891665354445287 1105 30-31 1.1164195149667009 1105 32-33 1.028258312187937 1105 34-35 1.0920756173243333 1105 36-37 1.3308344003493175 1105 38-39 1.4758023124816901 1105 40-41 1.5595589367699816 1105 42-43 1.5779951962748342 1105 44-45 1.7113700375833076 1105 46-47 1.917390565635614 1105 48-49 1.6944731868907716 1105 50-51 1.6786842841173168 1105 52-53 1.7442291382421935 1105 54-55 2.0196868525820904 1105 56-57 2.2552151328414887 1105 58-59 2.154849455738038 1105 60-61 2.4902205602916645 1105 62-63 2.435882179740208 1105 64-65 2.452855629293264 1105 66-67 2.4988928381642523 1105 68-69 2.943396606287667 1105 70-71 2.835891751298206 1105 72-73 2.8649371296608344 1105 74-75 2.9951994759771665 1105 76-77 3.060819884403081 1105 78-79 3.274917811970319 1105 80-81 3.290130750931546 1105 82-83 3.5094800196664373 1105 84-85 3.36976453083183 1105 86-87 3.573198287578105 1105 88-89 3.5324668250372735 1105 90-91 3.160865584313841 1105 92-93 2.9379014218793653 1105 94-95 3.0101072324415252 1105 96-97 3.0168643167093343 1105 98-99 2.5674057392780885 1105 100-101 1.8247458571759418 1106 1 0.3831475130006261 1106 2 0.33144456970311964 1106 3 0.17361369462934562 1106 4 0.31492314634135 1106 5 0.2521588038173661 1106 6 -0.02208858814335457 1106 7 0.2950900909816312 1106 8 -0.0708894268652358 1106 9 0.4112118552952566 1106 10-11 0.44752570986463525 1106 12-13 0.6033646090788736 1106 14-15 0.7338725584559569 1106 16-17 0.2559379493492955 1106 18-19 0.6577385497303609 1106 20-21 0.7552763569539209 1106 22-23 0.6583999231880284 1106 24-25 0.8748950201377141 1106 26-27 0.7450484879881856 1106 28-29 0.7813869410204148 1106 30-31 0.5085698512817984 1106 32-33 0.6817584162029178 1106 34-35 0.5320017666898451 1106 36-37 0.6928307944967358 1106 38-39 0.7311920219331753 1106 40-41 0.9917671747562551 1106 42-43 0.47339079990842237 1106 44-45 1.059123314055121 1106 46-47 1.1648664688323436 1106 48-49 1.0816880124923252 1106 50-51 0.9418855184283927 1106 52-53 1.045035254322908 1106 54-55 0.8755307607715359 1106 56-57 1.5756939484576051 1106 58-59 1.5091418071699785 1106 60-61 1.892644109282724 1106 62-63 1.747892442551386 1106 64-65 1.7642192656569016 1106 66-67 1.9598265459515076 1106 68-69 2.122408117278585 1106 70-71 2.024106854273029 1106 72-73 2.0317528844628576 1106 74-75 2.4971965635577753 1106 76-77 2.506518865056293 1106 78-79 2.8436734461149697 1106 80-81 2.6047188516180455 1106 82-83 2.638274832786191 1106 84-85 2.7446882531811845 1106 86-87 2.192708030314396 1106 88-89 2.1389469581764455 1106 90-91 2.1159813876561895 1106 92-93 2.226915706603032 1106 94-95 2.2405610844628843 1106 96-97 1.9651775404802247 1106 98-99 1.5797436492704602 1106 100-101 1.039209139888654 1107 1 0.36296673775293087 1107 2 0.2591458068648258 1107 3 0.2495529021505618 1107 4 0.2888331668221582 1107 5 0.18195195521663976 1107 6 0.3112407145267042 1107 7 0.20081867115530372 1107 8 0.41855577752718176 1107 9 0.46677530423305313 1107 10-11 0.3824058102281498 1107 12-13 0.3295944022302777 1107 14-15 0.4520494642669206 1107 16-17 0.46385782480179927 1107 18-19 