FastQCFastQC Report
Thu 26 May 2016
SRR938480_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938480_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2455490
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53210.2166980928450126No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48150.19609120786482537No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30710.1250666873007017No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34600.055.3412631
GTATCAA61200.040.7594221
ATCAACG79050.031.064713
TCAACGC80500.030.504544
CAACGCA82550.029.8045445
TATCAAC82750.029.6781312
TGGTATC15900.028.6805932
AACGCAG86500.028.3886176
GTGGTAT18400.026.0809671
ACGCAGA101300.024.0534697
CGCAGAG101950.023.8069348
GGACCGA8000.023.7486976
GTACATG74400.022.2242151
TACATGG72850.022.1046752
GCAGAGT114200.020.9204839
GAGTACT68750.020.65704712-13
ACATGGG76000.020.6242893
AGAGTAC104700.019.80192210-11
GTACTTT73000.019.1616214-15
CAGAGTA110350.019.1323910-11
Produced by FastQC (version 0.11.5)