0.4910077703260427 1107 20-21 0.6056701023721942 1107 22-23 0.7005850968970151 1107 24-25 0.6087756419682862 1107 26-27 0.8221220753036818 1107 28-29 0.7212069970970205 1107 30-31 0.8130476662592372 1107 32-33 0.8701323458728822 1107 34-35 0.857257398911976 1107 36-37 1.038683241991059 1107 38-39 1.1440371159861868 1107 40-41 1.068529771208766 1107 42-43 1.230333869308886 1107 44-45 1.2324162651808095 1107 46-47 1.0015588561690052 1107 48-49 0.7946544043181341 1107 50-51 1.0662508779005755 1107 52-53 1.241121606245155 1107 54-55 1.4627514169131324 1107 56-57 1.6017877067236164 1107 58-59 1.5636880174647985 1107 60-61 1.6069262607500114 1107 62-63 1.8369911358607354 1107 64-65 1.8993400721926221 1107 66-67 2.060823379361672 1107 68-69 2.006367337042942 1107 70-71 2.3703035456023187 1107 72-73 2.4026338614956373 1107 74-75 2.5673206576032293 1107 76-77 2.7268318964425617 1107 78-79 2.84503492859292 1107 80-81 3.000154125463826 1107 82-83 3.178331816288182 1107 84-85 3.056651387727239 1107 86-87 2.964924038597406 1107 88-89 3.096594008108804 1107 90-91 2.7071092680059525 1107 92-93 2.521006880206217 1107 94-95 2.292458393239402 1107 96-97 2.3319672683348767 1107 98-99 2.01101769154708 1107 100-101 1.1597685455673536 1108 1 0.3194347551702208 1108 2 0.19183916232277554 1108 3 0.18717200332831752 1108 4 0.23060720652669175 1108 5 0.18959195889758007 1108 6 0.2756229075221057 1108 7 0.27201572289447284 1108 8 0.22754283141357234 1108 9 0.3626982205923639 1108 10-11 0.15643061570048644 1108 12-13 0.0747167863134095 1108 14-15 0.055417381918942965 1108 16-17 0.29802450729263086 1108 18-19 0.31295992681874196 1108 20-21 0.2819047158073431 1108 22-23 0.34693249036509144 1108 24-25 0.324747213979677 1108 26-27 0.27593499278773237 1108 28-29 0.4373025088285658 1108 30-31 0.5372376029674513 1108 32-33 0.6599528560341881 1108 34-35 0.6024139631889938 1108 36-37 0.711183669243475 1108 38-39 0.6578801675471553 1108 40-41 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2316 28-29 -1.8165602156223954 2316 30-31 -1.6193180168234633 2316 32-33 -1.9678613122211601 2316 34-35 -1.7290571182820855 2316 36-37 -1.8503057709224322 2316 38-39 -2.0023199485353125 2316 40-41 -1.7466629549782269 2316 42-43 -1.4236131704651172 2316 44-45 -1.837151270806423 2316 46-47 -1.312359818720033 2316 48-49 -1.2082031138118445 2316 50-51 -1.0732051651405534 2316 52-53 -1.1456637450717473 2316 54-55 -1.0840281063960262 2316 56-57 -1.024617331201501 2316 58-59 -0.931058521617306 2316 60-61 -1.133814958480695 2316 62-63 -1.0864320004687116 2316 64-65 -1.1683735544359202 2316 66-67 -1.031182991253683 2316 68-69 -0.9150823870597478 2316 70-71 -1.0820590942842188 2316 72-73 -0.9290186083738483 2316 74-75 -0.681359858476906 2316 76-77 -0.80574314992311 2316 78-79 -0.6444025251131755 2316 80-81 -0.4570260949095122 2316 82-83 -0.5823890623342258 2316 84-85 -0.5210200935054239 2316 86-87 -0.3845189990865485 2316 88-89 -0.6277744569145369 2316 90-91 -0.7006361686352793 2316 92-93 -0.6263191982623066 2316 94-95 -0.5542947891973373 2316 96-97 -0.2839092439326478 2316 98-99 -0.49377146663595894 2316 100-101 -0.19719025357595754 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14651.0 3 6026.0 4 6653.0 5 7193.0 6 8651.0 7 10324.0 8 12984.0 9 16145.0 10 19997.0 11 23927.0 12 27712.0 13 31726.0 14 35395.0 15 38994.0 16 43615.0 17 48053.0 18 49122.0 19 50945.0 20 55253.0 21 59959.0 22 65183.0 23 71488.0 24 77814.0 25 84240.0 26 90552.0 27 97948.0 28 105245.0 29 113317.0 30 122309.0 31 132289.0 32 144463.0 33 156422.0 34 168536.0 35 173924.0 36 159542.0 37 101742.0 38 22855.0 39 296.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.34102935951536 18.19877825754717 14.481217375874492 23.97897500706298 2 15.617891477920809 19.39534788984846 40.346138178263 24.640622453967737 3 19.555720863838076 23.224641326971046 28.951636327934516 28.26800148125636 4 13.172571449989997 16.027596895308534 36.09605134124187 34.703780313459596 5 14.341780389577629 36.646711418257034 33.86780962114368 15.143698571021655 6 33.57986700954012 35.03629142505321 17.021580898726693 14.362260666679976 7 29.549351720766325 31.564201542018477 20.166220221423263 18.72022651579194 8 26.419534294403668 35.13626706266513 19.418224484682558 19.025974158248644 9 26.376549325841598 16.845445479048454 19.690900735593672 37.087104459516276 10-11 25.328595782525966 26.083044569657414 27.09688668927616 21.491472958540456 12-13 26.18155435576754 23.272338866267543 27.348377281843973 23.197729496120946 14-15 22.92714253591261 24.94555671063952 25.406399907448446 26.72090084599943 16-17 22.825000865596316 27.98841526626761 26.22088795924976 22.965695908886314 18-19 22.831500650453567 26.61604663956766 28.211618300363916 22.34083440961486 20-21 23.20385786487695 26.015146120258027 28.388362851795513 22.392633163069508 22-23 23.029061110420812 25.793461810315506 28.265682957482824 22.91179412178086 24-25 22.88543916846002 26.021172294712347 28.301555470629893 22.791833066197746 26-27 22.536340805161625 26.372727178283984 28.128977902696413 22.961954113857978 28-29 22.635652346076537 26.735919018826788 28.06160360517441 22.566825029922263 30-31 22.6834768619113 26.26669732991421 28.072596936024556 22.977228872149944 32-33 22.513775778217152 26.23790539325307 28.02477959671438 23.223539231815394 34-35 23.155009641623295 26.401915655197545 27.719193399332674 22.723881303846486 36-37 23.209814982183854 25.958021400559062 27.832154043612228 23.000009573644856 38-39 22.70796607242358 26.298474366953524 27.849503403510717 23.144056157112182 40-41 23.137330532187786 26.134516896072235 27.851535484480607 22.876617087259376 42-43 22.89047439251438 26.15186947006734 27.69360135648945 23.264054780928827 44-45 22.80899757353473 26.473173965239333 27.7989628210128 22.918865640213138 46-47 23.1716400051731 26.607576555265105 27.191240759956948 23.029542679604848 48-49 23.05669492112241 26.522359851246602 27.283397214743367 23.137548012887617 50-51 23.218525961484417 26.810453182492445 27.114072337482597 22.85694851854054 52-53 23.237833138615763 26.553686352860822 27.17932134019722 23.029159168326196 54-55 22.875573555633572 26.70435523508755 27.342424422615913 23.077646786662964 56-57 23.001401067906993 26.82153082960326 27.194585524198967 22.982482578290778 58-59 22.973583599330045 26.40915130503841 27.28781098706416 23.329454108567386 60-61 22.80066826483293 26.663351408414567 27.378555247923487 23.15742507882902 62-63 22.766705529562106 26.636961159166468 27.54227994521859 23.054053366052838 64-65 22.620793104262834 26.588187905905315 27.378901435824503 23.41211755400735 66-67 22.567937214571657 26.677636997968364 27.223452046002084 23.530973741457895 68-69 22.91725721528226 26.357997087169867 27.428335907310746 23.29640979023713 70-71 22.82400040078871 26.361673520941004 27.16764983449137 23.646676243778916 72-73 22.82850305542324 26.408114614366223 27.131286772350144 23.632095557860392 74-75 22.81859198699901 26.765667527765082 26.882562400784458 23.533178084451453 76-77 22.99879406538438 26.371867716181395 26.966730296246126 23.662607922188098 78-79 23.012864583418185 26.424386258445104 26.94459423574127 23.618154922395437 80-81 23.104376420064316 26.516379729754775 26.731696965784458 23.647546884396455 82-83 23.026160504942528 26.50724257235903 26.690121928395605 23.77647499430284 84-85 23.05369076948203 26.378313697587387 26.746965036058718 23.82103049687187 86-87 23.177536941639886 26.30456942926831 26.58507053966581 23.932823089425995 88-89 23.09642421530673 26.268393722657496 26.48924160827931 24.145940453756463 90-91 23.019946118655056 26.302361642120246 26.456546937812814 24.221145301411884 92-93 23.070425174016286 25.893337895024093 26.6627159166351 24.373521014324524 94-95 22.90752792590079 25.77948731944347 26.690513442273843 24.6224713123819 96-97 23.086120869459133 25.57267851734843 26.567578559934628 24.773622053257803 98-99 22.96042821609077 25.651156378133184 26.22485499197828 25.163560413797764 100-101 23.88609862088158 24.69081712435104 26.40751323409335 25.01557102067403 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 227.0 1 187.5 2 201.5 3 265.0 4 234.5 5 212.5 6 347.0 7 686.5 8 1131.0 9 1405.5 10 1310.5 11 1147.0 12 1244.0 13 1366.5 14 1503.5 15 1689.5 16 1835.5 17 2089.5 18 2348.5 19 2573.0 20 2934.5 21 3463.5 22 4260.5 23 5312.0 24 6584.5 25 8339.0 26 10731.0 27 13656.5 28 16701.5 29 19695.0 30 23180.5 31 28180.0 32 34458.0 33 38661.5 34 41396.0 35 45034.0 36 49732.5 37 54953.0 38 59148.0 39 62811.0 40 66802.5 41 71791.5 42 76019.0 43 79517.5 44 82901.0 45 87028.5 46 90233.0 47 91940.5 48 93528.5 49 93827.5 50 93352.0 51 91978.5 52 90547.5 53 90541.0 54 90454.0 55 85946.0 56 78728.5 57 72151.5 58 66530.5 59 59128.0 60 49903.5 61 42188.5 62 35623.0 63 28659.5 64 22262.5 65 17284.5 66 13136.5 67 10217.5 68 7884.0 69 6011.0 70 4679.5 71 3542.5 72 2692.0 73 2079.5 74 1571.0 75 1204.5 76 875.5 77 583.0 78 372.0 79 238.5 80 146.0 81 88.5 82 62.0 83 39.5 84 25.5 85 15.5 86 9.5 87 7.5 88 3.0 89 2.5 90 3.5 91 2.0 92 1.5 93 1.5 94 1.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39254894135182794 2 0.028100297700255343 3 0.033272381479867566 4 0.0411730448912437 5 0.06104687862707647 6 0.05412361687483965 7 0.037263438254686435 8 0.038077939637302535 9 0.035593710420323436 10-11 0.03732452585838265 12-13 0.028690811202652017 14-15 0.026145494381976712 16-17 0.021787911984980594 18-19 0.027061808437419826 20-21 0.03534936000553861 22-23 0.034005432724222046 24-25 0.04300567300212992 26-27 0.03724307572012103 28-29 0.03241715502812066 30-31 0.03536972254010401 32-33 0.029668212861791336 34-35 0.03129721562702353 36-37 0.033985070189656644 38-39 0.015394076131444232 40-41 0.012380421015764675 42-43 0.013398547744034796 44-45 0.011097581338144322 46-47 0.01964984585561334 48-49 0.01710452903493804 50-51 0.011240119080102138 52-53 0.00914277801986569 54-55 0.007819213273114531 56-57 0.008959515208777066 58-59 0.004174319585907497 60-61 0.007167612167021654 62-63 0.011301206683798345 64-65 0.007167612167021654 66-67 0.0032376429958989855 68-69 0.005945860093097508 70-71 0.013479997882296405 72-73 0.010812505854228688 74-75 0.011912082720760418 76-77 0.005416434194397045 78-79 0.009040965347038677 80-81 0.011219756545536735 82-83 0.01301165958729215 84-85 0.007697038065722116 86-87 0.0025656793552407053 88-89 0.01655474060167217 90-91 0.026532382538719358 92-93 0.01058851797400926 94-95 0.02081051032584128 96-97 0.01912041995691288 98-99 0.011443744425756162 100-101 0.12288789610220363 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2455490.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.22949802058123 #Duplication Level Percentage of deduplicated Percentage of total 1 81.71638157205831 50.85169405287463 2 10.846466778013808 13.499403657854206 3 3.2303861473117883 6.030759250795557 4 1.3457396430403519 3.349788098111891 5 0.7455176933947654 2.3196595912708915 6 0.4527115792151756 1.6903208593598982 7 0.2930595505267145 1.2765864103590225 8 0.21022701060672655 1.04658570723392 9 0.16473169864452183 0.922605382325363 >10 0.8977671294802321 10.260589960893482 >50 0.06160837583239277 2.6330007515749956 >100 0.03157120670221716 3.4753701103163683 >500 0.0025103685617003098 1.1266265847277812 >1k 0.0012551842808478592 1.3060728551999248 >5k 6.606233057093996E-5 0.21093672710200861 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5131 0.2089603297101597 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4212 0.17153399117895002 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2737 0.11146451421101289 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.072506913080485E-5 0.0 5 0.0 0.0 0.0 4.072506913080485E-5 0.0 6 0.0 0.0 0.0 4.072506913080485E-5 0.0 7 0.0 0.0 0.0 4.072506913080485E-5 0.0 8 0.0 0.0 0.0 4.072506913080485E-5 0.0 9 0.0 0.0 0.0 4.072506913080485E-5 0.0 10-11 0.0 0.0 0.0 4.072506913080485E-5 0.0 12-13 0.0 0.0 0.0 1.0181267282701213E-4 4.072506913080485E-5 14-15 0.0 0.0 0.0 1.2217520739241455E-4 4.072506913080485E-5 16-17 0.0 0.0 0.0 1.2217520739241455E-4 6.108760369620727E-5 18-19 0.0 0.0 0.0 2.0362534565402426E-4 8.14501382616097E-5 20-21 0.0 0.0 0.0 2.647129493502315E-4 8.14501382616097E-5 22-23 0.0 0.0 0.0 3.8688815674264604E-4 8.14501382616097E-5 24-25 0.0 0.0 0.0 6.923261752236824E-4 8.14501382616097E-5 26-27 0.0 0.0 0.0 0.001058851797400926 8.14501382616097E-5 28-29 2.0362534565402424E-5 0.0 0.0 0.0033801807378568026 8.14501382616097E-5 30-31 4.072506913080485E-5 0.0 0.0 0.01050706783574765 8.14501382616097E-5 32-33 6.108760369620727E-5 0.0 0.0 0.02533099299936062 8.14501382616097E-5 34-35 8.14501382616097E-5 0.0 0.0 0.046670929223902355 8.14501382616097E-5 36-37 8.14501382616097E-5 0.0 0.0 0.08444343084272385 8.14501382616097E-5 38-39 8.14501382616097E-5 0.0 0.0 0.14178432817889708 8.14501382616097E-5 40-41 8.14501382616097E-5 0.0 0.0 0.20087640348769492 8.14501382616097E-5 42-43 8.14501382616097E-5 0.0 0.0 0.2594594154323577 8.14501382616097E-5 44-45 8.14501382616097E-5 0.0 0.0 0.3387103999609039 8.14501382616097E-5 46-47 8.14501382616097E-5 0.0 0.0 0.4355342518193924 8.14501382616097E-5 48-49 8.14501382616097E-5 0.0 0.0 0.5356161092083453 8.14501382616097E-5 50-51 8.14501382616097E-5 0.0 0.0 0.6448000195480332 8.14501382616097E-5 52-53 8.14501382616097E-5 0.0 0.0 0.7532305161088011 8.14501382616097E-5 54-55 8.14501382616097E-5 0.0 0.0 0.8729214942842365 1.0181267282701213E-4 56-57 8.14501382616097E-5 0.0 0.0 1.0146854599285682 1.2217520739241455E-4 58-59 8.14501382616097E-5 0.0 0.0 1.1717009639623863 1.2217520739241455E-4 60-61 8.14501382616097E-5 0.0 0.0 1.3440698190585179 1.2217520739241455E-4 62-63 8.14501382616097E-5 0.0 0.0 1.5118367413428686 1.2217520739241455E-4 64-65 8.14501382616097E-5 0.0 0.0 1.6861196746881477 1.2217520739241455E-4 66-67 8.14501382616097E-5 0.0 0.0 1.8934713641676408 1.2217520739241455E-4 68-69 8.14501382616097E-5 0.0 0.0 2.1255635331440974 1.2217520739241455E-4 70-71 1.0181267282701213E-4 0.0 0.0 2.3697306851178377 1.2217520739241455E-4 72-73 1.2217520739241455E-4 0.0 0.0 2.6175427307787853 1.2217520739241455E-4 74-75 1.2217520739241455E-4 0.0 0.0 2.8652936888360365 1.2217520739241455E-4 76-77 1.2217520739241455E-4 0.0 0.0 3.1355859726571884 1.2217520739241455E-4 78-79 1.629002765232194E-4 0.0 0.0 3.438641574594073 1.2217520739241455E-4 80-81 1.629002765232194E-4 0.0 0.0 3.7528354829382327 1.2217520739241455E-4 82-83 1.629002765232194E-4 0.0 0.0 4.081527515892958 1.2217520739241455E-4 84-85 1.629002765232194E-4 0.0 0.0 4.41152275105987 1.2217520739241455E-4 86-87 1.629002765232194E-4 0.0 0.0 4.752778467841449 1.2217520739241455E-4 88-89 1.629002765232194E-4 0.0 0.0 5.114294906515604 1.2217520739241455E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3405 0.0 55.628574 1 GTATCAA 6045 0.0 44.199387 1 TATCAAC 7765 0.0 34.033607 2 ATCAACG 7805 0.0 33.85918 3 TCAACGC 7950 0.0 33.241627 4 CAACGCA 8070 0.0 32.511734 5 AACGCAG 8355 0.0 31.395037 6 ACGCAGA 9555 0.0 27.2963 7 CGCAGAG 9590 0.0 27.048065 8 GTACATG 7185 0.0 24.171251 1 GCAGAGT 10500 0.0 23.842783 9 TACATGG 7185 0.0 23.21961 2 GAGTACT 6495 0.0 22.37818 12-13 TGGTATC 1505 0.0 21.791502 2 ACATGGG 7445 0.0 21.45108 3 CAGAGTA 10140 0.0 21.126617 10-11 GTACTTT 6885 0.0 20.836739 14-15 AGAGTAC 9655 0.0 20.761158 10-11 GTGGTAT 1810 0.0 20.560825 1 TAAGGTG 1370 0.0 19.775564 5 >>END_MODULE